FastQCFastQC Report
Wed 25 May 2016
SRR1295229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286938
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC86020.16270287262683997No Hit
GAATGGAATGGAATGGAATGGAATG74410.14074309174800234No Hit
GTGGTATCAACGCAGAGTACATGGG67230.12716245206582713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN58910.11142555482965755No Hit
GGTATCAACGCAGAGTACATGGGGG57850.1094206135952417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16350.011.6853811
TGGTATC15850.011.3332862
GGTATCA36650.011.2040331
CTAGACG1252.227589E-710.6456024
CTTAGAC2600.09.8702973
TAGGACG1901.0502845E-89.0047394
CTAGGAC4350.08.9584823
TGTACCG2252.6011548E-108.87091355
TCGACCG1401.0880925E-58.82029159
GTATTAG4900.08.7293321
TTAGACT3200.08.6139084
TGCGTTA1554.218382E-68.57970113
GTCCTAT3700.08.4776761
TATACTG9450.08.4484895
GCGTTAC1356.813854E-58.44343614
GTCTAGC2201.452463E-88.20911
GTATCAA63800.08.20911
GTGTAGA6300.08.1473761
CTAGTAC1752.4255114E-68.1469133
GTACCGT2105.832044E-88.1416536
Produced by FastQC (version 0.11.5)