##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295225_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4669247
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.200529978388378	31.0	30.0	33.0	26.0	34.0
2	30.4101402217531	31.0	30.0	34.0	26.0	34.0
3	30.366509203732424	31.0	30.0	34.0	26.0	34.0
4	33.54344330038655	35.0	35.0	37.0	27.0	37.0
5	34.02336008354238	35.0	35.0	37.0	28.0	37.0
6	34.1632271756024	37.0	35.0	37.0	30.0	37.0
7	34.09151347101577	37.0	35.0	37.0	29.0	37.0
8	34.08507324628575	37.0	35.0	37.0	29.0	37.0
9	35.10601002688442	39.0	35.0	39.0	27.0	39.0
10	35.11443772411269	39.0	35.0	39.0	27.0	39.0
11	35.56576788505727	39.0	35.0	39.0	29.0	39.0
12	35.53725343722446	39.0	35.0	39.0	28.0	39.0
13	35.488602123640064	39.0	35.0	39.0	28.0	39.0
14	36.156646028792224	39.0	36.0	41.0	27.0	41.0
15	36.514329612462134	40.0	36.0	41.0	27.0	41.0
16	36.48268146876788	40.0	36.0	41.0	27.0	41.0
17	36.14670245544946	39.0	36.0	41.0	27.0	41.0
18	36.0404898263039	39.0	36.0	41.0	27.0	41.0
19	36.452643863132536	40.0	36.0	41.0	27.0	41.0
20	36.4669744393475	40.0	36.0	41.0	27.0	41.0
21	36.5166291267093	40.0	36.0	41.0	27.0	41.0
22	36.43773567772277	40.0	36.0	41.0	27.0	41.0
23	36.13935073471161	39.0	36.0	41.0	27.0	41.0
24	36.401842738240234	39.0	36.0	41.0	27.0	41.0
25	35.10251288912323	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.045649817914991786
1101	2	0.1586190779659944
1101	3	-0.0452753845336602
1101	4	-0.007037027566362042
1101	5	-0.07390045497033526
1101	6	-0.16664544193830721
1101	7	-0.12166261667449163
1101	8	-0.04020199836693905
1101	9	0.1358071971582575
1101	10	0.1845876584947277
1101	11	0.19057722262584065
1101	12	0.05891474513761352
1101	13	0.10843653869999059
1101	14	0.5202793772959424
1101	15	0.2924416201583
1101	16	0.17738708580605334
1101	17	0.2566766933118245
1101	18	0.45827215423447853
1101	19	0.1855661410342222
1101	20	0.19936324933889438
1101	21	0.14248787706453214
1101	22	0.2047408169490339
1101	23	0.22893054188284623
1101	24	0.15733036997590943
1101	25	0.06179764996560522
1102	1	-0.23859148641924577
1102	2	-0.0033756548616423743
1102	3	-0.17088178441906265
1102	4	-0.21028241533919356
1102	5	-0.43394477100628137
1102	6	-0.44453563353581416
1102	7	-0.4254801120806917
1102	8	-0.41110146395079283
1102	9	-0.4020906748922215
1102	10	0.05384293100643589
1102	11	-0.16278973643863992
1102	12	-0.20781100798229346
1102	13	-0.2202018460894024
1102	14	-0.09679755425032965
1102	15	-0.16369768308355503
1102	16	-0.2631998298335887
1102	17	-0.3354643607561911
1102	18	0.016696375149130915
1102	19	-0.23612522346918752
1102	20	-0.18062979259990897
1102	21	-0.1422492661302286
1102	22	-0.23262525310198612
1102	23	-0.16291514142818642
1102	24	-0.2353974753464101
1102	25	-0.22626321301099495
1103	1	-0.45383760507693793
1103	2	-0.22619851773700717
1103	3	-0.5194931940983167
1103	4	-0.39377911993143044
1103	5	-0.46852482030794107
1103	6	-0.5323498688814325
1103	7	-0.5072372409726924
1103	8	-0.3821475368948697
1103	9	-0.03517425706139932
1103	10	0.07804902305189643
1103	11	-0.05064011378518529
1103	12	-0.2612904470542574
1103	13	-0.13310197752912956
1103	14	-0.26755977388176433
1103	15	-0.2506311206062719
1103	16	-0.21510418899825368
1103	17	-0.08363233789729208
1103	18	0.16780561029338514
1103	19	-0.07077410471472945
1103	20	-0.11593519250774875
1103	21	-0.16617840897900038
1103	22	-0.12381152144300245
1103	23	-2.4518027575886236
1103	24	-1.1275058092943127
1103	25	-0.6743875163883075
1104	1	-0.32067689688682677
1104	2	-0.028049567432926636
1104	3	-0.22773807305921423
1104	4	-0.4940267147758419
1104	5	-0.2856679275658607
1104	6	-0.3764408605872589
1104	7	-0.2949414626823028
1104	8	-0.3183067662415695
1104	9	-2.6205897811006764
1104	10	-0.7583651139544969
1104	11	-0.4645496756114369
1104	12	-0.25269602086071075
1104	13	-0.1672127912346255
1104	14	0.0051840024186802225
1104	15	-0.13283202179065512
1104	16	-0.1776075418173022
1104	17	0.2069376800404541
1104	18	0.3410852237924118
1104	19	-0.09318633558511635
1104	20	-0.11024833099458675
1104	21	-0.1253470646513577
1104	22	-0.0928044373533723
1104	23	0.026983112237388696
1104	24	-0.05614223346642433
1104	25	-0.17440878760648815
1105	1	-0.16118592297863898
1105	2	-0.14472517413987873
1105	3	-0.2677505228661303
1105	4	-0.10109252439258398
1105	5	-0.094034542164799
1105	6	-0.1374162888719752
1105	7	-0.20663093664392562
1105	8	-0.35097671655300644
1105	9	-2.2481968985084464
1105	10	-0.674573312350006
1105	11	-0.38243959168280384
1105	12	-0.29046536141480317
1105	13	-0.12121298969186967
1105	14	-0.1298412189783562
1105	15	-0.1467990874976195
1105	16	-0.26332531056961983
1105	17	-1.4263484741959118
1105	18	-0.2647071942962853
1105	19	-0.2335688834034073
1105	20	-0.22456723970396553
1105	21	-0.21327681995925474
1105	22	-0.11784590516238325
1105	23	-0.10523755372863519
1105	24	-0.09048787711874695
1105	25	-0.4945803686744483
1106	1	-0.38148374540236674
1106	2	-0.4383089737420356
1106	3	-0.4527258439787225
1106	4	-0.4968983160565372
1106	5	-0.36998814337383834
1106	6	-0.48831934529903265
1106	7	-0.49149876766375655
1106	8	-0.5155491301962911
1106	9	-3.209587008023437
1106	10	-0.9302129928356067
1106	11	-0.7897195143489029
1106	12	-0.49527793841510714
1106	13	-0.5579898463107114
1106	14	-0.7034295338626535
1106	15	-0.6687515823849566
1106	16	-0.6921972813739004
1106	17	-0.5140893668252957
1106	18	-0.17836941395312778
1106	19	-0.30593822727550446
1106	20	-0.4429923061918828
1106	21	-0.3440206545044191
1106	22	-0.3542130891469384
1106	23	-0.25773511764857915
1106	24	-0.4127809965115006
1106	25	-0.7522926179676759
1107	1	-0.40621024657130533
1107	2	-0.06194508102821317
1107	3	-0.21352124148974028
1107	4	-1.5113157675692577
1107	5	-0.40915236374221564
1107	6	-0.302406000579019
1107	7	-0.14927922225762558
1107	8	-0.1834088318283591
1107	9	0.13311804234693625
1107	10	0.3557511551974315
1107	11	-0.010879285787630977
1107	12	-0.5536217120974598
1107	13	-0.20136691033898302
1107	14	0.04922024587471441
1107	15	-0.714149936296316
1107	16	-0.9791501400372411
1107	17	-0.15714862279813246
1107	18	-0.21518193133774588
1107	19	-0.11500037359670756
1107	20	-0.4531394442335994
1107	21	-0.16406417064538914
1107	22	-0.1292697735372954
1107	23	0.06674533610240019
1107	24	-0.19894084840796467
1107	25	-0.3398193318515368
1108	1	0.33394497750595775
1108	2	0.36735163268245685
1108	3	0.37104026696277614
1108	4	0.7636598107797639
1108	5	0.49425284041812034
1108	6	0.4947775692962395
1108	7	0.373877041175696
1108	8	0.42469994449194104
1108	9	0.7492505477676303
1108	10	0.8238344661279058
1108	11	0.552818261387209
1108	12	0.5126737480662484
1108	13	0.42684221548007883
1108	14	0.5429778887964289
1108	15	0.5249403242453639
1108	16	0.6293235737552152
1108	17	0.7080417607513212
1108	18	0.8364625175586013
1108	19	0.603984977936868
1108	20	0.579574840864673
1108	21	0.4173351767014921
1108	22	0.5466837594780429
1108	23	0.7196601809387602
1108	24	0.5001134209928679
1108	25	0.6420815544534548
1109	1	0.3927762507405639
1109	2	0.31116862919968113
1109	3	0.40030391441430524
1109	4	0.7280840817853331
1109	5	0.5725495876271438
1109	6	0.45311972955207125
1109	7	0.4276883644834655
1109	8	0.38270008745386974
1109	9	0.8470026275671927
1109	10	0.6546834996277013
1109	11	0.4700895188770531
1109	12	0.47617750425587246
1109	13	0.49852842969340827
1109	14	0.8839324779603643
1109	15	0.5292287435183809
1109	16	0.53181206231951
1109	17	0.8563818004402819
1109	18	0.5923913758559962
1109	19	0.4395791088057024
1109	20	0.46502636597966784
1109	21	0.39786084217541884
1109	22	0.44916656325275284
1109	23	0.6465337147587107
1109	24	0.4743461296789562
1109	25	0.4757621219863495
1110	1	0.30767245309058566
1110	2	0.24749833189172676
1110	3	0.4116118534805828
1110	4	0.5737521966927019
1110	5	0.4992319464461872
1110	6	0.4196577663962131
1110	7	0.3872135941046366
1110	8	0.4334229299395247
1110	9	1.072964369746245
1110	10	0.9491728845590188
1110	11	0.5737106017727598
1110	12	0.49104994043359085
1110	13	0.5027998025099762
1110	14	0.6941326532984107
1110	15	0.6053677780558644
1110	16	0.6011053173783409
1110	17	0.9436452400285091
1110	18	1.0941964545140905
1110	19	0.5986363963565964
1110	20	0.6482134282642562
1110	21	0.569308980221578
1110	22	0.6297606761160637
1110	23	0.7277525006674423
1110	24	0.6390156923506538
1110	25	0.5602550804182016
1111	1	0.13596631585551222
1111	2	0.10972193128110774
1111	3	0.2255860651483239
1111	4	0.5282270451723363
1111	5	0.3347122241734368
1111	6	0.2636153705537083
1111	7	0.20047151803318286
1111	8	0.10895810722220034
1111	9	0.5347263988427002
1111	10	0.4229561612103012
1111	11	0.23054788402693305
1111	12	0.08741617084746878
1111	13	0.11969512534255955
1111	14	0.09451513150706603
1111	15	0.09398617494045425
1111	16	-0.0046288660413935645
1111	17	0.37467204497181683
1111	18	0.4786338004074224
1111	19	0.3256766751700795
1111	20	-0.20994813106030819
1111	21	-0.32199163245333295
1111	22	-0.21181049617884184
1111	23	0.10524224291021511
1111	24	0.04238057077965607
1111	25	0.022019814142311134
1112	1	0.07819773199933877
1112	2	0.07776564455852153
1112	3	0.2346285109685624
1112	4	0.6368422634176554
1112	5	0.461466338469819
1112	6	0.37025366514711067
1112	7	0.3146893645809712
1112	8	0.42241320732958343
1112	9	-1.2067681534623844
1112	10	0.17321309377953753
1112	11	0.11664089213517315
1112	12	0.15706918263064296
1112	13	0.14658685209736433
1112	14	0.4704082212146474
1112	15	0.3137309448273271
1112	16	0.2946380570145024
1112	17	0.37717221867502815
1112	18	0.47946115456340976
1112	19	0.3470145093100214
1112	20	0.24720364998210442
1112	21	0.29510441867171977
1112	22	0.2870707234251526
1112	23	0.12294926832423414
1112	24	0.17698508232128773
1112	25	0.2572090222170189
1113	1	0.27760653746714326
1113	2	0.12869814508619726
1113	3	0.31135894370958184
1113	4	0.5431999667382499
1113	5	0.5629993282356907
1113	6	0.4369607454882072
1113	7	0.2711046950787548
1113	8	0.3025373714281301
1113	9	0.21952581598598897
1113	10	0.4948465512667255
1113	11	0.28601575802061063
1113	12	0.07172244764861802
1113	13	0.10345546418258778
1113	14	-0.24726201022792793
1113	15	-0.005495586332770586
1113	16	0.13227055780565422
1113	17	0.6104631183820786
1113	18	0.3636333460911487
1113	19	0.21873739357878463
1113	20	0.34054880881618743
1113	21	0.2355680942288103
1113	22	0.20756025875718365
1113	23	-0.08147714146311102
1113	24	0.019044937007706153
1113	25	0.07276875526721227
1114	1	0.4787241553491519
1114	2	0.39803808058132617
1114	3	0.4761937384924195
1114	4	0.7491631342330507
1114	5	0.6551289186631948
1114	6	0.5782111534274321
1114	7	0.3253206471825507
1114	8	0.4063151146857251
1114	9	0.24000696429687451
1114	10	0.543213340387986
1114	11	0.43461532487427945
1114	12	0.4172675780068573
1114	13	0.44555880034952366
1114	14	0.8324455481966595
1114	15	0.501618677934708
1114	16	0.398926644921886
1114	17	0.8507468572539665
1114	18	0.7088584532100342
1114	19	0.5121201296207829
1114	20	0.5132876427322657
1114	21	0.49820547143646365
1114	22	0.33125327119138603
1114	23	0.7389060863660148
1114	24	0.5446197132099115
1114	25	0.5451317200806258
1115	1	0.43557761331265255
1115	2	0.31495114164561144
1115	3	0.5061463161303763
1115	4	0.7660794999242242
1115	5	0.5508959428544173
1115	6	0.44628347180485406
1115	7	0.43501193898825363
1115	8	0.4634051530135963
1115	9	0.6518679465672577
1115	10	0.42172402815040755
1115	11	0.3432295595449162
1115	12	0.37234463475542157
1115	13	0.37188355472974877
1115	14	0.34236402826927304
1115	15	0.35800531386898626
1115	16	0.38728991347229424
1115	17	0.6502844220686157
1115	18	0.8328741430373725
1115	19	0.5330865184733327
1115	20	0.5505066310796849
1115	21	0.3485837739134041
1115	22	-0.13795249555645483
1115	23	0.31528385971615336
1115	24	0.43178002027352136
1115	25	0.5149672598160606
1116	1	0.6286187605721771
1116	2	0.48158032027335906
1116	3	0.700379339573999
1116	4	1.0576919934265803
1116	5	0.8294658131602546
1116	6	0.6592992009947594
1116	7	0.5608458386363324
1116	8	0.49338455266100567
1116	9	0.9075363910152561
1116	10	0.6536828190765149
1116	11	0.4117957913617474
1116	12	0.4515884528903129
1116	13	0.4770254833799896
1116	14	0.9624378365309809
1116	15	0.5940068027394503
1116	16	0.7129056749578311
1116	17	1.0511560427482465
1116	18	1.017029807221384
1116	19	0.6592465887857557
1116	20	0.6654678582528248
1116	21	0.7161232024481023
1116	22	0.7259391432794828
1116	23	0.9638667034321102
1116	24	0.7241121471805201
1116	25	0.6748670786904398
1201	1	-0.23903417808190497
1201	2	0.10969249881298637
1201	3	-0.22860408791025577
1201	4	-0.2668291152904345
1201	5	-0.22608335617201902
1201	6	-0.3096918970598139
1201	7	-0.2960793457814077
1201	8	-0.19224184617491602
1201	9	0.1673268708076563
1201	10	0.246762863785996
1201	11	0.15829528576107776
1201	12	0.07652906367722778
1201	13	0.04853739362721399
1201	14	0.4321132844026181
1201	15	0.13979082114172314
1201	16	0.09725652187224654
1201	17	0.17746254772922754
1201	18	0.3849055297775621
1201	19	0.0720045760778163
1201	20	0.1344652064017282
1201	21	-0.015239007386391279
1201	22	-0.03706658390212425
1201	23	-0.12003276948252051
1201	24	-0.198991077921427
1201	25	-0.05553084102248107
1202	1	-0.26195204107887804
1202	2	-0.19470174030809773
1202	3	-0.38870806215985
1202	4	-0.19259242575382984
1202	5	-0.38179428138787586
1202	6	-0.40183479487112095
1202	7	-0.2519598284774034
1202	8	-0.257825453449513
1202	9	0.2830243882768926
1202	10	0.37160042328500964
1202	11	0.07326686707465058
1202	12	0.006898988200859435
1202	13	0.029389059514045357
1202	14	3.3408803972179157E-6
1202	15	0.06859738186207665
1202	16	0.0639677411852233
1202	17	0.3580852716336764
1202	18	0.3697804785674208
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2211	9	-1.5842090588560822
2211	10	-0.05464649400551025
2211	11	-0.1403450843911358
2211	12	-0.1631538004284394
2211	13	-0.09995544125476385
2211	14	0.15379455417059518
2211	15	0.021416870678130806
2211	16	0.14028747764839977
2211	17	0.2520444057754574
2211	18	0.37048514324468584
2211	19	0.05555846237434281
2211	20	0.14790712611374346
2211	21	0.040178509251177275
2211	22	0.07782249243586392
2211	23	-2.0575919985157327
2211	24	-0.6836090075300589
2211	25	-0.42961082814883866
2212	1	0.05214955808441957
2212	2	-1.561703122028888
2212	3	-0.30472256257304053
2212	4	0.35038318180954064
2212	5	0.2927800350669756
2212	6	0.2979968044624286
2212	7	0.23753334908479928
2212	8	0.2675560875396812
2212	9	0.7650606094242391
2212	10	0.5780583665383148
2212	11	0.349257787230151
2212	12	0.21124521722747858
2212	13	0.1403648414919516
2212	14	0.44197835526286156
2212	15	0.2915105529400748
2212	16	0.37327539018135525
2212	17	0.6752067899416332
2212	18	0.7835689276951427
2212	19	0.4271785424255512
2212	20	0.36453551804454065
2212	21	0.34299312728514764
2212	22	0.28335940586228503
2212	23	0.5789773511787288
2212	24	0.31492025386253175
2212	25	0.26316187328968965
2213	1	0.16531526519303696
2213	2	0.04017570943971904
2213	3	0.15115692446847007
2213	4	0.35342739662937106
2213	5	0.2732358686406329
2213	6	0.23326081581112845
2213	7	0.1580774835472596
2213	8	0.19248607813764096
2213	9	0.4342669749457997
2213	10	0.485480961348145
2213	11	0.11103418028451273
2213	12	0.1622219196778758
2213	13	-0.01731804405309134
2213	14	-1.5138056788598746
2213	15	-0.4831526914724904
2213	16	-0.07466709847258812
2213	17	0.3722578310642817
2213	18	0.03890735668568368
2213	19	-0.02605929560926512
2213	20	0.04818405969925266
2213	21	0.10508475402905049
2213	22	0.10755321548846553
2213	23	0.36487841518196973
2213	24	0.051824218162551006
2213	25	-0.13153898105915118
2214	1	0.3242127197676119
2214	2	0.2657679437417606
2214	3	0.3416341902700957
2214	4	0.5976362428825226
2214	5	0.3314741843676785
2214	6	0.22227697201321206
2214	7	0.1590752146320682
2214	8	-0.03121274512695038
2214	9	0.23150623750461818
2214	10	0.08703456267723197
2214	11	-0.01541968987120157
2214	12	-0.09046416874894447
2214	13	-0.16621410890697064
2214	14	0.046176226814253596
2214	15	-0.0954263872701091
2214	16	0.09053075996871485
2214	17	0.04012181591568975
2214	18	0.2929381284027315
2214	19	0.011693869612649621
2214	20	0.18694549840377306
2214	21	0.1755774303091684
2214	22	0.22801326805255684
2214	23	0.16520403007926632
2214	24	0.14397803183663171
2214	25	0.09576979729518342
2215	1	0.29658754356174555
2215	2	0.2250353266813896
2215	3	0.3137920041688176
2215	4	0.6886222465177667
2215	5	0.4461257071110438
2215	6	0.47268444568722856
2215	7	0.3967019675054857
2215	8	0.36424119976631886
2215	9	0.5269702468074087
2215	10	0.5355126917439748
2215	11	0.3122942851865105
2215	12	0.13768924954815276
2215	13	0.0688818272211762
2215	14	0.3116909458108097
2215	15	0.15981725553526616
2215	16	0.23823826466341558
2215	17	0.4247499638655725
2215	18	0.6771986151082245
2215	19	0.23837889145979574
2215	20	-0.662108721421454
2215	21	-0.18627993263385179
2215	22	-0.9703358629859693
2215	23	0.0874841234057584
2215	24	0.026080786489799834
2215	25	0.23921177318611342
2216	1	0.12177979294847674
2216	2	-0.348476980035624
2216	3	0.1383209127803049
2216	4	0.4915346983979987
2216	5	0.30334390212588147
2216	6	0.261947880175434
2216	7	0.15427755803496268
2216	8	0.21153317077042288
2216	9	0.5340299566647246
2216	10	0.32486065164952294
2216	11	0.15180808028587478
2216	12	-0.296583969675865
2216	13	-0.09583983609203273
2216	14	-0.021517436905583054
2216	15	0.04775676473659729
2216	16	0.11837387283297574
2216	17	0.12037026099078929
2216	18	0.34644881953110485
2216	19	0.09239430816091954
2216	20	-0.9769530384652754
2216	21	-1.241871932119352
2216	22	-1.303487282921104
2216	23	-1.7719390824963241
2216	24	-1.5267209554688037
2216	25	-1.2090485650993088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9442.0
3	1269.0
4	1076.0
5	845.0
6	1024.0
7	1217.0
8	623.0
9	1226.0
10	2767.0
11	5353.0
12	9518.0
13	14484.0
14	19354.0
15	23815.0
16	26903.0
17	29042.0
18	29369.0
19	30223.0
20	30129.0
21	30473.0
22	31679.0
23	32881.0
24	34707.0
25	37059.0
26	41054.0
27	46232.0
28	53921.0
29	65757.0
30	81508.0
31	102500.0
32	133290.0
33	178903.0
34	245250.0
35	346072.0
36	508941.0
37	824739.0
38	1636602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97833525429385	19.485488562385175	13.101343928856831	24.43483225446414
2	16.269322970329654	20.449942684307484	38.98139371746124	24.29934062790163
3	18.631161138484757	25.25702773872971	29.60527149982044	26.506539622965093
4	14.281995290419136	16.97196823419094	35.92708533524915	32.818951140140776
5	14.010461231527305	37.19094649851875	34.8801666573506	13.918425612603356
6	31.939920866395212	37.381645576692804	16.81569761094908	13.862735945962907
7	27.346691154190168	32.26153139287045	22.058047981047242	18.333729471892145
8	25.452938871549158	36.0657580555961	19.962626135181708	18.51867693767304
9	25.81350701789564	16.871984359448987	20.37748281604708	36.93702580660829
10	15.869947831313723	29.334249999086342	32.88757666380531	21.908225505794622
11	33.51676665318429	24.367683458201025	23.265257726356207	18.850292162258476
12	22.14929609609429	26.30270414545664	29.965187869243582	21.582811889205487
13	27.466911871336297	22.97451024413405	26.05343299069848	23.505144893831176
14	21.977814433574476	22.51576940538754	26.642167874524585	28.864248286513394
15	23.34435441956162	30.154822545608685	23.414384387573097	23.086438647256603
16	23.1948204735324	29.53158078594364	24.98220053391953	22.29139820660443
17	22.31544460866373	29.07369146893719	26.530055138129054	22.08080878427003
18	22.316743042086777	28.767174799936225	27.333712094677733	21.582370063299273
19	22.895579117757737	28.229074636142066	27.485788570724896	21.3895576753753
20	23.333979240181534	27.655933871741805	27.233867402341676	21.776219485734984
21	23.594152627427718	27.54703924109666	26.875971141385293	21.98283699009033
22	23.569366542771053	27.0074537142155	27.127618783299678	22.29556095971377
23	23.65286996637719	27.284928367623735	26.85117153647984	22.21103012951924
24	23.368846966086405	27.185020891572876	26.96161309112129	22.48451905121943
25	23.392308017882975	27.209874866875655	26.930380882259104	22.467436232982266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14105.0
1	14105.0
2	12165.5
3	10226.0
4	10226.0
5	10226.0
6	9958.5
7	9691.0
8	9691.0
9	9691.0
10	11956.0
11	14221.0
12	14221.0
13	14221.0
14	21052.0
15	27883.0
16	27883.0
17	27883.0
18	44609.0
19	61335.0
20	61335.0
21	61335.0
22	91300.0
23	121265.0
24	121265.0
25	121265.0
26	163767.0
27	206269.0
28	206269.0
29	206269.0
30	258563.5
31	310858.0
32	310858.0
33	310858.0
34	365506.0
35	420154.0
36	420154.0
37	420154.0
38	462221.0
39	504288.0
40	504288.0
41	504288.0
42	531542.0
43	558796.0
44	558796.0
45	558796.0
46	561897.5
47	564999.0
48	564999.0
49	564999.0
50	550603.5
51	536208.0
52	536208.0
53	536208.0
54	499022.0
55	461836.0
56	461836.0
57	461836.0
58	402496.5
59	343157.0
60	343157.0
61	343157.0
62	290163.0
63	237169.0
64	237169.0
65	237169.0
66	189171.0
67	141173.0
68	141173.0
69	141173.0
70	107067.0
71	72961.0
72	72961.0
73	72961.0
74	52972.0
75	32983.0
76	32983.0
77	32983.0
78	23083.5
79	13184.0
80	13184.0
81	13184.0
82	8993.5
83	4803.0
84	4803.0
85	4803.0
86	3064.0
87	1325.0
88	1325.0
89	1325.0
90	810.0
91	295.0
92	295.0
93	295.0
94	176.0
95	57.0
96	57.0
97	57.0
98	31.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14239983449151436
2	0.28814067878610833
3	0.345880181536766
4	0.3737861800842834
5	0.3809393677395948
6	0.33947657941419673
7	0.34251775500417947
8	0.3597582222572505
9	0.3472508522252089
10	0.37706293969884225
11	0.29525103298240596
12	0.3423250044386172
13	0.31336958614526067
14	0.32424928473477627
15	0.3630778153308231
16	0.3129626682846292
17	0.36260664728167086
18	0.3315523894966362
19	0.3453661800285999
20	0.3061093148424146
21	0.34731510241372965
22	0.29949154542477624
23	0.282315328360226
24	0.3207797745546552
25	0.3161965944401742
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4669247.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.22318206878824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00603051669343	44.25312953099317
2	13.128246882564964	15.287366169751628
3	4.581012264641991	8.001633334308076
4	2.098597936124251	4.887481988965819
5	1.1457908632549552	3.3355795022023647
6	0.7226668314713188	2.5245577502297336
7	0.45558974422120757	1.8568119238524823
8	0.3309068365184618	1.5413159192336914
9	0.2540962607341437	1.3314863566550101
>10	1.2061934012953053	12.059699395363118
>50	0.053726632286206034	2.132974840616381
>100	0.016144910628325668	1.501733934381331
>500	4.0615266412216927E-4	0.17445401430726612
>1k	4.4307517587024634E-4	0.6280339693254667
>5k	1.4769172529006052E-4	0.4837413698144946
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6379	0.13661731752464584	No Hit
GGTATCAACGCAGAGTACATGGGGG	5489	0.11755642826348661	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5465	0.1170424267553205	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5169	0.11070307482127205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1416729506920495E-5	0.0	0.0	0.0
3	0.0	4.283345901384099E-5	0.0	2.1416729506920495E-5	0.0
4	0.0	4.283345901384099E-5	0.0	2.1416729506920495E-5	0.0
5	0.0	8.566691802768198E-5	0.0	2.1416729506920495E-5	0.0
6	0.0	8.566691802768198E-5	0.0	2.1416729506920495E-5	0.0
7	0.0	1.2850037704152298E-4	0.0	4.283345901384099E-5	0.0
8	0.0	1.7133383605536396E-4	0.0	4.283345901384099E-5	0.0
9	0.0	1.7133383605536396E-4	0.0	4.283345901384099E-5	0.0
10	0.0	1.9275056556228445E-4	0.0	4.283345901384099E-5	0.0
11	0.0	1.9275056556228445E-4	0.0	4.283345901384099E-5	0.0
12	0.0	1.9275056556228445E-4	0.0	4.283345901384099E-5	0.0
13	0.0	2.1416729506920496E-4	0.0	4.283345901384099E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	1485	0.0	11.650158	1
GGTATCA	3315	0.0	10.8391485	1
TTTTACG	90	9.476529E-5	10.560707	2
TGGTATC	1555	0.0	10.024182	2
GTACGGG	220	1.6370905E-11	9.505249	4
CCGTGCG	80	0.004524173	9.498506	9
TAGACTG	325	0.0	9.358813	5
AGTACGG	340	0.0	9.225584	3
GTCCTAG	390	0.0	8.774549	1
GACCGAC	130	4.380757E-5	8.767851	7
TCTATAC	525	0.0	8.69042	3
GTTCATA	465	0.0	8.585849	1
TAGGACT	510	0.0	8.573361	4
CGGACCG	100	0.002892718	8.554541	5
TAGTACG	290	1.8189894E-12	8.521399	2
GTCCTAA	480	0.0	8.515577	1
GTCTTAG	415	0.0	8.475016	1
GTACTAA	360	0.0	8.449565	1
TAGCACC	350	0.0	8.418935	4
GTCTAGG	250	2.3283064E-10	8.365069	1
>>END_MODULE