FastQCFastQC Report
Wed 25 May 2016
SRR1295222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8646
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT380.43950959981494336No Hit
TATCAACGCAGAGTACTTTTTTTTT270.31228313671061764No Hit
GGTATCAACGCAGAGTACTTTTTTT170.19662271570668519No Hit
GTGGTATCAACGCAGAGTACATGGG150.17349063150589866No Hit
ACGCAGAGTACTTTTTTTTTTTTTT120.13879250520471895No Hit
GAGTACTTTTTTTTTTTTTTTTTTT100.11566042100393245No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)