FastQCFastQC Report
Wed 25 May 2016
SRR1295231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6168260
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172680.2799492887783589No Hit
GGTATCAACGCAGAGTACTTTTTTT147020.23834922652417376No Hit
TATCAACGCAGAGTACTTTTTTTTT98880.16030452672228473No Hit
GTGGTATCAACGCAGAGTACATGGG78960.12801016818357203No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64750.10497287727819515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG550.00306971312.0911775
AGCGTCA5300.011.6509193
GGTATCA52950.011.4838091
TACGACT700.001492516410.85712054
GTGGTAT21200.010.0388551
TATACCG1356.451173E-79.8520715
TGGTATC20950.09.6586912
GCAGCGT6150.09.4238141
TACCGTA1554.264475E-79.1939767
ACCGTTC1252.7548604E-59.1206468
ATACCGT2151.0440999E-98.8370476
ACCGATC1201.7640699E-48.7089518
GTATCAA96900.08.6284121
GTCTAGA5650.08.4080151
CAGCGTC7350.08.4013432
TAGACCG1252.7450745E-48.3601865
TATACTG11600.08.2717365
GCGTCAG7500.08.2333164
ACCGTAT1752.4406654E-68.1434358
GTCCTAA5500.08.1190861
Produced by FastQC (version 0.11.5)