FastQCFastQC Report
Wed 25 May 2016
SRR1295228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT233640.3311818702165099No Hit
GGTATCAACGCAGAGTACTTTTTTT193010.27358933731590734No Hit
TATCAACGCAGAGTACTTTTTTTTT135620.19223970740782007No Hit
GTGGTATCAACGCAGAGTACATGGG96890.13734040149493942No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90950.12892052343858748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67800.011.7871281
AGCGTCA6750.011.2603263
ACGCGTA803.7706897E-410.6885895
GTGGTAT27650.010.2414841
TGGTCGG2002.5465852E-119.97509710
TCGACCG1151.0275859E-59.9132099
TATTCCG2700.09.5009685
TGGTATC27850.09.4496612
CAGCGTC8500.09.0537352
GCAGCGT7600.09.0024481
TGTACCG2701.8189894E-128.7971935
GTCTTAG5200.08.7716171
GTATTAG9350.08.7403461
GCGTCAG8800.08.6369984
AAGACCG1804.0346094E-78.4453055
GTATCAA119750.08.1813481
ACCGATC1659.754614E-68.0607418
TAGACAG9450.08.0431485
TATACCG1200.0016904637.91747245
TCTAGAC6250.07.90474943
Produced by FastQC (version 0.11.5)