##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295219_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8331 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.345096627055575 31.0 30.0 33.0 26.0 34.0 2 30.335854039130957 31.0 30.0 34.0 26.0 34.0 3 30.58204297203217 31.0 31.0 34.0 27.0 34.0 4 33.850318089064935 35.0 35.0 37.0 28.0 37.0 5 34.15868443164086 37.0 35.0 37.0 30.0 37.0 6 34.05677589725123 37.0 35.0 37.0 29.0 37.0 7 34.04417236826311 37.0 35.0 37.0 29.0 37.0 8 34.04549273796663 37.0 35.0 37.0 29.0 37.0 9 35.255911655263475 39.0 35.0 39.0 27.0 39.0 10 35.212219421438 39.0 35.0 39.0 27.0 39.0 11 35.707478093866285 39.0 35.0 39.0 30.0 39.0 12 35.66522626335374 39.0 35.0 39.0 29.0 39.0 13 35.577241627655745 39.0 35.0 39.0 29.0 39.0 14 36.34437642539911 40.0 36.0 41.0 27.0 41.0 15 36.67566918737246 40.0 36.0 41.0 27.0 41.0 16 36.649621894130355 40.0 36.0 41.0 28.0 41.0 17 36.32709158564398 39.0 36.0 41.0 27.0 41.0 18 36.21041891729684 39.0 36.0 41.0 27.0 41.0 19 36.611451206337776 40.0 36.0 41.0 27.0 41.0 20 36.55923658624415 40.0 36.0 41.0 27.0 41.0 21 36.662345456727884 40.0 36.0 41.0 28.0 41.0 22 36.487096386988355 40.0 36.0 41.0 27.0 41.0 23 36.28616012483496 39.0 36.0 41.0 27.0 41.0 24 36.63017644940583 40.0 36.0 41.0 28.0 41.0 25 35.3408954507262 39.0 34.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4249466453247983 1101 2 1.1916980894922098 1101 3 0.5867707455942757 1101 4 0.8550489504271042 1101 5 -1.535855651506914 1101 6 0.06714256409634345 1101 7 1.2741610746862833 1101 8 1.5339833252648205 1101 9 1.7056369556369546 1101 10 2.7647899835925003 1101 11 1.4281577976956186 1101 12 -0.24597058326050103 1101 13 0.17356385833276278 1101 14 1.4417757584774336 1101 15 1.4549772900613291 1101 16 0.03427990854461882 1101 17 0.4499700579532515 1101 18 2.366013853093669 1101 19 1.6872571678244057 1101 20 0.8205349894425638 1101 21 1.349007959827297 1101 22 1.9008537058957202 1101 23 -0.12126816149926611 1101 24 2.3370212226304687 1101 25 -1.6308085899472573 1102 1 -0.4607676403894878 1102 2 0.7202695180636383 1102 3 0.6867707455942771 1102 4 0.04076323614138744 1102 5 0.7784300627788028 1102 6 -0.47571457876080103 1102 7 -0.15441035388514734 1102 8 -2.480302389020892 1102 9 -2.108648758648762 1102 10 0.2219328407353558 1102 11 -0.500413630875812 1102 12 -0.8316848689747829 1102 13 -1.1550075702386664 1102 14 -0.7153670986654177 1102 15 -0.3164512813672431 1102 16 -0.10857723431252708 1102 17 0.492827200810396 1102 18 0.9660138530936706 1102 19 -1.6698856893184484 1102 20 -1.408036439128864 1102 21 0.0775793883987248 1102 22 0.4722822773242896 1102 23 -0.6641253043564106 1102 24 -1.7915502059409647 1102 25 -1.2736657328044032 1103 1 -0.25838668800853526 1103 2 0.20836475615887906 1103 3 0.35343741226094494 1103 4 -0.16161771623956156 1103 5 -0.019188984840248224 1103 6 -0.5828574359036551 1103 7 -0.642505591980381 1103 8 1.067316658598159 1103 9 -1.561029711029711 1103 10 -2.8018766830741626 1103 11 -2.5718422023043814 1103 12 -1.379303916593834 1103 13 -0.9764361416672358 1103 14 -0.7748909081892279 1103 15 -1.7450227099386737 1103 16 -0.13238675812204548 1103 17 -2.066696608713414 1103 18 -1.9506528135729937 1103 19 -0.8127428321755943 1103 20 -2.1461316772240977 1103 21 -0.9343253735060415 1103 22 -1.4324796274376155 1103 23 -3.0212681614992647 1103 24 -2.44631211070287 1103 25 -1.3808085899472573 1104 1 -1.175053354675203 1104 2 0.005983803777926511 1104 3 -0.38465782583429586 1104 4 0.04076323614138744 1104 5 0.2784300627788028 1104 6 0.16714256409634487 1104 7 0.20273250325771386 1104 8 1.0911261824076774 1104 9 -0.8943630443630468 1104 10 0.8647899835925017 1104 11 0.7138720834099033 1104 12 -1.688827726117644 1104 13 0.9164210011899101 1104 14 1.427490044191721 1104 15 1.183548718632757 1104 16 2.391422765687473 1104 17 1.0642557722389654 1104 18 0.6088709959508165 1104 19 0.11582859639583631 1104 20 -0.1223221534145793 1104 21 1.0775793883987248 1104 22 0.9008537058957202 1104 23 -0.6641253043564106 1104 24 0.1370212226304659 1104 25 -1.1308085899472573 1105 1 -0.02120720082904981 1105 2 -0.2083019105077888 1105 3 0.2252322840558172 1105 4 -0.12956643418828406 1105 5 -0.16662488227614602 1105 6 -1.6020882051344216 1105 7 -0.34122354069832994 1105 8 -3.5352474439659467 1105 9 -5.0482091982092 1105 10 -2.6736715548690384 1105 11 -1.6487652792274616 1105 12 -2.853662890952805 1105 13 -1.6110515262826226 1105 14 -0.7813011645994834 1105 15 -0.8604073253232869 1105 16 -2.927258552993841 1105 17 -0.8423376343544433 1105 18 0.2737061607859772 1105 19 -0.4665889860217476 1105 20 -0.4794650105574334 1105 21 -5.812530501711166 1105 22 -4.176069371027356 1105 23 -0.8674220076531114 1105 24 -0.2091326235233808 1105 25 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1.2397437024537865 1107 13 1.8449924297613336 1107 14 2.927490044191721 1107 15 2.8264058614898957 1107 16 2.3199941942589035 1107 17 1.9213986293818195 1107 18 2.680299567379386 1107 19 3.258685739252975 1107 20 2.1633921322997125 1107 21 2.7204365312558636 1107 22 3.4722822773242896 1107 23 3.121588981357881 1107 24 2.7084497940590353 1107 25 1.083477124338458 1108 1 -0.9607676403894878 1108 2 -0.4225876247935041 1108 3 -0.8846578258342959 1108 4 -1.9592367638586126 1108 5 0.3498586342073722 1108 6 0.23857113552491427 1108 7 1.4884467889719986 1108 8 1.162554753836254 1108 9 -0.7515059015059009 1108 10 0.007647126449647601 1108 11 -2.000413630875812 1108 12 0.3826008453109324 1108 13 0.0592781440470489 1108 14 -0.429652812951133 1108 15 -1.1021655670815278 1108 16 -0.10857723431252708 1108 17 -2.2214585134753193 1108 18 -2.319700432620614 1108 19 -2.098457117889879 1108 20 -0.9794650105574334 1108 21 -1.3509920401727058 1108 22 -0.6705748655328492 1108 23 -0.3069824472135494 1108 24 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3.101088240268915 1110 13 3.126505034803351 1110 14 3.8476581114186104 1110 15 3.0490949371201523 1110 16 3.063691673250503 1110 17 3.085264175600308 1110 18 3.7307197354466126 1110 19 3.6284336384126377 1110 20 3.4028879306190376 1110 21 3.354890312768468 1110 22 3.1949713529545463 1110 23 2.2728494855595613 1110 24 1.5781976932186979 1110 25 3.0162502335821557 1111 1 -0.1750533546752031 1111 2 1.4345552323493536 1111 3 1.5439136027371347 1111 4 1.3264775218556721 1111 5 -0.2929985086497666 1111 6 1.024285421239199 1111 7 0.13130393182913735 1111 8 1.019697610979108 1111 9 1.748494098494099 1111 10 1.2933614121639323 1111 11 1.6424435119813339 1111 12 1.7397437024537865 1111 13 -0.6550075702386664 1111 14 1.427490044191721 1111 15 1.8264058614898957 1111 16 1.0342799085446188 1111 17 2.207112915096104 1111 18 1.3231567102365247 1111 19 2.3301143106815516 1111 20 2.591963560871136 1111 21 2.077579388398725 1111 22 2.329425134467151 1111 23 1.76444612421502 1111 24 0.99416407977332 1111 25 -0.9879514470901114 1112 1 1.6249466453247976 1112 2 1.1250314228255434 1112 3 0.6867707455942771 1112 4 2.1883822837604328 1112 5 1.530811015159756 1112 6 2.4338092307630106 1112 7 2.540827741352949 1112 8 2.1006499919314905 1112 9 1.5056369556369518 1112 10 0.9314566502591717 1112 11 -0.3051755356377157 1112 12 1.0540294167395032 1112 13 0.1068971916660999 1112 14 0.27510909181076926 1112 15 1.7549772900613263 1112 16 2.200946575211283 1112 17 1.1833033912865858 1112 18 1.566013853093672 1112 19 2.7539238344910757 1112 20 2.3205349894425638 1112 21 1.1156746264939628 1112 22 1.100853705895723 1112 23 -0.15460149483259755 1112 24 1.9703545559638016 1112 25 3.102524743386077 1113 1 -1.2417200213418695 1113 2 -0.14163524384112236 1113 3 -0.8465625877390579 1113 4 1.1217156170937699 1113 5 -0.8025223181735797 1113 6 -1.3661907692369866 1113 7 -0.5258389253137139 1113 8 -2.966016674735176 1113 9 -1.2943630443630454 1113 10 -2.0018766830741654 1113 11 -0.43850886897104857 1113 12 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2.0741610746862804 2206 8 1.3339833252648248 2206 9 3.7056369556369546 2206 10 3.464789983592503 2206 11 2.6281577976956214 2206 12 2.6540294167395047 2206 13 2.773563858332764 2206 14 3.4417757584774336 2206 15 1.8549772900613277 2206 16 3.034279908544619 2206 17 3.2499700579532487 2206 18 3.866013853093669 2206 19 3.6872571678244057 2206 20 3.920534989442565 2206 21 1.4490079598272914 2206 22 3.5008537058957216 2206 23 0.6787318385007381 2206 24 2.5370212226304645 2206 25 4.269191410052741 2207 1 0.09161331199146261 2207 2 -0.0083019105077895 2207 3 -0.7132292544057215 2207 4 -0.7449510495728973 2207 5 -0.9358556515069125 2207 6 -0.43285743590365655 2207 7 -0.1925055919803853 2207 8 0.3673166585981562 2207 9 -1.0943630443630497 2207 10 -2.601876683074167 2207 11 0.02815779769561999 2207 12 1.120696083406166 2207 13 -1.026436141667233 2207 14 -0.9915575748558965 2207 15 -1.3783560432720066 2207 16 0.40094657521128596 2207 17 -2.6166966087134185 2207 18 -1.500652813572998 2207 19 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0.6671425640963449 2209 7 0.7741610746862833 2209 8 0.9482690395505387 2209 9 0.8199226699226685 2209 10 -1.7066385878360677 2209 11 -1.8575564880186661 2209 12 0.3111722738823559 2209 13 0.7735638583327642 2209 14 0.3560614727631517 2209 15 -0.6021655670815278 2209 16 -0.7514343771696659 2209 17 -0.8643156563324652 2209 18 -0.96255757547776 2209 19 -1.8841714036041637 2209 20 0.7348207037282819 2209 21 0.5775793883987248 2209 22 -0.8848605798185645 2209 23 -1.0212681614992647 2209 24 1.9227355083447506 2209 25 2.8691914100527427 2210 1 -1.0981302777521265 2210 2 -1.9775326797385588 2210 3 -0.7747677159441828 2210 4 -0.8218741264959775 2210 5 -2.012778728429989 2210 6 -0.9097805128267353 2210 7 -1.87968507915987 2210 8 -0.5352474439659431 2210 9 -0.5866707366707402 2210 10 0.0955592143617352 2210 11 -1.1103037407659215 2210 12 -0.469047506337418 2210 13 -1.303359218590316 2210 14 -1.4736088569071768 2210 15 -0.7065611714771336 2210 16 -0.15802778376307458 2210 17 -0.5346453266621367 2210 18 -3.418601531521716 2210 19 -1.697358216790981 2210 20 -3.32561885671128 2210 21 -0.5048381940188591 2210 22 -0.09914629410427978 2210 23 -0.5597296999608048 2210 24 -0.5937480081387676 2210 25 -2.3231162822549507 2211 1 0.39637521675336984 2211 2 -1.0654447676506464 2211 3 0.25819931702285004 2211 4 -0.530665335287182 2211 5 -0.6501413657926278 2211 6 0.3099997069534908 2211 7 -0.5115532110280014 2211 8 -0.19458810330660725 2211 9 -1.0372201872201927 2211 10 -0.2066385878360677 2211 11 -0.07184220230438143 2211 12 -1.9745420118319288 2211 13 0.4164210011899101 2211 14 0.7132043299060058 2211 15 -0.959308424224389 2211 16 0.4628513371160494 2211 17 0.8499700579532501 2211 18 -0.46255757547776 2211 19 1.258685739252975 2211 20 0.8062492751568513 2211 21 0.5061508169701483 2211 22 -1.0277177226757104 2211 23 0.6215889813578812 2211 24 1.2798783654876118 2211 25 1.1549056957670274 2212 1 -1.4607676403894878 2212 2 -3.351159053364931 2212 3 -1.813229254405723 2212 4 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-0.4657200914553812 2213 17 2.1576623656455567 2213 18 -0.033986146906329395 2213 19 1.841103321670559 2213 20 1.3666888355964133 2213 21 0.4951618059811409 2213 22 0.5162383212803334 2213 23 0.4402703000391952 2213 24 0.7139442995535461 2213 25 1.2922683331296625 2214 1 -0.1750533546752031 2214 2 1.3631266609207842 2214 3 1.4724850313085618 2214 4 -0.8163796210014667 2214 5 1.7070014913502334 2214 6 1.9528568498106296 2214 7 -0.5829817824565708 2214 8 0.16255475383625395 2214 9 0.3199226699226685 2214 10 -1.7066385878360677 2214 11 -1.3575564880186661 2214 12 -0.2602562975462135 2214 13 1.7021352869041948 2214 14 0.7132043299060058 2214 15 0.3264058614898957 2214 16 1.3199941942589035 2214 17 1.0642557722389654 2214 18 0.2517281388079553 2214 19 0.5444000249672598 2214 20 -0.1937507248431487 2214 21 0.1490079598272942 2214 22 1.4722822773242896 2214 23 1.407303267072166 2214 24 1.9227355083447506 2214 25 -0.7022371613758267 2215 1 -0.5868180605575546 2215 2 -0.6200666163901403 2215 3 -1.842641019111607 2215 4 -2.0390686966317197 2215 5 -0.11232623974220957 2215 6 -0.009328024138952173 2215 7 0.5682787217451093 2215 8 0.6457480311471784 2215 9 0.5762251909310692 2215 10 0.6883193953572047 2215 11 -0.9247833787749684 2215 12 -2.016558818554614 2215 13 -1.8734949651966488 2215 14 -1.0346948297578606 2215 15 -3.0685521217033767 2215 16 -4.112778914984794 2215 17 -1.679441706752634 2215 18 -1.1516332057298584 2215 19 -0.0774487145285363 2215 20 -2.4794650105574334 2215 21 -0.4686390989962348 2215 22 -1.1579698235160478 2215 23 0.39049654438309034 2215 24 -1.8923905420754181 2215 25 -1.4543380017119603 2216 1 -0.5083866880085353 2216 2 0.45836475615887906 2216 3 0.020104078927609237 2216 4 -1.578284382906233 2216 5 -0.6025223181735768 2216 6 0.5004758974296806 2216 7 0.27416107468628326 2216 8 0.817316658598159 2216 9 0.5223036223036175 2216 10 2.1147899835925017 2216 11 0.09482446436228287 2216 12 0.2040294167395018 2216 13 0.6902305249994285 2216 14 -0.9415575748558993 2216 15 0.088310623394662 2216 16 0.4509465752112831 2216 17 1.9333033912865858 2216 18 0.7160138530936706 2216 19 -0.22940949884225859 2216 20 -0.7294650105574334 2216 21 0.9823412931606299 2216 22 1.9008537058957202 2216 23 -0.1046014948326004 2216 24 -1.7796454440361984 2216 25 0.2025247433860784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 1.0 4 1.0 5 2.0 6 2.0 7 2.0 8 3.0 9 1.0 10 5.0 11 12.0 12 20.0 13 25.0 14 22.0 15 45.0 16 46.0 17 50.0 18 45.0 19 51.0 20 40.0 21 51.0 22 72.0 23 48.0 24 58.0 25 63.0 26 70.0 27 85.0 28 82.0 29 128.0 30 147.0 31 171.0 32 215.0 33 309.0 34 433.0 35 662.0 36 921.0 37 1498.0 38 2930.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53143406659454 21.396802500300517 14.052169731938935 23.019593701166006 2 15.705861114454207 21.133710434468647 39.475267781923215 23.68516066915393 3 17.712221553281157 25.586995785671284 31.860325105358218 24.840457555689344 4 13.007346742141396 17.52378658316271 37.829700108394555 31.639166566301334 5 12.764932562620423 37.560211946050096 35.41666666666667 14.258188824662813 6 30.230037335902686 38.38371672889317 17.632181139347225 13.754064795856918 7 25.5088522220884 32.060700951463325 24.18402986872215 18.246416957726122 8 24.02745995423341 36.20378176562688 21.980007226303748 17.788751053835963 9 24.313583815028903 16.847302504816955 22.483140655105974 36.35597302504817 10 15.108433734939759 30.481927710843376 33.71084337349398 20.698795180722893 11 32.14543703346978 25.88490248013484 23.47700457500602 18.49265591138936 12 20.72495183044316 27.32418111753372 31.900289017341038 20.050578034682083 13 26.46279797736576 23.86226823982663 27.076811943173613 22.598121839634 14 19.86276634163958 23.05284699650897 28.38569880823402 28.698687853617432 15 22.22757375075256 31.354605659241418 23.853100541842267 22.564720048163757 16 22.18610810160106 31.515589262068133 25.07523775129409 21.223064885036717 17 19.898819561551434 30.402312695735965 28.872560828715972 20.82630691399663 18 20.749036608863197 29.684489402697494 29.564065510597302 20.002408477842003 19 21.613485851896446 28.741721854304636 28.862131246237205 20.78266104756171 20 22.096521843783847 28.27055000601757 29.23336141533277 20.39956673486581 21 22.988921001926784 28.468208092485547 27.769749518304433 20.773121387283236 22 21.593644679826674 27.660086663456905 29.465575349061147 21.28069330765527 23 22.418772563176894 28.592057761732853 27.749699157641395 21.239470517448858 24 21.68573148705599 27.971101745936185 29.06682721252258 21.27633955448525 25 22.652864708714493 28.41839191141069 27.51564756860857 21.41309581126625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24.0 1 24.0 2 18.5 3 13.0 4 13.0 5 13.0 6 15.5 7 18.0 8 18.0 9 18.0 10 22.0 11 26.0 12 26.0 13 26.0 14 48.0 15 70.0 16 70.0 17 70.0 18 112.5 19 155.0 20 155.0 21 155.0 22 235.0 23 315.0 24 315.0 25 315.0 26 384.0 27 453.0 28 453.0 29 453.0 30 587.0 31 721.0 32 721.0 33 721.0 34 792.5 35 864.0 36 864.0 37 864.0 38 934.5 39 1005.0 40 1005.0 41 1005.0 42 1024.0 43 1043.0 44 1043.0 45 1043.0 46 990.5 47 938.0 48 938.0 49 938.0 50 875.0 51 812.0 52 812.0 53 812.0 54 748.0 55 684.0 56 684.0 57 684.0 58 597.5 59 511.0 60 511.0 61 511.0 62 430.0 63 349.0 64 349.0 65 349.0 66 264.0 67 179.0 68 179.0 69 179.0 70 136.5 71 94.0 72 94.0 73 94.0 74 65.0 75 36.0 76 36.0 77 36.0 78 26.5 79 17.0 80 17.0 81 17.0 82 9.5 83 2.0 84 2.0 85 2.0 86 2.0 87 2.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14404033129276198 2 0.26407394070339696 3 0.31208738446765094 4 0.33609410634977793 5 0.32409074540871446 6 0.33609410634977793 7 0.33609410634977793 8 0.33609410634977793 9 0.32409074540871446 10 0.37210418917296845 11 0.3000840235265874 12 0.32409074540871446 13 0.3000840235265874 14 0.28808066258552395 15 0.31208738446765094 16 0.28808066258552395 17 0.34809746729084146 18 0.32409074540871446 19 0.31208738446765094 20 0.26407394070339696 21 0.32409074540871446 22 0.2760773016444605 23 0.25207057976233344 24 0.31208738446765094 25 0.2760773016444605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 8331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.1634857760173 #Duplication Level Percentage of deduplicated Percentage of total 1 98.85057471264368 97.03516984755731 2 0.9537784299339692 1.8725243068059056 3 0.07336757153338225 0.21606049693914295 4 0.024455857177794083 0.09602688752850798 5 0.036683785766691124 0.18005041411595246 6 0.0 0.0 7 0.0 0.0 8 0.024455857177794083 0.19205377505701596 9 0.0 0.0 >10 0.036683785766691124 0.4081142719961589 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 13 0.15604369223382547 No Hit GTATCAACGCAGAGTACTTTTTTTT 11 0.13203697035169848 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 10 0.12003360941063498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE