FastQCFastQC Report
Wed 25 May 2016
SRR1295224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944439
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA408012.098343018217594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA379431.9513597495215844No Hit
GCTTATGTACTCTGCGTTGATACCA239441.2314091622313685No Hit
CTTATGTACTCTGCGTTGATACCAC155060.7974536614416806No Hit
GTATCAACGCAGAGTACTTTTTTTT101870.5239043240749646No Hit
GAGTACATAAGCAGTGGTATCAACG96580.49669853361303695No Hit
TATGTACTCTGCGTTGATACCACTG82790.42577833503648094No Hit
CCCATGTACTCTGCGTTGATACCAC79170.40716114005119214No Hit
GCGTTGATACCACTGCTTATGTACT73020.3755324800623727No Hit
GGTATCAACGCAGAGTACTTTTTTT71270.3665324548623022No Hit
TATCAACGCAGAGTACTTTTTTTTT67280.3460123974061413No Hit
CATAAGCAGTGGTATCAACGCAGAG65300.33582951175120435No Hit
GTGGTATCAACGCAGAGTACATGGG57490.2956636850011751No Hit
CATGTACTCTGCGTTGATACCACTG55290.28434936760680074No Hit
CTGCTTATGTACTCTGCGTTGATAC53090.2730350502124263No Hit
ACTCTGCGTTGATACCACTGCTTAT52040.26763503509238395No Hit
ACGCAGAGTACATAAGCAGTGGTAT50610.2602807287860406No Hit
GTACATGATAAGCAGTGGTATCAAC49480.25446928394256646No Hit
ATCATGTACTCTGCGTTGATACCAC47180.2426406793939023No Hit
GTATCAACGCAGAGTACATAAGCAG47080.24212639223961255No Hit
GAGTAAGCAGTGGTATCAACGCAGA43670.2245892002783322No Hit
TATCAACGCAGAGTACATAAGCAGT42370.2179034672725655No Hit
GTACATGGGAAGCAGTGGTATCAAC41870.21533203150111677No Hit
GGTATCAACGCAGAGTACATAAGCA38260.19676626523125693No Hit
GTACTGGTTCACTATCGGTCAGTCA36300.1866862370071779No Hit
AAGCAGTGGTATCAACGCAGAGTAC35350.18180050904142533No Hit
ATACAGGGTGACAGCCCCGTACACA32750.1684290430298919No Hit
GGATACCACGTGTCCCGCCCTACTC30160.1551090057337875No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28120.14461754778627667No Hit
GTGGTATCAACGCAGAGTACATAAG27760.14276611403083356No Hit
GTTAATGATAGTGTGTCGAAACACA27500.14142896742968025No Hit
GTACTCTGCGTTGATACCACTGCTT27220.13998896339766895No Hit
GCTTACTCTGCGTTGATACCACTGC27100.13937181881252125No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26560.13659466817935662No Hit
ACATAAGCAGTGGTATCAACGCAGA26200.13474323442391353No Hit
GCAGTGGTATCAACGCAGAGTACAT26140.13443466213133967No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24920.12816035884900479No Hit
ATCATTAACTGAATCCATAGGTTAA24670.1268746409632804No Hit
CCACTGCTTATGTACTCTGCGTTGA22470.11556032356890598No Hit
ACCCTGTATCGCGCGCCTTTCCAGA21450.11031459459515057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN20770.10681744194598031No Hit
GGTATCAACGCAGAGTACATGGGAA20560.10573743892197184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT453.5444424E-516.8862066
GTAGGCC402.7643636E-416.6227878
CCGAACT350.002171266816.2848111
TCCGTTA901.3460522E-1015.83245212
TAGGATC551.1247792E-515.551014
CTGATCG508.723691E-515.1983689
AGGCGCA456.771549E-414.7754297
GTAGTCG653.3751112E-614.61457112
AGTCGTG653.3760152E-614.61419410
GTCCTCG400.00526000914.25803851
TATACCG400.00526722314.2550944
CATAATC759.637333E-713.93831354
AGGGCCG551.9546105E-413.823125
CTTAACC551.9550386E-413.8227643
CGCCCCC551.9614708E-413.81741213
GGCCGTG551.9631891E-413.8159867
GAGGTAG551.9631891E-413.8159866
GTACGCT551.9631891E-413.8159866
CAGGGCC707.23799E-613.576284
TAGTCGA707.2689345E-613.57067413
Produced by FastQC (version 0.11.5)