FastQCFastQC Report
Wed 25 May 2016
SRR1295226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4755683
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT159320.33500971364155263No Hit
GGTATCAACGCAGAGTACTTTTTTT139540.29341737033355675No Hit
TATCAACGCAGAGTACTTTTTTTTT93110.1957868091712589No Hit
GTGGTATCAACGCAGAGTACATGGG69740.14664560274517877No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62930.13232589304207198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.012.4314831
CGCACGA701.09176704E-412.2143412
TGGTATC16450.012.0129692
GTGGTAT17650.011.9519521
AGCGTCA4550.011.0666823
GCAGCGT4400.010.7981141
TCGACCG750.002649188710.1333799
CACCGTT951.6455419E-49.9999397
GTCTTAG4700.09.9066951
CAGCGTC5250.09.7720882
AACACCG1201.7010301E-59.5005425
AGGACCG1304.2404827E-69.5005415
ACGCACG800.00451817369.50004211
AGTGCGC900.00111435649.5000428
GATCGTT950.00182482379.0000411
ACACCGT1804.228241E-88.9718856
GACCCGT850.00744175458.940846
TAGACAG5550.08.9014085
CTAACAC5900.08.5344743
GTACTAG2701.4551915E-118.4465251
Produced by FastQC (version 0.11.5)