FastQCFastQC Report
Wed 25 May 2016
SRR1295227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6991045
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT222470.3182213817819796No Hit
GGTATCAACGCAGAGTACTTTTTTT186320.26651237404422373No Hit
TATCAACGCAGAGTACTTTTTTTTT131190.18765434924249522No Hit
GTGGTATCAACGCAGAGTACATGGG98940.14152390665487063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83260.11909521394870151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66000.011.7052771
GTGGTAT26700.011.6734091
TGGTATC26900.010.7010412
TATACCG1605.456968E-1010.6881035
AGCGTCA6200.010.572953
ACCGTTC1606.186383E-910.0938148
CGCACGA856.5978355E-410.05903212
TCGGCGT1002.757759E-49.50006111
GCAGCGT6900.09.3647231
GGACTGT10200.08.9414266
GTCTTAG6050.08.7956471
CCTATAC5900.08.6952193
TAGACAG10000.08.6454895
GTATCAA118900.08.4634861
CAGCGTC7750.08.4583012
GTCTACG1804.025078E-78.4466131
AGCACCG3050.08.4103115
TAACGCT1701.6132963E-68.3826474
GTCTTAT6250.08.3621471
ACCGTCC1252.7456795E-48.3600548
Produced by FastQC (version 0.11.5)