FastQCFastQC Report
Wed 25 May 2016
SRR1295222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8646
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN160.18505667360629194No Hit
GTGGTATCAACGCAGAGTACATGGG140.16192458940550544No Hit
ATTCCATTCCATTCCATTCCATTCC110.1272264631043257No Hit
GGTATCAACGCAGAGTACTTTTTTT100.11566042100393245No Hit
GTATCAACGCAGAGTACTTTTTTTT90.1040943789035392No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)