##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295221_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5179
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.140953852095	31.0	30.0	33.0	26.0	34.0
2	30.158524811739717	31.0	30.0	34.0	26.0	34.0
3	30.338675419965245	31.0	30.0	34.0	26.0	34.0
4	33.520370727939756	35.0	35.0	37.0	27.0	37.0
5	33.924309712299674	35.0	35.0	37.0	28.0	37.0
6	34.11314925661325	37.0	35.0	37.0	30.0	37.0
7	34.17570959644719	37.0	35.0	37.0	30.0	37.0
8	34.00038617493725	37.0	35.0	37.0	29.0	37.0
9	35.0986676964665	39.0	35.0	39.0	27.0	39.0
10	35.142498551843985	39.0	35.0	39.0	27.0	39.0
11	35.56555319559761	39.0	35.0	39.0	28.0	39.0
12	35.60919096350647	39.0	35.0	39.0	29.0	39.0
13	35.33519984553003	39.0	35.0	39.0	27.0	39.0
14	36.10252944583897	39.0	36.0	41.0	27.0	41.0
15	36.345626568835684	39.0	36.0	41.0	27.0	41.0
16	36.03012164510523	39.0	36.0	41.0	27.0	41.0
17	36.06815987642402	39.0	36.0	41.0	27.0	41.0
18	36.05696080324387	39.0	36.0	41.0	27.0	41.0
19	36.497586406642206	39.0	36.0	41.0	28.0	41.0
20	36.55647808457231	40.0	36.0	41.0	28.0	41.0
21	36.60668082641437	40.0	36.0	41.0	28.0	41.0
22	36.50106198107743	40.0	36.0	41.0	28.0	41.0
23	36.19308746862329	39.0	36.0	41.0	27.0	41.0
24	36.453562463796096	40.0	36.0	41.0	27.0	41.0
25	35.33944776983974	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6436822877299164
1101	2	0.9131897203325714
1101	3	0.5908163265306108
1101	4	0.6447782816830383
1101	5	2.015803728898973
1101	6	1.0763542454018662
1101	7	-1.621409674981102
1101	8	0.7932854623330883
1101	9	-1.363227513227514
1101	10	0.11066389518770592
1101	11	-0.24783320735701864
1101	12	1.0297241118669689
1101	13	0.17897455278406937
1101	14	1.3110607205845355
1101	15	0.348481985386762
1101	16	2.2087994457042157
1101	17	2.8757306626354193
1101	18	-0.45803099017384596
1101	19	1.3123771730914555
1101	20	1.2937893675988832
1101	21	-0.764430587049624
1101	22	2.2938586545729507
1101	23	1.968398841017894
1101	24	-0.2812043335852863
1101	25	0.1584467120181472
1102	1	-0.7313177122700836
1102	2	-0.30903250188965004
1102	3	-0.1730725623582785
1102	4	-0.1885550516502974
1102	5	-1.970307382212141
1102	6	-2.3958679768203552
1102	7	0.10081254724111943
1102	8	-3.3317145376669117
1102	9	-1.238227513227514
1102	10	0.45788611740992735
1102	11	-1.0533887629125758
1102	12	0.15472411186696888
1102	13	-2.2654698916603735
1102	14	-2.536161501637686
1102	15	-2.4292957923910166
1102	16	-1.9023116654068986
1102	17	-3.485380448475695
1102	18	-0.7496976568405103
1102	19	-2.1737339380196588
1102	20	-0.8312106324011168
1102	21	0.7494583018392618
1102	22	-2.8172524565381636
1102	23	0.3989543965734512
1102	24	-1.364537666918622
1102	25	-1.89710884353741
1103	1	-0.8007621567145264
1103	2	-0.8645880574452072
1103	3	-0.07585034013605707
1103	4	0.8670005039052597
1103	5	-0.3175296044343625
1103	6	-1.8125346434870195
1103	7	-0.8436318972033234
1103	8	-1.5400478710002474
1103	9	1.3034391534391503
1103	10	0.9995527840765916
1103	11	1.1966112370874242
1103	12	0.14083522297808315
1103	13	-0.5988032249937092
1103	14	-0.8000503905265717
1103	15	1.4595930964978692
1103	16	-2.013422776518013
1103	17	-0.6798248929201378
1103	18	-1.3469198790627317
1103	19	0.3123771730914555
1103	20	-1.0395439657344525
1103	21	-1.208875031494074
1103	22	-0.4839191232048279
1103	23	-4.142712270093213
1103	24	-2.5034265558075077
1103	25	-2.952664399092967
1104	1	-1.8563177122700836
1104	2	-0.55903250188965
1104	3	-1.0480725623582785
1104	4	-4.563555051650297
1104	5	-2.220307382212141
1104	6	-7.395867976820355
1104	7	-4.399187452758881
1104	8	-2.5817145376669117
1104	9	-8.863227513227514
1104	10	-7.667113882590073
1104	11	-3.4283887629125758
1104	12	-5.345275888133031
1104	13	-5.6404698916603735
1104	14	-8.286161501637686
1104	15	-5.929295792391017
1104	16	-6.7773116654068986
1104	17	-2.110380448475695
1104	18	-8.74969765684051
1104	19	-3.9237339380196588
1104	20	-5.081210632401117
1104	21	-3.8755416981607382
1104	22	-5.817252456538164
1104	23	-3.476045603426549
1104	24	-4.489537666918622
1104	25	-2.27210884353741
1105	1	0.7865394305870588
1105	2	-1.30903250188965
1105	3	0.5590702947845791
1105	4	-4.25998362307887
1105	5	1.1904069035021436
1105	6	-0.5744394053917858
1105	7	1.6008125472411194
1105	8	0.7932854623330883
1105	9	-2.6489417989417987
1105	10	0.761457545981358
1105	11	-2.2319601914840064
1105	12	0.886866969009823
1105	13	-0.9797556059460888
1105	14	1.23169564121946
1105	15	2.142132779037553
1105	16	0.526259763164532
1105	17	-1.5210947341899796
1105	18	1.7324452003023438
1105	19	-1.3701625094482281
1105	20	0.00807508188459849
1105	21	-1.0183988410178841
1105	22	-0.6743953136810177
1105	23	-1.6189027462836947
1105	24	0.0997480473670933
1105	25	-0.8256802721088405
1106	1	-0.07060342655579888
1106	2	0.8338246409674923
1106	3	-2.7266439909297056
1106	4	0.0257306626354179
1106	5	0.19040690350214362
1106	6	-0.28872511967750114
1106	7	0.45795540438397353
1106	8	-1.6352859662383423
1106	9	-2.50608465608466
1106	10	-1.8099710254472186
1106	11	0.33946837994457013
1106	12	0.6011526832955383
1106	13	1.5916729654824806
1106	14	0.9459813555051682
1106	15	-3.2864386495338707
1106	16	0.6691169060216708
1106	17	1.3360481229528745
1106	18	1.3038737717309203
1106	19	1.6298374905517719
1106	20	1.8652179390274526
1106	21	1.6958868732678312
1106	22	1.4684618291761211
1106	23	1.3810972537163053
1106	24	1.0997480473670933
1106	25	1.3171768707483054
1107	1	-0.8007621567145264
1107	2	0.69096749811035
1107	3	1.1463718820861644
1107	4	1.0892227261274812
1107	5	0.23802595112119462
1107	6	1.6319098009574233
1107	7	1.4897014361300052
1107	8	1.682174351221974
1107	9	3.192328042328043
1107	10	2.8884416729654845
1107	11	2.1966112370874242
1107	12	1.3630574452003046
1107	13	1.8456412194507408
1107	14	3.533282942806757
1107	15	2.5707042076089834
1107	16	3.319910556815323
1107	17	3.209063995968748
1107	18	2.430857898715047
1107	19	1.4234882842025627
1107	20	-1.817321743512231
1107	21	1.3466805240614832
1107	22	2.071636432350722
1107	23	2.412843285462337
1107	24	2.4965734441924923
1107	25	3.9362244897959258
1108	1	-3.456317712270085
1108	2	-0.40903250188965146
1108	3	-2.89807256235828
1108	4	-7.488555051650298
1108	5	-3.6953073822121425
1108	6	-3.8458679768203545
1108	7	-1.2991874527588791
1108	8	-2.6067145376669103
1108	9	-2.363227513227514
1108	10	-6.267113882590074
1108	11	-3.5033887629125786
1108	12	-6.5702758881330325
1108	13	-1.7654698916603735
1108	14	-6.011161501637687
1108	15	-6.029295792391018
1108	16	-2.7023116654068957
1108	17	-3.535380448475692
1108	18	-6.3246976568405096
1108	19	-5.998733938019658
1108	20	-5.606210632401115
1108	21	-8.47554169816074
1108	22	-4.717252456538162
1108	23	-4.276045603426546
1108	24	-7.514537666918621
1108	25	-6.89710884353741
1109	1	-1.8563177122700836
1109	2	1.19096749811035
1109	3	-0.9230725623582785
1109	4	1.0614449483497026
1109	5	-1.595307382212141
1109	6	-1.2708679768203552
1109	7	-4.024187452758881
1109	8	-2.0817145376669117
1109	9	-1.238227513227514
1109	10	-2.4171138825900726
1109	11	-3.0533887629125758
1109	12	-3.595275888133031
1109	13	-3.0154698916603735
1109	14	-3.536161501637686
1109	15	-4.554295792391017
1109	16	-2.4023116654068986
1109	17	-0.48538044847569495
1109	18	-4.74969765684051
1109	19	-4.298733938019659
1109	20	-1.2062106324011168
1109	21	-0.12554169816073824
1109	22	-3.3172524565381636
1109	23	-3.351045603426549
1109	24	-4.114537666918622
1109	25	-1.39710884353741
1110	1	1.7686822877299164
1110	2	1.56596749811035
1110	3	1.3269274376417215
1110	4	2.0614449483497026
1110	5	1.404692617787859
1110	6	2.1041320231796448
1110	7	2.1008125472411194
1110	8	1.4182854623330883
1110	9	0.386772486772486
1110	10	1.7078861174099274
1110	11	1.6966112370874242
1110	12	0.9047241118669689
1110	13	1.7345301083396265
1110	14	0.21383849836231406
1110	15	-0.6792957923910166
1110	16	1.8476883345931014
1110	17	-0.36038044847569495
1110	18	-0.37469765684051026
1110	19	2.5762660619803412
1110	20	1.2937893675988832
1110	21	2.1244583018392618
1110	22	0.3077475434618364
1110	23	1.7739543965734512
1110	24	2.385462333081378
1110	25	1.72789115646259
1111	1	-0.23131771227008358
1111	2	0.31596749811034996
1111	3	-1.2980725623582785
1111	4	-0.1885550516502974
1111	5	-0.8453073822121411
1111	6	1.4791320231796448
1111	7	0.6008125472411194
1111	8	0.6682854623330883
1111	9	1.011772486772486
1111	10	-0.9171138825900726
1111	11	-1.9283887629125758
1111	12	-1.5952758881330311
1111	13	-0.8904698916603735
1111	14	0.33883849836231406
1111	15	-1.0542957923910166
1111	16	1.8476883345931014
1111	17	0.13961955152430505
1111	18	-0.37469765684051026
1111	19	0.32626606198034125
1111	20	-1.5812106324011168
1111	21	-2.5005416981607382
1111	22	-1.1922524565381636
1111	23	-1.8510456034265488
1111	24	-0.364537666918622
1111	25	-1.52210884353741
1112	1	-0.6063177122700836
1112	2	1.06596749811035
1112	3	0.7019274376417215
1112	4	2.1864449483497026
1112	5	1.404692617787859
1112	6	0.35413202317964476
1112	7	0.7258125472411194
1112	8	1.4182854623330883
1112	9	-2.738227513227514
1112	10	0.7078861174099274
1112	11	1.0716112370874242
1112	12	1.2797241118669689
1112	13	-1.0154698916603735
1112	14	0.9638384983623141
1112	15	-0.17929579239101656
1112	16	-0.27731166540689856
1112	17	-0.985380448475695
1112	18	-0.24969765684051026
1112	19	-0.17373393801965875
1112	20	2.043789367598883
1112	21	1.7494583018392618
1112	22	1.5577475434618364
1112	23	0.2739543965734512
1112	24	1.635462333081378
1112	25	2.85289115646259
1113	1	0.3936822877299164
1113	2	0.44096749811034996
1113	3	0.5769274376417215
1113	4	-0.6885550516502974
1113	5	0.2796926177878589
1113	6	0.8541320231796448
1113	7	-0.024187452758880568
1113	8	1.4182854623330883
1113	9	1.886772486772486
1113	10	0.9578861174099274
1113	11	-0.5533887629125758
1113	12	0.029724111866968883
1113	13	-1.3904698916603735
1113	14	-1.911161501637686
1113	15	0.32070420760898344
1113	16	-1.7773116654068986
1113	17	-1.235380448475695
1113	18	-1.3746976568405103
1113	19	-4.798733938019659
1113	20	-3.081210632401117
1113	21	-0.37554169816073824
1113	22	-0.9422524565381636
1113	23	-0.9760456034265488
1113	24	-0.239537666918622
1113	25	-1.02210884353741
1114	1	-1.0706034265557989
1114	2	1.69096749811035
1114	3	-0.15521541950113615
1114	4	1.1685878054925567
1114	5	1.6189783320735742
1114	6	1.7112748803224989
1114	7	-0.8277588813303112
1114	8	2.221856890904519
1114	9	2.636772486772486
1114	10	1.4757432602670733
1114	11	2.0537540942302783
1114	12	0.7440098261526842
1114	13	1.5916729654824806
1114	14	2.8031242126480294
1114	15	2.999275636180414
1114	16	3.383402620307386
1114	17	2.907476694381451
1114	18	3.3038737717309203
1114	19	0.9155517762660565
1114	20	1.2937893675988832
1114	21	0.8387440161249771
1114	22	2.1827475434618364
1114	23	2.952525825144882
1114	24	1.9568909045099474
1114	25	2.3171768707483054
1115	1	-0.8563177122700836
1115	2	-1.55903250188965
1115	3	-0.4230725623582785
1115	4	-1.5635550516502974
1115	5	-0.3453073822121411
1115	6	-4.270867976820355
1115	7	-2.7741874527588806
1115	8	-3.7067145376669117
1115	9	-4.613227513227514
1115	10	-5.167113882590073
1115	11	-1.6783887629125758
1115	12	-5.595275888133031
1115	13	-3.2654698916603735
1115	14	-4.661161501637686
1115	15	-6.054295792391017
1115	16	-4.1523116654068986
1115	17	-3.110380448475695
1115	18	-6.49969765684051
1115	19	-4.423733938019659
1115	20	-4.831210632401117
1115	21	-4.500541698160738
1115	22	-7.192252456538164
1115	23	-4.601045603426549
1115	24	-4.739537666918622
1115	25	-1.39710884353741
1116	1	1.1992378432854736
1116	2	0.024300831443682114
1116	3	2.035260770975057
1116	4	0.3114449483497026
1116	5	0.23802595112119462
1116	6	1.409687578735202
1116	7	1.4897014361300052
1116	8	-1.0956034265557975
1116	9	0.636772486772486
1116	10	-1.5560027714789584
1116	11	1.4188334593096457
1116	12	-0.08138699924414539
1116	13	2.401196775006291
1116	14	0.6443940539178712
1116	15	0.348481985386762
1116	16	2.0976883345931014
1116	17	0.764619551524305
1116	18	2.097524565381711
1116	19	-0.13206727135299445
1116	20	2.0715671453766618
1116	21	2.346680524061483
1116	22	1.8494142101285007
1116	23	2.7461766187956727
1116	24	2.7187956664147137
1116	25	4.047335600907033
1201	1	-0.3563177122700836
1201	2	-1.1661753590325077
1201	3	1.5590702947845791
1201	4	-1.5456979087931515
1201	5	1.4761211892164283
1201	6	0.5684177374653601
1201	7	-0.8277588813303112
1201	8	1.5075711766188036
1201	9	0.06534391534391659
1201	10	1.1900289745527814
1201	11	-1.3748173343411452
1201	12	0.7440098261526842
1201	13	-0.5511841773746582
1201	14	0.6602670697908835
1201	15	-3.4292957923910166
1201	16	-0.7594545225497527
1201	17	-1.235380448475695
1201	18	0.7324452003023438
1201	19	-0.9415910808768047
1201	20	-2.706210632401117
1201	21	-0.5898274124464535
1201	22	0.3256046863189823
1201	23	-2.047474174855118
1201	24	-3.0431090954900526
1201	25	-2.1113945578231252
1202	1	0.08812673217435929
1202	2	-0.5312547241118715
1202	3	-0.9647392290249464
1202	4	0.3114449483497026
1202	5	-2.095307382212141
1202	6	0.18746535651298046
1202	7	-1.621409674981102
1202	8	1.0155076845553097
1202	9	0.19232804232804313
1202	10	0.9995527840765916
1202	11	-1.1367220962459115
1202	12	0.029724111866968883
1202	13	-0.15435878054925922
1202	14	-0.8000503905265717
1202	15	0.6818153187200977
1202	16	0.2087994457042157
1202	17	-0.7909360040312521
1202	18	0.6530801209372683
1202	19	-1.0209561602418802
1202	20	0.2937893675988832
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2212	11	-1.3033887629125758
2212	12	-2.595275888133031
2212	13	-1.5154698916603735
2212	14	-4.536161501637686
2212	15	-2.9292957923910166
2212	16	-5.1523116654068986
2212	17	-3.610380448475695
2212	18	-5.99969765684051
2212	19	-2.0487339380196588
2212	20	-7.706210632401117
2212	21	-4.500541698160738
2212	22	-4.692252456538164
2212	23	-5.351045603426549
2212	24	-2.364537666918622
2212	25	-5.02210884353741
2213	1	0.14368228772991642
2213	2	-1.43403250188965
2213	3	-0.5480725623582785
2213	4	0.3114449483497026
2213	5	-0.5953073822121411
2213	6	-0.27086797682035524
2213	7	0.9758125472411194
2213	8	1.5432854623330883
2213	9	-0.863227513227514
2213	10	-1.7921138825900726
2213	11	-2.4283887629125758
2213	12	-0.09527588813303112
2213	13	-0.7654698916603735
2213	14	-1.536161501637686
2213	15	-2.0542957923910166
2213	16	-0.40231166540689856
2213	17	1.889619551524305
2213	18	-4.12469765684051
2213	19	-2.6737339380196588
2213	20	-2.956210632401117
2213	21	-1.7505416981607382
2213	22	-1.4422524565381636
2213	23	1.2739543965734512
2213	24	0.385462333081378
2213	25	-1.89710884353741
2214	1	-0.022984378936751426
2214	2	-0.19792139077853932
2214	3	-1.2980725623582785
2214	4	0.9781116150163669
2214	5	-0.5397518266565839
2214	6	-0.2569790879314695
2214	7	0.0452569916855623
2214	8	-1.0956034265557975
2214	9	0.7478835978836003
2214	10	1.4439972285210416
2214	11	-0.025610985134797204
2214	12	-1.8591647770219168
2214	13	-0.15435878054925922
2214	14	0.7555051650289784
2214	15	1.2373708742756477
2214	16	-2.4578672209624557
2214	17	-0.12426933736458068
2214	18	-0.5691421012849531
2214	19	0.7568216175358984
2214	20	-0.03954396573445251
2214	21	1.5689027462837046
2214	22	-0.9283635676492779
2214	23	1.5239543965734512
2214	24	1.4965734441924923
2214	25	-2.39710884353741
2215	1	1.1436822877299164
2215	2	1.8576341647770143
2215	3	2.535260770975057
2215	4	2.3114449483497026
2215	5	2.2380259511211946
2215	6	2.1874653565129805
2215	7	1.2674792139077837
2215	8	2.126618795666424
2215	9	3.636772486772486
2215	10	1.166219450743263
2215	11	2.02994457042076
2215	12	2.529724111866969
2215	13	0.4011967750062908
2215	14	2.4221718316956498
2215	15	3.5707042076089834
2215	16	2.7643550012597657
2215	17	2.264619551524305
2215	18	2.041969009826154
2215	19	2.534599395313677
2215	20	2.627122700932219
2215	21	1.791124968505926
2215	22	0.6827475434618364
2215	23	3.1906210632401155
2215	24	3.5521289997480423
2215	25	1.7695578231292544
2216	1	-3.5229843789367514
2216	2	-3.475699168556318
2216	3	-1.4647392290249464
2216	4	-1.8552217183169653
2216	5	-1.7619740488788054
2216	6	0.5207986898463091
2216	7	-0.8991874527588806
2216	8	-0.5400478710002474
2216	9	0.636772486772486
2216	10	-2.1671138825900726
2216	11	-2.1367220962459115
2216	12	-2.970275888133031
2216	13	-3.4321365583270413
2216	14	-6.411161501637686
2216	15	-2.2626291257243523
2216	16	-5.9023116654068986
2216	17	-0.5687137818090306
2216	18	0.20863567649282544
2216	19	-1.2987339380196588
2216	20	-3.706210632401117
2216	21	-0.37554169816073824
2216	22	-0.1505857898714993
2216	23	-1.8093789367598845
2216	24	-5.447871000251954
2216	25	-4.730442176870742
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8.0
3	0.0
4	1.0
5	0.0
6	2.0
7	1.0
8	0.0
9	1.0
10	5.0
11	7.0
12	10.0
13	16.0
14	14.0
15	26.0
16	39.0
17	50.0
18	50.0
19	24.0
20	34.0
21	34.0
22	44.0
23	37.0
24	35.0
25	37.0
26	43.0
27	40.0
28	52.0
29	67.0
30	80.0
31	97.0
32	138.0
33	202.0
34	259.0
35	415.0
36	565.0
37	1004.0
38	1742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.26570048309179	17.043478260869566	11.400966183574878	20.28985507246377
2	9.59566647320565	25.3240472044883	38.40201199458309	26.67827432772296
3	21.684414327202322	30.977734753146173	33.39787028073572	13.939980638915781
4	12.584704743465633	20.832526621490803	31.442400774443367	35.14036786060019
5	7.881487219209915	48.547637490317584	32.90085205267235	10.670023237800155
6	29.727114379717438	27.133733307528544	27.694987420166438	15.444164892587576
7	20.546088303640587	40.06584043377227	19.732765298218435	19.655305964368708
8	19.244917715392063	37.36689254598258	28.325266214908034	15.062923523717329
9	27.97676669893514	24.607938044530496	15.58567279767667	31.8296224588577
10	13.206816421378775	26.529821843532147	29.10534469403563	31.158017041053448
11	25.561145510835914	29.489164086687307	20.762383900928793	24.187306501547987
12	28.184281842818425	27.371273712737125	22.00929152148664	22.4351529229578
13	27.960526315789476	19.96904024767802	34.40402476780186	17.66640866873065
14	33.01722469518096	24.385523514611958	21.424424230694793	21.17282755951229
15	35.08805883491387	19.605186762144378	23.727501451519256	21.57925295142249
16	25.130687318489837	22.323330106485965	33.39787028073572	19.14811229428848
17	19.744483159117305	34.12698412698413	28.745644599303137	17.38288811459543
18	20.267131242740998	21.583430120015485	41.94734804490902	16.202090592334496
19	22.95335784788078	36.17185988000774	20.55351267660151	20.321269595509968
20	18.424617766595702	33.55912521772789	28.798142055351267	19.218114960325142
21	19.473480449090204	35.21099496709253	24.00309717382888	21.312427409988384
22	18.15366750532224	32.99787110508999	17.785949293593962	31.062512095993807
23	23.331398723157285	24.840394660475916	20.622944476687945	31.20526213967885
24	17.26340236113799	32.920456744726145	17.340816721501838	32.475324172634025
25	22.80905397562391	33.64287096150126	20.19733023795705	23.35074482491778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	32.0
2	28.5
3	25.0
4	25.0
5	25.0
6	35.5
7	46.0
8	46.0
9	46.0
10	37.0
11	28.0
12	28.0
13	28.0
14	28.0
15	28.0
16	28.0
17	28.0
18	29.0
19	30.0
20	30.0
21	30.0
22	50.0
23	70.0
24	70.0
25	70.0
26	77.0
27	84.0
28	84.0
29	84.0
30	102.0
31	120.0
32	120.0
33	120.0
34	132.5
35	145.0
36	145.0
37	145.0
38	322.0
39	499.0
40	499.0
41	499.0
42	1290.0
43	2081.0
44	2081.0
45	2081.0
46	1481.5
47	882.0
48	882.0
49	882.0
50	652.5
51	423.0
52	423.0
53	423.0
54	333.5
55	244.0
56	244.0
57	244.0
58	197.0
59	150.0
60	150.0
61	150.0
62	139.0
63	128.0
64	128.0
65	128.0
66	103.0
67	78.0
68	78.0
69	78.0
70	61.0
71	44.0
72	44.0
73	44.0
74	31.5
75	19.0
76	19.0
77	19.0
78	16.0
79	13.0
80	13.0
81	13.0
82	10.0
83	7.0
84	7.0
85	7.0
86	3.5
87	0.0
88	0.0
89	0.0
90	1.0
91	2.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07723498744931453
2	0.19308746862328635
3	0.27032245607260086
4	0.27032245607260086
5	0.28963120293492955
6	0.23170496234794363
7	0.28963120293492955
8	0.27032245607260086
9	0.27032245607260086
10	0.28963120293492955
11	0.21239621548561496
12	0.2510137092102723
13	0.21239621548561496
14	0.23170496234794363
15	0.23170496234794363
16	0.27032245607260086
17	0.2510137092102723
18	0.2510137092102723
19	0.23170496234794363
20	0.23170496234794363
21	0.2510137092102723
22	0.23170496234794363
23	0.19308746862328635
24	0.23170496234794363
25	0.19308746862328635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.47209886078393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.72823779193206	39.89187101757096
2	5.562632696390659	5.058891677930102
3	1.7409766454352442	2.374975864066422
4	0.8067940552016986	1.4674647615369762
5	0.5520169851380042	1.2550685460513613
6	0.3821656050955414	1.0426723305657464
7	0.3397027600849257	1.0812898242904034
8	0.12738853503184713	0.46340992469588727
9	0.25477707006369427	1.0426723305657464
>10	1.9957537154989384	17.493724657269745
>50	0.3397027600849257	10.716354508592392
>100	0.16985138004246286	18.11160455686426
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	391	7.549720023170496	No Hit
GCTTATGTACTCTGCGTTGATACCA	270	5.213361652828731	No Hit
CTTATGTACTCTGCGTTGATACCAC	176	3.3983394477698394	No Hit
TATGTACTCTGCGTTGATACCACTG	101	1.9501834330951922	No Hit
GAGTACATAAGCAGTGGTATCAACG	96	1.853639698783549	No Hit
GCGTTGATACCACTGCTTATGTACT	88	1.6991697238849197	No Hit
CATAAGCAGTGGTATCAACGCAGAG	70	1.3516122803630044	No Hit
GTATCAACGCAGAGTACATAAGCAG	67	1.2936860397760186	No Hit
CCCATGTACTCTGCGTTGATACCAC	67	1.2936860397760186	No Hit
ACTCTGCGTTGATACCACTGCTTAT	60	1.1585248117397182	No Hit
ATCATGTACTCTGCGTTGATACCAC	55	1.061981077428075	No Hit
CATGTACTCTGCGTTGATACCACTG	52	1.004054836841089	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	49	0.9461285962541031	No Hit
GTACATGATAAGCAGTGGTATCAAC	47	0.9075111025294458	No Hit
TATCAACGCAGAGTACATAAGCAGT	45	0.8688936088047886	No Hit
CTGCTTATGTACTCTGCGTTGATAC	44	0.8495848619424599	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	34	0.6564973933191736	No Hit
GTGGTATCAACGCAGAGTACATAAG	32	0.6178798995945163	No Hit
GGTATCAACGCAGAGTACATAAGCA	31	0.5985711527321876	No Hit
GTACTCTGCGTTGATACCACTGCTT	30	0.5792624058698591	No Hit
GCAGTGGTATCAACGCAGAGTACAT	30	0.5792624058698591	No Hit
GTACATGGGAAGCAGTGGTATCAAC	27	0.5213361652828732	No Hit
GAGTACATGATAAGCAGTGGTATCA	25	0.48271867155821585	No Hit
TATCAACGCAGAGTACATGATAAGC	21	0.40548368410890134	No Hit
CCACTGCTTATGTACTCTGCGTTGA	21	0.40548368410890134	No Hit
GTGGTATCAACGCAGAGTACATGGG	20	0.3861749372465727	No Hit
GCTTATCATGTACTCTGCGTTGATA	20	0.3861749372465727	No Hit
ATACCACTGCTTATGTACTCTGCGT	19	0.36686619038424406	No Hit
GTTGATACCACTGCTTATGTACTCT	19	0.36686619038424406	No Hit
GCTTATCCCATGTACTCTGCGTTGA	19	0.36686619038424406	No Hit
ACATAAGCAGTGGTATCAACGCAGA	18	0.3475574435219154	No Hit
GATACCACTGCTTATGTACTCTGCG	18	0.3475574435219154	No Hit
GCGTTGATACCACTGCTTATCATGT	17	0.3282486966595868	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	17	0.3282486966595868	No Hit
CAGTGGTATCAACGCAGAGTACATA	15	0.28963120293492955	No Hit
GCAGAGTACATAAGCAGTGGTATCA	15	0.28963120293492955	No Hit
GGATAAGCAGTGGTATCAACGCAGA	15	0.28963120293492955	No Hit
TGATACCACTGCTTATGTACTCTGC	14	0.27032245607260086	No Hit
GTACATGGGATAAGCAGTGGTATCA	14	0.27032245607260086	No Hit
CCCCATGTACTCTGCGTTGATACCA	13	0.2510137092102723	No Hit
CTGCTTATCATGTACTCTGCGTTGA	13	0.2510137092102723	No Hit
GATAAGCAGTGGTATCAACGCAGAG	13	0.2510137092102723	No Hit
CATGTAAGCAGTGGTATCAACGCAG	13	0.2510137092102723	No Hit
GGTATCAACGCAGAGTACATGATAA	13	0.2510137092102723	No Hit
GCGTTGATACCACTGCTTCCCATGT	13	0.2510137092102723	No Hit
GAGTACATGGGAAGCAGTGGTATCA	13	0.2510137092102723	No Hit
GCGTTGATACCACTGCTTATCCCAT	12	0.23170496234794363	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	12	0.23170496234794363	No Hit
GCTTACATGTACTCTGCGTTGATAC	12	0.23170496234794363	No Hit
CAGTGGTATCAACGCAGAGTACATG	11	0.21239621548561496	No Hit
TACCACTGCTTATCATGTACTCTGC	11	0.21239621548561496	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	11	0.21239621548561496	No Hit
GTACATGGTAAGCAGTGGTATCAAC	10	0.19308746862328635	No Hit
GTACATGTAAGCAGTGGTATCAACG	10	0.19308746862328635	No Hit
ATCAACGCAGAGTACATAAGCAGTG	10	0.19308746862328635	No Hit
AAAAAGTACTCTGCGTTGATACCAC	10	0.19308746862328635	No Hit
ACGCAGAGTACATGATAAGCAGTGG	10	0.19308746862328635	No Hit
GTATCAACGCAGAGTACATGATAAG	10	0.19308746862328635	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10	0.19308746862328635	No Hit
GTTGATACCACTGCTTATCATGTAC	9	0.1737787217609577	No Hit
TACCACTGCTTATGTACTCTGCGTT	9	0.1737787217609577	No Hit
ACATGATAAGCAGTGGTATCAACGC	9	0.1737787217609577	No Hit
CATGATAAGCAGTGGTATCAACGCA	9	0.1737787217609577	No Hit
GTATCAACGCAGAGTACATGGGAAG	9	0.1737787217609577	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9	0.1737787217609577	No Hit
ACGCAGAGTACATGGGATAAGCAGT	8	0.15446997489862907	No Hit
GTACTGGTTCACTATCGGTCAGTCA	8	0.15446997489862907	No Hit
GGTATCAACGCAGAGTACATGGGAT	8	0.15446997489862907	No Hit
GAGTACATGTAAGCAGTGGTATCAA	7	0.13516122803630043	No Hit
ACCATGTACTCTGCGTTGATACCAC	7	0.13516122803630043	No Hit
GATACCACTGCTTATCATGTACTCT	7	0.13516122803630043	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	7	0.13516122803630043	No Hit
GAGTACATGGGATAAGCAGTGGTAT	7	0.13516122803630043	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	7	0.13516122803630043	No Hit
CTGCGTTGATACCACTGCTTATGTA	7	0.13516122803630043	No Hit
CCATGTACTCTGCGTTGATACCACT	7	0.13516122803630043	No Hit
ATGTACTCTGCGTTGATACCACTGC	6	0.11585248117397182	No Hit
TGATAAGCAGTGGTATCAACGCAGA	6	0.11585248117397182	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6	0.11585248117397182	No Hit
ATGATAAGCAGTGGTATCAACGCAG	6	0.11585248117397182	No Hit
TATCAACGCAGAGTACATGGGATAA	6	0.11585248117397182	No Hit
GGATACCACGTGTCCCGCCCTACTC	6	0.11585248117397182	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6	0.11585248117397182	No Hit
AAAGTACTCTGCGTTGATACCACTG	6	0.11585248117397182	No Hit
GCTTACCATGTACTCTGCGTTGATA	6	0.11585248117397182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTATG	75	5.405236E-7	13.933332	1
GTACATA	85	2.2268996E-6	12.294118	1
TACATAA	85	2.2268996E-6	12.294118	2
ACATAAG	85	2.2268996E-6	12.294118	3
TGCGTTG	115	4.0610757E-7	10.73913	13
CTGCGTT	115	4.0610757E-7	10.73913	12
CTTATGT	100	1.3757508E-5	10.45	2
CATAAGC	100	1.3757508E-5	10.45	4
TTATGTA	100	1.3757508E-5	10.45	3
CTCTGCG	120	7.0608075E-7	10.291667	10
GTACTCT	120	7.0608075E-7	10.291667	7
TCTGCGT	125	1.1981138E-6	9.88	11
GCGTTGA	125	1.1981138E-6	9.88	14
CGTTGAT	125	1.1981138E-6	9.88	15
TACTCTG	125	1.1981138E-6	9.88	8
GTTGATA	130	1.9879317E-6	9.5	16
ATAAGCA	110	3.961068E-5	9.5	5
TAAGCAG	110	3.961068E-5	9.5	6
TATGTAC	110	3.961068E-5	9.5	4
ACTCTGC	130	1.9879317E-6	9.5	9
>>END_MODULE