FastQCFastQC Report
Wed 25 May 2016
SRR1295232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868073
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA475101.6565129269722214No Hit
GTATCAACGCAGAGTACTTTTTTTT465971.6246797065486132No Hit
GGTATCAACGCAGAGTACTTTTTTT331971.1574670519195294No Hit
GCTTATGTACTCTGCGTTGATACCA294201.0257758432229584No Hit
TATCAACGCAGAGTACTTTTTTTTT286530.9990331487378459No Hit
CTTATGTACTCTGCGTTGATACCAC208570.7272130102685671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT147270.5134806540837697No Hit
GAGTACATAAGCAGTGGTATCAACG125900.4389706956552361No Hit
GTACTTTTTTTTTTTTTTTTTTTTT124590.43440316895699654No Hit
CCCATGTACTCTGCGTTGATACCAC123020.4289291102423125No Hit
GTGGTATCAACGCAGAGTACATGGG110970.38691483794171205No Hit
TATGTACTCTGCGTTGATACCACTG95580.33325511589140167No Hit
GCGTTGATACCACTGCTTATGTACT95420.3326972500351281No Hit
CATAAGCAGTGGTATCAACGCAGAG92910.32394572941483707No Hit
CATGTACTCTGCGTTGATACCACTG90590.31585667449887084No Hit
GAGTACTTTTTTTTTTTTTTTTTTT77810.2712971392290224No Hit
CTGCTTATGTACTCTGCGTTGATAC74710.26048848826372273No Hit
ACGCAGAGTACATAAGCAGTGGTAT73120.2549446963170045No Hit
GTACTGGTTCACTATCGGTCAGTCA70110.24444984489585864No Hit
ACTCTGCGTTGATACCACTGCTTAT63810.2224838768050883No Hit
GTACATGGGAAGCAGTGGTATCAAC63350.2208800124683019No Hit
GTATCAACGCAGAGTACATAAGCAG60470.2108384270553783No Hit
GCAGAGTACTTTTTTTTTTTTTTTT58820.20508543541255747No Hit
ATACAGGGTGACAGCCCCGTACACA57010.19877457791346315No Hit
TATCAACGCAGAGTACATAAGCAGT55790.19452085075937747No Hit
GGTATCAACGCAGAGTACATAAGCA52640.18353786671399228No Hit
GGTATCAACGCAGAGTACATGGGGG51420.1792841395599066No Hit
GGATACCACGTGTCCCGCCCTACTC51210.1785519406235476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49700.17328708160496614No Hit
AAGCAGTGGTATCAACGCAGAGTAC44420.15487750834793954No Hit
GTGGTATCAACGCAGAGTACTTTTT42560.14839231776875972No Hit
ATCAACGCAGAGTACTTTTTTTTTT41270.14389452430255437No Hit
ACCCTGTATCGCGCGCCTTTCCAGA40850.14243012642983632No Hit
GTGGTATCAACGCAGAGTACATAAG36660.12782101431867318No Hit
ACATAAGCAGTGGTATCAACGCAGA36100.12586848382171584No Hit
ATCATTAACTGAATCCATAGGTTAA35110.12241668883602336No Hit
GCTTACATGTACTCTGCGTTGATAC33530.11690776350532221No Hit
CCCATATTCAGACAGGATACCACGT32880.11464143346421099No Hit
CATGTAAGCAGTGGTATCAACGCAG32520.11338623528759555No Hit
GGTATCAACGCAGAGTACATGGGAA31970.11146857140665527No Hit
GTACTCTGCGTTGATACCACTGCTT30700.10704051117248409No Hit
GCAGTGGTATCAACGCAGAGTACAT29440.10264731755433004No Hit
CCACTGCTTATGTACTCTGCGTTGA29130.10156645245780008No Hit
GAGTACATGTAAGCAGTGGTATCAA28750.10024152104915042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA2400.016.6265132
TTATACG402.7602247E-416.626514
CTTATAC2300.016.5281411
CCGAATG1450.015.72337218
CGAATGG1500.014.56595819
AATCACG851.7200364E-814.52845111
CTTAGAC602.5675534E-514.2512963
TAGTATG602.5713554E-514.249069
TATACAC3150.014.1758913
GCGTCCC707.2726307E-613.57053210
GGGCGTC1205.2750693E-1113.45744511
CGTAATG500.001500833813.29912213
CTATTAG957.357812E-813.0050361
TACATCG1251.0186341E-1012.9213995
TGAACTG1501.8189894E-1212.6680385
GGCGTTC905.4146403E-712.665838
GCGTTCA905.4146403E-712.665839
ATACGTC1001.4453144E-712.3491847
AAACCGT701.091839E-412.21390414
CGTCAAC1251.4133548E-912.15962114
Produced by FastQC (version 0.11.5)