FastQCFastQC Report
Wed 25 May 2016
SRR1295234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921246
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT193470.39313214580209965No Hit
GGTATCAACGCAGAGTACTTTTTTT169440.3443030484556147No Hit
TATCAACGCAGAGTACTTTTTTTTT109670.222850066832668No Hit
GTGGTATCAACGCAGAGTACATGGG76940.15634251976023958No Hit
ACGCAGAGTACTTTTTTTTTTTTTT76280.15500139598792664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.011.9135531
GCAGCGT5200.011.5123741
GTGGTAT22150.011.3255131
AGCGTCA5500.011.2281373
CAGCGTC5750.010.9050772
TGGTATC22600.010.5936282
TATACTG9200.09.19111255
CTTACAC5500.09.155253
TAGGACT5900.09.0176444
CTAGGAC4250.08.9418653
TCTACAC5750.08.9224263
GTCTAGA4600.08.8825641
GTGTACG1107.132241E-48.6384331
CTAGACT5100.08.5692874
TATCTCG1000.00290415558.5508335
TAAGACG1000.00290468998.5506584
CCGCCCT1451.6909597E-58.5172059
TAGACAG5200.08.4046655
GTCTTAT6000.08.3936771
GTTCTAA4650.08.3783521
Produced by FastQC (version 0.11.5)