FastQCFastQC Report
Wed 25 May 2016
SRR1295220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7414
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1401.8883193957377933No Hit
GCTTATGTACTCTGCGTTGATACCA961.2948475856487724No Hit
TATGTACTCTGCGTTGATACCACTG380.5125438359859725No Hit
CTTATGTACTCTGCGTTGATACCAC340.4585918532506069No Hit
GCGTTGATACCACTGCTTATGTACT310.4181278661990829No Hit
GAGTACATAAGCAGTGGTATCAACG310.4181278661990829No Hit
CCCATGTACTCTGCGTTGATACCAC300.4046398705152414No Hit
CATAAGCAGTGGTATCAACGCAGAG290.3911518748314No Hit
CTGCTTATGTACTCTGCGTTGATAC270.36417588346371726No Hit
GTACTGGTTCACTATCGGTCAGTCA220.2967359050445104No Hit
ACTCTGCGTTGATACCACTGCTTAT210.28324790936066896No Hit
CATGTACTCTGCGTTGATACCACTG200.2697599136768276No Hit
TATCAACGCAGAGTACATAAGCAGT190.25627191799298626No Hit
GTATCAACGCAGAGTACATAAGCAG170.22929592662530346No Hit
ACGCAGAGTACATAAGCAGTGGTAT170.22929592662530346No Hit
ATCATGTACTCTGCGTTGATACCAC170.22929592662530346No Hit
AAGCAGTGGTATCAACGCAGAGTAC150.2023199352576207No Hit
ACATAAGCAGTGGTATCAACGCAGA140.18883193957377933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA140.18883193957377933No Hit
GTACTCTGCGTTGATACCACTGCTT140.18883193957377933No Hit
GTACATGATAAGCAGTGGTATCAAC130.17534394388993796No Hit
GTACATGGGAAGCAGTGGTATCAAC120.16185594820609656No Hit
ATACCACTGCTTATGTACTCTGCGT120.16185594820609656No Hit
GTGGTATCAACGCAGAGTACATGGG120.16185594820609656No Hit
GTGGTATCAACGCAGAGTACATAAG120.16185594820609656No Hit
GGTATCAACGCAGAGTACATAAGCA100.1348799568384138No Hit
CCACTGCTTATGTACTCTGCGTTGA100.1348799568384138No Hit
CAGTGGTATCAACGCAGAGTACATA100.1348799568384138No Hit
GTATCAACGCAGAGTACTTTTTTTT100.1348799568384138No Hit
GCAGTGGTATCAACGCAGAGTACAT90.12139196115457243No Hit
GCTTATCCCATGTACTCTGCGTTGA90.12139196115457243No Hit
GATAAGCAGTGGTATCAACGCAGAG80.10790396547073104No Hit
GTATCAACGCAGAGTACATGGGAAG80.10790396547073104No Hit
GGTATCAACGCAGAGTACTTTTTTT80.10790396547073104No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)