FastQCFastQC Report
Wed 25 May 2016
SRR1295233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633852
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG53680.1158431473426428No Hit
GTATCAACGCAGAGTACTTTTTTTT51910.11202343104613614No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51680.11152708373077086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.010.564771
TAGTCCG1304.2193697E-69.5042495
TACACCG800.00450165949.5042495
TCGGTCT1106.848815E-59.4992036
TAGGACC2357.2759576E-129.3023334
TATACTG7550.09.0636545
ACTACGC1054.477121E-49.0513723
GTCTACG850.00740622548.9460481
CGTATAC850.00741413148.9448843
GTCCTAG4750.08.8047941
AGCCTCG4300.08.6195732
GCCGTCC1000.00290856568.54937658
TGGTATC14950.08.4547362
AGCCGCG1356.773455E-58.4476712
AGGCTCG1252.734039E-48.3631952
TAGACAG5800.08.3571855
GTGGTAT16450.08.3206411
TTATCGT1150.00110601968.2648334
ATACCGT1502.591135E-58.2326436
CCGTCCC1050.0045187398.1421749
Produced by FastQC (version 0.11.5)