##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7414 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.5168599946048 31.0 30.0 33.0 27.0 34.0 2 30.540463987051524 31.0 31.0 34.0 27.0 34.0 3 30.830186134340437 31.0 31.0 34.0 27.0 34.0 4 34.026841111410846 37.0 35.0 37.0 28.0 37.0 5 34.632721877529 37.0 35.0 37.0 32.0 37.0 6 34.75101159967629 37.0 35.0 37.0 32.0 37.0 7 34.62503371998921 37.0 35.0 37.0 31.0 37.0 8 34.66266522794713 37.0 35.0 37.0 32.0 37.0 9 35.789587267332074 39.0 35.0 39.0 30.0 39.0 10 35.76989479363367 39.0 35.0 39.0 30.0 39.0 11 36.16657674669544 39.0 37.0 39.0 31.0 39.0 12 36.25640679794982 39.0 37.0 39.0 31.0 39.0 13 36.04693822497977 39.0 37.0 39.0 30.0 39.0 14 36.86646884272997 40.0 37.0 41.0 29.0 41.0 15 37.270164553547346 40.0 37.0 41.0 31.0 41.0 16 37.180739142163475 40.0 37.0 41.0 31.0 41.0 17 36.991772322632855 40.0 37.0 41.0 30.0 41.0 18 36.905179390342596 40.0 37.0 41.0 30.0 41.0 19 37.28918262746156 40.0 37.0 41.0 31.0 41.0 20 37.28621526841111 40.0 37.0 41.0 31.0 41.0 21 37.24318856217966 40.0 37.0 41.0 31.0 41.0 22 37.21027785271109 40.0 37.0 41.0 30.0 41.0 23 37.040329107094685 40.0 37.0 41.0 30.0 41.0 24 37.18788777987591 40.0 37.0 41.0 30.0 41.0 25 36.16064202859455 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09104885414409125 1101 2 -1.0818689907975667 1101 3 -0.17897785635881291 1101 4 0.046982492815821786 1101 5 -2.101675264770499 1101 6 0.21354626414151312 1101 7 -0.9804974126402755 1101 8 -1.1448554972364562 1101 9 -1.1512740125835421 1101 10 -0.1988167035785935 1101 11 -0.14324784562879955 1101 12 0.1309881917024711 1101 13 1.2820346320346374 1101 14 -0.10169962312820502 1101 15 2.107280109661062 1101 16 0.015620776930290958 1101 17 0.8361290825576546 1101 18 -3.2407361818076126 1101 19 0.7311238364809824 1101 20 1.2614379888189475 1101 21 -3.325448427234136 1101 22 -0.14789106396248997 1101 23 -0.8730609998467145 1101 24 -0.7341095192285536 1101 25 1.4097655651227043 1102 1 -0.7577155208107591 1102 2 -0.30409121301978814 1102 3 0.3765776991967442 1102 4 -1.7307952849619568 1102 5 -0.10167526477049904 1102 6 0.5468795974748417 1102 7 -0.8693863015291612 1102 8 -0.8115221639031205 1102 9 -1.1512740125835421 1102 10 -1.7543722591341506 1102 11 -0.365470067851021 1102 12 0.575432636146914 1102 13 0.9487012987013017 1102 14 1.1205225990940235 1102 15 0.7739467763277332 1102 16 -0.8732681119585948 1102 17 -3.386093139664574 1102 18 -1.5740695151409483 1102 19 0.39779050314765385 1102 20 0.0392157665967261 1102 21 -0.5476706494563572 1102 22 0.7409978249263958 1102 23 -1.8730609998467145 1102 24 0.8214460363270035 1102 25 -1.0346788793217385 1103 1 0.9695572064619675 1103 2 0.5746966657680908 1103 3 0.8210221436411871 1103 4 0.8348612806946107 1103 5 1.3932742301789958 1103 6 0.9711220217172709 1103 7 0.9487955166526589 1103 8 1.3702960179150594 1103 9 2.3638775025679735 1103 10 2.2961327913709013 1103 11 2.0890753866944323 1103 12 1.9592710199853016 1103 13 1.8173881673881738 1103 14 2.8275933061647294 1103 15 2.4406134429943975 1103 16 2.116630877940395 1103 17 2.5431997896283605 1103 18 2.7592638181923874 1103 19 2.6503157556729064 1103 20 1.2816400090209683 1103 21 1.4523293505436428 1103 22 2.5187756027041743 1103 23 0.8643127375270225 1103 24 1.7608399757209412 1103 25 2.611785767142912 1104 1 -0.12135188444712242 1104 2 0.3928784839499073 1104 3 -0.4517051290860863 1104 4 -1.6196841738508425 1104 5 0.5750924119971756 1104 6 0.6983947489899975 1104 7 -0.14211357425643456 1104 8 -0.26606761844857374 1104 9 -5.09066795197748 1104 10 -0.5220490268109188 1104 11 0.8163481139671589 1104 12 1.231998292712575 1104 13 -1.0917027417027398 1104 14 0.8275933061647294 1104 15 0.8042498066307644 1104 16 1.4802672415767546 1104 17 2.088654335082907 1104 18 1.7592638181923874 1104 19 1.6503157556729064 1104 20 0.6452763726573352 1104 21 1.7250566232709161 1104 22 1.064230148158721 1104 23 1.5915854647997492 1104 24 1.4881127029936678 1104 25 3.1572403125974517 1105 1 -1.3940791571743958 1105 2 -0.5162124251410027 1105 3 -0.17897785635881291 1105 4 0.7439521897855244 1105 5 -3.061271224366461 1105 6 -0.5743325237372758 1105 7 -2.1421135742564346 1105 8 -1.4478858002667536 1105 9 -3.727031588341113 1105 10 -2.612958117720005 1105 11 -3.4563791587601145 1105 12 -2.586183525469245 1105 13 -4.728066378066373 1105 14 -2.5360430574716375 1105 15 -4.650295647914696 1105 16 -3.065187303877785 1105 17 -1.9113456649170928 1105 18 -4.058917999989433 1105 19 -3.0769569715998273 1105 20 -1.991087263706305 1105 21 -2.3658524676381774 1105 22 -2.753951670023099 1105 23 -4.1356872624729775 1105 24 -3.6027963879154257 1105 25 -4.2973051419480015 1106 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2212 2 0.44590878698021186 2212 3 0.9876888103078514 2212 4 1.3803158261491575 2212 5 1.453880290785058 2212 6 1.0468795974748417 2212 7 0.7972803651375031 2212 8 0.6884778360968795 2212 9 0.9320593207497936 2212 10 1.9400721853102922 2212 11 1.5511965988156433 2212 12 1.1865437472580282 2212 13 1.4764790764790803 2212 14 0.9260781546495735 2212 15 1.2739467763277332 2212 16 0.9878429991525124 2212 17 0.8916846381132046 2212 18 1.9259304848590517 2212 19 2.203346058703204 2212 20 -0.1274509000699382 2212 21 1.2023293505436428 2212 22 1.10210893603751 2212 23 1.2658278890421784 2212 24 1.9047793696603321 2212 25 0.3542100095671543 2213 1 1.780746017650781 2213 2 1.7215498126212374 2213 3 1.7440990667181069 2213 4 1.9187773646106976 2213 5 1.5051623420671092 2213 6 1.4186744692697175 2213 7 1.3613829292400723 2213 8 1.7269393745584196 2213 9 2.7205208592113337 2213 10 2.2541747494128614 2213 11 2.019145316764366 2213 12 1.3019283626426414 2213 13 1.8034021534021605 2213 14 0.5478730264444494 2213 15 1.9021519045328574 2213 16 1.7250224863320014 2213 17 2.955787202215774 2213 18 2.682340741269307 2213 19 0.5943716997288462 2213 20 0.3725490999300618 2213 21 1.6830985813128763 2213 22 1.6726217565503276 2213 23 2.0671099403242295 2213 24 2.2573434722244343 2213 25 -0.4791233237661814 2214 1 1.617284479189241 2214 2 0.966742120313544 2214 3 0.9460221436411871 2214 4 1.3178158261491575 2214 5 1.4955469574517224 2214 6 1.0677129308081774 2214 7 1.7556136984708388 2214 8 1.6884778360968795 2214 9 1.8695593207497936 2214 10 1.273405518643628 2214 11 1.384529932148979 2214 12 1.5615437472580282 2214 13 1.2264790764790803 2214 14 2.7594114879829093 2214 15 1.6906134429943975 2214 16 1.633676332485848 2214 17 1.3500179714465403 2214 18 2.0717638181923874 2214 19 0.7241793920365396 2214 20 1.7475490999300618 2214 21 1.3898293505436428 2214 22 1.4562756027041743 2214 23 1.2449945557088427 2214 24 0.6756127029936678 2214 25 -0.2916233237661814 2215 1 1.242284479189241 2215 2 0.6720992631706864 2215 3 1.6067364293554718 2215 4 1.4517443975777269 2215 5 1.2634041003088683 2215 6 1.4516415022367468 2215 7 1.4163279841851235 2215 8 1.6884778360968795 2215 9 1.0392021778926477 2215 10 0.45197694721505854 2215 11 0.2059585035775484 2215 12 -0.31345625274197175 2215 13 0.4407647907647956 2215 14 1.0808400594114786 2215 15 1.7977563001372516 2215 16 1.3568906182001328 2215 17 1.010732257160825 2215 18 1.4021209610495333 2215 19 -0.14189203653489102 2215 20 0.5868348142157771 2215 21 0.6666150648293581 2215 22 -1.2669386830101104 2215 23 0.6824945557088427 2215 24 1.2738269887079525 2215 25 2.235162390519534 2216 1 0.7422844791892409 2216 2 -0.07075787968645741 2216 3 0.7210221436411857 2216 4 1.480315826149159 2216 5 1.2205469574517238 2216 6 0.9802129308081788 2216 7 1.430613698470836 2216 8 1.088477836096878 2216 9 1.782059320749795 2216 10 1.7234055186436308 2216 11 1.9345299321489762 2216 12 1.2865437472580297 2216 13 1.5264790764790774 2216 14 1.8094114879829064 2216 15 1.7406134429943947 2216 16 1.571176332485848 2216 17 2.2250179714465403 2216 18 2.659263818192386 2216 19 1.5866793920365367 2216 20 0.4725490999300632 2216 21 -1.5476706494563572 2216 22 -1.8812243972958242 2216 23 -0.6175054442911545 2216 24 -0.9118872970063308 2216 25 -1.2791233237661785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 1.0 5 3.0 6 0.0 7 4.0 8 0.0 9 0.0 10 3.0 11 1.0 12 12.0 13 9.0 14 22.0 15 33.0 16 32.0 17 27.0 18 41.0 19 39.0 20 42.0 21 39.0 22 36.0 23 43.0 24 38.0 25 36.0 26 54.0 27 54.0 28 60.0 29 77.0 30 101.0 31 119.0 32 181.0 33 219.0 34 367.0 35 550.0 36 898.0 37 1417.0 38 2844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07992439584177 20.548130147158094 12.366680167409209 23.00526528959093 2 14.893904581700228 23.58426814434383 37.91052844979051 23.611298824165427 3 18.837052062204194 28.979039891818797 29.492900608519268 22.691007437457742 4 13.771645021645021 18.72294372294372 34.53733766233766 32.96807359307359 5 12.476319350473613 39.580514208389715 34.26251691474966 13.68064952638701 6 29.348120097376253 35.528807140925075 21.219908033540708 13.903164728157966 7 24.844468487963216 34.85258317554774 22.166621585068974 18.13632675142007 8 23.157040443662925 35.37129717300149 23.833355877181116 17.638306506154468 9 26.25456512917625 19.369674015961046 20.50588394427161 33.8698769105911 10 16.105020977128163 27.67627554472865 29.85519014751658 26.36351333062661 11 31.792376317923765 26.99378210327115 22.722357393890242 18.491484184914842 12 23.24134199134199 27.583874458874458 27.448593073593074 21.726190476190478 13 26.585530764029752 22.853279242731574 29.560513860716704 21.000676132521974 14 23.40770791075051 24.678837052062207 25.50371872887086 26.40973630831643 15 26.004600189419563 27.492896766337438 24.123934514950616 22.378568529292384 16 22.30935640886966 28.528934559221202 26.297998918334237 22.863710113574907 17 20.186713570558787 30.37478013800568 27.844675957245297 21.59383033419023 18 21.20925199513053 25.97051264709861 32.03029893142161 20.789936426349247 19 21.885567428648724 30.244826186933587 26.33572298119843 21.53388340321926 20 22.10953346855984 29.560513860716704 27.194050033806626 21.135902636916835 21 23.958333333333336 28.774350649350648 25.189393939393938 22.07792207792208 22 22.63385613845322 28.231476473769607 24.851270957274203 24.283396430502975 23 24.215792320173065 26.8388318009735 26.24391563007031 22.701460248783125 24 20.72230488299743 29.514405518733938 25.49709184363587 24.26619775463276 25 21.95319897200054 29.70377384011903 26.20045989449479 22.142567293385635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 25.0 2 21.0 3 17.0 4 17.0 5 17.0 6 23.0 7 29.0 8 29.0 9 29.0 10 30.0 11 31.0 12 31.0 13 31.0 14 43.0 15 55.0 16 55.0 17 55.0 18 74.5 19 94.0 20 94.0 21 94.0 22 145.0 23 196.0 24 196.0 25 196.0 26 246.0 27 296.0 28 296.0 29 296.0 30 372.0 31 448.0 32 448.0 33 448.0 34 527.5 35 607.0 36 607.0 37 607.0 38 686.5 39 766.0 40 766.0 41 766.0 42 1071.0 43 1376.0 44 1376.0 45 1376.0 46 1236.0 47 1096.0 48 1096.0 49 1096.0 50 950.0 51 804.0 52 804.0 53 804.0 54 679.0 55 554.0 56 554.0 57 554.0 58 468.0 59 382.0 60 382.0 61 382.0 62 334.5 63 287.0 64 287.0 65 287.0 66 225.0 67 163.0 68 163.0 69 163.0 70 130.0 71 97.0 72 97.0 73 97.0 74 73.5 75 50.0 76 50.0 77 50.0 78 36.0 79 22.0 80 22.0 81 22.0 82 17.0 83 12.0 84 12.0 85 12.0 86 8.5 87 5.0 88 5.0 89 5.0 90 3.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09441596978688967 2 0.2023199352576207 3 0.25627191799298626 4 0.2967359050445104 5 0.3237118964121931 6 0.2697599136768276 7 0.2697599136768276 8 0.28324790936066896 9 0.28324790936066896 10 0.33719989209603457 11 0.21580793094146208 12 0.2967359050445104 13 0.25627191799298626 14 0.25627191799298626 15 0.31022390072835176 16 0.24278392230914486 17 0.31022390072835176 18 0.28324790936066896 19 0.28324790936066896 20 0.25627191799298626 21 0.2967359050445104 22 0.24278392230914486 23 0.24278392230914486 24 0.28324790936066896 25 0.28324790936066896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7414.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.98570272457512 #Duplication Level Percentage of deduplicated Percentage of total 1 90.38592896174863 71.39196115457243 2 6.574453551912568 10.385756676557865 3 1.400273224043716 3.3180469382249798 4 0.426912568306011 1.3487995683841383 5 0.29030054644808745 1.1464796331265175 6 0.15368852459016394 0.7283517669274345 7 0.1878415300546448 1.0385756676557862 8 0.05122950819672131 0.3237118964121931 9 0.03415300546448088 0.24278392230914486 >10 0.4610655737704918 6.892365794442947 >50 0.01707650273224044 1.2948475856487724 >100 0.01707650273224044 1.8883193957377933 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 140 1.8883193957377933 No Hit GCTTATGTACTCTGCGTTGATACCA 96 1.2948475856487724 No Hit TATGTACTCTGCGTTGATACCACTG 38 0.5125438359859725 No Hit CTTATGTACTCTGCGTTGATACCAC 34 0.4585918532506069 No Hit GCGTTGATACCACTGCTTATGTACT 31 0.4181278661990829 No Hit GAGTACATAAGCAGTGGTATCAACG 31 0.4181278661990829 No Hit CCCATGTACTCTGCGTTGATACCAC 30 0.4046398705152414 No Hit CATAAGCAGTGGTATCAACGCAGAG 29 0.3911518748314 No Hit CTGCTTATGTACTCTGCGTTGATAC 27 0.36417588346371726 No Hit GTACTGGTTCACTATCGGTCAGTCA 22 0.2967359050445104 No Hit ACTCTGCGTTGATACCACTGCTTAT 21 0.28324790936066896 No Hit CATGTACTCTGCGTTGATACCACTG 20 0.2697599136768276 No Hit TATCAACGCAGAGTACATAAGCAGT 19 0.25627191799298626 No Hit GTATCAACGCAGAGTACATAAGCAG 17 0.22929592662530346 No Hit ACGCAGAGTACATAAGCAGTGGTAT 17 0.22929592662530346 No Hit ATCATGTACTCTGCGTTGATACCAC 17 0.22929592662530346 No Hit AAGCAGTGGTATCAACGCAGAGTAC 15 0.2023199352576207 No Hit ACATAAGCAGTGGTATCAACGCAGA 14 0.18883193957377933 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 14 0.18883193957377933 No Hit GTACTCTGCGTTGATACCACTGCTT 14 0.18883193957377933 No Hit GTACATGATAAGCAGTGGTATCAAC 13 0.17534394388993796 No Hit GTACATGGGAAGCAGTGGTATCAAC 12 0.16185594820609656 No Hit ATACCACTGCTTATGTACTCTGCGT 12 0.16185594820609656 No Hit GTGGTATCAACGCAGAGTACATGGG 12 0.16185594820609656 No Hit GTGGTATCAACGCAGAGTACATAAG 12 0.16185594820609656 No Hit GGTATCAACGCAGAGTACATAAGCA 10 0.1348799568384138 No Hit CCACTGCTTATGTACTCTGCGTTGA 10 0.1348799568384138 No Hit CAGTGGTATCAACGCAGAGTACATA 10 0.1348799568384138 No Hit GTATCAACGCAGAGTACTTTTTTTT 10 0.1348799568384138 No Hit GCAGTGGTATCAACGCAGAGTACAT 9 0.12139196115457243 No Hit GCTTATCCCATGTACTCTGCGTTGA 9 0.12139196115457243 No Hit GATAAGCAGTGGTATCAACGCAGAG 8 0.10790396547073104 No Hit GTATCAACGCAGAGTACATGGGAAG 8 0.10790396547073104 No Hit GGTATCAACGCAGAGTACTTTTTTT 8 0.10790396547073104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE