FastQCFastQC Report
Wed 25 May 2016
SRR1295227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6991045
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG92750.13266972248068779No Hit
GTATCAACGCAGAGTACTTTTTTTT81900.11714986815275828No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN80510.11516161031719864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.011.0456061
GTGGTAT23050.010.8470371
TGGTATC22350.010.334652
TTCGCGG1252.7633838E-59.11830114
TAGACAG9300.08.9941595
GTCTAGA6100.08.5715461
CAGTACG1356.819581E-58.4429339
GTTCTAG6200.08.4332961
CTAAGAC5650.08.4117943
TACGCTG1252.7297233E-48.3645685
GGACCGA2855.456968E-128.3322596
GTATCAA88950.08.2828931
TGTACCG2303.6652636E-98.2653835
GTACCGT1502.5932744E-58.2322736
GTAGGAC4650.08.1766253
TCTAGAC6050.08.16986753
GTATTAA9450.08.1485341
ATAAGAC6200.08.1255223
CTAGACT5650.08.0756114
CTACACT10950.08.0733074
Produced by FastQC (version 0.11.5)