FastQCFastQC Report
Wed 25 May 2016
SRR1295223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15609
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA790.5061182651034659No Hit
GCTTATGTACTCTGCGTTGATACCA430.27548209366391185No Hit
CTTATGTACTCTGCGTTGATACCAC250.1601640079441348No Hit
ATTCCATTCCATTCCATTCCATTCC240.1537574476263694No Hit
TATGTACTCTGCGTTGATACCACTG200.12813120635530784No Hit
GAGTACATAAGCAGTGGTATCAACG200.12813120635530784No Hit
GAATGGAATGGAATGGAATGGAATG200.12813120635530784No Hit
GTGGTATCAACGCAGAGTACATGGG170.10891152540201167No Hit
ACTCTGCGTTGATACCACTGCTTAT160.10250496508424627No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)