##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295233_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4633852
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.088824804935506	31.0	30.0	33.0	26.0	34.0
2	30.084433210210424	31.0	30.0	34.0	26.0	34.0
3	30.16340616834547	31.0	30.0	34.0	26.0	34.0
4	33.544642772363034	35.0	35.0	37.0	27.0	37.0
5	33.68749088231562	35.0	35.0	37.0	28.0	37.0
6	33.98499239941198	36.0	35.0	37.0	28.0	37.0
7	33.81122379394076	36.0	35.0	37.0	28.0	37.0
8	33.88560553940868	37.0	35.0	37.0	28.0	37.0
9	35.016439238888076	39.0	35.0	39.0	27.0	39.0
10	34.99468368864608	39.0	35.0	39.0	27.0	39.0
11	35.44750609212379	39.0	35.0	39.0	28.0	39.0
12	35.43896373902317	39.0	35.0	39.0	27.0	39.0
13	35.381653535762474	39.0	35.0	39.0	27.0	39.0
14	36.03339662121276	39.0	36.0	41.0	27.0	41.0
15	36.39870090801346	40.0	36.0	41.0	27.0	41.0
16	36.36621627104189	40.0	36.0	41.0	27.0	41.0
17	36.03197685208764	39.0	36.0	41.0	27.0	41.0
18	35.934988644436636	39.0	35.0	41.0	27.0	41.0
19	36.361957395272874	40.0	36.0	41.0	27.0	41.0
20	36.40366697080528	40.0	36.0	41.0	27.0	41.0
21	36.4364291306671	40.0	36.0	41.0	27.0	41.0
22	36.34854889625305	40.0	36.0	41.0	27.0	41.0
23	36.066400049030484	39.0	35.0	41.0	27.0	41.0
24	36.34928111644481	39.0	36.0	41.0	27.0	41.0
25	35.08966082645713	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.19338465772098345
1101	2	0.038585651147521105
1101	3	-0.11208743568882085
1101	4	-0.04605252032244067
1101	5	-0.34204923512243823
1101	6	-0.3900551337933891
1101	7	-0.3538015936562431
1101	8	-0.3356400362014895
1101	9	-0.053206542332915774
1101	10	0.013002398355347111
1101	11	-0.06730422580718454
1101	12	-0.09603591375451259
1101	13	-0.013825011874210702
1101	14	0.3459567500550378
1101	15	0.0749899314351552
1101	16	-0.01036883180328374
1101	17	0.023956164581768746
1101	18	0.26009784400572755
1101	19	-0.09353693819755904
1101	20	0.01892890604559483
1101	21	-0.07698242798716137
1101	22	-0.05893742423881321
1101	23	0.04100900847581812
1101	24	-0.04414883250903756
1101	25	-0.03215122396944281
1102	1	-0.36620874709398166
1102	2	-0.16253886017352315
1102	3	-0.3814724458645742
1102	4	-0.31888920902051865
1102	5	-0.7270910864394793
1102	6	-0.5985596494777283
1102	7	-0.6254239182039356
1102	8	-0.6529054901839189
1102	9	-0.5624222252445961
1102	10	-0.1411401974524651
1102	11	-0.28821442979457146
1102	12	-0.2597963265218155
1102	13	-0.3685280908139177
1102	14	-0.24581542118809097
1102	15	-0.27550947610912857
1102	16	-0.4600900801195067
1102	17	-0.42346470566846506
1102	18	-0.1245754938199255
1102	19	-0.28933044991881474
1102	20	-0.23162537323612753
1102	21	-0.23511007055187605
1102	22	-0.4294657153765442
1102	23	-0.32226918069360977
1102	24	-0.3525996070417676
1102	25	-0.29791261076518083
1103	1	-0.44304071103123377
1103	2	-0.25160840216924996
1103	3	-0.5422662864334278
1103	4	-0.2893856420828058
1103	5	-0.5816437283703522
1103	6	-0.5633896726451795
1103	7	-0.5269739244379821
1103	8	-0.40852304255232497
1103	9	0.04191879739030924
1103	10	0.08670743278836568
1103	11	-0.029695194620245502
1103	12	-0.15355387140936472
1103	13	-0.1846575502109431
1103	14	-0.18157777740633207
1103	15	-0.19879339777858007
1103	16	-0.1530667744511618
1103	17	-0.16212884134451144
1103	18	0.2235536640232283
1103	19	-0.16452696735819927
1103	20	-0.058629715805189164
1103	21	-0.10980312833714123
1103	22	-0.03508225290018174
1103	23	-2.3591649735338294
1103	24	-1.0296533870485476
1103	25	-0.5925000087128751
1104	1	-0.3464972574240939
1104	2	-0.13135189326251862
1104	3	-0.4162293041210319
1104	4	-0.6797810994455276
1104	5	-0.6106439736953817
1104	6	-0.512036870663529
1104	7	-0.5560861634461318
1104	8	-0.5905828511290991
1104	9	-2.689345699198668
1104	10	-0.9864501365537137
1104	11	-0.6873780213067278
1104	12	-0.5039673128199595
1104	13	-0.4482900219506476
1104	14	-0.2995137671301791
1104	15	-0.3556413642238496
1104	16	-0.346377959471738
1104	17	-0.02903178421863828
1104	18	0.05302672638632089
1104	19	-0.13983735579852663
1104	20	-0.34271919689918917
1104	21	-0.34894877375979405
1104	22	-0.31835879229537767
1104	23	-0.30835769541599234
1104	24	-0.3286597529135591
1104	25	-0.36370686650587203
1105	1	-0.3751503334172064
1105	2	-0.2780529982697715
1105	3	-0.38656876927373673
1105	4	-0.09988388987923713
1105	5	-0.3075831561662241
1105	6	-0.32674754930656036
1105	7	-0.4075570516438347
1105	8	-0.43345731692119926
1105	9	-2.230071573408793
1105	10	-0.7306839675443157
1105	11	-0.5563349279359215
1105	12	-0.4342383586246612
1105	13	-0.4280576794065638
1105	14	-0.4126458681043417
1105	15	-0.36852342117008874
1105	16	-0.33517298937469775
1105	17	-1.6411387767371082
1105	18	-0.555146432249586
1105	19	-0.4322387171525506
1105	20	-0.3597672323505776
1105	21	-0.3740430098534304
1105	22	-0.3575776480266484
1105	23	-0.22195068446007582
1105	24	-0.26617216984537606
1105	25	-0.8950369844795745
1106	1	-0.31691652164442985
1106	2	-0.5323753317171054
1106	3	-0.4497742523820669
1106	4	-0.2906235794976979
1106	5	-0.27344734361478373
1106	6	-0.4519925819516786
1106	7	-0.4224048272468437
1106	8	-0.45382784604612425
1106	9	-3.1262714405161915
1106	10	-0.7351244494750233
1106	11	-0.6058076338665614
1106	12	-0.46884544159791375
1106	13	-0.45659706295293034
1106	14	-0.7468463221691124
1106	15	-0.7016712021774367
1106	16	-0.6554048806518225
1106	17	-0.45332035812700866
1106	18	-0.18216151135898428
1106	19	-0.35572137368512813
1106	20	-0.34417227424824404
1106	21	-0.3205798560674893
1106	22	-0.3351599270876804
1106	23	-0.1865607443319348
1106	24	-0.37410777538756435
1106	25	-0.6086206760885204
1107	1	-0.23743948689706684
1107	2	-0.02050479947818573
1107	3	-0.13637947643360704
1107	4	-1.4335292196005085
1107	5	-0.3007295885377914
1107	6	-0.2182899012426205
1107	7	-0.009147678937893033
1107	8	-0.02297299819223042
1107	9	0.33809493063644425
1107	10	0.5360203340976568
1107	11	0.21726478511383363
1107	12	-0.20924830286364937
1107	13	0.011286577652157348
1107	14	0.3094754179783976
1107	15	-0.4495873166399349
1107	16	-0.7266060815097362
1107	17	-0.029610486715938578
1107	18	-0.006180323080350547
1107	19	0.12624556474938942
1107	20	-0.32600749455907163
1107	21	-0.020830029526017313
1107	22	0.047333049231255586
1107	23	0.2423481165756627
1107	24	-0.01084849308678315
1107	25	-0.18632556807985878
1108	1	0.26176300657142804
1108	2	0.252751192237497
1108	3	0.27628042348001713
1108	4	0.660824472101659
1108	5	0.5446147107718318
1108	6	0.4720686103791607
1108	7	0.4460473620878318
1108	8	0.4758450809283872
1108	9	0.7499446634473159
1108	10	0.8079905663582139
1108	11	0.5525486004598079
1108	12	0.5005674270825011
1108	13	0.4149671407126334
1108	14	0.5508092934420148
1108	15	0.5223421172034008
1108	16	0.5270219383557588
1108	17	0.5313115254056697
1108	18	0.6950174253373902
1108	19	0.4807046726731343
1108	20	0.49019308187272514
1108	21	0.4047855022730644
1108	22	0.49862958294198023
1108	23	0.6868962009509687
1108	24	0.5366915506217396
1108	25	0.6311751796965339
1109	1	0.23742120388870802
1109	2	0.18888911060229674
1109	3	0.34288379697108695
1109	4	0.662377032943688
1109	5	0.5487076886119695
1109	6	0.4932516074464033
1109	7	0.3748408877267053
1109	8	0.4050115572774402
1109	9	0.7983758645626153
1109	10	0.49454093531583965
1109	11	0.501103953297175
1109	12	0.4203324286352057
1109	13	0.40872669788878824
1109	14	0.7557743640548793
1109	15	0.53093923075307
1109	16	0.5851980837015347
1109	17	0.7929525358212857
1109	18	0.5271103187195934
1109	19	0.4887868154800046
1109	20	0.5213052003505396
1109	21	0.4159507545566612
1109	22	0.5112631535906118
1109	23	0.6735647391938926
1109	24	0.4938496547192983
1109	25	0.4274741053284501
1110	1	0.27807576627033725
1110	2	0.2091771945776877
1110	3	0.372386151471094
1110	4	0.5470850514724432
1110	5	0.5206697221659979
1110	6	0.4662970097170529
1110	7	0.4876257231961958
1110	8	0.5111998454446294
1110	9	0.9917366617285381
1110	10	0.9356662554545849
1110	11	0.520249477115641
1110	12	0.4507463206475464
1110	13	0.48854095874079206
1110	14	0.7087872599298635
1110	15	0.45560863704638876
1110	16	0.49295111332890684
1110	17	1.0208178709383589
1110	18	0.9789005708548615
1110	19	0.6009258614896567
1110	20	0.5086484381596605
1110	21	0.5189615701076704
1110	22	0.6293315674449289
1110	23	0.7718371279250036
1110	24	0.558897953796631
1110	25	0.5212872040439152
1111	1	0.13147261845956137
1111	2	0.12888661345017383
1111	3	0.22388019951805305
1111	4	0.44545005381574043
1111	5	0.39049000773894704
1111	6	0.2957745728285843
1111	7	0.17341389586478329
1111	8	0.1561456895267881
1111	9	0.4855886219236041
1111	10	0.3729418752119571
1111	11	0.10791019285370851
1111	12	0.027776607464964798
1111	13	0.1124112706685807
1111	14	0.07254571902213769
1111	15	0.15516564237783825
1111	16	-0.028686729599108673
1111	17	0.3587010728181994
1111	18	0.4156753907916766
1111	19	0.28683794688200237
1111	20	-0.2424469423009512
1111	21	-0.3448712811597332
1111	22	-0.22467962817210463
1111	23	0.11403933407034827
1111	24	0.11643613668735497
1111	25	-0.09452473652822135
1112	1	0.2748458143069179
1112	2	0.20687345604633478
1112	3	0.33109694825772706
1112	4	0.5823238412286287
1112	5	0.6056993271255564
1112	6	0.43728022642213205
1112	7	0.3263406810869043
1112	8	0.42824909375763553
1112	9	-1.1234456589030941
1112	10	0.08615015017795002
1112	11	0.1261821327284096
1112	12	0.20976594281018635
1112	13	0.20718638276097323
1112	14	0.5229442431854494
1112	15	0.426511241116458
1112	16	0.2763161365521043
1112	17	0.3227516284731351
1112	18	0.48190033110100927
1112	19	0.2946208255904992
1112	20	0.3072700724332904
1112	21	0.22763740206141136
1112	22	0.2922722771408459
1112	23	0.11070858255837379
1112	24	0.14136681600611922
1112	25	0.22729031471439498
1113	1	0.33485503851758835
1113	2	0.38167068163938467
1113	3	0.43906778588226913
1113	4	0.6111614580443501
1113	5	0.7464646253776408
1113	6	0.4975320665905443
1113	7	0.5910328632654824
1113	8	0.532864666527125
1113	9	0.36624645277795054
1113	10	0.6929016937867374
1113	11	0.4986006566395531
1113	12	0.3199178174753925
1113	13	0.36669770638820154
1113	14	0.15298942531608617
1113	15	0.25074825152805147
1113	16	0.49173524230289445
1113	17	0.7360161624797357
1113	18	0.6260437667414109
1113	19	0.4215230709944038
1113	20	0.4890244646646522
1113	21	0.4321008777068087
1113	22	0.5186361511547517
1113	23	0.20732769837726295
1113	24	0.34220154858494567
1113	25	0.3417969003851411
1114	1	0.4315795449158095
1114	2	0.3971771176979999
1114	3	0.5096907127272239
1114	4	0.7316645129934116
1114	5	0.7003777496078314
1114	6	0.5594829154234091
1114	7	0.3184169336712799
1114	8	0.3948081392184193
1114	9	0.3201442420307359
1114	10	0.5041143222156421
1114	11	0.5132481246123533
1114	12	0.4644922510045504
1114	13	0.4486738026301751
1114	14	0.7745276784695605
1114	15	0.5169669484384372
1114	16	0.309487758831132
1114	17	0.8525627932587199
1114	18	0.6619129592794835
1114	19	0.5365297141449901
1114	20	0.48529058084924515
1114	21	0.5055265987835327
1114	22	0.2986163678126843
1114	23	0.75890406863207
1114	24	0.4989042744303944
1114	25	0.5016250481496343
1115	1	0.32966562015618806
1115	2	0.31065288101772026
1115	3	0.4138773188915117
1115	4	0.6252751895068371
1115	5	0.7013594522109656
1115	6	0.5219009227966893
1115	7	0.48979862789647655
1115	8	0.49849611605592514
1115	9	0.6712491030572281
1115	10	0.5114766929549575
1115	11	0.40105721962402896
1115	12	0.339772414224484
1115	13	0.19826168519509935
1115	14	0.284857644977933
1115	15	0.26120092808185547
1115	16	0.3753203331452539
1115	17	0.6393012533290161
1115	18	0.7485743950536943
1115	19	0.5488725350970682
1115	20	0.4860413700777997
1115	21	0.33530566125511996
1115	22	-0.30070856340397967
1115	23	0.28089193560627024
1115	24	0.37543452200184646
1115	25	0.361476702517912
1116	1	0.7201290788816586
1116	2	0.6615677928384365
1116	3	0.8565176605689864
1116	4	1.000639429288249
1116	5	1.048555378576431
1116	6	0.8538557705873799
1116	7	0.8469060989863664
1116	8	0.7419769208471365
1116	9	1.011503015236734
1116	10	0.7573885688691817
1116	11	0.5062569684717673
1116	12	0.5847374323019281
1116	13	0.5630029431643564
1116	14	0.9704528206465213
1116	15	0.6521646214001038
1116	16	0.8115805793296715
1116	17	1.092404501748966
1116	18	1.0955639487754425
1116	19	0.7961563647551202
1116	20	0.7918034516056878
1116	21	0.8683754457057589
1116	22	0.8793520857280299
1116	23	1.0634861867033365
1116	24	0.778367621662376
1116	25	0.6931077432818569
1201	1	-0.1619160240900257
1201	2	0.051175206510645666
1201	3	-0.18382752408609804
1201	4	-0.04426307470556168
1201	5	-0.4463582032768443
1201	6	-0.442702033068862
1201	7	-0.45864927585370197
1201	8	-0.4153129251109817
1201	9	0.20216428935321318
1201	10	0.27684349481197046
1201	11	0.144149699371944
1201	12	0.07103845732018499
1201	13	0.10400287810678321
1201	14	0.43135242234958326
1201	15	0.13581809222178265
1201	16	0.10530503519533596
1201	17	0.209046807967745
1201	18	0.39010151591124753
1201	19	0.06518169383802075
1201	20	0.15590010909200913
1201	21	0.10571413906048832
1201	22	0.008329362562470521
1201	23	-0.04620422959285975
1201	24	-0.10630282557231396
1201	25	-0.0868654566111573
1202	1	-0.4946004889105424
1202	2	-0.43341311438017627
1202	3	-0.5423277025603213
1202	4	-0.34532482946474374
1202	5	-0.8352450743732405
1202	6	-0.6259594581961849
1202	7	-0.6394337671809325
1202	8	-0.6932748766594443
1202	9	-0.026536768109671982
1202	10	0.07705065229107078
1202	11	-0.2462830494116801
1202	12	-0.28855651224471757
1202	13	-0.2824562730421505
1202	14	-0.31365663486106854
1202	15	-0.3137147276130108
1202	16	-0.22717082239564235
1202	17	0.1351501976277092
1202	18	0.1926973380556305
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2211	10	-0.021878556508845293
2211	11	-0.010171394872017459
2211	12	-0.07284635650588456
2211	13	-0.014323306037987038
2211	14	0.2970052718853182
2211	15	0.1412980869828786
2211	16	0.17281597871374288
2211	17	0.4043973991215495
2211	18	0.4584208835477952
2211	19	0.23221787461833543
2211	20	0.3473245652754713
2211	21	0.14229985543857282
2211	22	0.1301147164746368
2211	23	-1.8345692609563997
2211	24	-0.48724497995235794
2211	25	-0.29789224284582616
2212	1	-0.037941530810890356
2212	2	-1.6271763447332184
2212	3	-0.36185779539766116
2212	4	0.1961227539365069
2212	5	0.2255486042147865
2212	6	0.31231851159832047
2212	7	0.3256841083662465
2212	8	0.2758643622228476
2212	9	0.7137906650543471
2212	10	0.55057512436683
2212	11	0.3244135604467573
2212	12	0.18028068399551955
2212	13	0.08928746635351814
2212	14	0.3737566247111346
2212	15	0.1730804629842595
2212	16	0.1526423302416049
2212	17	0.48520283421378707
2212	18	0.7238508169359434
2212	19	0.28565349867903933
2212	20	0.28222424368489385
2212	21	0.26188638890533866
2212	22	0.31504124674939504
2212	23	0.46853438889198173
2212	24	0.25808361957795967
2212	25	0.20889070315678993
2213	1	0.4259730270453268
2213	2	0.3426657881222681
2213	3	0.37666206721034357
2213	4	0.6466777636934395
2213	5	0.6449266279454164
2213	6	0.5140718705534937
2213	7	0.44248123608018375
2213	8	0.4232559560385809
2213	9	0.7341177210987482
2213	10	0.7113791572834529
2213	11	0.4477368729037181
2213	12	0.44793109590516167
2213	13	0.20203709733180375
2213	14	-1.1866737067480386
2213	15	-0.17604446309670863
2213	16	0.13021373839999484
2213	17	0.5436874786037933
2213	18	0.3735394378528838
2213	19	0.27524165681854385
2213	20	0.366615824971106
2213	21	0.3665696971008998
2213	22	0.3549065982410795
2213	23	0.7032936157015328
2213	24	0.4179151256213274
2213	25	0.21846344382608152
2214	1	0.39038840437986977
2214	2	0.44593502158992493
2214	3	0.43631624684049086
2214	4	0.6316805636776195
2214	5	0.5831678807055383
2214	6	0.36228874623036944
2214	7	0.31481190719620855
2214	8	0.2299891845160147
2214	9	0.3044428891934672
2214	10	0.1951972897003813
2214	11	0.061190114443490984
2214	12	0.027242542163961048
2214	13	-0.0776664487620593
2214	14	0.22951430931966144
2214	15	0.0791323441049272
2214	16	0.20415041411079926
2214	17	0.209348804252798
2214	18	0.4493848481111584
2214	19	0.1829380033175454
2214	20	0.26883930580429194
2214	21	0.3390733676951996
2214	22	0.3212900009043196
2214	23	0.3336220238690686
2214	24	0.39067131779591335
2214	25	0.19022194869830855
2215	1	0.3959662369534449
2215	2	0.2794056012292536
2215	3	0.4514042292678724
2215	4	0.6490558003037705
2215	5	0.6127895342852767
2215	6	0.5528255426692823
2215	7	0.5643337978103418
2215	8	0.4877949268532191
2215	9	0.7036876786030035
2215	10	0.7213035234830372
2215	11	0.4177691985553196
2215	12	0.27515886286760605
2215	13	0.2050108968044384
2215	14	0.508076057805269
2215	15	0.33053751297047285
2215	16	0.36985684234456784
2215	17	0.5211698489376104
2215	18	0.7384863156643249
2215	19	0.353055909191319
2215	20	-0.5110381531501531
2215	21	-0.032004684494239655
2215	22	-0.6846223679699364
2215	23	0.2669123635764947
2215	24	0.20449461432841787
2215	25	0.3782041164764536
2216	1	0.37307395309103697
2216	2	-0.004610831868774312
2216	3	0.44376402050499664
2216	4	0.6439003494802265
2216	5	0.6233145639581252
2216	6	0.5617064959000828
2216	7	0.5616997443155398
2216	8	0.5408075102101151
2216	9	0.7820283184910224
2216	10	0.5466082755039849
2216	11	0.4055527333745985
2216	12	0.07477548674932422
2216	13	0.32115844156792406
2216	14	0.41472241151480205
2216	15	0.33333138257005146
2216	16	0.4794370254661757
2216	17	0.5566833404386102
2216	18	0.8420197549897068
2216	19	0.5378364643885831
2216	20	-0.7573389479676962
2216	21	-0.9738829971501346
2216	22	-0.8984792157846968
2216	23	-1.421255667451092
2216	24	-1.2101078716110152
2216	25	-0.7117332718184173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9112.0
3	1198.0
4	1099.0
5	788.0
6	911.0
7	1299.0
8	683.0
9	1497.0
10	3410.0
11	6261.0
12	10504.0
13	15723.0
14	20993.0
15	25395.0
16	29047.0
17	30813.0
18	31552.0
19	31928.0
20	31697.0
21	31992.0
22	32640.0
23	33827.0
24	35280.0
25	37927.0
26	41237.0
27	46602.0
28	53722.0
29	65190.0
30	80306.0
31	101157.0
32	132330.0
33	177998.0
34	243561.0
35	348100.0
36	515393.0
37	847959.0
38	1554721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.346806964281505	21.417134425034863	13.621531414927912	23.614527195755716
2	15.579498281914855	22.21326762525098	39.53889788917258	22.668336203661585
3	18.105550716004537	26.539967870578064	30.364750572571936	24.989730840845464
4	12.888850767857212	18.012988875156655	38.299485755946556	30.79867460103958
5	13.199770991423875	37.72424568729759	35.3022612105785	13.773722110700042
6	30.058472061483897	38.833070438210264	18.14903068023978	12.959426820066062
7	25.225525439304196	32.78246273649803	24.07697074654323	17.915041077654546
8	23.775438693755074	36.66476667824563	21.706710799528413	17.853083828470893
9	24.62761030075706	17.014446923072594	22.333442901112264	36.02449987505808
10	14.655588440684603	30.053518237095233	34.53882216557817	20.752071156641996
11	31.578605638280766	25.683856402762412	24.34584848968207	18.391689469274752
12	20.504012884188246	27.490735917013215	31.971923451846905	20.03332774695163
13	26.1521420642576	24.495416974770414	27.062924165782043	22.289516795189947
14	20.681038183592207	23.828044916168075	28.323796654083633	27.167120246156085
15	22.300861956945468	31.306514488673283	24.588534673192704	21.804088881188548
16	21.91800867067789	31.212881853138768	25.95912988439962	20.909979591783724
17	20.48860460407131	30.21526020398771	28.4809856554559	20.815149536485084
18	20.52819332491426	30.07036125880815	29.15752032366309	20.243925092614504
19	20.988236822588764	29.16193992280584	29.37927450481516	20.470548749790233
20	22.001800010909157	28.88359496551061	28.77837787761484	20.33622714596539
21	22.35142795893576	29.1508467713122	27.849584139034633	20.648141130717413
22	21.56708989580914	28.758962407183475	29.04976785993722	20.624179837070162
23	21.92447419400073	29.098984310215588	28.282381495645186	20.694160000138496
24	21.659353895684987	28.388250554844053	28.73537987009332	21.217015679377642
25	22.00427005095877	28.742967220009795	28.214719964880203	21.03804276415123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12328.0
1	12328.0
2	10495.5
3	8663.0
4	8663.0
5	8663.0
6	9386.5
7	10110.0
8	10110.0
9	10110.0
10	14684.5
11	19259.0
12	19259.0
13	19259.0
14	31783.0
15	44307.0
16	44307.0
17	44307.0
18	70942.0
19	97577.0
20	97577.0
21	97577.0
22	140532.0
23	183487.0
24	183487.0
25	183487.0
26	237235.0
27	290983.0
28	290983.0
29	290983.0
30	347220.0
31	403457.0
32	403457.0
33	403457.0
34	449642.0
35	495827.0
36	495827.0
37	495827.0
38	525029.0
39	554231.0
40	554231.0
41	554231.0
42	550676.0
43	547121.0
44	547121.0
45	547121.0
46	526480.0
47	505839.0
48	505839.0
49	505839.0
50	479792.0
51	453745.0
52	453745.0
53	453745.0
54	412468.0
55	371191.0
56	371191.0
57	371191.0
58	321236.5
59	271282.0
60	271282.0
61	271282.0
62	224601.0
63	177920.0
64	177920.0
65	177920.0
66	138711.0
67	99502.0
68	99502.0
69	99502.0
70	74346.0
71	49190.0
72	49190.0
73	49190.0
74	35735.5
75	22281.0
76	22281.0
77	22281.0
78	15957.5
79	9634.0
80	9634.0
81	9634.0
82	6820.5
83	4007.0
84	4007.0
85	4007.0
86	2715.5
87	1424.0
88	1424.0
89	1424.0
90	915.0
91	406.0
92	406.0
93	406.0
94	236.0
95	66.0
96	66.0
97	66.0
98	40.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14331489223220767
2	0.2807599379522695
3	0.3378398792192759
4	0.3663906400118087
5	0.3761665240926987
6	0.33248796034055467
7	0.3350560181896185
8	0.3513275780063757
9	0.3389404754403032
10	0.36919608135952553
11	0.28919784231347917
12	0.3340201629227692
13	0.30635419517067014
14	0.3165616856127472
15	0.35503939271258556
16	0.3052320186315834
17	0.35428408158050795
18	0.3231652629389113
19	0.3372356303136138
20	0.29953481466391246
21	0.33928576052925297
22	0.29308229956416393
23	0.2752785371651922
24	0.3136915033108524
25	0.30719582757498515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4633852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.47368459289554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52786977019537	66.2615920647836
2	10.56106297182675	16.36408923290027
3	2.2264319557498986	5.174696613219336
4	0.6708116152854677	2.0788099001550844
5	0.3015388317532408	1.1680662171880385
6	0.16588169765716168	0.7710879794415
7	0.11276886499213198	0.6115633634809476
8	0.07789500191472985	0.4827850247763819
9	0.056698920276563834	0.3953406839637812
>10	0.2764038557534679	3.8001448726633407
>50	0.015010513879933985	0.8068234837508298
>100	0.006704629613575353	0.9053378537157282
>500	5.304867740906629E-4	0.2956297756752373
>1k	3.071234003014299E-4	0.5438438922861416
>5k	8.376092735444893E-5	0.34018904199987626
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5368	0.1158431473426428	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5191	0.11202343104613614	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5168	0.11152708373077086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.316063611871937E-5	0.0
4	0.0	0.0	0.0	4.316063611871937E-5	0.0
5	0.0	0.0	0.0	4.316063611871937E-5	0.0
6	0.0	0.0	0.0	6.474095417807906E-5	0.0
7	0.0	0.0	0.0	6.474095417807906E-5	0.0
8	0.0	0.0	0.0	6.474095417807906E-5	0.0
9	0.0	0.0	0.0	1.0790159029679843E-4	0.0
10	0.0	0.0	0.0	1.0790159029679843E-4	0.0
11	0.0	0.0	0.0	1.0790159029679843E-4	0.0
12	0.0	0.0	0.0	1.0790159029679843E-4	0.0
13	0.0	0.0	0.0	1.0790159029679843E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3050	0.0	10.56477	1
TAGTCCG	130	4.2193697E-6	9.504249	5
TACACCG	80	0.0045016594	9.504249	5
TCGGTCT	110	6.848815E-5	9.499203	6
TAGGACC	235	7.2759576E-12	9.302333	4
TATACTG	755	0.0	9.063654	5
ACTACGC	105	4.477121E-4	9.051372	3
GTCTACG	85	0.0074062254	8.946048	1
CGTATAC	85	0.0074141314	8.944884	3
GTCCTAG	475	0.0	8.804794	1
AGCCTCG	430	0.0	8.619573	2
GCCGTCC	100	0.0029085656	8.5493765	8
TGGTATC	1495	0.0	8.454736	2
AGCCGCG	135	6.773455E-5	8.447671	2
AGGCTCG	125	2.734039E-4	8.363195	2
TAGACAG	580	0.0	8.357185	5
GTGGTAT	1645	0.0	8.320641	1
TTATCGT	115	0.0011060196	8.264833	4
ATACCGT	150	2.591135E-5	8.232643	6
CCGTCCC	105	0.004518739	8.142174	9
>>END_MODULE