FastQCFastQC Report
Wed 25 May 2016
SRR1295221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA3917.549720023170496No Hit
GCTTATGTACTCTGCGTTGATACCA2705.213361652828731No Hit
CTTATGTACTCTGCGTTGATACCAC1763.3983394477698394No Hit
TATGTACTCTGCGTTGATACCACTG1011.9501834330951922No Hit
GAGTACATAAGCAGTGGTATCAACG961.853639698783549No Hit
GCGTTGATACCACTGCTTATGTACT881.6991697238849197No Hit
CATAAGCAGTGGTATCAACGCAGAG701.3516122803630044No Hit
GTATCAACGCAGAGTACATAAGCAG671.2936860397760186No Hit
CCCATGTACTCTGCGTTGATACCAC671.2936860397760186No Hit
ACTCTGCGTTGATACCACTGCTTAT601.1585248117397182No Hit
ATCATGTACTCTGCGTTGATACCAC551.061981077428075No Hit
CATGTACTCTGCGTTGATACCACTG521.004054836841089No Hit
ACGCAGAGTACATAAGCAGTGGTAT490.9461285962541031No Hit
GTACATGATAAGCAGTGGTATCAAC470.9075111025294458No Hit
TATCAACGCAGAGTACATAAGCAGT450.8688936088047886No Hit
CTGCTTATGTACTCTGCGTTGATAC440.8495848619424599No Hit
AAGCAGTGGTATCAACGCAGAGTAC340.6564973933191736No Hit
GTGGTATCAACGCAGAGTACATAAG320.6178798995945163No Hit
GGTATCAACGCAGAGTACATAAGCA310.5985711527321876No Hit
GTACTCTGCGTTGATACCACTGCTT300.5792624058698591No Hit
GCAGTGGTATCAACGCAGAGTACAT300.5792624058698591No Hit
GTACATGGGAAGCAGTGGTATCAAC270.5213361652828732No Hit
GAGTACATGATAAGCAGTGGTATCA250.48271867155821585No Hit
TATCAACGCAGAGTACATGATAAGC210.40548368410890134No Hit
CCACTGCTTATGTACTCTGCGTTGA210.40548368410890134No Hit
GTGGTATCAACGCAGAGTACATGGG200.3861749372465727No Hit
GCTTATCATGTACTCTGCGTTGATA200.3861749372465727No Hit
ATACCACTGCTTATGTACTCTGCGT190.36686619038424406No Hit
GTTGATACCACTGCTTATGTACTCT190.36686619038424406No Hit
GCTTATCCCATGTACTCTGCGTTGA190.36686619038424406No Hit
ACATAAGCAGTGGTATCAACGCAGA180.3475574435219154No Hit
GATACCACTGCTTATGTACTCTGCG180.3475574435219154No Hit
GCGTTGATACCACTGCTTATCATGT170.3282486966595868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA170.3282486966595868No Hit
CAGTGGTATCAACGCAGAGTACATA150.28963120293492955No Hit
GCAGAGTACATAAGCAGTGGTATCA150.28963120293492955No Hit
GGATAAGCAGTGGTATCAACGCAGA150.28963120293492955No Hit
TGATACCACTGCTTATGTACTCTGC140.27032245607260086No Hit
GTACATGGGATAAGCAGTGGTATCA140.27032245607260086No Hit
CCCCATGTACTCTGCGTTGATACCA130.2510137092102723No Hit
CTGCTTATCATGTACTCTGCGTTGA130.2510137092102723No Hit
GATAAGCAGTGGTATCAACGCAGAG130.2510137092102723No Hit
CATGTAAGCAGTGGTATCAACGCAG130.2510137092102723No Hit
GGTATCAACGCAGAGTACATGATAA130.2510137092102723No Hit
GCGTTGATACCACTGCTTCCCATGT130.2510137092102723No Hit
GAGTACATGGGAAGCAGTGGTATCA130.2510137092102723No Hit
GCGTTGATACCACTGCTTATCCCAT120.23170496234794363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT120.23170496234794363No Hit
GCTTACATGTACTCTGCGTTGATAC120.23170496234794363No Hit
CAGTGGTATCAACGCAGAGTACATG110.21239621548561496No Hit
TACCACTGCTTATCATGTACTCTGC110.21239621548561496No Hit
GAACAAAAAAAAAAAAAAAAAAAAA110.21239621548561496No Hit
GTACATGGTAAGCAGTGGTATCAAC100.19308746862328635No Hit
GTACATGTAAGCAGTGGTATCAACG100.19308746862328635No Hit
ATCAACGCAGAGTACATAAGCAGTG100.19308746862328635No Hit
AAAAAGTACTCTGCGTTGATACCAC100.19308746862328635No Hit
ACGCAGAGTACATGATAAGCAGTGG100.19308746862328635No Hit
GTATCAACGCAGAGTACATGATAAG100.19308746862328635No Hit
GGTATCAACGCAGAGTACTTTTTTT100.19308746862328635No Hit
GTTGATACCACTGCTTATCATGTAC90.1737787217609577No Hit
TACCACTGCTTATGTACTCTGCGTT90.1737787217609577No Hit
ACATGATAAGCAGTGGTATCAACGC90.1737787217609577No Hit
CATGATAAGCAGTGGTATCAACGCA90.1737787217609577No Hit
GTATCAACGCAGAGTACATGGGAAG90.1737787217609577No Hit
GTATCAACGCAGAGTACTTTTTTTT90.1737787217609577No Hit
ACGCAGAGTACATGGGATAAGCAGT80.15446997489862907No Hit
GTACTGGTTCACTATCGGTCAGTCA80.15446997489862907No Hit
GGTATCAACGCAGAGTACATGGGAT80.15446997489862907No Hit
GAGTACATGTAAGCAGTGGTATCAA70.13516122803630043No Hit
ACCATGTACTCTGCGTTGATACCAC70.13516122803630043No Hit
GATACCACTGCTTATCATGTACTCT70.13516122803630043No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA70.13516122803630043No Hit
GAGTACATGGGATAAGCAGTGGTAT70.13516122803630043No Hit
ACGCAGAGTACATGGGAAGCAGTGG70.13516122803630043No Hit
CTGCGTTGATACCACTGCTTATGTA70.13516122803630043No Hit
CCATGTACTCTGCGTTGATACCACT70.13516122803630043No Hit
ATGTACTCTGCGTTGATACCACTGC60.11585248117397182No Hit
TGATAAGCAGTGGTATCAACGCAGA60.11585248117397182No Hit
ACTCTGCGTTGATACCACTGCTTCC60.11585248117397182No Hit
ATGATAAGCAGTGGTATCAACGCAG60.11585248117397182No Hit
TATCAACGCAGAGTACATGGGATAA60.11585248117397182No Hit
GGATACCACGTGTCCCGCCCTACTC60.11585248117397182No Hit
TATCAACGCAGAGTACTTTTTTTTT60.11585248117397182No Hit
AAAGTACTCTGCGTTGATACCACTG60.11585248117397182No Hit
GCTTACCATGTACTCTGCGTTGATA60.11585248117397182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG755.405236E-713.9333321
GTACATA852.2268996E-612.2941181
TACATAA852.2268996E-612.2941182
ACATAAG852.2268996E-612.2941183
TGCGTTG1154.0610757E-710.7391313
CTGCGTT1154.0610757E-710.7391312
CTTATGT1001.3757508E-510.452
CATAAGC1001.3757508E-510.454
TTATGTA1001.3757508E-510.453
CTCTGCG1207.0608075E-710.29166710
GTACTCT1207.0608075E-710.2916677
TCTGCGT1251.1981138E-69.8811
GCGTTGA1251.1981138E-69.8814
CGTTGAT1251.1981138E-69.8815
TACTCTG1251.1981138E-69.888
GTTGATA1301.9879317E-69.516
ATAAGCA1103.961068E-59.55
TAAGCAG1103.961068E-59.56
TATGTAC1103.961068E-59.54
ACTCTGC1301.9879317E-69.59
Produced by FastQC (version 0.11.5)