##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295224_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1944439
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.521968032939064	31.0	30.0	33.0	25.0	34.0
2	29.64880050235569	31.0	30.0	34.0	24.0	34.0
3	29.772842449673146	31.0	30.0	34.0	25.0	34.0
4	32.84620808366835	35.0	33.0	37.0	22.0	37.0
5	33.34929509231197	35.0	33.0	37.0	27.0	37.0
6	33.468105196408835	36.0	35.0	37.0	27.0	37.0
7	33.47962162865485	36.0	35.0	37.0	27.0	37.0
8	33.56180317304889	37.0	35.0	37.0	27.0	37.0
9	34.29549757025034	38.0	34.0	39.0	22.0	39.0
10	34.41362932959069	38.0	34.0	39.0	23.0	39.0
11	34.769119524963244	39.0	35.0	39.0	26.0	39.0
12	34.83922200696448	39.0	35.0	39.0	26.0	39.0
13	34.63156776838975	39.0	35.0	39.0	25.0	39.0
14	35.20968515854702	39.0	34.0	41.0	23.0	41.0
15	35.52777999206969	39.0	34.0	41.0	25.0	41.0
16	35.46048654650519	39.0	34.0	41.0	25.0	41.0
17	35.28224233313568	39.0	34.0	41.0	24.0	41.0
18	35.16918761658247	39.0	34.0	41.0	23.0	41.0
19	35.594601322026556	39.0	34.0	41.0	25.0	41.0
20	35.61310948813514	39.0	34.0	41.0	25.0	41.0
21	35.65215828318605	39.0	34.0	41.0	25.0	41.0
22	35.56943879442863	39.0	34.0	41.0	25.0	41.0
23	35.301045185783664	39.0	34.0	41.0	24.0	41.0
24	35.62463929184716	39.0	34.0	41.0	25.0	41.0
25	34.454071842829734	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08850356571754858
1101	2	0.2833343275043134
1101	3	0.06390483596167229
1101	4	0.020119384449643007
1101	5	-0.058208714177801824
1101	6	-0.18755031724332127
1101	7	-0.18526005417312064
1101	8	-0.1443978863848301
1101	9	0.14344265385359023
1101	10	0.12402389382495471
1101	11	0.20302096923639823
1101	12	-0.13803963509067785
1101	13	0.05757364849657165
1101	14	0.36983608272970514
1101	15	0.0648524703090203
1101	16	0.1836237257685056
1101	17	0.2478227863242637
1101	18	0.31317686733837746
1101	19	0.039999122048726576
1101	20	0.012959299272210956
1101	21	-0.13243785997723734
1101	22	-0.2767092484083875
1101	23	-0.1734633430642134
1101	24	-0.14601350666471546
1101	25	-0.15748987641436685
1102	1	-0.17337048407428313
1102	2	0.1485815102601542
1102	3	0.08328918288716736
1102	4	0.0784123923708222
1102	5	-0.3318946834249843
1102	6	-0.1809782176894501
1102	7	-0.2053433287868458
1102	8	-0.15723049968946157
1102	9	-0.007705647497878942
1102	10	0.2099411495038055
1102	11	0.05638067364238708
1102	12	0.026831154090523057
1102	13	0.005934463299936965
1102	14	0.3073601456632886
1102	15	0.22392039549522025
1102	16	-0.03085497380050839
1102	17	-0.11800333375016692
1102	18	0.3216197789040436
1102	19	0.0016686200557245456
1102	20	-0.004472512470812262
1102	21	-0.07390902233497343
1102	22	-0.0122080555095323
1102	23	-0.016740743156987037
1102	24	-0.19233069738012887
1102	25	-0.06915348516429987
1103	1	-0.4038416063438781
1103	2	-0.02638704206414033
1103	3	-0.4740547515779383
1103	4	-0.25422230421065706
1103	5	-0.5835967244306914
1103	6	-0.5229330258328488
1103	7	-0.4949126245135673
1103	8	-0.3818862507476055
1103	9	-0.05706599661863265
1103	10	0.20913536725576165
1103	11	-0.04480647611556776
1103	12	-0.26085308546858954
1103	13	-0.3627101418770735
1103	14	-0.3983937479198332
1103	15	-0.4433071310161978
1103	16	-0.32189625274139644
1103	17	-0.16376949708217126
1103	18	0.11894595316540801
1103	19	-0.12554702335857115
1103	20	-0.2360450766936708
1103	21	-0.1702085073148467
1103	22	-0.26762002006130103
1103	23	-2.3624132078814597
1103	24	-0.9816566602718737
1103	25	-0.3911187844585342
1104	1	-0.3085870085455724
1104	2	0.002782641861671209
1104	3	-0.1990873632081147
1104	4	-0.5897050194158808
1104	5	-0.4678057567799172
1104	6	-0.3978481851315152
1104	7	-0.3103238590624713
1104	8	-0.3140816883994617
1104	9	-2.341862921411291
1104	10	-0.846664330471647
1104	11	-0.5221785464305313
1104	12	-0.28761556430572455
1104	13	-0.2055884430949959
1104	14	0.03611213226616883
1104	15	-0.19176990581807019
1104	16	-0.17526931717490868
1104	17	0.013149038182540096
1104	18	0.1182495655500091
1104	19	-0.07815216905748912
1104	20	-0.3480943486406005
1104	21	-0.40644602090827675
1104	22	-0.3701730628462414
1104	23	-0.18885625674717943
1104	24	-0.25214370266825625
1104	25	-0.28516530113272864
1105	1	-0.24179785654003183
1105	2	-0.21898535358533877
1105	3	-0.1859895976376329
1105	4	0.014518868936882257
1105	5	-0.1519229962820532
1105	6	-0.07450569632840853
1105	7	-0.23794937560417395
1105	8	-0.2747475632417178
1105	9	-2.006767531314047
1105	10	-0.4728041864585819
1105	11	-0.4873781109271036
1105	12	-0.2260170687721086
1105	13	-0.23169736844079836
1105	14	-0.2913485858195983
1105	15	-0.2259534606147966
1105	16	-0.157541358349782
1105	17	-1.5981661326783225
1105	18	-0.29697721791075793
1105	19	-0.2636501523796895
1105	20	-0.5352786705172292
1105	21	-0.3460350145489386
1105	22	-0.23552950755463087
1105	23	-0.14311998376571466
1105	24	-0.23966751926192842
1105	25	-0.8126453199385537
1106	1	-0.5087841944020397
1106	2	-0.42339453271537764
1106	3	-0.45420058062655855
1106	4	-0.38803850062585354
1106	5	-0.3663340782168305
1106	6	-0.3398693223131133
1106	7	-0.3310528501256158
1106	8	-0.4050112919778712
1106	9	-2.9832618038717946
1106	10	-0.7532106255497979
1106	11	-0.7220723302063234
1106	12	-0.34212697477355647
1106	13	-0.5031111523582936
1106	14	-0.5664492441900322
1106	15	-0.6031525320460815
1106	16	-0.5854730096037315
1106	17	-0.34938126378156653
1106	18	-0.1568835999178404
1106	19	-0.3621918428314146
1106	20	-0.3620579832241546
1106	21	-0.5061773443430582
1106	22	-0.41922247098272436
1106	23	-0.19201673758083615
1106	24	-0.30231926793947395
1106	25	-0.7784443076464385
1107	1	-0.19962304388897678
1107	2	0.009616179980984185
1107	3	-0.11935805346501027
1107	4	-1.2257833236108908
1107	5	-0.3224760886263951
1107	6	-0.22984866226654788
1107	7	-0.1205779986980815
1107	8	-0.09787207422184707
1107	9	0.2983554843519798
1107	10	0.44263128030545573
1107	11	0.0528277555726433
1107	12	-0.5286335207047159
1107	13	-0.08164190272000837
1107	14	0.25331818517130955
1107	15	-0.47004679603460886
1107	16	-0.709598110736195
1107	17	0.11677796691655118
1107	18	-0.03481805389566972
1107	19	-0.04511208272587197
1107	20	-0.3039626202329444
1107	21	-0.1829873557587547
1107	22	-0.11114197777875034
1107	23	0.0513518747706172
1107	24	-0.23516766705459702
1107	25	-0.09619338733201488
1108	1	0.02486881012663389
1108	2	0.03626185534607984
1108	3	0.0870885522826228
1108	4	0.42775650849031166
1108	5	0.3782524423144338
1108	6	0.19222476619152928
1108	7	0.260966094890243
1108	8	0.10541192639145436
1108	9	0.4140618147784565
1108	10	0.5188385567631073
1108	11	0.30359477583525774
1108	12	0.25560972548626637
1108	13	0.221348883552821
1108	14	0.30925747478646315
1108	15	0.042212408603710116
1108	16	0.060385276418955414
1108	17	0.2793777269708002
1108	18	0.2418425588037536
1108	19	0.16859865144806463
1108	20	0.20271176010478342
1108	21	-0.08408924102421622
1108	22	0.06829824364154291
1108	23	0.3020793782757565
1108	24	0.23433567053074
1108	25	0.23504208038042407
1109	1	0.1892670739408402
1109	2	0.10408992341571377
1109	3	0.26176003422258987
1109	4	0.5586790966044433
1109	5	0.5283707434114078
1109	6	0.37465152555370906
1109	7	0.3848351399128447
1109	8	0.2745983888752903
1109	9	0.67296939351926
1109	10	0.3750311516509157
1109	11	0.403171258409472
1109	12	0.3076536846597264
1109	13	0.22404732179784048
1109	14	0.5644331665808977
1109	15	0.33102211255264535
1109	16	0.4013915602403131
1109	17	0.5617042600592583
1109	18	0.3748684446581123
1109	19	0.21598507211663076
1109	20	0.27980827665264485
1109	21	0.2670196907462099
1109	22	0.2963351273113801
1109	23	0.5511444260800289
1109	24	0.3607185449240049
1109	25	0.20981093071637247
1110	1	0.3379703216309835
1110	2	0.27031939778629166
1110	3	0.32557828994287874
1110	4	0.5825365645975396
1110	5	0.434771211425101
1110	6	0.48702814445401543
1110	7	0.35422918575596185
1110	8	0.42454851378645486
1110	9	0.8695697271597069
1110	10	0.8760657634470874
1110	11	0.4544790660078206
1110	12	0.4692775336176709
1110	13	0.4159863649966269
1110	14	0.7056949810704367
1110	15	0.47869512004575654
1110	16	0.4535023820271178
1110	17	0.7953471932818204
1110	18	1.0604504055359882
1110	19	0.4776110971594889
1110	20	0.7491569550113653
1110	21	0.6931157672262671
1110	22	0.6487580035593723
1110	23	0.8032272502030153
1110	24	0.5939813433147876
1110	25	0.681565640094405
1111	1	0.20430036970635612
1111	2	0.09281890406891691
1111	3	0.16617457544456826
1111	4	0.5259296405761873
1111	5	0.4707975124414574
1111	6	0.4312268041961289
1111	7	0.3129203931138491
1111	8	0.21122735565626272
1111	9	0.5403881684228793
1111	10	0.4800343035105925
1111	11	0.2602288549696965
1111	12	0.04909486008406816
1111	13	0.14944209475260095
1111	14	0.14250380043583277
1111	15	0.1678517977011893
1111	16	0.07940694617418131
1111	17	0.3883078259071979
1111	18	0.5591870197302597
1111	19	0.32342420324257404
1111	20	-0.1262301061440141
1111	21	-0.08595205646375348
1111	22	-0.011321563591685901
1111	23	0.36533528504107693
1111	24	0.4053622584943355
1111	25	0.3627916625821399
1112	1	0.01681589094343039
1112	2	-0.03598956306582224
1112	3	0.20288325513513428
1112	4	0.5394149392169041
1112	5	0.4517170277721192
1112	6	0.4177797116774471
1112	7	0.3217970097332383
1112	8	0.2828114609954753
1112	9	-1.191972645440643
1112	10	0.11071765839199799
1112	11	0.03445471256232224
1112	12	-0.015177525801419733
1112	13	0.04908439222016625
1112	14	0.3258391674682457
1112	15	0.10332046936664341
1112	16	-0.021121769885326103
1112	17	0.06280126345732384
1112	18	0.22021471871172338
1112	19	0.1474100614932965
1112	20	0.12140342219592526
1112	21	0.2684089941314767
1112	22	0.30315065036625555
1112	23	0.023615707922523654
1112	24	0.22842148118255778
1112	25	0.24006208194916923
1113	1	0.2616911004310225
1113	2	0.08232151915689911
1113	3	0.14348774471913117
1113	4	0.3261548224174575
1113	5	0.5121710790452738
1113	6	0.25263378573905015
1113	7	0.21780717476392653
1113	8	0.25420453028488765
1113	9	-0.005623446079233929
1113	10	0.4309280372370594
1113	11	0.17048675105408506
1113	12	-0.07304284346078305
1113	13	0.11255644593876468
1113	14	-0.24967041123456823
1113	15	-0.013644713072608283
1113	16	0.10965330075671886
1113	17	0.5604544413457901
1113	18	0.3265666112538099
1113	19	0.17237679596424016
1113	20	0.36254503731882437
1113	21	0.4224963921607099
1113	22	0.4705856935672017
1113	23	0.13239543379643948
1113	24	0.24313462075362224
1113	25	0.22769641188884293
1114	1	0.15910858440121345
1114	2	0.14897343382870076
1114	3	0.25239738972430104
1114	4	0.4203601041347085
1114	5	0.4236580549731457
1114	6	0.32425536996860416
1114	7	0.09603075544634265
1114	8	0.07742361842497303
1114	9	-0.047242085589900285
1114	10	0.2481508208411185
1114	11	0.12826074805840904
1114	12	0.09883396326301153
1114	13	0.20089095302130033
1114	14	0.45812283720420055
1114	15	0.08545550449903061
1114	16	0.07299134775001903
1114	17	0.5334436848045883
1114	18	0.46657618787886435
1114	19	0.3213482500427318
1114	20	0.22826942639920134
1114	21	0.3335094054812444
1114	22	0.05974166280612536
1114	23	0.6214219538746875
1114	24	0.36326219224014267
1114	25	0.41698949840458255
1115	1	0.3294278764018159
1115	2	0.21421855543784218
1115	3	0.3228994860207237
1115	4	0.5945106886193088
1115	5	0.6154962596012012
1115	6	0.3623618263500603
1115	7	0.41520252997158735
1115	8	0.3743016209176844
1115	9	0.6281574580953659
1115	10	0.1114773333275636
1115	11	0.3941612495473521
1115	12	0.3066557039173148
1115	13	0.3352345742385481
1115	14	0.24433629666138046
1115	15	0.45954166771393545
1115	16	0.30893891393792927
1115	17	0.658456564781396
1115	18	0.8245858070538148
1115	19	0.4771129543880619
1115	20	0.5599273712316091
1115	21	0.4531241656663809
1115	22	-0.09877066623818109
1115	23	0.25252604220679586
1115	24	0.40081044500761465
1115	25	0.18882567645334092
1116	1	0.6041871030989228
1116	2	0.3527387676479137
1116	3	0.5841749940790315
1116	4	0.9232670712118178
1116	5	0.9090570967880467
1116	6	0.6958199521842587
1116	7	0.5866113499746106
1116	8	0.4195935614441382
1116	9	0.7424437939906596
1116	10	0.5939848949947049
1116	11	0.3797109061228383
1116	12	0.5113147924846757
1116	13	0.4637066536209744
1116	14	0.8494077903540713
1116	15	0.6297473010879742
1116	16	0.6761349107109496
1116	17	0.982622590440549
1116	18	0.9352734612298192
1116	19	0.7653732894708654
1116	20	0.6697465339512974
1116	21	0.7166537526353736
1116	22	0.7406814448885797
1116	23	1.0235007773559701
1116	24	0.6706404062238818
1116	25	0.6805831279407357
1201	1	-0.2345183709437073
1201	2	0.19896089846497844
1201	3	-0.1075114791277052
1201	4	0.07252960854808066
1201	5	-0.3056025916444369
1201	6	-0.33251631120545255
1201	7	-0.3377512977575847
1201	8	-0.06318919700974845
1201	9	0.19077882301828453
1201	10	0.39589489861635485
1201	11	0.027636888355672795
1201	12	0.11559449657923437
1201	13	-0.022904982213944436
1201	14	0.45358839224590497
1201	15	0.14390964364425685
1201	16	0.02299212965390751
1201	17	0.27100058076365485
1201	18	0.2912249262694573
1201	19	0.06225189388420915
1201	20	-0.009768913187535588
1201	21	-0.16910260175370695
1201	22	-0.025444127021891916
1201	23	0.015221426104503166
1201	24	-0.13188098930389458
1201	25	-0.10878230299341851
1202	1	-0.21263875188183334
1202	2	-0.18658365969544022
1202	3	-0.25666110398971753
1202	4	-0.05426516010123095
1202	5	-0.5132176121320242
1202	6	-0.3315171905812804
1202	7	-0.3589536103289177
1202	8	-0.307633225673662
1202	9	0.28648110752443046
1202	10	0.2816059768808046
1202	11	-0.018618280316090363
1202	12	-0.020439331325043497
1202	13	-0.0025745614232590697
1202	14	-0.08153612362963969
1202	15	-0.16542109521854087
1202	16	-0.048188666274768366
1202	17	0.16951686792663878
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2211	8	0.08532289229534484
2211	9	-1.557481943996038
2211	10	-0.1295913493304539
2211	11	-0.05112054961516321
2211	12	-0.04532522556261398
2211	13	0.0453481314002957
2211	14	0.23963547550042108
2211	15	0.08117763085351726
2211	16	0.24520726943706705
2211	17	0.37554414855131313
2211	18	0.4682242105914156
2211	19	0.1197127528877786
2211	20	0.32199844920870646
2211	21	0.1633848045720896
2211	22	0.19441463434755946
2211	23	-1.8506908298246714
2211	24	-0.6382670269350044
2211	25	-0.3934354130712947
2212	1	-0.0048368276931682885
2212	2	-1.3776980321022663
2212	3	-0.22004762813679335
2212	4	0.342700601011515
2212	5	0.3986010041810104
2212	6	0.3868376139568994
2212	7	0.26836978161701097
2212	8	0.38145614338537825
2212	9	0.7359262457200231
2212	10	0.6951066524262757
2212	11	0.4570454254182579
2212	12	0.45629446892035475
2212	13	0.33326669137881026
2212	14	0.6416696526379795
2212	15	0.39961068971303604
2212	16	0.33354231965146397
2212	17	0.7451393114960041
2212	18	0.803068878133189
2212	19	0.5588499791729475
2212	20	0.4435607194995086
2212	21	0.4652940880515928
2212	22	0.5012726057069301
2212	23	0.6230276288170344
2212	24	0.5195901028978511
2212	25	0.5424036702634041
2213	1	0.3257985728412969
2213	2	0.22554512655154824
2213	3	0.24825741825698344
2213	4	0.531866880564067
2213	5	0.49809597005270234
2213	6	0.3908630133423614
2213	7	0.29916526764318974
2213	8	0.2495006895877694
2213	9	0.4402928929546164
2213	10	0.6532685453120948
2213	11	0.38752736663283827
2213	12	0.44768837580289755
2213	13	0.3515475240489394
2213	14	-1.2316277207898025
2213	15	-0.1601755316560869
2213	16	0.2780213439406083
2213	17	0.5878301152858896
2213	18	0.43881534811063716
2213	19	0.4287276487624112
2213	20	0.28880383303896195
2213	21	0.4696522058522419
2213	22	0.6037019435761337
2213	23	0.7202397375116263
2213	24	0.42433870664361706
2213	25	0.2857878958859601
2214	1	0.210714274852279
2214	2	0.1327578341712865
2214	3	0.28444713248554976
2214	4	0.43163719667111167
2214	5	0.45092134862275657
2214	6	0.3595069949743177
2214	7	0.27115850046898515
2214	8	0.08546146997963433
2214	9	0.227581106237281
2214	10	0.0616937605365635
2214	11	0.06740352401130423
2214	12	0.038521938320087656
2214	13	0.07149785365699302
2214	14	0.09211539327708351
2214	15	0.0993843520018558
2214	16	0.34840862298653974
2214	17	0.2642526803115999
2214	18	0.5306898220295153
2214	19	0.2647634347796952
2214	20	0.25813678301020815
2214	21	0.3415797670859959
2214	22	0.36727004451704204
2214	23	0.4885709456855736
2214	24	0.5309334512494601
2214	25	0.4334539708415832
2215	1	0.29234383670452857
2215	2	0.06757108218434382
2215	3	0.1895676562902615
2215	4	0.41459088645624576
2215	5	0.38658589812654043
2215	6	0.24241469736736576
2215	7	0.2852624097934182
2215	8	0.119260579147209
2215	9	0.4144558846679658
2215	10	0.22816613938395136
2215	11	0.11116458762323589
2215	12	-0.058669028187544825
2215	13	0.017558009602183233
2215	14	0.2414624000365535
2215	15	0.007511365930767511
2215	16	0.143932992145281
2215	17	0.3189707055076241
2215	18	0.5458582147087654
2215	19	0.21034280264120753
2215	20	-0.7041224218951143
2215	21	-0.13655594127234139
2215	22	-0.7274968596125149
2215	23	0.13656245326794902
2215	24	0.16633590034545875
2215	25	0.5187611773154686
2216	1	0.04041681889127702
2216	2	-0.5173949888615645
2216	3	0.06325962622512904
2216	4	0.17159009865893182
2216	5	0.22159660998922703
2216	6	0.16259653029880639
2216	7	0.21448407578947126
2216	8	-0.02258647650382528
2216	9	0.4312102850074311
2216	10	0.04447372168758079
2216	11	0.04773603833417184
2216	12	-0.3253044314601894
2216	13	-0.2184675200729984
2216	14	-0.1102830307660767
2216	15	-0.07569455386300916
2216	16	0.033707797494692215
2216	17	0.17160566596811577
2216	18	0.49321182434317024
2216	19	0.2812522170859282
2216	20	-0.8945866330294336
2216	21	-1.129122558943017
2216	22	-1.0194203479205655
2216	23	-1.662983072670471
2216	24	-1.2961605935427514
2216	25	-1.0234548833097392
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3715.0
3	551.0
4	487.0
5	313.0
6	379.0
7	546.0
8	194.0
9	363.0
10	875.0
11	2010.0
12	4255.0
13	7554.0
14	11843.0
15	16017.0
16	19868.0
17	22027.0
18	22323.0
19	21422.0
20	20581.0
21	19433.0
22	18503.0
23	18025.0
24	17987.0
25	18067.0
26	19346.0
27	20566.0
28	22791.0
29	26709.0
30	32739.0
31	41404.0
32	54190.0
33	73046.0
34	98473.0
35	140720.0
36	212157.0
37	347301.0
38	607659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06472912751512	22.01331096813268	12.848951978851597	20.0730079255006
2	13.854079643553327	25.39868188990996	38.17021978815351	22.5770186783832
3	18.786326109391123	31.891434468524256	29.790712074303404	19.531527347781218
4	11.886940583362971	22.594230375156204	34.66136527519765	30.85746376628317
5	10.309369347791975	44.99498269753306	32.09287584344365	12.602772111231324
6	26.415770960706237	39.15098890994679	20.569977033533313	13.863263095813663
7	22.22096088661344	39.62980029215973	20.93008135786677	17.219157463360062
8	21.973765764203588	39.09150914719896	21.66396500588069	17.270760082716752
9	25.102637086819936	22.92037343593258	19.327352964245758	32.64963651300173
10	15.481803269041968	31.654249207412693	29.057628917384065	23.806318606161277
11	27.593635566558355	31.33034194646449	21.35865697044716	19.71736551652999
12	22.395814252285213	32.283972435571634	25.9781063516348	19.342106960508357
13	26.82606751124938	26.459195553792497	27.012082736022936	19.702654198935186
14	23.101357085554945	28.958879869166736	25.37123164348968	22.56853140178864
15	26.464634787069656	30.283982819823883	23.50274723856848	19.748635154537975
16	20.84410138006382	31.765093061715483	26.50832608585757	20.88247947236313
17	18.567603665421878	34.77789888505612	25.813397079800747	20.841100369721254
18	19.101502139833404	29.788441546045757	31.459549538626636	19.6505067754942
19	20.74565275834863	33.984002708012014	25.340913840531737	19.929430693107626
20	21.44185715435228	30.92420312909422	27.98343020196956	19.650509514583938
21	22.493845265511453	32.12902986110502	25.82279421591701	19.554330657466515
22	20.951970786354586	30.80461311519378	24.29415830247264	23.949257795978998
23	22.534272854414112	29.49296184295751	25.980587310689668	21.99217799193871
24	20.625480741204274	30.8966149112702	24.809934724952836	23.66796962257269
25	21.703756015552095	32.14219942357999	25.457708134620432	20.696336426247484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43768.0
1	43768.0
2	29398.5
3	15029.0
4	15029.0
5	15029.0
6	15375.0
7	15721.0
8	15721.0
9	15721.0
10	16974.5
11	18228.0
12	18228.0
13	18228.0
14	21790.0
15	25352.0
16	25352.0
17	25352.0
18	32777.5
19	40203.0
20	40203.0
21	40203.0
22	53055.5
23	65908.0
24	65908.0
25	65908.0
26	85545.0
27	105182.0
28	105182.0
29	105182.0
30	118825.0
31	132468.0
32	132468.0
33	132468.0
34	141560.0
35	150652.0
36	150652.0
37	150652.0
38	157248.0
39	163844.0
40	163844.0
41	163844.0
42	246432.0
43	329020.0
44	329020.0
45	329020.0
46	295036.0
47	261052.0
48	261052.0
49	261052.0
50	230912.5
51	200773.0
52	200773.0
53	200773.0
54	169090.5
55	137408.0
56	137408.0
57	137408.0
58	120090.0
59	102772.0
60	102772.0
61	102772.0
62	87755.0
63	72738.0
64	72738.0
65	72738.0
66	54077.0
67	35416.0
68	35416.0
69	35416.0
70	26632.5
71	17849.0
72	17849.0
73	17849.0
74	12811.0
75	7773.0
76	7773.0
77	7773.0
78	5053.5
79	2334.0
80	2334.0
81	2334.0
82	1509.0
83	684.0
84	684.0
85	684.0
86	452.5
87	221.0
88	221.0
89	221.0
90	128.5
91	36.0
92	36.0
93	36.0
94	19.0
95	2.0
96	2.0
97	2.0
98	4.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1405032505519587
2	0.27252076305813655
3	0.33114949864716764
4	0.3644238775297142
5	0.3673553143091658
6	0.3289380638837217
7	0.3321266442403182
8	0.34673239942214695
9	0.33649808505178097
10	0.36581245284629654
11	0.2848636547610905
12	0.3308923550700228
13	0.3028122764458026
14	0.3134065918241714
15	0.35264670169647905
16	0.299983697097209
17	0.3478124024461554
18	0.3190637505213586
19	0.33356664827232946
20	0.2947379681234536
21	0.3342866502883351
22	0.28897795199540843
23	0.27231504819642066
24	0.30990943917500113
25	0.30456085277038775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1944439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.02979811273186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14211022947521	15.651706919992279
2	7.35519451259398	2.946461223342613
3	2.908906825245809	1.7479444931526398
4	1.6646958498198021	1.3337408716397297
5	1.167702021053273	1.1694417868763014
6	0.8998544539099356	1.0814341825595155
7	0.6843591602798791	0.959530307190328
8	0.5661490924482773	0.907188161875629
9	0.4905583045777723	0.8843205422895123
>10	4.76299779241977	20.46115768236376
>50	0.8140234208550445	11.31990244447985
>100	0.48602542880450716	17.685044069299547
>500	0.02922877775851524	4.21744273194417
>1k	0.023796881006932766	8.818223409340657
>5k	0.0031039410009042743	4.165115211345868
>10k+	0.0012933087503767809	6.651345962307588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	40801	2.098343018217594	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	37943	1.9513597495215844	No Hit
GCTTATGTACTCTGCGTTGATACCA	23944	1.2314091622313685	No Hit
CTTATGTACTCTGCGTTGATACCAC	15506	0.7974536614416806	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10187	0.5239043240749646	No Hit
GAGTACATAAGCAGTGGTATCAACG	9658	0.49669853361303695	No Hit
TATGTACTCTGCGTTGATACCACTG	8279	0.42577833503648094	No Hit
CCCATGTACTCTGCGTTGATACCAC	7917	0.40716114005119214	No Hit
GCGTTGATACCACTGCTTATGTACT	7302	0.3755324800623727	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7127	0.3665324548623022	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6728	0.3460123974061413	No Hit
CATAAGCAGTGGTATCAACGCAGAG	6530	0.33582951175120435	No Hit
GTGGTATCAACGCAGAGTACATGGG	5749	0.2956636850011751	No Hit
CATGTACTCTGCGTTGATACCACTG	5529	0.28434936760680074	No Hit
CTGCTTATGTACTCTGCGTTGATAC	5309	0.2730350502124263	No Hit
ACTCTGCGTTGATACCACTGCTTAT	5204	0.26763503509238395	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	5061	0.2602807287860406	No Hit
GTACATGATAAGCAGTGGTATCAAC	4948	0.25446928394256646	No Hit
ATCATGTACTCTGCGTTGATACCAC	4718	0.2426406793939023	No Hit
GTATCAACGCAGAGTACATAAGCAG	4708	0.24212639223961255	No Hit
GAGTAAGCAGTGGTATCAACGCAGA	4367	0.2245892002783322	No Hit
TATCAACGCAGAGTACATAAGCAGT	4237	0.2179034672725655	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4187	0.21533203150111677	No Hit
GGTATCAACGCAGAGTACATAAGCA	3826	0.19676626523125693	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3630	0.1866862370071779	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3535	0.18180050904142533	No Hit
ATACAGGGTGACAGCCCCGTACACA	3275	0.1684290430298919	No Hit
GGATACCACGTGTCCCGCCCTACTC	3016	0.1551090057337875	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2812	0.14461754778627667	No Hit
GTGGTATCAACGCAGAGTACATAAG	2776	0.14276611403083356	No Hit
GTTAATGATAGTGTGTCGAAACACA	2750	0.14142896742968025	No Hit
GTACTCTGCGTTGATACCACTGCTT	2722	0.13998896339766895	No Hit
GCTTACTCTGCGTTGATACCACTGC	2710	0.13937181881252125	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2656	0.13659466817935662	No Hit
ACATAAGCAGTGGTATCAACGCAGA	2620	0.13474323442391353	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2614	0.13443466213133967	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2492	0.12816035884900479	No Hit
ATCATTAACTGAATCCATAGGTTAA	2467	0.1268746409632804	No Hit
CCACTGCTTATGTACTCTGCGTTGA	2247	0.11556032356890598	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2145	0.11031459459515057	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	2077	0.10681744194598031	No Hit
GGTATCAACGCAGAGTACATGGGAA	2056	0.10573743892197184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.142871542897463E-5	0.0	0.0	5.142871542897463E-5	0.0
2	5.142871542897463E-5	0.0	0.0	5.142871542897463E-5	0.0
3	5.142871542897463E-5	0.0	0.0	5.142871542897463E-5	0.0
4	5.142871542897463E-5	0.0	0.0	5.142871542897463E-5	0.0
5	1.5428614628692388E-4	0.0	0.0	5.142871542897463E-5	0.0
6	1.5428614628692388E-4	0.0	0.0	5.142871542897463E-5	0.0
7	1.5428614628692388E-4	0.0	0.0	5.142871542897463E-5	0.0
8	1.5428614628692388E-4	0.0	0.0	5.142871542897463E-5	0.0
9	1.5428614628692388E-4	0.0	0.0	5.142871542897463E-5	0.0
10	1.5428614628692388E-4	0.0	0.0	1.0285743085794926E-4	0.0
11	1.5428614628692388E-4	0.0	0.0	1.5428614628692388E-4	0.0
12	1.5428614628692388E-4	0.0	0.0	1.5428614628692388E-4	0.0
13	1.5428614628692388E-4	0.0	0.0	2.5714357714487317E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCCGT	45	3.5444424E-5	16.886206	6
GTAGGCC	40	2.7643636E-4	16.622787	8
CCGAACT	35	0.0021712668	16.28481	11
TCCGTTA	90	1.3460522E-10	15.832452	12
TAGGATC	55	1.1247792E-5	15.55101	4
CTGATCG	50	8.723691E-5	15.198368	9
AGGCGCA	45	6.771549E-4	14.775429	7
GTAGTCG	65	3.3751112E-6	14.614571	12
AGTCGTG	65	3.3760152E-6	14.614194	10
GTCCTCG	40	0.005260009	14.2580385	1
TATACCG	40	0.005267223	14.255094	4
CATAATC	75	9.637333E-7	13.9383135	4
AGGGCCG	55	1.9546105E-4	13.82312	5
CTTAACC	55	1.9550386E-4	13.822764	3
CGCCCCC	55	1.9614708E-4	13.817412	13
GGCCGTG	55	1.9631891E-4	13.815986	7
GAGGTAG	55	1.9631891E-4	13.815986	6
GTACGCT	55	1.9631891E-4	13.815986	6
CAGGGCC	70	7.23799E-6	13.57628	4
TAGTCGA	70	7.2689345E-6	13.570674	13
>>END_MODULE