FastQCFastQC Report
Wed 25 May 2016
SRR1295225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4669247
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150380.3220647783250704No Hit
GGTATCAACGCAGAGTACTTTTTTT124720.2671094504103124No Hit
TATCAACGCAGAGTACTTTTTTTTT88500.18953805613624639No Hit
GTGGTATCAACGCAGAGTACATGGG69560.14897477045013896No Hit
GGTATCAACGCAGAGTACATGGGGG62250.13331914118058008No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56180.12031918636987934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG500.001500135413.3002329
GTGGTAT16500.012.3817881
GGTATCA44050.012.0369691
TGGTATC16850.011.6143232
TCGACCG1303.8461076E-710.2309489
ACACCGT2500.09.8797496
GTACCGT1651.0244548E-89.7876316
GTGCTAG2700.09.5023031
ACCGTTC1401.0596959E-69.5001658
AGCGTCA4700.09.5000653
TTCGCGG800.0045188599.4998614
AACACCG1856.704795E-99.2436035
ACACCGC1451.7089715E-69.1721816
TAGACAG5500.09.15490155
TGTACCG2304.1836756E-119.087315
GTTCTAT4100.09.0387761
GCGGTAT1401.0829044E-58.8235671
CGGTATC1201.7650543E-48.7083922
TACACTC4950.08.63675
GTCCGTT1107.1470847E-48.63660711
Produced by FastQC (version 0.11.5)