FastQCFastQC Report
Wed 25 May 2016
SRR1295228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG87860.12454048586381854No Hit
GTATCAACGCAGAGTACTTTTTTTT85390.12103929077977994No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN77310.10958598864252005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.011.3245741
TAATACG1804.152753E-99.5051624
TGGTATC22650.09.3574842
GTGGTAT24500.09.3493771
GTATTAG8750.09.2329951
ATCGACC1252.7588825E-59.1195668
TCGACCG1054.498916E-49.0471259
GTCTTAG5600.08.995381
GCGTCGC1651.0539825E-68.63503114
CTAGACA6800.08.526694
AACTGCG1901.01379555E-78.4996567
TAGACAG8650.08.3511185
TTAGTAC4350.08.3029463
GTATAGA10600.08.159571
TCAATAC6550.08.1261453
CTAGTAC3400.08.106943
CTAGACT5900.08.05522254
ACTCGCG1304.1915366E-48.0380798
GCGTCAG2854.9112714E-118.0038351
CGTCAGA2751.9645086E-107.9490932
Produced by FastQC (version 0.11.5)