FastQCFastQC Report
Wed 25 May 2016
SRR1295221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA4178.051747441591042No Hit
GCTTATGTACTCTGCGTTGATACCA2625.058891677930102No Hit
CTTATGTACTCTGCGTTGATACCAC1643.1666344854218957No Hit
GAGTACATAAGCAGTGGTATCAACG1062.0467271674068352No Hit
GCGTTGATACCACTGCTTATGTACT871.6798609770225914No Hit
CATAAGCAGTGGTATCAACGCAGAG861.6605522301602627No Hit
GTACATGATAAGCAGTGGTATCAAC761.4674647615369762No Hit
TATGTACTCTGCGTTGATACCACTG731.4095385209499904No Hit
CCCATGTACTCTGCGTTGATACCAC721.3902297740876617No Hit
CATGTACTCTGCGTTGATACCACTG691.3323035335006759No Hit
CTGCTTATGTACTCTGCGTTGATAC691.3323035335006759No Hit
ACGCAGAGTACATAAGCAGTGGTAT631.216451052326704No Hit
GTATCAACGCAGAGTACATAAGCAG611.1778335586020467No Hit
ACTCTGCGTTGATACCACTGCTTAT531.0233635837034176No Hit
GGTATCAACGCAGAGTACATAAGCA521.004054836841089No Hit
ATCATGTACTCTGCGTTGATACCAC460.8882023556671172No Hit
TATCAACGCAGAGTACATAAGCAGT440.8495848619424599No Hit
GTGGTATCAACGCAGAGTACATAAG310.5985711527321876No Hit
AAGCAGTGGTATCAACGCAGAGTAC290.5599536590075305No Hit
GTACATGGGATAAGCAGTGGTATCA290.5599536590075305No Hit
ATACCACTGCTTATGTACTCTGCGT280.5406449121452017No Hit
GTACTCTGCGTTGATACCACTGCTT270.5213361652828732No Hit
GTATCAACGCAGAGTACTTTTTTTT270.5213361652828732No Hit
ACATAAGCAGTGGTATCAACGCAGA260.5020274184205445No Hit
GCAGTGGTATCAACGCAGAGTACAT250.48271867155821585No Hit
GTACTTTTTTTTTTTTTTTTTTTTT250.48271867155821585No Hit
GTACATGGGAAGCAGTGGTATCAAC230.4441011778335586No Hit
ACGCAGAGTACATGATAAGCAGTGG220.42479243097122993No Hit
TATCAACGCAGAGTACTTTTTTTTT210.40548368410890134No Hit
GTTGATACCACTGCTTATGTACTCT200.3861749372465727No Hit
GATACCACTGCTTATGTACTCTGCG200.3861749372465727No Hit
GAGTACATGATAAGCAGTGGTATCA190.36686619038424406No Hit
GCTTATCATGTACTCTGCGTTGATA190.36686619038424406No Hit
GCTTATCCCATGTACTCTGCGTTGA190.36686619038424406No Hit
GGTATCAACGCAGAGTACTTTTTTT190.36686619038424406No Hit
TGATACCACTGCTTATGTACTCTGC180.3475574435219154No Hit
CAGTGGTATCAACGCAGAGTACATA180.3475574435219154No Hit
ATCAACGCAGAGTACATAAGCAGTG170.3282486966595868No Hit
GCAGAGTACATAAGCAGTGGTATCA160.30893994979725814No Hit
GTACACAAGCAGTGGTATCAACGCA160.30893994979725814No Hit
TATCAACGCAGAGTACATGATAAGC150.28963120293492955No Hit
GCGTTGATACCACTGCTTATCATGT150.28963120293492955No Hit
CCACTGCTTATGTACTCTGCGTTGA150.28963120293492955No Hit
CTGCTTATCATGTACTCTGCGTTGA150.28963120293492955No Hit
GAGTACATGTAAGCAGTGGTATCAA140.27032245607260086No Hit
CCCCATGTACTCTGCGTTGATACCA140.27032245607260086No Hit
GATAAGCAGTGGTATCAACGCAGAG140.27032245607260086No Hit
TACCACTGCTTATCATGTACTCTGC120.23170496234794363No Hit
GTATCAACGCAGAGTACATGGGAAG120.23170496234794363No Hit
TACATGTACTCTGCGTTGATACCAC110.21239621548561496No Hit
TACCACTGCTTATGTACTCTGCGTT110.21239621548561496No Hit
CATGTAAGCAGTGGTATCAACGCAG110.21239621548561496No Hit
GGATAAGCAGTGGTATCAACGCAGA110.21239621548561496No Hit
GAGTACATGGGAAGCAGTGGTATCA110.21239621548561496No Hit
GTATCAACGCAGAGTACATGATAAG110.21239621548561496No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110.21239621548561496No Hit
ACCATGTACTCTGCGTTGATACCAC100.19308746862328635No Hit
GTACATGGTAAGCAGTGGTATCAAC100.19308746862328635No Hit
GAGTACATGGGATAAGCAGTGGTAT100.19308746862328635No Hit
ATGATAAGCAGTGGTATCAACGCAG100.19308746862328635No Hit
CTTATACACATCTCCGAGCCCACGA100.19308746862328635No Hit
TCCATGTACTCTGCGTTGATACCAC90.1737787217609577No Hit
GGGATAAGCAGTGGTATCAACGCAG90.1737787217609577No Hit
TACATAAGCAGTGGTATCAACGCAG90.1737787217609577No Hit
CATGATAAGCAGTGGTATCAACGCA90.1737787217609577No Hit
AACGCAGAGTACATAAGCAGTGGTA90.1737787217609577No Hit
GAGTACACAAGCAGTGGTATCAACG90.1737787217609577No Hit
GTGGTATCAACGCAGAGTACATGGG80.15446997489862907No Hit
CAGTGGTATCAACGCAGAGTACATG80.15446997489862907No Hit
GTACATGTAAGCAGTGGTATCAACG80.15446997489862907No Hit
GTGGTATCAACGCAGAGTACTTTTT80.15446997489862907No Hit
CTCTGCGTTGATACCACTGCTTATG80.15446997489862907No Hit
GGTATCAACGCAGAGTACATGATAA80.15446997489862907No Hit
GTATCAACGCAGAGTACATGGGATA80.15446997489862907No Hit
ACTCTGCGTTGATACCACTGCTTCC70.13516122803630043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70.13516122803630043No Hit
ACGCAGAGTACATGGGAAGCAGTGG70.13516122803630043No Hit
GAGTACTTTTTTTTTTTTTTTTTTT70.13516122803630043No Hit
AAAAAGTACTCTGCGTTGATACCAC70.13516122803630043No Hit
GCGTTGATACCACTGCTTCCCATGT70.13516122803630043No Hit
CTGCGTTGATACCACTGCTTATGTA70.13516122803630043No Hit
ATGTACTCTGCGTTGATACCACTGC60.11585248117397182No Hit
GTCAGGAGTATTTAGCCTTGGAGGA60.11585248117397182No Hit
GATACCACTGCTTATCATGTACTCT60.11585248117397182No Hit
GTTGATACCACTGCTTCCCATGTAC60.11585248117397182No Hit
GGTAAGCAGTGGTATCAACGCAGAG60.11585248117397182No Hit
GCGTTGATACCACTGCTTATCCCAT60.11585248117397182No Hit
ATACAGGGTGACAGCCCCGTACACA60.11585248117397182No Hit
ACGCAGAGTACATGGGATAAGCAGT60.11585248117397182No Hit
ACATGATAAGCAGTGGTATCAACGC60.11585248117397182No Hit
GTACATGGGCAGTGGTATCAACGCA60.11585248117397182No Hit
GTACTGGTTCACTATCGGTCAGTCA60.11585248117397182No Hit
GCTTGTGTACTCTGCGTTGATACCA60.11585248117397182No Hit
GCTTACATGTACTCTGCGTTGATAC60.11585248117397182No Hit
CCATGTACTCTGCGTTGATACCACT60.11585248117397182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA951.0397648E-410.01
TACATAA951.0397648E-410.02
ACATAAG951.0397648E-410.03
TCAACGC1001.7506762E-49.518
CAACGCA1001.7506762E-49.519
TGGTATC1052.8675838E-49.0476213
AGCAGTG1201.0323371E-48.7083348
AAGCAGT1201.0323371E-48.7083347
GGTATCA1104.5812025E-48.63636414
CATAAGC1104.5812025E-48.6363644
GCAGTGG1251.6137456E-48.369
TAAGCAG1157.154177E-48.2608696
GTATCAA1157.154177E-48.26086915
ATAAGCA1200.00109420437.9166675
AGTGGTA1200.00109420437.91666711
TATCAAC1200.00109420437.91666716
GTGGTAT1200.00109420437.91666712
ATCAACG1200.00109420437.91666717
CAGTGGT1200.00109420437.91666710
Produced by FastQC (version 0.11.5)