##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295223_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15609
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.455954897815364	31.0	30.0	33.0	27.0	34.0
2	30.737651354987506	31.0	31.0	34.0	27.0	34.0
3	30.84515343711961	31.0	31.0	34.0	27.0	34.0
4	34.319559228650135	37.0	35.0	37.0	30.0	37.0
5	34.461400474085465	37.0	35.0	37.0	31.0	37.0
6	34.6124031007752	37.0	35.0	37.0	32.0	37.0
7	34.6860785444295	37.0	35.0	37.0	32.0	37.0
8	34.59766801204433	37.0	35.0	37.0	31.0	37.0
9	35.82170542635659	39.0	35.0	39.0	30.0	39.0
10	35.86309180600935	39.0	35.0	39.0	30.0	39.0
11	36.195848548914086	39.0	37.0	39.0	31.0	39.0
12	36.195848548914086	39.0	37.0	39.0	31.0	39.0
13	36.17733358959575	39.0	37.0	39.0	31.0	39.0
14	36.91050035236082	40.0	36.0	41.0	30.0	41.0
15	37.247613556281635	40.0	37.0	41.0	31.0	41.0
16	37.234416042027036	40.0	37.0	41.0	31.0	41.0
17	36.88064578128003	40.0	36.0	41.0	30.0	41.0
18	36.76539176116343	40.0	36.0	41.0	29.0	41.0
19	37.177653917611636	40.0	37.0	41.0	30.0	41.0
20	37.23588955090012	40.0	37.0	41.0	31.0	41.0
21	37.30027548209367	40.0	37.0	41.0	31.0	41.0
22	37.219873150105705	40.0	37.0	41.0	31.0	41.0
23	36.93029662374271	40.0	37.0	41.0	30.0	41.0
24	37.264270613107826	40.0	37.0	41.0	31.0	41.0
25	36.1109616247037	39.0	35.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2153380580525308
1101	2	0.3778832743655052
1101	3	0.1114891084367109
1101	4	-0.7204974065251193
1101	5	0.003046125791776433
1101	6	0.47505827976461745
1101	7	0.1284520762618513
1101	8	0.745222461091295
1101	9	0.5302508233177576
1101	10	1.9569133965542846
1101	11	0.26450283461657875
1101	12	0.09240512309691695
1101	13	0.12069539725747802
1101	14	0.6221218107687889
1101	15	0.7582311902586412
1101	16	1.755663943144846
1101	17	1.4473555126036004
1101	18	2.2938278693023975
1101	19	1.1239584021856075
1101	20	-0.19802707563315636
1101	21	-0.25995249848502766
1101	22	0.7579227321432356
1101	23	1.4525805353449854
1101	24	-0.08020085033481195
1101	25	-0.9324376046669514
1102	1	0.05533805805253067
1102	2	0.9378832743655074
1102	3	0.15148910843671004
1102	4	0.7995025934748838
1102	5	-0.23695387420822556
1102	6	0.5150582797646166
1102	7	-0.15154792373814985
1102	8	0.5052224610913001
1102	9	1.0102508233177545
1102	10	1.1169133965542883
1102	11	0.4645028346165745
1102	12	-0.02759487690308049
1102	13	0.8806953972574831
1102	14	-0.33787818923121193
1102	15	-0.6017688097413583
1102	16	-2.1243360568551495
1102	17	-2.432644487396402
1102	18	0.9738278693023972
1102	19	0.0439584021856092
1102	20	-0.6780270756331603
1102	21	0.8600475015149698
1102	22	-2.2820772678567707
1102	23	0.012580535344980603
1102	24	-1.120200850334811
1102	25	-0.25243760466695164
1103	1	0.7337995965140713
1103	2	0.2609601974424294
1103	3	1.15148910843671
1103	4	1.5302718242441173
1103	5	1.4138153565610097
1103	6	1.131981356687696
1103	7	1.4069136147233934
1103	8	0.5467609226297583
1103	9	0.945635438702368
1103	10	1.6953749350158276
1103	11	1.9106566807704226
1103	12	1.241635892327686
1103	13	1.768387704949788
1103	14	1.4144295030764837
1103	15	0.7597696517971002
1103	16	1.495663943144848
1103	17	1.5935093587574443
1103	18	1.603058638533163
1103	19	0.3485737868009977
1103	20	1.0758190782129944
1103	21	1.9077398092072784
1103	22	1.2640765782970789
1103	23	-3.7474194646550174
1103	24	-1.4663546964886578
1103	25	0.49371624148689364
1104	1	-0.08243971972524733
1104	2	0.7452906817729144
1104	3	-0.1818442248966221
1104	4	-0.644941850969559
1104	5	-1.4280649853193381
1104	6	-0.5560528313464985
1104	7	0.3314150392248152
1104	8	0.02966690553574125
1104	9	-0.8549343618674285
1104	10	-0.2134569738160863
1104	11	-0.3058675357537979
1104	12	-1.28685413616234
1104	13	1.1458805824426648
1104	14	1.3702699589169356
1104	15	-0.2017688097413597
1104	16	-0.6339656864847782
1104	17	0.35846662371471183
1104	18	0.2967908322653585
1104	19	0.9269213651485728
1104	20	0.8108618132557268
1104	21	0.24819564966311702
1104	22	-0.30429949007898927
1104	23	0.9192472020116469
1104	24	1.1390584089244484
1104	25	0.7401549879256422
1105	1	-0.3916184636865978
1105	2	-0.662116725634494
1105	3	-0.6745978480850283
1105	4	-1.5483234934816394
1105	5	-2.4908669176864855
1105	6	-2.097115633278861
1105	7	-1.8054609672164048
1105	8	-1.5017340606478342
1105	9	-2.495836133203987
1105	10	-1.3213474730109311
1105	11	-1.5007145566877753
1105	12	-2.940638355163948
1105	13	-2.0393046027425186
1105	14	-2.4300521022746864
1105	15	-1.8104644619152737
1105	16	-1.0478143177247148
1105	17	-2.013514052613793
1105	18	-2.709650391567166
1105	19	-0.5143024673796077
1105	20	-1.7736792495462055
1105	21	-1.8999524984850282
1105	22	-0.2559903113350259
1105	23	-2.225680334220236
1105	24	0.1406687148825796
1105	25	-0.8959158655365158
1106	1	0.8620047247191991
1106	2	1.087883274365506
1106	3	0.6098224417700422
1106	4	1.2161692601415481
1106	5	1.1830461257917761
1106	6	1.59672494643128
1106	7	1.201785409595189
1106	8	1.543555794424634
1106	9	-0.8364158433489095
1106	10	1.7819133965542875
1106	11	0.8561695012832402
1106	12	2.264071789763584
1106	13	2.044028730590817
1106	14	2.2221218107687903
1106	15	1.631564523591976
1106	16	1.4539972764781837
1106	17	1.7890221792702619
1106	18	2.500494535969061
1106	19	1.963958402185611
1106	20	1.0886395910335054
1106	21	1.9333808348483075
1106	22	2.126256065476568
1106	23	2.2109138686783183
1106	24	1.5881324829985246
1106	25	0.9392290619997112
1107	1	-0.9133575941213827
1107	2	-1.2273341169388416
1107	3	-1.152858717650247
1107	4	-2.504845232612073
1107	5	-1.490866917686482
1107	6	-1.8362460680614703
1107	7	-1.8054609672164048
1107	8	-1.4582557997782644
1107	9	-2.1914883071170266
1107	10	-2.147434429532666
1107	11	-3.4572362958182055
1107	12	-3.158029659511776
1107	13	-4.169739385351214
1107	14	-3.4300521022746864
1107	15	-2.0278557662631016
1107	16	-4.3086838829421055
1107	17	-2.4917749221790118
1107	18	-2.2748677828715174
1107	19	-2.7751720325969984
1107	20	-1.251940119111417
1107	21	-3.508648150658942
1107	22	-3.1690337895959004
1107	23	-2.5735064211767593
1107	24	-1.8158530242478577
1107	25	-2.287220213362602
1108	1	-0.5738927111782388
1108	2	-0.5851936487114173
1108	3	0.07456603151363339
1108	4	-1.3928050988328096
1108	5	-1.432338489592837
1108	6	-0.32955710485077105
1108	7	-1.0930863852766066
1108	8	-1.7993929235240884
1108	9	-0.554364561297632
1108	10	-0.8815481419072526
1108	11	-0.9739587038449642
1108	12	-1.296825646133847
1108	13	-1.231612295050212
1108	14	-0.5855704969235163
1108	15	-1.5863841943567465
1108	16	-1.2351052876243855
1108	17	-0.25264448739640244
1108	18	-0.3584798230052968
1108	19	-0.1898877516605424
1108	20	-0.3472578448639254
1108	21	0.8308167322842053
1108	22	-0.12053880631830793
1108	23	-0.3243425415780976
1108	24	-1.3125085426425045
1108	25	-2.4678222200515663
1109	1	0.8692511015307929
1109	2	0.16397023088724438
1109	3	0.455836934523667
1109	4	0.7125460717357512
1109	5	-0.14304083072996576
1109	6	1.2072321928080925
1109	7	0.8901912066966347
1109	8	-0.06695145195217833
1109	9	0.37372908418731754
1109	10	-0.14743442953266594
1109	11	0.10798109548613866
1109	12	0.6680572970099661
1109	13	1.4389562668227
1109	14	0.2656000716383531
1109	15	0.1460572772151636
1109	16	-0.09129257859428463
1109	17	0.4647468169514255
1109	18	0.46426265191109195
1109	19	-2.079519858683952
1109	20	-1.6867227278070729
1109	21	-2.552126411528505
1109	22	-0.08207726785676783
1109	23	0.5134501005623733
1109	24	-0.25063563294350644
1109	25	-0.7220028220582577
1110	1	1.0799534426679145
1110	2	0.4532678897501228
1110	3	0.88225833920594
1110	4	1.6071949011671904
1110	5	1.144584587330236
1110	6	1.0935198182261558
1110	7	1.0992213070310868
1110	8	1.7006070764759116
1110	9	2.2533277463946746
1110	10	1.887682627323521
1110	11	1.7952720653858094
1110	12	1.9339435846353794
1110	13	2.306849243411328
1110	14	2.529814118461097
1110	15	0.4136158056432535
1110	16	1.9187408662217678
1110	17	1.7858170510651377
1110	18	1.9107509462254768
1110	19	1.4254968637240708
1110	20	1.383511385905301
1110	21	1.676970578438052
1110	22	1.0717688859893855
1110	23	2.2141189968834425
1110	24	1.6105683804344224
1110	25	1.8783316261022804
1111	1	0.4261072888217612
1111	2	0.10711404359627608
1111	3	0.8437968007444034
1111	4	1.2610410550133437
1111	5	1.2215076642533162
1111	6	0.7858275105338492
1111	7	0.9069136147233934
1111	8	1.5467609226297583
1111	9	1.868712361779295
1111	10	1.464605704246594
1111	11	0.9875797576934957
1111	12	0.8570205077123063
1111	13	1.191464628026715
1111	14	1.9528910415380238
1111	15	1.144385036412487
1111	16	1.1495100969910013
1111	17	2.0935093587574443
1111	18	0.9876740231485499
1111	19	1.002419940647151
1111	20	0.4219729243668411
1111	21	-0.16918326771579473
1111	22	0.6871535013739987
1111	23	0.5218113045757491
1111	24	0.4567222265882691
1111	25	1.6091008568715068
1112	1	1.41533805805253
1112	2	0.6578832743655063
1112	3	1.2714891084367075
1112	4	2.079502593474885
1112	5	1.5630461257917787
1112	6	-0.36494172023538596
1112	7	0.2084520762618567
1112	8	-0.014777538908703036
1112	9	-1.5097491766822486
1112	10	0.5969133965542852
1112	11	1.1445028346165742
1112	12	0.8124051230969229
1112	13	-0.07930460274251772
1112	14	1.8221218107687918
1112	15	1.1182311902586406
1112	16	-0.044336056855151185
1112	17	0.2873555126035967
1112	18	0.13382786930239376
1112	19	0.08395840218560835
1112	20	0.6819729243668391
1112	21	1.4600475015149712
1112	22	0.5579227321432327
1112	23	1.2525805353449826
1112	24	1.3597991496651858
1112	25	0.5075623953330464
1113	1	-0.06466194194747032
1113	2	-0.2621167256344954
1113	3	-0.48851089156329053
1113	4	-0.5604974065251156
1113	5	0.6830461257917761
1113	6	-0.5649417202353817
1113	7	0.008452076261853847
1113	8	-0.5347775389086991
1113	9	-1.1897491766822483
1113	10	-0.7630866034457142
1113	11	-1.2554971653834244
1113	12	-1.4675948769030782
1113	13	-0.7593046027425174
1113	14	-2.057878189231211
1113	15	-1.1217688097413614
1113	16	-2.284336056855153
1113	17	-0.632644487396405
1113	18	0.45382786930239405
1113	19	-1.7160415978143888
1113	20	-1.878027075633156
1113	21	-1.339952498485026
1113	22	-0.4820772678567664
1113	23	-0.34741946465501883
1113	24	-1.2002008503348094
1113	25	-2.052437604666956
1114	1	0.5753380580525302
1114	2	1.6978832743655055
1114	3	2.191489108436709
1114	4	1.7595025934748847
1114	5	1.9630461257917773
1114	6	1.7150582797646123
1114	7	1.4084520762618524
1114	8	1.8652224610912995
1114	9	1.2102508233177574
1114	10	1.5969133965542852
1114	11	1.1445028346165742
1114	12	1.1724051230969224
1114	13	1.36069539725748
1114	14	2.142121810768792
1114	15	1.8382311902586395
1114	16	2.3556639431448474
1114	17	2.7273555126035944
1114	18	2.413827869302395
1114	19	1.6039584021856115
1114	20	2.441972924366844
1114	21	2.700047501514973
1114	22	2.35792273214323
1114	23	3.092580535344986
1114	24	2.399799149665192
1114	25	2.5475623953330455
1115	1	0.7786713913858634
1115	2	0.3795499410321739
1115	3	-0.1401775582299578
1115	4	1.3828359268082195
1115	5	0.7247127924584404
1115	6	0.59672494643128
1115	7	0.2851187429285176
1115	8	-1.1231108722420373
1115	9	-0.044749176682245206
1115	10	-0.6764199367790482
1115	11	-0.9354971653834241
1115	12	-0.1942615435697448
1115	13	0.29402873059081713
1115	14	0.30545514410212604
1115	15	-0.243435476408024
1115	16	-1.004336056855152
1115	17	0.33068884593693326
1115	18	0.5004945359690609
1115	19	-1.036041597814389
1115	20	-0.6613604089664946
1115	21	1.1833808348483075
1115	22	0.16792273214323217
1115	23	0.8775805353449826
1115	24	0.1297991496651889
1115	25	-0.9357709380002888
1116	1	1.7298208166732216
1116	2	0.9585729295379188
1116	3	1.1170063498160197
1116	4	1.5236405245093678
1116	5	0.9933909533779826
1116	6	-0.16907965126986824
1116	7	0.3339693176411629
1116	8	0.7576362541947432
1116	9	0.46818185780051635
1116	10	-0.1879141896526093
1116	11	0.7886407656510599
1116	12	0.8689568472348483
1116	13	0.4779367765678231
1116	14	1.7048804314584487
1116	15	0.7637484316379499
1116	16	1.54738808107588
1116	17	1.4887348229484232
1116	18	1.2303795934403254
1116	19	0.5846480573580237
1116	20	1.094386717470286
1116	21	1.306944053239107
1116	22	-0.42690485406366463
1116	23	0.14913225948291142
1116	24	0.2935922531134665
1116	25	1.1130796367123565
1201	1	-0.08243971972524733
1201	2	-0.6991537626715321
1201	3	-1.9225849656373626
1201	4	-0.4967937028214138
1201	5	0.6830461257917761
1201	6	-0.22271949801316282
1201	7	0.257340965150739
1201	8	-0.4888516129827778
1201	9	0.2932137862807167
1201	10	1.1939504335913256
1201	11	0.24968801980175925
1201	12	1.231664382356179
1201	13	-0.15041571385363284
1201	14	-0.40750781886083587
1201	15	-0.31287992085247396
1201	16	0.2178861653670694
1201	17	0.09920736445545231
1201	18	0.7782723137468395
1201	19	-0.6656712274440153
1201	20	0.1812321836261006
1201	21	1.1370845385520099
1201	22	1.843848658069156
1201	23	0.7710990538635016
1201	24	1.4723917422577841
1201	25	1.2957105434811922
1202	1	-1.2213286086141366
1202	2	-1.3704500589678261
1202	3	-3.056844224896622
1202	4	-0.9504974065251162
1202	5	-4.191953874208224
1202	6	-1.52827505356872
1202	7	-3.7982145904048146
1202	8	-3.2064442055753695
1202	9	-1.6697491766822452
1202	10	-2.509753270112377
1202	11	-1.9771638320500884
1202	12	-1.9025948769030805
1202	13	-2.8726379360758543
1202	14	-3.7778781892312097
1202	15	-2.2017688097413597
1202	16	-2.9210027235218163
1202	17	-1.1693111540630667
1202	18	-3.9161721306976034
1202	19	-2.744374931147725
1202	20	-2.369693742299823
1202	21	-1.4416191651516925
1202	22	-2.498743934523432
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2211	21	0.10004750151497177
2211	22	-0.602077267856771
2211	23	-2.387419464655018
2211	24	-0.5202008503348097
2211	25	0.5075623953330464
2212	1	0.2722611349756079
2212	2	-0.431347494865264
2212	3	0.11302756997516994
2212	4	0.6841179780902706
2212	5	0.6830461257917761
2212	6	0.5165967413030756
2212	7	0.21460592241569998
2212	8	1.5082993841682182
2212	9	1.7148662079331416
2212	10	1.7722980119389007
2212	11	1.4875797576934957
2212	12	0.8954820461738393
2212	13	1.3068492434113281
2212	14	1.4144295030764837
2212	15	1.2982311902586403
2212	16	1.7648947123756145
2212	17	2.555047820295904
2212	18	2.641520176994703
2212	19	1.579343017570224
2212	20	1.4988960012899213
2212	21	1.176970578438052
2212	22	0.41792273214323217
2212	23	1.444888227652676
2212	24	1.3413376112036488
2212	25	0.686023933794587
2213	1	0.7753380580525295
2213	2	0.337883274365506
2213	3	-0.008510891563290102
2213	4	0.9995025934748867
2213	5	0.7630461257917744
2213	6	0.9950582797646135
2213	7	1.2084520762618567
2213	8	1.5852224610912984
2213	9	1.7302508233177534
2213	10	1.6769133965542906
2213	11	0.7845028346165748
2213	12	0.6124051230969201
2213	13	0.8006953972574848
2213	14	-1.377878189231211
2213	15	0.3582311902586426
2213	16	1.035663943144847
2213	17	1.0073555126035956
2213	18	-0.06617213069760197
2213	19	0.1239584021856075
2213	20	0.3619729243668388
2213	21	2.0200475015149735
2213	22	0.9579227321432313
2213	23	2.6925805353449803
2213	24	1.15979914966519
2213	25	-0.41243760466695534
2214	1	-0.5460412522922979
2214	2	-0.4552201739103552
2214	3	-0.020924684666738358
2214	4	0.38570949002660626
2214	5	-0.9031607707599463
2214	6	0.14126517631633817
2214	7	-1.28672033753125
2214	8	0.30936039212578237
2214	9	-0.15250779737190356
2214	10	-0.015500396549157358
2214	11	0.03002007599588552
2214	12	-0.2689741872479061
2214	13	0.8227643627747199
2214	14	0.4290183624929256
2214	15	-1.3396998442241213
2214	16	-1.3491636430620488
2214	17	0.4887348229484232
2214	18	0.8855520072334286
2214	19	0.7570618504614757
2214	20	0.4736970622978731
2214	21	-0.48615939503675065
2214	22	-0.4958703713050454
2214	23	-0.19569532672398537
2214	24	0.05221294276863375
2214	25	0.9406658436089117
2215	1	0.4731158358303098
2215	2	0.7823277188099489
2215	3	1.2626002195478243
2215	4	1.1328359268082195
2215	5	1.8682313109769595
2215	6	1.0735767982831348
2215	7	0.7388224466322271
2215	8	1.3259632018320389
2215	9	1.4783989714659
2215	10	1.6383948780357684
2215	11	1.2126509827647212
2215	12	0.6761088268006219
2215	13	0.7014361379982219
2215	14	1.8147144033613856
2215	15	1.205638597666045
2215	16	1.995663943144848
2215	17	1.3584666237147118
2215	18	1.7782723137468395
2215	19	1.2602546984819085
2215	20	0.8478988502927649
2215	21	1.3222697237371932
2215	22	1.288293102513606
2215	23	1.5488768316412802
2215	24	1.0649843348503722
2215	25	2.295710543481192
2216	1	0.6953380580525312
2216	2	0.39343882992106316
2216	3	1.0403779973255993
2216	4	1.355058149030441
2216	5	1.127490570236219
2216	6	1.3883916130979514
2216	7	0.9795631873729675
2216	8	1.4741113499801841
2216	9	1.7746952677621977
2216	10	1.379135618776509
2216	11	1.064502834616576
2216	12	1.194627345319141
2216	13	0.6273620639241457
2216	14	1.4443440329910118
2216	15	1.0204534124808617
2216	16	1.7178861653670694
2216	17	1.9140221792702619
2216	18	1.500494535969061
2216	19	0.7972917355189466
2216	20	-1.578027075633159
2216	21	-3.3999524984850282
2216	22	-2.193188378967882
2216	23	-4.247419464655017
2216	24	-2.7313119614459254
2216	25	-0.0746598268891745
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	2.0
4	4.0
5	2.0
6	1.0
7	2.0
8	1.0
9	0.0
10	6.0
11	8.0
12	15.0
13	25.0
14	36.0
15	48.0
16	56.0
17	63.0
18	70.0
19	59.0
20	53.0
21	67.0
22	85.0
23	88.0
24	89.0
25	112.0
26	123.0
27	114.0
28	157.0
29	186.0
30	259.0
31	294.0
32	437.0
33	566.0
34	755.0
35	1159.0
36	1801.0
37	3004.0
38	5835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62283515073765	21.00705580500321	13.867864015394485	23.502245028864657
2	14.716084275436794	21.691932168550874	40.994347379239464	22.597636176772866
3	17.95926235301677	27.044914219623468	30.566086230161282	24.429737197198484
4	11.819525676457356	17.558969085416802	39.21845876984382	31.403046468282025
5	13.094625867832347	37.65106711236822	36.05039856004114	13.203908459758292
6	31.153648509763617	37.93679342240493	18.782117163412128	12.127440904419322
7	25.364506390905007	33.014323334832035	24.490975656753804	17.130194617509154
8	22.28575099575999	37.66542464345368	21.964538095849928	18.0842862649364
9	24.017214799588903	17.68371017471737	22.186536485097637	36.1125385405961
10	14.638221308315128	29.70697853746305	33.78100501220923	21.873795142012593
11	32.19032941629744	26.00012842740641	23.784755666859308	18.024786489436845
12	21.185841844928373	27.442667180574293	31.72094815956832	19.650542814929018
13	26.151178472802005	24.46856335495472	27.96865968788132	21.411598484361953
14	21.00834938985228	22.1451509312781	28.98522800256904	27.861271676300582
15	23.14808866045615	29.662704786379695	24.343077417282363	22.846129135881785
16	22.715304090938282	30.80084772975403	26.08695652173913	20.39689165756856
17	20.582187379514203	30.70942038298419	28.723814419740396	19.984577817761213
18	20.407245632065777	29.798304213771843	29.913926002055497	19.880524152106887
19	20.537033468234085	29.151410034046382	28.96511851994604	21.346437977773494
20	22.075920097629904	29.096281071359755	28.69805382490847	20.129745006101867
21	23.18514711550816	29.956315045612232	26.455094436592574	20.403443402287035
22	20.678140251733883	29.257641921397383	29.405342923195477	20.65887490367326
23	21.87259183149242	28.814538916003084	28.30721808374005	21.00565116876445
24	20.226090307662663	28.29982657845719	28.871475367717903	22.602607746162246
25	22.133727278566383	29.121973151775965	27.702485708780266	21.041813860877383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	26.0
2	22.0
3	18.0
4	18.0
5	18.0
6	22.0
7	26.0
8	26.0
9	26.0
10	47.5
11	69.0
12	69.0
13	69.0
14	97.0
15	125.0
16	125.0
17	125.0
18	213.5
19	302.0
20	302.0
21	302.0
22	421.5
23	541.0
24	541.0
25	541.0
26	745.5
27	950.0
28	950.0
29	950.0
30	1117.5
31	1285.0
32	1285.0
33	1285.0
34	1504.5
35	1724.0
36	1724.0
37	1724.0
38	1840.5
39	1957.0
40	1957.0
41	1957.0
42	2106.0
43	2255.0
44	2255.0
45	2255.0
46	2029.0
47	1803.0
48	1803.0
49	1803.0
50	1636.5
51	1470.0
52	1470.0
53	1470.0
54	1275.5
55	1081.0
56	1081.0
57	1081.0
58	963.0
59	845.0
60	845.0
61	845.0
62	695.0
63	545.0
64	545.0
65	545.0
66	428.5
67	312.0
68	312.0
69	312.0
70	232.5
71	153.0
72	153.0
73	153.0
74	110.5
75	68.0
76	68.0
77	68.0
78	51.5
79	35.0
80	35.0
81	35.0
82	23.5
83	12.0
84	12.0
85	12.0
86	9.5
87	7.0
88	7.0
89	7.0
90	3.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12172464603754243
2	0.26266897302838105
3	0.29470177461720803
4	0.3203280158882696
5	0.33954769684156577
6	0.26266897302838105
7	0.2562624127106157
8	0.27548209366391185
9	0.26266897302838105
10	0.3011083349349734
11	0.23063617143955412
12	0.2690755333461465
13	0.24344929207508487
14	0.24985585239285027
15	0.28188865398167723
16	0.24344929207508487
17	0.3011083349349734
18	0.26266897302838105
19	0.2690755333461465
20	0.2562624127106157
21	0.27548209366391185
22	0.2370427317573195
23	0.2370427317573195
24	0.2562624127106157
25	0.2562624127106157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	15609.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.29758472676019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.07058823529412	93.45890191556153
2	1.4252100840336135	2.716381574732526
3	0.21512605042016808	0.6150297905054776
4	0.07394957983193277	0.28188865398167723
5	0.03361344537815126	0.1601640079441348
6	0.020168067226890758	0.11531808571977706
7	0.02689075630252101	0.17938368889743098
8	0.013445378151260505	0.10250496508424627
9	0.0067226890756302525	0.05765904285988853
>10	0.10756302521008404	1.8066500096098406
>50	0.0067226890756302525	0.5061182651034659
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	79	0.5061182651034659	No Hit
GCTTATGTACTCTGCGTTGATACCA	43	0.27548209366391185	No Hit
CTTATGTACTCTGCGTTGATACCAC	25	0.1601640079441348	No Hit
ATTCCATTCCATTCCATTCCATTCC	24	0.1537574476263694	No Hit
TATGTACTCTGCGTTGATACCACTG	20	0.12813120635530784	No Hit
GAGTACATAAGCAGTGGTATCAACG	20	0.12813120635530784	No Hit
GAATGGAATGGAATGGAATGGAATG	20	0.12813120635530784	No Hit
GTGGTATCAACGCAGAGTACATGGG	17	0.10891152540201167	No Hit
ACTCTGCGTTGATACCACTGCTTAT	16	0.10250496508424627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE