##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295221_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5179 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.256999420737593 31.0 31.0 33.0 28.0 34.0 2 31.053871403745898 31.0 31.0 33.0 28.0 34.0 3 31.369955589882217 31.0 31.0 34.0 28.0 34.0 4 35.07974512454142 37.0 35.0 37.0 32.0 37.0 5 35.254682371114114 37.0 35.0 37.0 32.0 37.0 6 35.23556671172041 37.0 35.0 37.0 32.0 37.0 7 35.16122803630044 37.0 35.0 37.0 32.0 37.0 8 35.125506854605135 37.0 35.0 37.0 32.0 37.0 9 36.78335586020467 39.0 37.0 39.0 32.0 39.0 10 36.75477891484843 39.0 37.0 39.0 32.0 39.0 11 36.85981849777949 39.0 37.0 39.0 33.0 39.0 12 36.810001930874684 39.0 37.0 39.0 32.0 39.0 13 36.441977215678705 39.0 37.0 39.0 32.0 39.0 14 37.69376327476347 40.0 37.0 41.0 32.0 41.0 15 37.527128789341575 40.0 37.0 41.0 32.0 41.0 16 37.145394863873335 39.0 37.0 41.0 31.0 41.0 17 37.55049237304499 40.0 37.0 41.0 32.0 41.0 18 37.59509557829697 40.0 37.0 41.0 32.0 41.0 19 37.79320332110446 40.0 38.0 41.0 32.0 41.0 20 37.895153504537554 40.0 38.0 41.0 32.0 41.0 21 37.87333462058312 40.0 38.0 41.0 32.0 41.0 22 37.914076076462635 40.0 38.0 41.0 32.0 41.0 23 37.74570380382313 40.0 38.0 41.0 32.0 41.0 24 37.764626375748215 40.0 38.0 41.0 32.0 41.0 25 36.533307588337514 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37282060972538034 1101 2 -0.7526769967246238 1101 3 0.5626039304610693 1101 4 0.5952506928697403 1101 5 -0.12690224237842784 1101 6 0.0011778785588347773 1101 7 -0.0202191987906275 1101 8 0.5772675736961475 1101 9 1.0153817082388485 1101 10 0.6369614512471671 1101 11 -0.6664588057445116 1101 12 0.6572499370118408 1101 13 1.7832073570168774 1101 14 1.1587175610985057 1101 15 0.7912635424540113 1101 16 1.9340765936004018 1101 17 1.630713025951124 1101 18 0.28134290753338576 1101 19 1.696888384983609 1101 20 1.8147770219198733 1101 21 0.20385487528344726 1101 22 -0.7840136054421762 1101 23 0.4389518770471099 1101 24 0.5356449987402314 1101 25 1.529944570420767 1102 1 -0.4327349458301768 1102 2 -0.086010330057956 1102 3 -0.18739606953893073 1102 4 -1.821415973796924 1102 5 0.011986646510457888 1102 6 0.5289556563366133 1102 7 1.1047808012093725 1102 8 0.9106009070294832 1102 9 -0.51239606953893 1102 10 0.9425170068027242 1102 11 0.5002078609221527 1102 12 0.9628054925673979 1102 13 0.9776518014613202 1102 14 -2.3412824389014943 1102 15 0.8468190980095684 1102 16 -0.9409234063995982 1102 17 0.28349080372890256 1102 18 -0.9825459813555 1102 19 -0.46977828168305535 1102 20 0.3842214663643162 1102 21 0.342743764172333 1102 22 -1.6451247165532905 1102 23 -0.505492567397333 1102 24 -1.4226883345931043 1102 25 -0.595055429579233 1103 1 0.15059838750315535 1103 2 0.24732300327537615 1103 3 0.1181594860166264 1103 4 -1.071415973796924 1103 5 -0.2380133534895421 1103 6 -1.443266565885608 1103 7 0.8686696900982582 1103 8 -0.42273242630385255 1103 9 0.4598261526832914 1103 10 0.5258503401360599 1103 11 -0.5553476946334044 1103 12 0.6572499370118408 1103 13 0.4498740236835417 1103 14 -2.17461577223483 1103 15 -1.0976253464348744 1103 16 -0.9548122952884839 1103 17 0.5196019148400168 1103 18 0.9480095742000572 1103 19 -0.08088939279416962 1103 20 -1.851889644746798 1103 21 0.9816326530612187 1103 22 -1.3395691609977334 1103 23 0.994507432602667 1103 24 2.2023116654068957 1103 25 -1.1367220962458973 1104 1 -0.9327349458301768 1104 2 -0.586010330057956 1104 3 -0.18739606953893073 1104 4 -0.196415973796924 1104 5 -0.9880133534895421 1104 6 -0.9710443436633867 1104 7 -0.6452191987906275 1104 8 -1.5893990929705168 1104 9 -0.26239606953893 1104 10 -1.0574829931972758 1104 11 -1.7497921390778473 1104 12 -2.412194507432602 1104 13 -3.0223481985386798 1104 14 -4.091282438901494 1104 15 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1106 3 0.9911753590324999 1106 4 0.928584026203076 1106 5 -0.09515621063239621 1106 6 1.207527084908044 1106 7 0.9797808012093725 1106 8 0.053458049886629055 1106 9 0.3804610733182159 1106 10 0.9068027210884395 1106 11 0.5716364323507221 1106 12 0.9270912068531132 1106 13 1.6562232300327508 1106 14 1.0158604182413598 1106 15 -2.6531809019904316 1106 16 1.2197908793146865 1106 17 1.5513479465860485 1106 18 1.3924540186445 1106 19 0.74450743260266 1106 20 1.1163643235071703 1106 21 0.5213151927437636 1106 22 0.5334467120181401 1106 23 1.4230788611740977 1106 24 1.916597379692611 1106 25 -1.0414840010078024 1107 1 0.039487276392044635 1107 2 -0.1971214411690667 1107 3 -1.8818405139833736 1107 4 -1.071415973796924 1107 5 0.4286533131771222 1107 6 0.44562232300327764 1107 7 0.9797808012093725 1107 8 -0.7560657596371811 1107 9 -2.206840513983373 1107 10 -2.807482993197276 1107 11 -0.22201436130006869 1107 12 -0.34275006298815924 1107 13 -0.5501259763164583 1107 14 0.047606449987391386 1107 15 -3.0976253464348744 1107 16 -1.9548122952884839 1107 17 -0.5915091962710974 1107 18 -0.38532375913327854 1107 19 0.36355505165028035 1107 20 1.4814436885865376 1107 21 0.7594104308389973 1107 22 -1.0062358276644048 1107 23 -0.7832703451751115 1107 24 -2.4643550012597686 1107 25 -1.8033887629125687 1108 1 -0.7827349458301782 1108 2 -1.3860103300579567 1108 3 -1.4373960695389307 1108 4 0.22858402620307317 1108 5 0.061986646510455046 1108 6 -0.12104434366338523 1108 7 -0.7202191987906303 1108 8 -0.08939909297051685 1108 9 0.4376039304610728 1108 10 -2.0074829931972786 1108 11 -1.4997921390778473 1108 12 -0.6871945074326007 1108 13 -0.7723481985386798 1108 14 -0.34128243890149434 1108 15 -1.8531809019904344 1108 16 -4.265923406399601 1108 17 -3.0915091962710974 1108 18 -2.6075459813555 1108 19 -3.0697782816830568 1108 20 -1.7407785336356838 1108 21 -1.5072562358276684 1108 22 -1.5951247165532934 1108 23 -2.005492567397333 1108 24 -2.997688334593107 1108 25 -2.5700554295792344 1109 1 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1.4776518014613202 1110 14 1.7837175610985057 1110 15 1.4718190980095684 1110 16 0.8090765936004018 1110 17 1.9084908037289026 1110 18 2.5174540186445 1110 19 2.1552217183169446 1110 20 1.8842214663643162 1110 21 -0.03225623582766701 1110 22 0.10487528344670949 1110 23 1.369507432602667 1110 24 -0.04768833459310429 1110 25 0.029944570420767036 1111 1 0.5672650541698232 1111 2 0.788989669942044 1111 3 0.43760393046106927 1111 4 1.178584026203076 1111 5 0.7619866465104579 1111 6 1.0289556563366133 1111 7 0.4797808012093725 1111 8 1.2856009070294832 1111 9 0.73760393046107 1111 10 0.4425170068027242 1111 11 0.8752078609221527 1111 12 1.087805492567398 1111 13 1.3526518014613202 1111 14 1.7837175610985057 1111 15 1.7218190980095684 1111 16 1.0590765936004018 1111 17 2.1584908037289026 1111 18 1.0174540186445 1111 19 1.5302217183169446 1111 20 1.1342214663643162 1111 21 1.717743764172333 1111 22 2.1048752834467095 1111 23 2.244507432602667 1111 24 1.7023116654068957 1111 25 1.529944570420767 1112 1 0.6922650541698232 1112 2 1.413989669942044 1112 3 0.6876039304610693 1112 4 -0.07141597379692399 1112 5 1.0119866465104579 1112 6 1.1539556563366133 1112 7 1.2297808012093725 1112 8 -0.8393990929705168 1112 9 0.11260393046106998 1112 10 1.1925170068027242 1112 11 1.5002078609221527 1112 12 0.08780549256739789 1112 13 0.7276518014613202 1112 14 0.28371756109850566 1112 15 0.5968190980095684 1112 16 -0.6909234063995982 1112 17 1.2834908037289026 1112 18 1.2674540186445 1112 19 -0.8447782816830554 1112 20 0.8842214663643162 1112 21 1.092743764172333 1112 22 0.6048752834467095 1112 23 0.744507432602667 1112 24 1.2023116654068957 1112 25 0.029944570420767036 1113 1 -0.5577349458301768 1113 2 -0.086010330057956 1113 3 -1.0623960695389307 1113 4 -1.696415973796924 1113 5 -1.3630133534895421 1113 6 -0.34604434366338666 1113 7 -2.1452191987906275 1113 8 -0.46439909297051685 1113 9 -3.38739606953893 1113 10 -1.3074829931972758 1113 11 -3.1247921390778473 1113 12 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24 -5.654831191735958 2201 25 -4.041484001007802 2202 1 1.2458364827412538 2202 2 1.4854182413706134 2202 3 0.7054610733182152 2202 4 1.071441169060222 2202 5 1.4762723607961732 2202 6 1.4932413706223286 2202 7 1.6940665154950878 2202 8 1.4820294784580526 2202 9 1.6661753590325006 2202 10 1.6210884353741548 2202 11 0.714493575207868 2202 12 0.7842340639959673 2202 13 0.6562232300327508 2202 14 1.873003275384221 2202 15 2.4896762408667144 2202 16 2.648362307886117 2202 17 2.6942050894431873 2202 18 1.6781683043587847 2202 19 1.8873645754597987 2202 20 2.402078609221462 2202 21 1.5213151927437636 2202 22 0.6763038548752789 2202 23 0.8516502897455211 2202 24 0.7737402368354651 2202 25 1.9585159989921976 2203 1 -3.0827349458301754 2203 2 -2.1860103300579574 2203 3 -2.537396069538932 2203 4 -4.371415973796925 2203 5 -5.8380133534895435 2203 6 -6.421044343663386 2203 7 -3.220219198790627 2203 8 -4.489399092970515 2203 9 -3.562396069538927 2203 10 -3.807482993197276 2203 11 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-3.51239606953893 2206 10 -1.1824829931972758 2206 11 -2.4997921390778473 2206 12 -4.412194507432602 2206 13 -3.7723481985386798 2206 14 0.15871756109850566 2206 15 -2.2781809019904316 2206 16 -1.6909234063995982 2206 17 -4.341509196271097 2206 18 -5.6075459813555 2206 19 -5.844778281683055 2206 20 -3.740778533635684 2206 21 -1.782256235827667 2206 22 -0.8951247165532905 2206 23 -1.130492567397333 2206 24 -1.4226883345931043 2206 25 -1.095055429579233 2207 1 1.8172650541698232 2207 2 1.788989669942044 2207 3 0.8126039304610693 2207 4 1.678584026203076 2207 5 1.2619866465104579 2207 6 1.0289556563366133 2207 7 1.7297808012093725 2207 8 1.1606009070294832 2207 9 1.73760393046107 2207 10 1.9425170068027242 2207 11 1.0002078609221527 2207 12 1.962805492567398 2207 13 2.1026518014613202 2207 14 2.7837175610985057 2207 15 2.3468190980095684 2207 16 2.684076593600402 2207 17 1.9084908037289026 2207 18 2.7674540186445 2207 19 2.5302217183169446 2207 20 1.5092214663643162 2207 21 2.717743764172333 2207 22 2.3548752834467095 2207 23 2.619507432602667 2207 24 2.7023116654068957 2207 25 2.904944570420767 2208 1 1.1922650541698232 2208 2 1.413989669942044 2208 3 1.3126039304610693 2208 4 1.428584026203076 2208 5 0.6369866465104579 2208 6 0.6539556563366133 2208 7 -1.2702191987906275 2208 8 0.7856009070294832 2208 9 0.73760393046107 2208 10 -1.8074829931972758 2208 11 0.25020786092215275 2208 12 1.337805492567398 2208 13 1.1026518014613202 2208 14 0.9087175610985057 2208 15 1.9718190980095684 2208 16 -1.8159234063995982 2208 17 0.28349080372890256 2208 18 1.0174540186445 2208 19 1.9052217183169446 2208 20 2.259221466364316 2208 21 1.342743764172333 2208 22 1.7298752834467095 2208 23 2.244507432602667 2208 24 3.0773116654068957 2208 25 2.529944570420767 2209 1 -1.3077349458301768 2209 2 -0.086010330057956 2209 3 -2.4373960695389307 2209 4 -1.946415973796924 2209 5 0.5119866465104579 2209 6 -2.3460443436633867 2209 7 -0.2702191987906275 2209 8 -1.9643990929705168 2209 9 -1.26239606953893 2209 10 -1.3074829931972758 2209 11 -1.6247921390778473 2209 12 -2.287194507432602 2209 13 -0.27234819853867975 2209 14 -2.4662824389014943 2209 15 -2.1531809019904316 2209 16 -2.440923406399598 2209 17 -3.0915091962710974 2209 18 -3.3575459813555 2209 19 -0.21977828168305535 2209 20 -4.115778533635684 2209 21 -2.657256235827667 2209 22 -0.2701247165532905 2209 23 -0.005492567397332948 2209 24 -2.5476883345931043 2209 25 -3.220055429579233 2210 1 0.9283761652809375 2210 2 1.4695452254976011 2210 3 1.3403817082388478 2210 4 0.039695137314190276 2210 5 0.8730977576215722 2210 6 0.6678445452254991 2210 7 1.0908919123204868 2210 8 -0.5338435374149597 2210 9 1.0153817082388485 2210 10 1.4147392290249456 2210 11 0.8890967498110385 2210 12 1.1016943814562836 2210 13 1.560985134794656 2210 14 1.4920508944318414 2210 15 0.9023746535651256 2210 16 0.7118543713781804 2210 17 1.8529352481733454 2210 18 1.2813429075333858 2210 19 1.8079994960947232 2210 20 0.48144368858653763 2210 21 0.5371882086167759 2210 22 1.2159863945578238 2210 23 1.5500629881582242 2210 24 1.86897833207356 2210 25 -0.13672209624589726 2211 1 0.0672650541698232 2211 2 0.163989669942044 2211 3 0.5626039304610693 2211 4 0.928584026203076 2211 5 0.3869866465104579 2211 6 1.0289556563366133 2211 7 -0.6452191987906275 2211 8 1.1606009070294832 2211 9 1.73760393046107 2211 10 0.8175170068027242 2211 11 1.3752078609221527 2211 12 1.587805492567398 2211 13 1.4776518014613202 2211 14 0.5337175610985057 2211 15 0.5968190980095684 2211 16 2.434076593600402 2211 17 -0.9665091962710974 2211 18 1.0174540186445 2211 19 0.030221718316944646 2211 20 1.1342214663643162 2211 21 -0.282256235827667 2211 22 0.8548752834467095 2211 23 0.994507432602667 2211 24 1.9523116654068957 2211 25 0.15494457042076704 2212 1 0.8172650541698232 2212 2 -0.086010330057956 2212 3 0.06260393046106927 2212 4 0.428584026203076 2212 5 0.5119866465104579 2212 6 0.40395565633661334 2212 7 1.2297808012093725 2212 8 0.9106009070294832 2212 9 0.48760393046107 2212 10 0.3175170068027242 2212 11 -1.1247921390778473 2212 12 -0.4121945074326021 2212 13 1.1026518014613202 2212 14 0.9087175610985057 2212 15 0.5968190980095684 2212 16 -0.6909234063995982 2212 17 1.2834908037289026 2212 18 -1.6075459813555 2212 19 0.6552217183169446 2212 20 1.8842214663643162 2212 21 0.967743764172333 2212 22 1.2298752834467095 2212 23 -0.005492567397332948 2212 24 -0.04768833459310429 2212 25 0.779944570420767 2213 1 -1.0577349458301768 2213 2 -2.961010330057956 2213 3 -1.5623960695389307 2213 4 -1.321415973796924 2213 5 -1.4880133534895421 2213 6 0.40395565633661334 2213 7 0.3547808012093725 2213 8 0.28560090702948315 2213 9 -1.51239606953893 2213 10 -0.9324829931972758 2213 11 0.8752078609221527 2213 12 -0.7871945074326021 2213 13 0.35265180146132025 2213 14 -0.34128243890149434 2213 15 -0.40318090199043155 2213 16 0.5590765936004018 2213 17 0.40849080372890256 2213 18 -3.1075459813555 2213 19 -3.4697782816830554 2213 20 -1.1157785336356838 2213 21 0.717743764172333 2213 22 -0.1451247165532905 2213 23 0.36950743260266705 2213 24 -0.1726883345931043 2213 25 0.654944570420767 2214 1 0.37282060972538034 2214 2 1.2473230032753797 2214 3 0.22927059712773712 2214 4 1.0396951373141903 2214 5 0.5397644242882365 2214 6 0.7789556563366133 2214 7 0.8686696900982582 2214 8 1.1328231292517046 2214 9 1.5709372637944057 2214 10 1.52585034013606 2214 11 0.22243008314437418 2214 12 -0.23163895187704497 2214 13 -0.43901486520534405 2214 14 0.15871756109850566 2214 15 0.7912635424540113 2214 16 1.6007432602670661 2214 17 0.9640463592844597 2214 18 1.9480095742000572 2214 19 1.252443940539166 2214 20 1.3703325774754305 2214 21 0.5371882086167759 2214 22 1.2159863945578238 2214 23 0.6611740992693314 2214 24 0.6467561098513386 2214 25 -0.0256109851347901 2215 1 0.9839317208364875 2215 2 1.413989669942044 2215 3 1.895937263794405 2215 4 1.5952506928697403 2215 5 1.7619866465104579 2215 6 1.4456223230032776 2215 7 1.3131141345427082 2215 8 0.9106009070294832 2215 9 1.5709372637944057 2215 10 2.192517006802724 2215 11 2.0002078609221527 2215 12 1.8794721592340622 2215 13 2.060985134794656 2215 14 0.6587175610985057 2215 15 2.180152431342904 2215 16 2.934076593600402 2215 17 2.2418241370622383 2215 18 2.2257873519778357 2215 19 0.8635550516502803 2215 20 -0.2407785336356838 2215 21 1.592743764172333 2215 22 1.9382086167800452 2215 23 1.8278407659360028 2215 24 1.5356449987402314 2215 25 3.029944570420767 2216 1 -0.016068279163508947 2216 2 0.5806563366087119 2216 3 -1.7707294028722629 2216 4 1.0952506928697403 2216 5 -0.07134668682287781 2216 6 0.27895565633661334 2216 7 -0.0202191987906275 2216 8 0.24393424036281885 2216 9 -0.76239606953893 2216 10 -0.3074829931972758 2216 11 0.16687452758881705 2216 12 0.7128054925673979 2216 13 -1.439014865205344 2216 14 0.49205089443184136 2216 15 -2.9865142353237673 2216 16 -0.23259007306626245 2216 17 -3.424842529604433 2216 18 -0.6075459813555 2216 19 -2.9697782816830554 2216 20 -0.4074452003023481 2216 21 -1.0739229024943313 2216 22 -0.8951247165532905 2216 23 -2.6721592340639972 2216 24 -6.297688334593104 2216 25 -4.303388762912569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 1.0 14 6.0 15 3.0 16 11.0 17 16.0 18 17.0 19 15.0 20 24.0 21 25.0 22 22.0 23 18.0 24 28.0 25 23.0 26 27.0 27 29.0 28 36.0 29 57.0 30 61.0 31 86.0 32 128.0 33 188.0 34 243.0 35 358.0 36 554.0 37 1015.0 38 2186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.27037466203167 16.164542294322132 11.181923522595596 22.3831595210506 2 10.137092102722534 23.672523653214906 39.19675613052713 26.993628113535433 3 21.08515157366287 31.859432322842245 31.878741069704574 15.176675033790307 4 11.816953079745124 20.505889167793008 30.72021625796486 36.95694149449701 5 7.5883375168951535 48.44564587758254 32.55454720988608 11.411469395636223 6 29.11759026839158 26.60745317628886 29.175516508978568 15.099440046340993 7 21.374782776597797 39.00366866190384 20.312801699169725 19.308746862328636 8 21.181695307974515 36.76385402587372 28.036300444101176 14.018150222050588 9 29.851322649160068 21.258930295423827 16.759992276501254 32.12975477891485 10 12.782390422861557 26.839158138636805 28.538327862521722 31.840123575979916 11 26.337130720216255 28.171461672137475 21.5099440046341 23.981463603012166 12 28.44178412821008 25.970264529832015 22.90017377872176 22.687777563236146 13 27.892601892988218 17.655012555534093 37.06779988410276 17.384585667374928 14 33.54577056778679 22.923908845113942 22.402471996910002 21.127848590189263 15 35.547402973547015 18.710175709596445 24.79243097122997 20.949990345626567 16 24.850357211816952 20.544506661517666 35.16122803630044 19.443908090364936 17 19.888009268198495 33.828924502799765 28.28731415331145 17.995752075690287 18 20.467271674068353 19.79146553388685 43.13574049044217 16.605522301602626 19 22.42178447276941 34.62726921591348 21.591348010814986 21.359598300502125 20 18.497779494110834 32.728325931647035 30.160262598957328 18.613631975284804 21 19.540451824676577 34.13786445259703 24.79243097122997 21.52925275149643 22 17.95713458196563 31.898049816566903 18.941880671944393 31.202934929523074 23 23.90422861556285 23.131878741069706 23.228422475381347 29.7354701679861 24 16.451052326703998 31.878741069704574 18.883954431357406 32.78625217223402 25 23.962154856149837 32.61247345047306 20.853446611314926 22.571925082062176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 9.0 2 13.0 3 17.0 4 17.0 5 17.0 6 27.5 7 38.0 8 38.0 9 38.0 10 29.0 11 20.0 12 20.0 13 20.0 14 18.0 15 16.0 16 16.0 17 16.0 18 24.5 19 33.0 20 33.0 21 33.0 22 41.5 23 50.0 24 50.0 25 50.0 26 65.5 27 81.0 28 81.0 29 81.0 30 106.0 31 131.0 32 131.0 33 131.0 34 137.0 35 143.0 36 143.0 37 143.0 38 309.0 39 475.0 40 475.0 41 475.0 42 1306.5 43 2138.0 44 2138.0 45 2138.0 46 1538.0 47 938.0 48 938.0 49 938.0 50 690.5 51 443.0 52 443.0 53 443.0 54 335.0 55 227.0 56 227.0 57 227.0 58 183.5 59 140.0 60 140.0 61 140.0 62 130.0 63 120.0 64 120.0 65 120.0 66 99.0 67 78.0 68 78.0 69 78.0 70 58.5 71 39.0 72 39.0 73 39.0 74 27.5 75 16.0 76 16.0 77 16.0 78 18.0 79 20.0 80 20.0 81 20.0 82 13.0 83 6.0 84 6.0 85 6.0 86 3.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019308746862328634 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.03861749372465727 14 0.019308746862328634 15 0.0 16 0.0 17 0.0 18 0.0 19 0.019308746862328634 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5179.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.722147132651095 #Duplication Level Percentage of deduplicated Percentage of total 1 85.15884305357989 34.678509364742226 2 6.353722143195828 5.174744159104074 3 2.1337126600284493 2.6066808264143657 4 1.1853959222380275 1.9308746862328634 5 0.6638217164532955 1.3516122803630044 6 0.6638217164532955 1.6219347364356054 7 0.33191085822664773 0.9461285962541031 8 0.33191085822664773 1.0812898242904034 9 0.2844950213371266 1.0426723305657464 >10 2.1811284969179705 16.54759606101564 >50 0.5215742057847321 14.693956362232091 >100 0.1896633475580844 18.324000772349873 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 417 8.051747441591042 No Hit GCTTATGTACTCTGCGTTGATACCA 262 5.058891677930102 No Hit CTTATGTACTCTGCGTTGATACCAC 164 3.1666344854218957 No Hit GAGTACATAAGCAGTGGTATCAACG 106 2.0467271674068352 No Hit GCGTTGATACCACTGCTTATGTACT 87 1.6798609770225914 No Hit CATAAGCAGTGGTATCAACGCAGAG 86 1.6605522301602627 No Hit GTACATGATAAGCAGTGGTATCAAC 76 1.4674647615369762 No Hit TATGTACTCTGCGTTGATACCACTG 73 1.4095385209499904 No Hit CCCATGTACTCTGCGTTGATACCAC 72 1.3902297740876617 No Hit CATGTACTCTGCGTTGATACCACTG 69 1.3323035335006759 No Hit CTGCTTATGTACTCTGCGTTGATAC 69 1.3323035335006759 No Hit ACGCAGAGTACATAAGCAGTGGTAT 63 1.216451052326704 No Hit GTATCAACGCAGAGTACATAAGCAG 61 1.1778335586020467 No Hit ACTCTGCGTTGATACCACTGCTTAT 53 1.0233635837034176 No Hit GGTATCAACGCAGAGTACATAAGCA 52 1.004054836841089 No Hit ATCATGTACTCTGCGTTGATACCAC 46 0.8882023556671172 No Hit TATCAACGCAGAGTACATAAGCAGT 44 0.8495848619424599 No Hit GTGGTATCAACGCAGAGTACATAAG 31 0.5985711527321876 No Hit AAGCAGTGGTATCAACGCAGAGTAC 29 0.5599536590075305 No Hit GTACATGGGATAAGCAGTGGTATCA 29 0.5599536590075305 No Hit ATACCACTGCTTATGTACTCTGCGT 28 0.5406449121452017 No Hit GTACTCTGCGTTGATACCACTGCTT 27 0.5213361652828732 No Hit GTATCAACGCAGAGTACTTTTTTTT 27 0.5213361652828732 No Hit ACATAAGCAGTGGTATCAACGCAGA 26 0.5020274184205445 No Hit GCAGTGGTATCAACGCAGAGTACAT 25 0.48271867155821585 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 25 0.48271867155821585 No Hit GTACATGGGAAGCAGTGGTATCAAC 23 0.4441011778335586 No Hit ACGCAGAGTACATGATAAGCAGTGG 22 0.42479243097122993 No Hit TATCAACGCAGAGTACTTTTTTTTT 21 0.40548368410890134 No Hit GTTGATACCACTGCTTATGTACTCT 20 0.3861749372465727 No Hit GATACCACTGCTTATGTACTCTGCG 20 0.3861749372465727 No Hit GAGTACATGATAAGCAGTGGTATCA 19 0.36686619038424406 No Hit GCTTATCATGTACTCTGCGTTGATA 19 0.36686619038424406 No Hit GCTTATCCCATGTACTCTGCGTTGA 19 0.36686619038424406 No Hit GGTATCAACGCAGAGTACTTTTTTT 19 0.36686619038424406 No Hit TGATACCACTGCTTATGTACTCTGC 18 0.3475574435219154 No Hit CAGTGGTATCAACGCAGAGTACATA 18 0.3475574435219154 No Hit ATCAACGCAGAGTACATAAGCAGTG 17 0.3282486966595868 No Hit GCAGAGTACATAAGCAGTGGTATCA 16 0.30893994979725814 No Hit GTACACAAGCAGTGGTATCAACGCA 16 0.30893994979725814 No Hit TATCAACGCAGAGTACATGATAAGC 15 0.28963120293492955 No Hit GCGTTGATACCACTGCTTATCATGT 15 0.28963120293492955 No Hit CCACTGCTTATGTACTCTGCGTTGA 15 0.28963120293492955 No Hit CTGCTTATCATGTACTCTGCGTTGA 15 0.28963120293492955 No Hit GAGTACATGTAAGCAGTGGTATCAA 14 0.27032245607260086 No Hit CCCCATGTACTCTGCGTTGATACCA 14 0.27032245607260086 No Hit GATAAGCAGTGGTATCAACGCAGAG 14 0.27032245607260086 No Hit TACCACTGCTTATCATGTACTCTGC 12 0.23170496234794363 No Hit GTATCAACGCAGAGTACATGGGAAG 12 0.23170496234794363 No Hit TACATGTACTCTGCGTTGATACCAC 11 0.21239621548561496 No Hit TACCACTGCTTATGTACTCTGCGTT 11 0.21239621548561496 No Hit CATGTAAGCAGTGGTATCAACGCAG 11 0.21239621548561496 No Hit GGATAAGCAGTGGTATCAACGCAGA 11 0.21239621548561496 No Hit GAGTACATGGGAAGCAGTGGTATCA 11 0.21239621548561496 No Hit GTATCAACGCAGAGTACATGATAAG 11 0.21239621548561496 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11 0.21239621548561496 No Hit ACCATGTACTCTGCGTTGATACCAC 10 0.19308746862328635 No Hit GTACATGGTAAGCAGTGGTATCAAC 10 0.19308746862328635 No Hit GAGTACATGGGATAAGCAGTGGTAT 10 0.19308746862328635 No Hit ATGATAAGCAGTGGTATCAACGCAG 10 0.19308746862328635 No Hit CTTATACACATCTCCGAGCCCACGA 10 0.19308746862328635 No Hit TCCATGTACTCTGCGTTGATACCAC 9 0.1737787217609577 No Hit GGGATAAGCAGTGGTATCAACGCAG 9 0.1737787217609577 No Hit TACATAAGCAGTGGTATCAACGCAG 9 0.1737787217609577 No Hit CATGATAAGCAGTGGTATCAACGCA 9 0.1737787217609577 No Hit AACGCAGAGTACATAAGCAGTGGTA 9 0.1737787217609577 No Hit GAGTACACAAGCAGTGGTATCAACG 9 0.1737787217609577 No Hit GTGGTATCAACGCAGAGTACATGGG 8 0.15446997489862907 No Hit CAGTGGTATCAACGCAGAGTACATG 8 0.15446997489862907 No Hit GTACATGTAAGCAGTGGTATCAACG 8 0.15446997489862907 No Hit GTGGTATCAACGCAGAGTACTTTTT 8 0.15446997489862907 No Hit CTCTGCGTTGATACCACTGCTTATG 8 0.15446997489862907 No Hit GGTATCAACGCAGAGTACATGATAA 8 0.15446997489862907 No Hit GTATCAACGCAGAGTACATGGGATA 8 0.15446997489862907 No Hit ACTCTGCGTTGATACCACTGCTTCC 7 0.13516122803630043 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7 0.13516122803630043 No Hit ACGCAGAGTACATGGGAAGCAGTGG 7 0.13516122803630043 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7 0.13516122803630043 No Hit AAAAAGTACTCTGCGTTGATACCAC 7 0.13516122803630043 No Hit GCGTTGATACCACTGCTTCCCATGT 7 0.13516122803630043 No Hit CTGCGTTGATACCACTGCTTATGTA 7 0.13516122803630043 No Hit ATGTACTCTGCGTTGATACCACTGC 6 0.11585248117397182 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 6 0.11585248117397182 No Hit GATACCACTGCTTATCATGTACTCT 6 0.11585248117397182 No Hit GTTGATACCACTGCTTCCCATGTAC 6 0.11585248117397182 No Hit GGTAAGCAGTGGTATCAACGCAGAG 6 0.11585248117397182 No Hit GCGTTGATACCACTGCTTATCCCAT 6 0.11585248117397182 No Hit ATACAGGGTGACAGCCCCGTACACA 6 0.11585248117397182 No Hit ACGCAGAGTACATGGGATAAGCAGT 6 0.11585248117397182 No Hit ACATGATAAGCAGTGGTATCAACGC 6 0.11585248117397182 No Hit GTACATGGGCAGTGGTATCAACGCA 6 0.11585248117397182 No Hit GTACTGGTTCACTATCGGTCAGTCA 6 0.11585248117397182 No Hit GCTTGTGTACTCTGCGTTGATACCA 6 0.11585248117397182 No Hit GCTTACATGTACTCTGCGTTGATAC 6 0.11585248117397182 No Hit CCATGTACTCTGCGTTGATACCACT 6 0.11585248117397182 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 95 1.0397648E-4 10.0 1 TACATAA 95 1.0397648E-4 10.0 2 ACATAAG 95 1.0397648E-4 10.0 3 TCAACGC 100 1.7506762E-4 9.5 18 CAACGCA 100 1.7506762E-4 9.5 19 TGGTATC 105 2.8675838E-4 9.04762 13 AGCAGTG 120 1.0323371E-4 8.708334 8 AAGCAGT 120 1.0323371E-4 8.708334 7 GGTATCA 110 4.5812025E-4 8.636364 14 CATAAGC 110 4.5812025E-4 8.636364 4 GCAGTGG 125 1.6137456E-4 8.36 9 TAAGCAG 115 7.154177E-4 8.260869 6 GTATCAA 115 7.154177E-4 8.260869 15 ATAAGCA 120 0.0010942043 7.916667 5 AGTGGTA 120 0.0010942043 7.916667 11 TATCAAC 120 0.0010942043 7.916667 16 GTGGTAT 120 0.0010942043 7.916667 12 ATCAACG 120 0.0010942043 7.916667 17 CAGTGGT 120 0.0010942043 7.916667 10 >>END_MODULE