##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295231_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6168260
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.22258465110096	31.0	30.0	33.0	26.0	34.0
2	30.22792100203299	31.0	30.0	34.0	26.0	34.0
3	30.429426126654842	31.0	30.0	34.0	26.0	34.0
4	33.710381533852335	35.0	35.0	37.0	28.0	37.0
5	34.01862616037586	37.0	35.0	37.0	28.0	37.0
6	33.91055046317762	36.0	35.0	37.0	28.0	37.0
7	33.91142429145334	37.0	35.0	37.0	28.0	37.0
8	33.959175845376166	37.0	35.0	37.0	28.0	37.0
9	35.07967287371155	39.0	35.0	39.0	27.0	39.0
10	35.08265183374242	39.0	35.0	39.0	27.0	39.0
11	35.496432381255005	39.0	35.0	39.0	28.0	39.0
12	35.48575740970711	39.0	35.0	39.0	28.0	39.0
13	35.417477862476616	39.0	35.0	39.0	27.0	39.0
14	36.090416908496074	39.0	36.0	41.0	27.0	41.0
15	36.438113990006904	40.0	36.0	41.0	27.0	41.0
16	36.41298794149404	40.0	36.0	41.0	27.0	41.0
17	36.09239218191192	39.0	36.0	41.0	27.0	41.0
18	35.9906542525769	39.0	36.0	41.0	27.0	41.0
19	36.409152175816196	40.0	36.0	41.0	27.0	41.0
20	36.42598706928696	40.0	36.0	41.0	27.0	41.0
21	36.46361550907387	40.0	36.0	41.0	27.0	41.0
22	36.37697794840036	40.0	36.0	41.0	27.0	41.0
23	36.102847480488826	39.0	36.0	41.0	27.0	41.0
24	36.38020965393807	40.0	36.0	41.0	27.0	41.0
25	35.10197494917529	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10723879993708962
1101	2	0.15830076411221228
1101	3	-0.05107450857529727
1101	4	0.012052459306673313
1101	5	-0.20280119836218802
1101	6	-0.5188035337972323
1101	7	-0.45391059188510496
1101	8	-0.32350817877101434
1101	9	-0.1480601429936641
1101	10	0.008233895785309642
1101	11	-0.0448973155336887
1101	12	-0.10864358819070219
1101	13	-0.05297030804364056
1101	14	0.3217722826236482
1101	15	0.14412761421277764
1101	16	-0.008852986345367242
1101	17	0.05384021739376976
1101	18	0.2695113817117871
1101	19	-0.003008927317765142
1101	20	0.036071198662412485
1101	21	-0.0999364212244842
1101	22	-0.05679020953263603
1101	23	0.001073622844160127
1101	24	-0.12204596517316446
1101	25	-0.14139353381467146
1102	1	-0.35564095189580414
1102	2	-0.0382249225349085
1102	3	-0.18918116068518742
1102	4	-0.18534189118605582
1102	5	-0.5189307304247208
1102	6	-0.6169084050732678
1102	7	-0.6245015345778313
1102	8	-0.511867396900584
1102	9	-0.4322210282208587
1102	10	-0.04367217788420419
1102	11	-0.2243442697149547
1102	12	-0.3214937067381385
1102	13	-0.40722835930520773
1102	14	-0.20682527537658046
1102	15	-0.2993469408409837
1102	16	-0.4000363538588587
1102	17	-0.5113959676907314
1102	18	-0.1997501491303808
1102	19	-0.34176735093549837
1102	20	-0.24098474095432465
1102	21	-0.2792528455290082
1102	22	-0.23046901779223816
1102	23	-0.3410453094946462
1102	24	-0.35774269988547047
1102	25	-0.439783372709627
1103	1	-0.37413383167654146
1103	2	-0.21014059412544128
1103	3	-0.3779994149044015
1103	4	-0.23301067091220773
1103	5	-0.4257409446489788
1103	6	-0.689719385196355
1103	7	-0.6154326273390112
1103	8	-0.46408575509097005
1103	9	-0.11048009515678103
1103	10	0.0755236443445284
1103	11	-0.14950766889326417
1103	12	-0.2579777579978213
1103	13	-0.25937437879688474
1103	14	-0.23173414061552933
1103	15	-0.18919099894826985
1103	16	-0.2538992803671931
1103	17	-0.09864452928770362
1103	18	0.15790367945524508
1103	19	-0.16933776603049466
1103	20	-0.09448005430945727
1103	21	-0.20819169624422074
1103	22	-0.12829788545847975
1103	23	-2.4891623948781927
1103	24	-1.1606589550995992
1103	25	-0.6195288799107601
1104	1	-0.28451803684588484
1104	2	-0.09759366258100499
1104	3	-0.24392116765451277
1104	4	-0.49071607002895945
1104	5	-0.31404942304934025
1104	6	-0.4288747220732958
1104	7	-0.41654731551243884
1104	8	-0.3190782967234611
1104	9	-2.6285706661305923
1104	10	-0.8795517349888797
1104	11	-0.547882249285017
1104	12	-0.40092321999389924
1104	13	-0.25476268352293374
1104	14	0.023291062477554192
1104	15	-0.16112279220889292
1104	16	-0.14068473941630089
1104	17	0.0902663499988563
1104	18	0.3241497931132358
1104	19	-0.07798275700368862
1104	20	-0.24580140201169343
1104	21	-0.16271458780965986
1104	22	-0.2665441583625423
1104	23	-0.1385454250357654
1104	24	-0.12789946437127497
1104	25	-0.2647805727868686
1105	1	-0.3874261480125689
1105	2	-0.3644761647827366
1105	3	-0.4115392118087762
1105	4	-0.17052283623571185
1105	5	-0.2991448450753751
1105	6	-0.5050360570121697
1105	7	-0.540577794113041
1105	8	-0.6155688655135734
1105	9	-2.432873264900522
1105	10	-0.9276381072575504
1105	11	-0.6585890486536741
1105	12	-0.5556582713133054
1105	13	-0.49214093130579784
1105	14	-0.4596137936577591
1105	15	-0.41410073818216375
1105	16	-0.4913436850670152
1105	17	-1.7825780581426685
1105	18	-0.5169420332017367
1105	19	-0.4729114770265923
1105	20	-0.4792218080004105
1105	21	-0.4030012525554696
1105	22	-0.40913241569531067
1105	23	-0.31550320068730997
1105	24	-0.40175868573696505
1105	25	-0.9575684131494384
1106	1	-0.31966898772795815
1106	2	-0.3731579723114038
1106	3	-0.3235909803960695
1106	4	-0.32042946408119377
1106	5	-0.2706869955044482
1106	6	-0.368189511857004
1106	7	-0.38522959034390425
1106	8	-0.33633278945771394
1106	9	-2.9495390301465463
1106	10	-0.6851071284349288
1106	11	-0.6361147541655612
1106	12	-0.3986048801688611
1106	13	-0.4330362823790921
1106	14	-0.674696514025058
1106	15	-0.5557713861233182
1106	16	-0.5333727979489211
1106	17	-0.40993076601026956
1106	18	-0.09557032639180107
1106	19	-0.32984550505476307
1106	20	-0.3243690099907468
1106	21	-0.26809764446912965
1106	22	-0.30460330281340475
1106	23	-0.05372839359132797
1106	24	-0.3196828341949356
1106	25	-0.5044914889168481
1107	1	-0.25775603119964785
1107	2	-0.08802821283823192
1107	3	-0.17529802733375277
1107	4	-1.4531391639203903
1107	5	-0.44965727861140437
1107	6	-0.4026621995419788
1107	7	-0.22708496561212144
1107	8	-0.1274525131737505
1107	9	0.1633823795534326
1107	10	0.3736680645017145
1107	11	0.14097244389522956
1107	12	-0.5007491045312307
1107	13	-0.10878821483364476
1107	14	0.12136261294435968
1107	15	-0.5793010140050185
1107	16	-0.9338111061810537
1107	17	0.020745900990647215
1107	18	-0.07844763096829155
1107	19	-0.001387904611817703
1107	20	-0.27567891644526554
1107	21	-0.06299253932311188
1107	22	-0.07413466350991627
1107	23	0.07464738453135311
1107	24	-0.1747905233949183
1107	25	-0.312792037917049
1108	1	0.3028680840870521
1108	2	0.3204998383303739
1108	3	0.3959578336195655
1108	4	0.6667793515885734
1108	5	0.5290179878544095
1108	6	0.46830253689996937
1108	7	0.4563072946682354
1108	8	0.40725700212161087
1108	9	0.7839238352954894
1108	10	0.7903696466914809
1108	11	0.48507699827356987
1108	12	0.46297534738841506
1108	13	0.4378753548696892
1108	14	0.47893111583145753
1108	15	0.48215236051493804
1108	16	0.5152410462831511
1108	17	0.5792452728846627
1108	18	0.6619014321505077
1108	19	0.4605962480973531
1108	20	0.42660662506773406
1108	21	0.4298384511226061
1108	22	0.47552579080763024
1108	23	0.6492941589345023
1108	24	0.478004660279403
1108	25	0.5509403929153649
1109	1	0.2902760924131549
1109	2	0.2064586400801005
1109	3	0.34848908328489614
1109	4	0.6465838994018469
1109	5	0.5018722294263256
1109	6	0.41213309502741424
1109	7	0.4375647973626329
1109	8	0.3574318094246749
1109	9	0.8064095260204311
1109	10	0.5521309264114009
1109	11	0.3697890019031007
1109	12	0.4109211303508573
1109	13	0.40841927769939446
1109	14	0.7659437997052763
1109	15	0.4604821694682286
1109	16	0.5415776333397204
1109	17	0.7354147390968535
1109	18	0.49331448949178025
1109	19	0.3330217225499794
1109	20	0.3982092005912037
1109	21	0.36965661939910177
1109	22	0.4607910588211581
1109	23	0.6158690164745835
1109	24	0.3449758645366998
1109	25	0.261966664150485
1110	1	0.27598792963060603
1110	2	0.25992158133030685
1110	3	0.31487452194870613
1110	4	0.5235399692668636
1110	5	0.45929291651253124
1110	6	0.44082144417183855
1110	7	0.33749734161708744
1110	8	0.3636363742841979
1110	9	0.8935478773472241
1110	10	0.814186394665569
1110	11	0.4867517119181102
1110	12	0.3939953369022504
1110	13	0.43638960172378205
1110	14	0.6599480493533534
1110	15	0.45294752801576266
1110	16	0.4591349278947021
1110	17	0.8166904964834742
1110	18	0.8096068310802949
1110	19	0.45397392357321564
1110	20	0.5647530815879591
1110	21	0.44481524642469594
1110	22	0.4974378341893342
1110	23	0.6445640445045342
1110	24	0.43187803889031784
1110	25	0.534251180041899
1111	1	0.16192459860197062
1111	2	0.07762684884164983
1111	3	0.2501200348916939
1111	4	0.5403718993688926
1111	5	0.4463517944994919
1111	6	0.4614013248333464
1111	7	0.3085939700272675
1111	8	0.2931789557752751
1111	9	0.6697836547402218
1111	10	0.5080667819191262
1111	11	0.3234893817338005
1111	12	0.25714068828445846
1111	13	0.2149344416124137
1111	14	0.24359280228875235
1111	15	0.22011829282590156
1111	16	0.12682041840010783
1111	17	0.40405100714965414
1111	18	0.4849441885139285
1111	19	0.2961127479408958
1111	20	-0.2839857764483398
1111	21	-0.37471644798315396
1111	22	-0.06929472883022925
1111	23	0.2139867880672881
1111	24	0.2370264831943416
1111	25	0.1935124128053758
1112	1	0.26867734855543546
1112	2	0.2080628897562491
1112	3	0.275523525710355
1112	4	0.6636216045345265
1112	5	0.5402990904193246
1112	6	0.571870543938374
1112	7	0.474963578839116
1112	8	0.5649609511712725
1112	9	-1.1183952292178745
1112	10	0.2520844107882425
1112	11	0.25131487889541404
1112	12	0.2582719010591461
1112	13	0.24021426111383448
1112	14	0.5849540530618924
1112	15	0.4376772710928165
1112	16	0.35469044892141
1112	17	0.4170640147624667
1112	18	0.5086012845267689
1112	19	0.4342608348196251
1112	20	0.3604385421392564
1112	21	0.38197920477333724
1112	22	0.4026315705433987
1112	23	0.06652371914660904
1112	24	0.2391389450991923
1112	25	0.3283520679762404
1113	1	0.19419608421365453
1113	2	0.17334795118205548
1113	3	0.22639907917926294
1113	4	0.45699337547285523
1113	5	0.4781595468306463
1113	6	0.48280992093124553
1113	7	0.3564886506240583
1113	8	0.3472409474663394
1113	9	0.18510993584246194
1113	10	0.5843502079690381
1113	11	0.3831844704554612
1113	12	0.13709336292247798
1113	13	0.24328228026738685
1113	14	-0.04730334709107353
1113	15	0.12660183015860582
1113	16	0.2764561276441384
1113	17	0.6434735192046901
1113	18	0.35095105064872456
1113	19	0.2891041501197904
1113	20	0.370203783131565
1113	21	0.29956789699489406
1113	22	0.26793900366845946
1113	23	0.08430858970048405
1113	24	0.18466854684761103
1113	25	0.20610977578783007
1114	1	0.4485860237801127
1114	2	0.40672278667913986
1114	3	0.3715433021681456
1114	4	0.6302158340766013
1114	5	0.5777741685268083
1114	6	0.6263768297217709
1114	7	0.393904279112256
1114	8	0.4500390261712397
1114	9	0.11537277505347987
1114	10	0.47766688917769073
1114	11	0.4504632110218907
1114	12	0.48053089092268664
1114	13	0.4610558510508298
1114	14	0.8272561034045225
1114	15	0.49540252220243985
1114	16	0.3688253951501679
1114	17	0.7726074797315121
1114	18	0.6571278782272145
1114	19	0.38275618796825484
1114	20	0.3986097781770681
1114	21	0.49349869966436444
1114	22	0.29198194449406145
1114	23	0.7504773314238946
1114	24	0.5846156918978735
1114	25	0.47028546704009244
1115	1	0.4672122254076463
1115	2	0.43601544701277106
1115	3	0.5527213158824367
1115	4	0.7696940289929088
1115	5	0.705946022051755
1115	6	0.7388510903551548
1115	7	0.6355955144875622
1115	8	0.6115218762402819
1115	9	0.8159208518366441
1115	10	0.5703644482404684
1115	11	0.5579834964725592
1115	12	0.5206152544924194
1115	13	0.47199724550633704
1115	14	0.6137211841821042
1115	15	0.47121010408197606
1115	16	0.5792298345514553
1115	17	0.8108549265878437
1115	18	0.995261762270502
1115	19	0.7167359065797925
1115	20	0.6907010630149202
1115	21	0.5936324954865881
1115	22	0.05381488804667356
1115	23	0.5621190138279815
1115	24	0.6117862272018826
1115	25	0.7429706613841631
1116	1	0.6174314401077474
1116	2	0.5713035842624379
1116	3	0.6866783289882612
1116	4	0.994052741159706
1116	5	0.8666919764207108
1116	6	0.940323199639451
1116	7	0.8723731353135449
1116	8	0.7689836369618916
1116	9	1.1103451519558263
1116	10	0.8933753406546927
1116	11	0.6555580634047615
1116	12	0.6769797200836294
1116	13	0.5976857696959641
1116	14	1.1081976976684444
1116	15	0.7788963732332874
1116	16	0.8809876694974932
1116	17	1.1857022454721644
1116	18	1.209095454099426
1116	19	0.8547088244021097
1116	20	0.7720792606312017
1116	21	0.8168890712489087
1116	22	0.9087536210445322
1116	23	1.2070475303801587
1116	24	0.8476132842230371
1116	25	0.7792571417417093
1201	1	-0.20778340987405386
1201	2	0.03273031641024815
1201	3	-0.15561252497628075
1201	4	-0.041547820656958834
1201	5	-0.2547026725320549
1201	6	-0.44981506594398724
1201	7	-0.41063451591117683
1201	8	-0.35675767564322314
1201	9	0.15533654192297064
1201	10	0.18300293669183532
1201	11	0.031846842623956206
1201	12	0.05459303340705901
1201	13	0.013510152282854904
1201	14	0.41902557557593667
1201	15	0.10358303698184557
1201	16	0.0798136105484275
1201	17	0.1648420358491265
1201	18	0.31001834700632713
1201	19	0.10137428053342745
1201	20	0.13691561388946383
1201	21	-0.05982247341022884
1201	22	0.003700958748616756
1201	23	-0.009578731663062001
1201	24	-0.0731043868672927
1201	25	-9.246710469952291E-4
1202	1	-0.3400772505884291
1202	2	-0.33505511265402177
1202	3	-0.4780709228077811
1202	4	-0.32131656664922303
1202	5	-0.5936171564718435
1202	6	-0.731035684391216
1202	7	-0.6709165447818535
1202	8	-0.5744004599491035
1202	9	0.024204144715007203
1202	10	0.08862870845749882
1202	11	-0.18614412967484384
1202	12	-0.23505495976396418
1202	13	-0.1730441071828892
1202	14	-0.2513186778586345
1202	15	-0.21104644062044287
1202	16	-0.21308349053976627
1202	17	0.13911278111438463
1202	18	0.14492666090512074
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2211	11	0.09042205410269588
2211	12	0.054699366101459646
2211	13	0.08512958648233138
2211	14	0.35276350123910305
2211	15	0.13027325775374266
2211	16	0.27539506617623033
2211	17	0.4282747161636564
2211	18	0.5349504220663377
2211	19	0.29938958374344793
2211	20	0.3702515441883989
2211	21	0.206199375395677
2211	22	0.2079431703990764
2211	23	-1.7927245243178476
2211	24	-0.626191551470427
2211	25	-0.1926174192007295
2212	1	-0.11907443546098406
2212	2	-1.605448351478472
2212	3	-0.5121944541409569
2212	4	0.07085869757720076
2212	5	0.07564683306907938
2212	6	0.1860794052737873
2212	7	0.12068369515292687
2212	8	0.13722425424107598
2212	9	0.5181771658772689
2212	10	0.46361534263763815
2212	11	0.20916563854708414
2212	12	0.054438845531890934
2212	13	-0.002319843788647802
2212	14	0.29382003263017964
2212	15	0.13436631302320023
2212	16	0.16461309843697336
2212	17	0.39593114441996136
2212	18	0.5499458022448209
2212	19	0.2678143061520828
2212	20	0.21173087465383134
2212	21	0.14889810823010663
2212	22	0.19238284090868518
2212	23	0.3985439395725692
2212	24	0.20321995245954128
2212	25	0.09440436448842604
2213	1	0.36423096739942906
2213	2	0.21842317067730832
2213	3	0.2790197303703934
2213	4	0.5491853753743925
2213	5	0.47695660664643924
2213	6	0.5206374255814055
2213	7	0.40794626861806194
2213	8	0.45143360225273454
2213	9	0.6427919353456772
2213	10	0.7184307777576251
2213	11	0.40203393028131273
2213	12	0.4042658612364036
2213	13	0.326219726596797
2213	14	-1.2084637387674704
2213	15	-0.13641738682001403
2213	16	0.19556040219246285
2213	17	0.6146382980149241
2213	18	0.4013636626417636
2213	19	0.30025928689004644
2213	20	0.4480732491176411
2213	21	0.4224581091095203
2213	22	0.39097758019366324
2213	23	0.5944953868638478
2213	24	0.31789576956304444
2213	25	0.22152615259718544
2214	1	0.31348069525315125
2214	2	0.3690349828932966
2214	3	0.3420233773716639
2214	4	0.523959143184463
2214	5	0.4538091813791496
2214	6	0.48697407535402704
2214	7	0.3534199488272236
2214	8	0.2579148048258233
2214	9	0.3296382749113036
2214	10	0.17092009389028107
2214	11	0.17376704089816997
2214	12	0.11194804701803207
2214	13	0.0015150157993488733
2214	14	0.29157584356701705
2214	15	0.06016595537980862
2214	16	0.21653629419439824
2214	17	0.17318257477950993
2214	18	0.48225442521751205
2214	19	0.19472157325262174
2214	20	0.3155298323388678
2214	21	0.322763710720416
2214	22	0.34505239965479717
2214	23	0.3333614609158744
2214	24	0.33545277459899125
2214	25	0.33399649867526904
2215	1	0.45603555008683117
2215	2	0.35983968698265656
2215	3	0.41292995156439005
2215	4	0.7427341639029663
2215	5	0.5870706405526249
2215	6	0.7411697936746506
2215	7	0.5860206864456572
2215	8	0.5616021019674164
2215	9	0.7422161508204681
2215	10	0.7654567552627682
2215	11	0.5425689980871056
2215	12	0.45741931957582693
2215	13	0.38969188392675136
2215	14	0.5127024827961932
2215	15	0.4840468348334497
2215	16	0.5599683955409915
2215	17	0.6578507039624029
2215	18	0.8502786305450769
2215	19	0.514567914503516
2215	20	-0.35156004114190864
2215	21	0.13358624484439474
2215	22	-0.5997895321659072
2215	23	0.28625593445032393
2215	24	0.2261134993771705
2215	25	0.40612136485689376
2216	1	0.19992739173261853
2216	2	-0.22728923265502488
2216	3	0.22123903132532874
2216	4	0.5172105231618076
2216	5	0.4249202966617389
2216	6	0.5939729125921005
2216	7	0.4951941320272084
2216	8	0.44635917478971976
2216	9	0.7887500656898823
2216	10	0.45971964291737066
2216	11	0.3649388605032655
2216	12	-0.07545814454802269
2216	13	0.13882918821484935
2216	14	0.22215946632758232
2216	15	0.20795971914431277
2216	16	0.35244026425099406
2216	17	0.3872644471812876
2216	18	0.7313730210727272
2216	19	0.3798521363523051
2216	20	-0.7136228205988431
2216	21	-0.9805551857397248
2216	22	-0.9647653476528575
2216	23	-1.5275452837142893
2216	24	-1.1750274808413437
2216	25	-0.8739189404333345
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12155.0
3	1616.0
4	1536.0
5	1138.0
6	1207.0
7	1688.0
8	715.0
9	1498.0
10	3360.0
11	7131.0
12	12691.0
13	19324.0
14	26376.0
15	32822.0
16	37472.0
17	40528.0
18	41590.0
19	42162.0
20	42282.0
21	42701.0
22	43509.0
23	45133.0
24	47275.0
25	50670.0
26	54863.0
27	61877.0
28	71933.0
29	86146.0
30	106475.0
31	133801.0
32	175173.0
33	233372.0
34	319938.0
35	450741.0
36	666595.0
37	1087079.0
38	2163688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8809488438999	20.75110990967296	13.501924330502648	23.866016915924497
2	15.78173323901616	21.28470460391258	39.46528275561355	23.46827940145771
3	18.015595970756213	25.97464243576548	30.350864607342636	25.658896986135666
4	13.024158170703767	17.480503327223875	37.659901354841395	31.835437147230962
5	13.571405208470466	37.149744217452835	35.54854238039438	13.730308193682317
6	30.83845260701513	37.922472242383385	17.563237656079153	13.675837494522325
7	25.925959664345623	32.21669767649167	24.175756248786094	17.681586410376617
8	24.233843795015733	36.461837720626924	21.291923711559594	18.012394772797748
9	24.88870994635791	16.838947655242816	21.771221974142804	36.50112042425647
10	15.114495717811499	29.531454652054183	33.9691431180607	21.384906512073623
11	32.287812348635406	25.255975359897135	23.879391879972015	18.57682041149544
12	21.079343349008546	27.029423519848812	31.366831085887476	20.524402045255165
13	26.571799905549547	23.945043107653998	26.79130613524628	22.69185085155017
14	21.117888978755676	23.13868816139981	27.871451208379394	27.87197165146512
15	22.79672076575557	30.59514421208192	24.193081536190462	22.415053485972045
16	22.494741665943003	30.55593771374433	25.51977579185616	21.429544828456507
17	21.05594037642846	29.698123779955854	27.974011656455094	21.271924187160586
18	21.14213293742445	29.593495088656613	28.59458743804707	20.66978453587187
19	21.715739629300007	28.57226284591571	28.756309839877165	20.95568768490712
20	22.553205051695222	28.319797167275528	28.185010060656634	20.94198772037261
21	22.944453997105356	28.55429885195091	27.317250050470044	21.183997100473697
22	22.164645548173514	28.13211578423625	28.450763081986192	21.252475585604046
23	22.39125801462731	28.526553881364574	27.833051567874804	21.24913653613331
24	22.187891998685267	27.80878213667531	28.10046615774161	21.90285970689781
25	22.62495147685192	28.039183448317136	27.675993681704735	21.659871393126213
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16506.0
1	16506.0
2	14028.5
3	11551.0
4	11551.0
5	11551.0
6	12067.5
7	12584.0
8	12584.0
9	12584.0
10	17435.0
11	22286.0
12	22286.0
13	22286.0
14	36297.0
15	50308.0
16	50308.0
17	50308.0
18	81149.5
19	111991.0
20	111991.0
21	111991.0
22	162342.5
23	212694.0
24	212694.0
25	212694.0
26	279474.5
27	346255.0
28	346255.0
29	346255.0
30	419040.5
31	491826.0
32	491826.0
33	491826.0
34	556620.5
35	621415.0
36	621415.0
37	621415.0
38	670784.5
39	720154.0
40	720154.0
41	720154.0
42	729099.5
43	738045.0
44	738045.0
45	738045.0
46	715455.0
47	692865.0
48	692865.0
49	692865.0
50	664784.0
51	636703.0
52	636703.0
53	636703.0
54	583176.0
55	529649.0
56	529649.0
57	529649.0
58	462819.5
59	395990.0
60	395990.0
61	395990.0
62	331633.5
63	267277.0
64	267277.0
65	267277.0
66	210720.0
67	154163.0
68	154163.0
69	154163.0
70	116364.0
71	78565.0
72	78565.0
73	78565.0
74	57038.0
75	35511.0
76	35511.0
77	35511.0
78	25066.5
79	14622.0
80	14622.0
81	14622.0
82	9918.0
83	5214.0
84	5214.0
85	5214.0
86	3423.5
87	1633.0
88	1633.0
89	1633.0
90	998.0
91	363.0
92	363.0
93	363.0
94	216.5
95	70.0
96	70.0
97	70.0
98	45.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14550294572537475
2	0.28181367192692913
3	0.33888000830055803
4	0.366326970652988
5	0.3728928417414311
6	0.33361109940242467
7	0.33633472000207515
8	0.35338977280464834
9	0.3410199959145691
10	0.3715958795511214
11	0.29118422375191705
12	0.33661032446751593
13	0.3080933683080804
14	0.3186149740769682
15	0.3577832322243226
16	0.30760700748671427
17	0.35611339340429876
18	0.32495387678210713
19	0.3391069766838622
20	0.30092765220661905
21	0.3412469642978733
22	0.29505889829546744
23	0.2782470259035773
24	0.31585892942256
25	0.3101847198399549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6168260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.24011870372267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73156845909844	52.939200089200476
2	13.657230328414883	18.36627576893396
3	3.9300015779851227	7.927613178286111
4	1.5065553612276255	4.052038452907013
5	0.7145290181207243	2.4022507997845954
6	0.3765112448999789	1.5189996480216588
7	0.24424955436182888	1.1496358320034514
8	0.15725602715508014	0.8459131146226739
9	0.11509881234875965	0.696533202448929
>10	0.5253256322227554	6.029684521597158
>50	0.02764096032390656	1.275170297569478
>100	0.012968973180941718	1.4624279688228932
>500	5.078490352785037E-4	0.2386922197044621
>1k	4.594709075972843E-4	0.6725327199029736
>5k	9.673071716667474E-5	0.4230321861941065
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	7324	0.11873688852285734	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6920	0.1121872294617931	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6447	0.10451894051158672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.6212027378871837E-5	0.0	4.8636082136615516E-5	0.0
5	0.0	1.6212027378871837E-5	0.0	4.8636082136615516E-5	0.0
6	0.0	1.6212027378871837E-5	0.0	4.8636082136615516E-5	0.0
7	0.0	1.6212027378871837E-5	0.0	8.106013689435918E-5	0.0
8	0.0	1.6212027378871837E-5	0.0	8.106013689435918E-5	0.0
9	0.0	1.6212027378871837E-5	0.0	8.106013689435918E-5	0.0
10	0.0	1.6212027378871837E-5	0.0	8.106013689435918E-5	0.0
11	0.0	1.6212027378871837E-5	0.0	9.727216427323103E-5	1.6212027378871837E-5
12	0.0	1.6212027378871837E-5	0.0	9.727216427323103E-5	1.6212027378871837E-5
13	0.0	1.6212027378871837E-5	0.0	1.4590824640984653E-4	1.6212027378871837E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4085	0.0	10.146137	1
GTGGTAT	2030	0.0	9.787156	1
GCGTCAG	250	0.0	9.506186	1
TTCGCCG	100	2.7639177E-4	9.497909	16
TGGTATC	2085	0.0	8.798345	2
GCTAGAC	340	0.0	8.666346	3
GTATCAA	7310	0.0	8.387811	1
ATTATAC	950	0.0	8.304389	3
TAGACAG	975	0.0	8.286364	5
CTAGACT	610	0.0	8.258733	4
GTCTAGC	370	0.0	8.221565	1
GTATTAA	845	0.0	8.212444	1
GTCTAGA	605	0.0	8.170606	1
TAGACTG	870	0.0	8.084667	5
AGTACCG	165	9.688229E-6	8.064803	5
CCAGGAC	1200	0.0	8.000062	3
GTACTAG	405	0.0	7.980501	1
CTTACTC	610	0.0	7.946823	3
CTAGTAC	395	0.0	7.9409065	3
GACGGCG	120	0.001694398	7.915568	7
>>END_MODULE