FastQCFastQC Report
Wed 25 May 2016
SRR1295219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240.28808066258552395No Hit
GGTATCAACGCAGAGTACTTTTTTT230.2760773016444605No Hit
TATCAACGCAGAGTACTTTTTTTTT130.15604369223382547No Hit
GTGGTATCAACGCAGAGTACATGGG110.13203697035169848No Hit
GGTATCAACGCAGAGTACATGGGGG100.12003360941063498No Hit
GAATGGAATGGAATGGAATGGAATG100.12003360941063498No Hit
ATTCCATTCCATTCCATTCCATTCC90.10803024846957147No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90.10803024846957147No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)