FastQCFastQC Report
Wed 25 May 2016
SRR1295230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6710463
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT195080.2907101939165747No Hit
GGTATCAACGCAGAGTACTTTTTTT165870.24718115575631666No Hit
TATCAACGCAGAGTACTTTTTTTTT111610.16632235361405018No Hit
ATTCCATTCCATTCCATTCCATTCC97500.14529548855272728No Hit
GAATGGAATGGAATGGAATGGAATG87380.1302145619460237No Hit
GTGGTATCAACGCAGAGTACATGGG84070.12528196638592598No Hit
ACGCAGAGTACTTTTTTTTTTTTTT75250.11213831295992542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.011.928181
CGGGCGA907.474626E-611.61057418
GTGGTAT21250.011.0004181
AGCGTCA6450.010.6058993
TGGTATC21850.010.1751112
GACTGCG1951.6880222E-99.256127
TCTATAC7900.09.0200483
TAGACAG8250.08.75287155
TCGCGGG1107.1613636E-48.63499815
CAGCGTC8000.08.5510062
GTTCTAG6150.08.4980751
TATACCG1606.4448323E-68.3137885
CGTAGAC1606.448061E-68.31347753
GTATCAA100450.08.2962651
GTACCGT1856.124301E-78.216516
TGTACCG2201.4631951E-88.2058175
GCAGCGT8000.08.1958141
TAGGACT8000.08.1946534
CCCGCGA1050.0045162388.14278419
TACACTG15250.08.0373115
Produced by FastQC (version 0.11.5)