FastQCFastQC Report
Wed 25 May 2016
SRR1295231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6168260
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG73240.11873688852285734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN69200.1121872294617931No Hit
GTATCAACGCAGAGTACTTTTTTTT64470.10451894051158672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.010.1461371
GTGGTAT20300.09.7871561
GCGTCAG2500.09.5061861
TTCGCCG1002.7639177E-49.49790916
TGGTATC20850.08.7983452
GCTAGAC3400.08.6663463
GTATCAA73100.08.3878111
ATTATAC9500.08.3043893
TAGACAG9750.08.2863645
CTAGACT6100.08.2587334
GTCTAGC3700.08.2215651
GTATTAA8450.08.2124441
GTCTAGA6050.08.1706061
TAGACTG8700.08.0846675
AGTACCG1659.688229E-68.0648035
CCAGGAC12000.08.0000623
GTACTAG4050.07.9805011
CTTACTC6100.07.9468233
CTAGTAC3950.07.94090653
GACGGCG1200.0016943987.9155687
Produced by FastQC (version 0.11.5)