FastQCFastQC Report
Wed 25 May 2016
SRR1295233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633852
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT139180.3003548667501681No Hit
GGTATCAACGCAGAGTACTTTTTTT122030.2633446212783662No Hit
TATCAACGCAGAGTACTTTTTTTTT79220.17095927966624744No Hit
GTGGTATCAACGCAGAGTACATGGG58490.1262232803291948No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54590.11780695628604453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG604.0946473E-412.6667039
GCAGCGT4100.012.0523261
GGTATCA42350.012.0047131
CGACCGC658.028498E-411.69234110
AGCGTCA4450.011.5282483
TGAACCG1053.4680888E-610.8577595
CAGCGTC4850.010.5774642
GTGGTAT17500.010.5345291
TGGTATC16400.010.1952612
ACGTAGC1106.820559E-59.5027961
AGTACCG800.0045161839.500545
ATCGACC800.00451819379.5000288
CGTAGCA1151.11138776E-49.0870812
GTCTAGG4700.08.491861
GTCCTAC3050.08.4123111
ACCGTGT3200.08.3125248
GTATCAA75600.08.2583821
TATACTG8900.08.2195685
GTATAGG4200.08.1452531
TTCAACG1050.00451478368.1429682
Produced by FastQC (version 0.11.5)