FastQCFastQC Report
Wed 25 May 2016
SRR1295230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6710463
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC87120.1298271073098831No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN83880.12499882645951553No Hit
GTGGTATCAACGCAGAGTACATGGG77790.11592344671299135No Hit
GAATGGAATGGAATGGAATGGAATG77050.11482069121012961No Hit
GTATCAACGCAGAGTACTTTTTTTT70720.10538766103024486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.011.18477251
GTGGTAT19450.010.7521981
TGGTATC18450.010.302442
CTACGCT951.6380723E-410.0045854
GCGTCAG2900.09.8337131
CGGTCGT1106.852589E-59.49888110
CTGCGCG1002.7621942E-49.4985279
TAGACGT1701.6761805E-78.9452764
ACGTCGC1304.381541E-58.767876
GTCTAAG4750.08.6053611
TATTCCG3600.08.4482545
GTTCTAG7950.08.3699941
GCTACGT1856.0878847E-78.2197383
TATACCG1752.4275978E-68.146535
ACCGTTC1659.775795E-68.0594168
GTATTAG9450.08.047341
GTCTTAT7650.07.95266681
GTATTAT10800.07.92160131
GTATTAA11800.07.89474871
TACGTCG1451.5555762E-47.86561545
Produced by FastQC (version 0.11.5)