FastQCFastQC Report
Wed 25 May 2016
SRR1295220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7414
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1582.131103318046938No Hit
GCTTATGTACTCTGCGTTGATACCA801.0790396547073104No Hit
CTTATGTACTCTGCGTTGATACCAC620.8362557323981658No Hit
GTATCAACGCAGAGTACTTTTTTTT530.7148637712435931No Hit
TATGTACTCTGCGTTGATACCACTG380.5125438359859725No Hit
GAGTACATAAGCAGTGGTATCAACG300.4046398705152414No Hit
ACGCAGAGTACATAAGCAGTGGTAT290.3911518748314No Hit
GGTATCAACGCAGAGTACTTTTTTT280.37766387914755867No Hit
GCGTTGATACCACTGCTTATGTACT270.36417588346371726No Hit
TATCAACGCAGAGTACTTTTTTTTT260.3506878877798759No Hit
CCCATGTACTCTGCGTTGATACCAC240.3237118964121931No Hit
GTACATGATAAGCAGTGGTATCAAC220.2967359050445104No Hit
GGTATCAACGCAGAGTACATAAGCA210.28324790936066896No Hit
CTGCTTATGTACTCTGCGTTGATAC210.28324790936066896No Hit
CATGTACTCTGCGTTGATACCACTG210.28324790936066896No Hit
CATAAGCAGTGGTATCAACGCAGAG190.25627191799298626No Hit
ACTCTGCGTTGATACCACTGCTTAT180.24278392230914486No Hit
GTGGTATCAACGCAGAGTACATGGG180.24278392230914486No Hit
ATCATGTACTCTGCGTTGATACCAC160.21580793094146208No Hit
GTACTTTTTTTTTTTTTTTTTTTTT150.2023199352576207No Hit
AAGCAGTGGTATCAACGCAGAGTAC150.2023199352576207No Hit
TATCAACGCAGAGTACATAAGCAGT140.18883193957377933No Hit
GTGGTATCAACGCAGAGTACATAAG140.18883193957377933No Hit
CCACTGCTTATGTACTCTGCGTTGA130.17534394388993796No Hit
GTACTCTGCGTTGATACCACTGCTT120.16185594820609656No Hit
GCAGTGGTATCAACGCAGAGTACAT120.16185594820609656No Hit
GAGTACTTTTTTTTTTTTTTTTTTT120.16185594820609656No Hit
GTATCAACGCAGAGTACATAAGCAG110.1483679525222552No Hit
GGATACCACGTGTCCCGCCCTACTC100.1348799568384138No Hit
ATACCACTGCTTATGTACTCTGCGT100.1348799568384138No Hit
GCGTTGATACCACTGCTTATCATGT100.1348799568384138No Hit
TGATACCACTGCTTATGTACTCTGC100.1348799568384138No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100.1348799568384138No Hit
GTACATGGGATAAGCAGTGGTATCA90.12139196115457243No Hit
CCCATATTCAGACAGGATACCACGT90.12139196115457243No Hit
ACCCTGTATCGCGCGCCTTTCCAGA90.12139196115457243No Hit
ACATAAGCAGTGGTATCAACGCAGA80.10790396547073104No Hit
GAGTACATGATAAGCAGTGGTATCA80.10790396547073104No Hit
GGGATAAGCAGTGGTATCAACGCAG80.10790396547073104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA80.10790396547073104No Hit
GTACATGGGAAGCAGTGGTATCAAC80.10790396547073104No Hit
GTACTGGTTCACTATCGGTCAGTCA80.10790396547073104No Hit
GATACCACTGCTTATGTACTCTGCG80.10790396547073104No Hit
GTATCAACGCAGAGTACATGGGAAG80.10790396547073104No Hit
ATCATTAACTGAATCCATAGGTTAA80.10790396547073104No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)