##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295222_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8646 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.425630349294472 31.0 31.0 33.0 28.0 34.0 2 31.461022438121674 31.0 31.0 34.0 28.0 34.0 3 31.649895905621097 31.0 31.0 34.0 29.0 34.0 4 35.254799907471664 37.0 35.0 37.0 33.0 37.0 5 35.36271108026833 37.0 35.0 37.0 33.0 37.0 6 35.220101781170484 37.0 35.0 37.0 33.0 37.0 7 35.17210270645385 37.0 35.0 37.0 32.0 37.0 8 35.11820495026602 37.0 35.0 37.0 32.0 37.0 9 36.885380522785105 39.0 37.0 39.0 33.0 39.0 10 36.87196391394865 39.0 37.0 39.0 33.0 39.0 11 36.93916261855193 39.0 37.0 39.0 33.0 39.0 12 36.8587786259542 39.0 37.0 39.0 33.0 39.0 13 36.77295859356928 39.0 37.0 39.0 32.0 39.0 14 38.00763358778626 40.0 38.0 41.0 33.0 41.0 15 37.98126301179736 40.0 38.0 41.0 32.0 41.0 16 38.004279435577146 40.0 38.0 41.0 33.0 41.0 17 37.974554707379134 40.0 38.0 41.0 33.0 41.0 18 38.01885264862364 40.0 38.0 41.0 33.0 41.0 19 38.09819569743234 40.0 38.0 41.0 33.0 41.0 20 38.113462873004856 40.0 38.0 41.0 33.0 41.0 21 38.06349757113116 40.0 38.0 41.0 33.0 41.0 22 38.08547305112191 40.0 38.0 41.0 33.0 41.0 23 37.987508674531576 40.0 38.0 41.0 32.0 41.0 24 37.95616470043951 40.0 38.0 41.0 32.0 41.0 25 36.95697432338654 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09674494506426967 1101 2 -1.8190482421925047 1101 3 0.11806240237613608 1101 4 -0.6427155399844366 1101 5 -1.5673844311099288 1101 6 -0.2491956184183124 1101 7 0.1712868143890489 1101 8 0.5233220884831411 1101 9 0.526231413311244 1101 10 -0.5440143177188048 1101 11 -0.06610645941876925 1101 12 -0.005722057017578663 1101 13 -0.09491666907632634 1101 14 -1.15407863222989 1101 15 -1.0649979909083669 1101 16 0.017966802105455315 1101 17 0.848572191114215 1101 18 -0.6473607054839334 1101 19 -0.5441448944249956 1101 20 0.1627134161097743 1101 21 -0.5057719707929849 1101 22 0.48007111417476267 1101 23 -1.7068817477781053 1101 24 0.30003666061089973 1101 25 -0.16732461179240232 1102 1 0.7634116117309375 1102 2 0.7476184244741617 1102 3 1.218062402376134 1102 4 0.4906177933488962 1102 5 0.36594890222340126 1102 6 1.0508043815816919 1102 7 0.9546201477223875 1102 8 1.1733220884831397 1102 9 1.2595647466445783 1102 10 1.2559856822811923 1102 11 1.0172268739145665 1102 12 1.2776112763157599 1102 13 1.755083330923675 1102 14 1.0125880344367744 1102 15 1.8016686757583003 1102 16 -0.015366531227876123 1102 17 1.2985721911142107 1102 18 1.0359726278493966 1102 19 -0.34414489442499274 1102 20 -0.6039532505568914 1102 21 -0.13910530412631772 1102 22 -1.2532622191585716 1102 23 -1.5568817477781067 1102 24 -0.09996333938909885 1102 25 -0.250657945125738 1103 1 0.28722113554045947 1103 2 -0.22381014695440982 1103 3 -9.852166714807709E-4 1103 4 0.833474936206045 1103 5 0.21832985460435594 1103 6 0.369852000629308 1103 7 -1.0310941379919 1103 8 -0.083820768659713 1103 9 1.121469508549339 1103 10 0.8369380632335748 1103 11 0.23151258820028175 1103 12 1.077611276315757 1103 13 0.29794047378081956 1103 14 0.44115946300820497 1103 15 1.6492877233773484 1103 16 0.7798715640102216 1103 17 -0.1633325707905513 1103 18 0.26930596118273087 1103 19 -0.13938298966309048 1103 20 -1.6230008696045104 1103 21 -0.26767673269775116 1103 22 -0.5913574572538067 1103 23 -0.1473579382542951 1103 24 0.9190842796585201 1103 25 -0.10780080226859212 1104 1 -0.6468447985254748 1104 2 -1.4985354216796836 1104 3 -2.4075786232648895 1104 4 -0.38630528357418115 1104 5 -0.028922892648388654 1104 6 -1.10816997739267 1104 7 -0.3992260061237687 1104 8 -0.765139449978399 1104 9 -2.1917173046374785 1104 10 -1.646578420282907 1104 11 -1.675080818393127 1104 12 0.23145743016191034 1104 13 -0.9987628229224796 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0.8319055244475493 2211 18 0.5026392945160651 2211 19 0.989188438908343 2211 20 0.99604674944311 2211 21 0.9275613625403452 2211 22 -0.45326221915856735 2211 23 -0.22354841444477103 2211 24 -0.16663000605576173 2211 25 -2.117324611792405 2212 1 0.4300782783976018 2212 2 0.20476128161702079 2212 3 -0.5724137881000537 2212 4 -0.8808107780796703 2212 5 0.8611869974615018 2212 6 0.7984234292007386 2212 7 0.18319157629381522 2212 8 -0.012392197231143598 2212 9 -0.09281620573637639 2212 10 0.3369380632335748 2212 11 -0.9113445546568641 2212 12 0.577611276315757 2212 13 1.0122261880665278 2212 14 -0.6302691084203715 2212 15 -0.4935694194797975 2212 16 -1.148699864561209 2212 17 -0.1633325707905513 2212 18 -0.9449797531029844 2212 19 -0.21081156109165988 2212 20 -1.1230008696045104 2212 21 -2.41053387555489 2212 22 0.8372139713176168 2212 23 1.066927776031413 2212 24 1.061941422515666 2212 25 0.24934205487426198 2213 1 0.6443639926833171 2213 2 0.7047612816170172 2213 3 0.5704433547570886 2213 4 0.47633207906318376 2213 5 0.5754727117472171 2213 6 0.5841377149150233 2213 7 0.6831915762938152 2213 8 1.3447506599117105 2213 9 1.0500409371207624 2213 10 0.9083666346621442 2213 11 1.0886554453431359 2213 12 1.577611276315757 2213 13 1.1550833309236737 2213 14 0.7983023201510591 2213 15 1.077859151948779 2213 16 1.5655858497245063 2213 17 1.550953143495164 2213 18 0.626448818325585 2213 19 1.5034741531940554 2213 20 1.0198562732526284 2213 21 0.6608946958736794 2213 22 0.12292825703190857 2213 23 0.3526420617457049 2213 24 1.2047985653728048 2213 25 1.4636277691599773 2214 1 0.8050782783976018 2214 2 0.34761842447416313 2214 3 -0.40277093095719607 2214 4 0.12811779334889906 2214 5 0.43261556889007124 2214 6 0.7091377149150233 2214 7 -0.7453798522776154 2214 8 -0.1016779115168589 2214 9 0.5678980799779083 2214 10 0.4351523489478595 2214 11 1.0797268739145665 2214 12 -1.047388723684243 2214 13 0.6550833309236737 2214 14 0.26258803443677436 2214 15 0.1850020090916331 2214 16 0.10130013543879102 2214 17 0.6402388577808793 2214 18 0.26930596118273087 2214 19 -2.64831156109166 2214 20 -0.6497865838902257 2214 21 0.41089469587367944 2214 22 -0.7699288858252373 2214 23 -0.04021508111144101 2214 24 1.1333699939442354 2214 25 -0.18815794512573802 2215 1 -0.10325505493573317 2215 2 -0.519048242192504 2215 3 0.28472906904280393 2215 4 -1.176048873317768 2215 5 0.2992822355567384 2215 6 -0.21586228508497385 2215 7 0.4879534810557189 2215 8 0.1066554218164768 2215 9 -1.6071019200220888 2215 10 -1.5440143177188048 2215 11 -1.449439792752102 2215 12 -1.3890553903509115 2215 13 -1.178250002409662 2215 14 -2.1874119655632285 2215 15 -0.931664657575034 2215 16 -0.5486998645612076 2215 17 -0.16809447555245072 2215 18 0.16930596118272945 2215 19 -0.07747822775832702 2215 20 0.2627134161097757 2215 21 -0.33910530412632056 2215 22 0.2800711141747598 2215 23 0.24311825222189043 2215 24 0.6333699939442354 2215 25 -0.250657945125738 2216 1 0.09674494506426967 2216 2 1.2365073133630489 2216 3 1.1736179579316897 2216 4 1.0239511266822348 2216 5 1.2103933466678498 2216 6 -0.4158622850849767 2216 7 0.8657312588334989 2216 8 1.217766532927584 2216 9 0.526231413311244 2216 10 1.2337634600589737 2216 11 0.2394490961367879 2216 12 0.6331668318713142 2216 13 1.4884166642570094 2216 14 0.6792547011034387 2216 15 1.0461131202027474 2216 16 0.18463346877212672 2216 17 1.4319055244475436 2216 18 1.324861516738288 2216 19 0.5669662166861187 2216 20 0.7738245272208886 2216 21 0.9942280292070151 2216 22 0.3689600030636484 2216 23 1.5986738077774447 2216 24 1.5222588828331212 2216 25 2.9715642770964834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 5.0 12 3.0 13 5.0 14 11.0 15 12.0 16 19.0 17 14.0 18 22.0 19 27.0 20 27.0 21 24.0 22 31.0 23 32.0 24 27.0 25 38.0 26 43.0 27 44.0 28 70.0 29 63.0 30 105.0 31 127.0 32 185.0 33 292.0 34 385.0 35 576.0 36 822.0 37 1567.0 38 4068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55146888734675 20.99236641221374 12.68794818413139 23.76821651630812 2 15.706685172334028 21.547536433032615 38.73467499421698 24.011103400416378 3 17.941006362058996 25.841526894158473 30.121457489878544 26.09600925390399 4 12.5028915105251 17.77700670830442 38.225769141799674 31.49433263937081 5 14.214665741383298 37.09229701596114 34.628730048577374 14.064307194078188 6 31.297709923664126 38.88503354152209 17.233402729585936 12.583853805227852 7 25.503122831367108 32.84755956511682 23.930141105713627 17.71917649780245 8 23.617857969003005 37.612768910478835 20.84200786490863 17.92736525560953 9 24.404348831829747 16.863289382373353 21.69789498033773 37.03446680545917 10 14.86236409900532 28.868841082581543 34.21235253296322 22.056442285449922 11 31.5058986814712 24.84385843164469 24.392782789729356 19.257460097154755 12 20.821283979178716 27.79641411220359 31.116252168883747 20.266049739733948 13 26.469227209625174 23.900971772327626 26.469227209625174 23.16057380842203 14 20.217441591487393 23.467499421697894 28.32523710386306 27.989821882951656 15 23.490631505898683 30.268332176729125 23.918575063613233 22.322461253758963 16 21.975479990747164 30.765671987046034 26.01202868378441 21.246819338422394 17 20.495026601896832 29.666897987508673 28.498727735368956 21.339347675225536 18 19.96298866527874 29.19269026139255 29.875086745315755 20.969234328012952 19 21.20055517002082 28.61438815637289 30.002313208420077 20.182743465186213 20 22.148970622253064 28.117048346055977 29.72472819801064 20.009252833680314 21 23.54846171640065 28.128614388156375 28.30210501966227 20.02081887578071 22 21.721027064538514 27.654406662040248 30.383992597733055 20.24057367568818 23 21.640064769835764 27.989821882951656 29.747860282211423 20.622253065001157 24 20.934536201711772 28.117048346055977 29.019199629886653 21.929215822345594 25 21.76729123294009 27.851029377746933 28.521859819569745 21.859819569743234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 7.0 2 6.0 3 5.0 4 5.0 5 5.0 6 7.5 7 10.0 8 10.0 9 10.0 10 22.5 11 35.0 12 35.0 13 35.0 14 55.0 15 75.0 16 75.0 17 75.0 18 118.0 19 161.0 20 161.0 21 161.0 22 240.0 23 319.0 24 319.0 25 319.0 26 412.0 27 505.0 28 505.0 29 505.0 30 607.0 31 709.0 32 709.0 33 709.0 34 809.5 35 910.0 36 910.0 37 910.0 38 998.5 39 1087.0 40 1087.0 41 1087.0 42 1066.0 43 1045.0 44 1045.0 45 1045.0 46 1011.5 47 978.0 48 978.0 49 978.0 50 905.5 51 833.0 52 833.0 53 833.0 54 759.5 55 686.0 56 686.0 57 686.0 58 602.5 59 519.0 60 519.0 61 519.0 62 432.0 63 345.0 64 345.0 65 345.0 66 272.0 67 199.0 68 199.0 69 199.0 70 160.5 71 122.0 72 122.0 73 122.0 74 89.5 75 57.0 76 57.0 77 57.0 78 39.0 79 21.0 80 21.0 81 21.0 82 16.0 83 11.0 84 11.0 85 11.0 86 8.0 87 5.0 88 5.0 89 5.0 90 3.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.011566042100393246 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.011566042100393246 13 0.023132084200786492 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 8646.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.54175341198243 #Duplication Level Percentage of deduplicated Percentage of total 1 98.22690787109141 94.82997918112423 2 1.4855636755720618 2.868378440897525 3 0.07188211333413203 0.2081887578070784 4 0.04792140888942135 0.18505667360629194 5 0.07188211333413203 0.3469812630117973 6 0.0 0.0 7 0.0 0.0 8 0.023960704444710674 0.18505667360629194 9 0.0 0.0 >10 0.07188211333413203 1.3763590099467962 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 38 0.43950959981494336 No Hit TATCAACGCAGAGTACTTTTTTTTT 27 0.31228313671061764 No Hit GGTATCAACGCAGAGTACTTTTTTT 17 0.19662271570668519 No Hit GTGGTATCAACGCAGAGTACATGGG 15 0.17349063150589866 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12 0.13879250520471895 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 10 0.11566042100393245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE