FastQCFastQC Report
Wed 25 May 2016
SRR1295225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4669247
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63790.13661731752464584No Hit
GGTATCAACGCAGAGTACATGGGGG54890.11755642826348661No Hit
GTATCAACGCAGAGTACTTTTTTTT54650.1170424267553205No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51690.11070307482127205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14850.011.6501581
GGTATCA33150.010.83914851
TTTTACG909.476529E-510.5607072
TGGTATC15550.010.0241822
GTACGGG2201.6370905E-119.5052494
CCGTGCG800.0045241739.4985069
TAGACTG3250.09.3588135
AGTACGG3400.09.2255843
GTCCTAG3900.08.7745491
GACCGAC1304.380757E-58.7678517
TCTATAC5250.08.690423
GTTCATA4650.08.5858491
TAGGACT5100.08.5733614
CGGACCG1000.0028927188.5545415
TAGTACG2901.8189894E-128.5213992
GTCCTAA4800.08.5155771
GTCTTAG4150.08.4750161
GTACTAA3600.08.4495651
TAGCACC3500.08.4189354
GTCTAGG2502.3283064E-108.3650691
Produced by FastQC (version 0.11.5)