FastQCFastQC Report
Wed 25 May 2016
SRR1295232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868073
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA458151.597414012823244No Hit
GCTTATGTACTCTGCGTTGATACCA269780.9406315669092105No Hit
CTTATGTACTCTGCGTTGATACCAC179980.6275293550756902No Hit
GTATCAACGCAGAGTACTTTTTTTT171200.5969164662126801No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA124000.43234603861198795No Hit
GAGTACATAAGCAGTGGTATCAACG123600.43095137397130406No Hit
GGTATCAACGCAGAGTACTTTTTTT117860.4109379363774911No Hit
TATGTACTCTGCGTTGATACCACTG109490.3817545787711819No Hit
GTGGTATCAACGCAGAGTACATGGG109360.38130131276295964No Hit
TATCAACGCAGAGTACTTTTTTTTT107320.3741885230954721No Hit
CCCATGTACTCTGCGTTGATACCAC102770.35832421280769355No Hit
GCGTTGATACCACTGCTTATGTACT90480.31547314172268276No Hit
CATGTACTCTGCGTTGATACCACTG79320.2765619982476039No Hit
CATAAGCAGTGGTATCAACGCAGAG77420.2699373412043557No Hit
GTACTGGTTCACTATCGGTCAGTCA67820.23646538982794368No Hit
CTGCTTATGTACTCTGCGTTGATAC67330.23475692564310602No Hit
GTACATGGGAAGCAGTGGTATCAAC65290.22764413597561847No Hit
ACGCAGAGTACATAAGCAGTGGTAT64930.22638893779900301No Hit
ACTCTGCGTTGATACCACTGCTTAT58490.20393483708399332No Hit
GTATCAACGCAGAGTACATAAGCAG58270.20316777153161722No Hit
ATACAGGGTGACAGCCCCGTACACA53130.18524633089882997No Hit
TATCAACGCAGAGTACATAAGCAGT52720.18381679964212905No Hit
GGTATCAACGCAGAGTACATAAGCA49880.17391468069327384No Hit
GGTATCAACGCAGAGTACATGGGGG48590.1694168872270685No Hit
GGATACCACGTGTCCCGCCCTACTC48560.16931228737901718No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45130.15735303808515333No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43470.15156517982631543No Hit
AAGCAGTGGTATCAACGCAGAGTAC41740.14553325525535785No Hit
GTGGTATCAACGCAGAGTACATAAG37490.13071494344809215No Hit
ACCCTGTATCGCGCGCCTTTCCAGA36700.12796048078274158No Hit
ATCATTAACTGAATCCATAGGTTAA34330.11969709278668988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32510.11335136867157844No Hit
ACATAAGCAGTGGTATCAACGCAGA31950.11139883817462108No Hit
GTACTCTGCGTTGATACCACTGCTT31800.11087583893436463No Hit
GTATTTAGCCTTGGAGGATGGTCCC30170.10519258052357802No Hit
GGTATCAACGCAGAGTACATGGGAA29890.10421631527509935No Hit
GAGTACATGTAAGCAGTGGTATCAA28980.10104345321754363No Hit
CCCATATTCAGACAGGATACCACGT28710.10010205458508203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG350.00217205516.2843047
ACGTCGA653.3781635E-614.6138649
AACCGTC801.2910641E-714.2487657
ACTCGGC400.00528357214.2487656
ATGCGCG400.0052866414.247519517
CGAATGG1350.014.06645419
CCGTAGA551.9514558E-413.826182
TACCCTC1051.3606041E-913.5795235
TATGAGT500.001492965913.3083974
CTAGGCC500.001492965913.3083974
TTACCCT655.423395E-513.1621524
TATACCC655.4251777E-513.1616913
GTCGACC655.4645265E-513.15155712
CGAAGCT604.0721518E-412.67466454
CCGCTCG751.4809282E-512.665578
ATCCGTT905.415968E-712.665577
GGTCGAT751.4819943E-512.66468310
CGATGAC751.482261E-512.66446213
GACGCGT1001.447006E-712.34806710
CCGAATG1551.8189894E-1212.25121418
Produced by FastQC (version 0.11.5)