FastQCFastQC Report
Wed 25 May 2016
SRR1295229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286938
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT155940.29495333593849593No Hit
GGTATCAACGCAGAGTACTTTTTTT127640.24142518788758255No Hit
ATTCCATTCCATTCCATTCCATTCC92100.17420291291481005No Hit
TATCAACGCAGAGTACTTTTTTTTT90870.1718764245012898No Hit
GAATGGAATGGAATGGAATGGAATG81710.15455070590954537No Hit
GTGGTATCAACGCAGAGTACATGGG73700.13940015941174266No Hit
GGTATCAACGCAGAGTACATGGGGG63170.11948314884721552No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58450.11055548599208086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45050.012.509031
GTGGTAT17000.011.7390811
TGGTATC17100.011.2785362
AGCGTCA4250.010.5065893
GTACGGA3450.09.0898891
TCGACCG1054.4959472E-49.0476279
GTACTAG3100.08.8899651
ACTTCGT1502.7050828E-68.8666758
CTACACT6750.08.726434
CTAGACT3500.08.6862164
GTATAGA7250.08.6510661
TAACGCA1107.145373E-48.6368634
GTCCTAG4650.08.5834141
GTATCAA82500.08.5700381
TATACTG9900.08.5408975
GACCGAC1356.805033E-58.4443737
TTATACT7550.08.430954
CTAGCAC4300.08.3959133
GTATTAA6050.08.3251
TAGACAG6300.08.2941295
Produced by FastQC (version 0.11.5)