FastQCFastQC Report
Wed 25 May 2016
SRR1295224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944439
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA419852.1592346172855No Hit
GTATCAACGCAGAGTACTTTTTTTT338371.7401934439702147No Hit
GCTTATGTACTCTGCGTTGATACCA264971.362706672721541No Hit
GGTATCAACGCAGAGTACTTTTTTT244941.2596949557173045No Hit
TATCAACGCAGAGTACTTTTTTTTT222931.1465003530581315No Hit
CTTATGTACTCTGCGTTGATACCAC177600.9133739860185894No Hit
GTACTTTTTTTTTTTTTTTTTTTTT125770.6468189539502139No Hit
ACGCAGAGTACTTTTTTTTTTTTTT115430.5936416621966542No Hit
GAGTACATAAGCAGTGGTATCAACG99700.5127442928268771No Hit
CCCATGTACTCTGCGTTGATACCAC95590.49160709078556847No Hit
GCGTTGATACCACTGCTTATGTACT81070.4169325959826973No Hit
TATGTACTCTGCGTTGATACCACTG80060.4117382957243709No Hit
CATAAGCAGTGGTATCAACGCAGAG77460.3983668297128375No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69580.3578410019548055No Hit
CATGTACTCTGCGTTGATACCACTG63090.32446376564140095No Hit
CTGCTTATGTACTCTGCGTTGATAC62410.32096661299223067No Hit
GTGGTATCAACGCAGAGTACATGGG59510.30605228551782804No Hit
ACTCTGCGTTGATACCACTGCTTAT58600.30137227241379133No Hit
ACGCAGAGTACATAAGCAGTGGTAT57310.2947379681234536No Hit
ATCATGTACTCTGCGTTGATACCAC52610.2705664718718355No Hit
GTATCAACGCAGAGTACATAAGCAG49980.2570407197140152No Hit
GTACATGATAAGCAGTGGTATCAAC49170.25287499376426825No Hit
GCAGAGTACTTTTTTTTTTTTTTTT48360.2487092678145213No Hit
GAGTAAGCAGTGGTATCAACGCAGA46810.24073781692303026No Hit
TATCAACGCAGAGTACATAAGCAGT46530.23929781289101895No Hit
GTACATGGGAAGCAGTGGTATCAAC42800.22011490203601142No Hit
GGTATCAACGCAGAGTACATAAGCA42140.2167206068176991No Hit
AAGCAGTGGTATCAACGCAGAGTAC40060.20602343400847237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39150.20134342090443566No Hit
GTACTGGTTCACTATCGGTCAGTCA38020.19553197606096154No Hit
ATACAGGGTGACAGCCCCGTACACA34450.17717192465281761No Hit
ATCAACGCAGAGTACTTTTTTTTTT33370.17161762338648834No Hit
GTGGTATCAACGCAGAGTACTTTTT33280.17115476494762755No Hit
ACATAAGCAGTGGTATCAACGCAGA32760.16848047174532088No Hit
GGATACCACGTGTCCCGCCCTACTC31280.16086902186183263No Hit
GCTTACTCTGCGTTGATACCACTGC30910.15896615939096057No Hit
GTTAATGATAGTGTGTCGAAACACA30470.1567032959120857No Hit
GTGGTATCAACGCAGAGTACATAAG28190.1449775487942795No Hit
GCAGTGGTATCAACGCAGAGTACAT27810.14302325760797843No Hit
GTACTCTGCGTTGATACCACTGCTT26250.1350003780010584No Hit
ATCATTAACTGAATCCATAGGTTAA25490.13109179562845633No Hit
CCACTGCTTATGTACTCTGCGTTGA24620.12661749738613554No Hit
ACCCTGTATCGCGCGCCTTTCCAGA23310.11988033566493986No Hit
CCCATATTCAGACAGGATACCACGT22810.11730889989349112No Hit
GGTATCAACGCAGAGTACATGGGAA22590.11617746815405369No Hit
GATACCACTGCTTATGTACTCTGCG22180.11406889082146573No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21700.11160031248087494No Hit
GCGTTGATACCACTGCTTACTCTGC20660.1062517260762616No Hit
ATACCACTGCTTATGTACTCTGCGT20410.10496600819053722No Hit
GAGTACATGATAAGCAGTGGTATCA19490.10023456637107155No Hit
GTTGATACCACTGCTTATGTACTCT19480.10018313765564257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGGG250.006034215418.9991263
CGATGCG402.759825E-416.6263739
GGCTGCG350.002170011916.2862227
CCGCGCG508.709599E-515.2012559
CTAGGAC704.439953E-714.9278843
GCGCGAA400.005276841614.25117711
AGGTCCG602.5669156E-514.2511768
CTTATTC400.005281345514.2493442
CCTGATC602.5700301E-514.2493443
TTCGACC551.961744E-413.8171917
TAGGATC903.6427082E-813.7215914
GCCTAGT1400.013.5722011
GATGCTC1550.013.48498410
GGCTACG500.00149885113.3010988
GCGCAGA500.001499141613.300755513
GTATCTA655.4496377E-513.1549329
TATAGGA1102.750312E-912.9539492
AAATCGC2200.012.95361617
GGTACCG905.401889E-712.6677139
AGTATCT604.0899974E-412.6677128
Produced by FastQC (version 0.11.5)