FastQCFastQC Report
Wed 25 May 2016
SRR1295226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4755683
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63360.13323007441833276No Hit
GTATCAACGCAGAGTACTTTTTTTT58060.12208551326907198No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN53260.11199232581313766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13850.011.8035172
GGTATCA33550.011.4195951
GTGGTAT15700.011.0207191
GCGTCAG2350.010.1137071
TGTACCG1554.0361556E-89.8117975
GCGGTAT950.0018129059.0065221
GTCTAGG4650.08.9957611
CTACGAC1701.674216E-78.9457673
GACCGTC850.0074499928.9396337
CGCCCCC850.0074499928.93963314
TATTCCG1856.52708E-88.73448855
AGACCGT1651.0540771E-68.6347816
TACGACA1554.1809362E-68.5853224
TAGACCG1000.00289237348.5546615
CTAGGAC4800.08.5147863
CTAAGAC4450.08.3301173
CAGGACG1951.5248224E-78.2865654
TGCACCG1502.5708398E-58.2378215
ACCGTGT2909.094947E-128.1884148
ACCGTCT1050.0045215448.1416238
Produced by FastQC (version 0.11.5)