##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8331 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.424558876485417 31.0 31.0 33.0 28.0 34.0 2 31.441963749849958 31.0 31.0 34.0 28.0 34.0 3 31.641819709518664 31.0 31.0 34.0 28.0 34.0 4 35.25351098307526 37.0 35.0 37.0 32.0 37.0 5 35.38902892809987 37.0 35.0 37.0 33.0 37.0 6 35.29300204057136 37.0 35.0 37.0 33.0 37.0 7 35.20669787540511 37.0 35.0 37.0 32.0 37.0 8 35.186892329852355 37.0 35.0 37.0 32.0 37.0 9 36.92053775057016 39.0 37.0 39.0 33.0 39.0 10 36.95894850558156 39.0 37.0 39.0 33.0 39.0 11 36.967230824630896 39.0 37.0 39.0 33.0 39.0 12 36.89028928099868 39.0 37.0 39.0 33.0 39.0 13 36.79090145240667 39.0 37.0 39.0 32.0 39.0 14 38.060737006361784 40.0 38.0 41.0 33.0 41.0 15 38.07214019925579 40.0 38.0 41.0 33.0 41.0 16 38.066978754051135 40.0 38.0 41.0 33.0 41.0 17 38.092065778417954 40.0 38.0 41.0 33.0 41.0 18 38.099267794982595 40.0 38.0 41.0 33.0 41.0 19 38.19889569079342 40.0 38.0 41.0 33.0 41.0 20 38.16528628015845 40.0 38.0 41.0 33.0 41.0 21 38.08858480374505 40.0 38.0 41.0 33.0 41.0 22 38.04321209938783 40.0 38.0 41.0 33.0 41.0 23 38.03673028447965 40.0 38.0 41.0 32.0 41.0 24 38.023766654663305 40.0 38.0 41.0 33.0 41.0 25 36.94898571600048 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15831299497267537 1101 2 0.5810747570201293 1101 3 0.4584033069327198 1101 4 -0.4991005570837572 1101 5 -0.25481604452193096 1101 6 0.6064440539230347 1101 7 0.8472049386965423 1101 8 0.4636672384571625 1101 9 0.3508981137132423 1101 10 1.1270570699142155 1101 11 0.09607398903617792 1101 12 0.1130900456530668 1101 13 1.5249272824903102 1101 14 0.9833821174157151 1101 15 0.5903554273302092 1101 16 0.6833681335782344 1101 17 1.2482590316623998 1101 18 0.4284473385313845 1101 19 0.6080366451164707 1101 20 0.4938309822763429 1101 21 1.4671282071492087 1101 22 -0.9317239366609087 1101 23 -2.2486294051420117 1101 24 -1.6398343253385335 1101 25 -2.522660688206905 1102 1 -0.07259870925838996 1102 2 -0.04749667155130055 1102 3 0.5441175926470017 1102 4 0.8437565857733915 1102 5 0.0023268126209217144 1102 6 -0.05069880321981657 1102 7 -0.15279506130345766 1102 8 0.37795295274288065 1102 9 0.5223266851418131 1102 10 0.5556284984856461 1102 11 0.3103597033218932 1102 12 0.2988043313673501 1102 13 0.23921299677602548 1102 14 -1.3023321682985696 1102 15 -0.46678742981264776 1102 16 -1.802346152136053 1102 17 0.10540188880525392 1102 18 -0.10012409004004752 1102 19 0.06517950225932623 1102 20 0.6509738394191942 1102 21 0.25284249286349336 1102 22 -1.8174382223751948 1102 23 0.47994202342941605 1102 24 0.21730853180432064 1102 25 1.648767883221666 1103 1 -0.35831299497267466 1103 2 -0.03559190964654135 1103 3 0.6750699735993813 1103 4 0.7842327762495813 1103 5 0.3118506221447319 1103 6 0.8064440539230375 1103 7 0.930538272029878 1103 8 0.5803339051238297 1103 9 0.867564780379908 1103 10 1.3770570699142155 1103 11 1.596073989036178 1103 12 1.4297567123197297 1103 13 1.0249272824903102 1103 14 1.9833821174157151 1103 15 2.3903554273302063 1103 16 0.9000348002448959 1103 17 2.164925698329064 1103 18 1.828447338531383 1103 19 2.1247033117831364 1103 20 2.377164315609676 1103 21 1.8004615404825444 1103 22 1.4682760633390899 1103 23 0.9680372615246498 1103 24 0.19349900799480224 1103 25 -0.005994021540239203 1104 1 0.14168700502732534 1104 2 0.23821761416298415 1104 3 0.9012604497898629 1104 4 0.4866137286305303 1104 5 0.07375538404949822 1104 6 1.0207297682087528 1104 7 0.8472049386965423 1104 8 0.8779529527428807 1104 9 1.0937552565703896 1104 10 1.4841999270570696 1104 11 1.3817882747504626 1104 12 0.7988043313673501 1104 13 -0.4750727175096898 1104 14 -0.8023321682985696 1104 15 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13 -1.2607870032239745 1107 14 0.7690964031299998 1107 15 -0.7525017155269396 1107 16 -1.302346152136053 1107 17 -0.7517409683376002 1107 18 -1.171552661468617 1107 19 1.0651795022593262 1107 20 0.8652595537049095 1107 21 0.18141392143492396 1107 22 0.6111332061962358 1107 23 0.05137059485798545 1107 24 1.0744513889471818 1107 25 0.0773393117930965 1108 1 0.6416870050273253 1108 2 0.0953604713058418 1108 3 0.47268902121843226 1108 4 0.7008994429162456 1108 5 0.9308982411923523 1108 6 0.7350154824944681 1108 7 1.0614906529822576 1108 8 0.5208100956000195 1108 9 0.23661239942752843 1108 10 0.6270570699142155 1108 11 0.024645417607608522 1108 12 1.3702329027959195 1108 13 1.0249272824903102 1108 14 2.0548106888442845 1108 15 1.5332125701873522 1108 16 1.2690824192925163 1108 17 1.105401888805254 1108 18 -0.17155266146861692 1108 19 0.5651795022593262 1108 20 1.29383098227634 1108 21 1.1099853500063546 1108 22 0.4682760633390899 1108 23 1.7656563091437008 1108 24 1.6458799603757512 1108 25 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-0.5996556625758345 2208 20 -0.2830920946467401 2208 21 1.0440512840722889 2208 22 -0.3394162443532167 2208 23 -1.0255524820650948 2208 24 -1.7552189407231467 2208 25 0.4619546964084833 2209 1 0.9988298621701794 2209 2 0.7382176141629806 2209 3 0.9726890212184323 2209 4 1.1294708714876762 2209 5 0.9308982411923523 2209 6 0.02072976820875283 2209 7 0.7757763672679729 2209 8 0.6636672384571654 2209 9 -0.47767331485818687 2209 10 0.8413427841999308 2209 11 1.596073989036178 2209 12 0.7273757599387807 2209 13 0.7392129967760255 2209 14 0.41195354598714573 2209 15 0.676069713044491 2209 16 0.8405109907210857 2209 17 0.10540188880525392 2209 18 0.971304481388529 2209 19 0.850893787973618 2209 20 1.5081166965620554 2209 21 0.8242710642920628 2209 22 1.2539903490533746 2209 23 0.9085134520008395 2209 24 1.0744513889471818 2209 25 0.7916250260788118 2210 1 0.10322546656578524 2210 2 -0.6958483199029502 2210 3 -0.1262120776826663 2210 4 0.7778225198393258 2210 5 0.5682608785549874 2210 6 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1.828447338531383 2211 19 0.49375093083075683 2211 20 2.0081166965620554 2211 21 1.7528424928634934 2211 22 1.8968474919105205 2211 23 1.8370848805722702 2211 24 2.074451388947182 2211 25 2.648767883221666 2212 1 -1.501170137829817 2212 2 -0.04749667155130055 2212 3 0.04411759264700166 2212 4 0.3437565857733915 2212 5 0.2880410983352135 2212 6 0.30644405392303753 2212 7 -1.7956522041606036 2212 8 -0.33633276154283465 2212 9 -0.7633876005724716 2212 10 -0.0872286443714998 2212 11 -0.9039260109638221 2212 12 -1.20119566863265 2212 13 -0.3322155746525439 2212 14 -0.30233216829856957 2212 15 -0.39535885838407836 2212 16 -2.3737747235646225 2212 17 -1.1088838254804543 2212 18 -2.671552661468617 2212 19 -1.7919633548835279 2212 20 -1.20616901772366 2212 21 0.7528424928634934 2212 22 -0.03172393666091011 2212 23 -0.44862940514201455 2212 24 0.5030228175186124 2212 25 1.148767883221666 2213 1 0.7955331588734786 2213 2 -0.23430985836449025 2213 3 -0.3569813084518998 2213 4 -0.3760236340068346 2213 5 -0.047123736829625784 2213 6 -1.424325176846196 2213 7 -0.9220258305342242 2213 8 0.048282623072552155 2213 9 -1.6644865016713695 2213 10 -0.026789083931937796 2213 11 -1.2885413955792089 2213 12 -0.3715253389623214 2213 13 -1.1289188713558431 2213 14 -1.0166178825842849 2213 15 -2.340413803439027 2213 16 -0.1704780202679217 2213 17 -0.020971737568366677 2213 18 -0.01770650762246362 2213 19 0.6311135681933919 2213 20 -0.3600151715698132 2213 21 -1.340564100543098 2213 22 -0.3394162443532167 2213 23 -0.8717063282189343 2213 24 -0.13983432533853346 2213 25 1.1542623887161767 2214 1 -0.2868844235441017 2214 2 -0.47606810012273115 2214 3 -0.8844538359244254 2214 4 -0.5848148427980391 2214 5 -2.8548160445219324 2214 6 -1.5506988032198166 2214 7 -2.5099379184463118 2214 8 -4.122047047257119 2214 9 -3.1205304577153257 2214 10 -3.944371501514354 2214 11 -3.403926010963822 2214 12 -2.9869099543469346 2214 13 -3.0465012889382592 2214 14 -3.4451893111557155 2214 15 -1.4667874298126478 2214 16 -1.6594890092789143 2214 17 -3.2517409683376 2214 18 -3.885838375754332 2214 19 -4.791963354883528 2214 20 -3.70616901772366 2214 21 -4.818586078565076 2214 22 -7.1745810795180525 2214 23 -1.9486294051420145 2214 24 -1.3541200396242488 2214 25 -3.351232116778334 2215 1 0.28874582855673836 2215 2 0.1457806393730685 2215 3 -0.38865551659669606 2215 4 1.0538406193868326 2215 5 1.0569486613604226 2215 6 -0.5464971225475495 2215 7 -0.5057362377740446 2215 8 0.19307900316304938 2215 9 0.06854517253677272 2215 10 0.7741158934436285 2215 11 0.7431328125655909 2215 12 0.8366194574177683 2215 13 0.14257434131383917 2215 14 -1.075441411996053 2215 15 -0.5802328079639096 2215 16 0.8657210747547026 2215 17 -0.8987997918670132 2215 18 -1.1127291320568489 2215 19 0.8256837039400011 2215 20 -0.20616901772365992 2215 21 0.32006938361979564 2215 22 -0.03172393666091011 2215 23 -0.8309823463184856 2215 24 -2.8457166782797074 2215 25 1.1361628412048645 2216 1 0.6416870050273253 2216 2 0.7977414236867908 2216 3 0.008403306932716958 2216 4 0.6175661095829099 2216 5 -1.0214827111885967 2216 6 -1.1935559460769625 2216 7 -0.7361283946367934 2216 8 -0.9196660948761703 2216 9 -0.7991018862867563 2216 10 -0.2896095967524488 2216 11 -1.3205926776304864 2216 12 -1.903576621013599 2216 13 0.4415939491569745 2216 14 -0.26661788258428487 2216 15 -0.27631123933645796 2216 16 0.9000348002448959 2216 17 -0.16840763500426448 2216 18 0.3284473385313831 2216 19 1.5413699784498078 2216 20 -0.3728356843903242 2216 21 -0.5328717928507913 2216 22 -1.5317239366609101 2216 23 -1.7819627384753502 2216 24 1.1101656746614665 2216 25 -1.5893273548735678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 4.0 14 4.0 15 10.0 16 15.0 17 22.0 18 24.0 19 12.0 20 21.0 21 25.0 22 30.0 23 27.0 24 37.0 25 36.0 26 38.0 27 40.0 28 53.0 29 73.0 30 117.0 31 146.0 32 181.0 33 258.0 34 372.0 35 519.0 36 877.0 37 1508.0 38 3879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.7406962785114 21.48859543817527 12.797118847539016 23.973589435774308 2 15.508342335854039 21.522026167326853 38.81886928339935 24.150762213419757 3 17.392870003601008 26.7674948985716 30.164446044892568 25.675189052934822 4 12.4954987396471 18.56919937582523 37.33045252670748 31.604849357820193 5 13.458998679313241 38.347940929283226 34.349861928202664 13.843198463200865 6 31.376785499939984 38.326731484815745 17.18881286760293 13.10767014764134 7 26.107310046813108 32.42107790181251 23.010442924018726 18.46116912735566 8 23.7426479414236 36.394190373304525 21.353979114151965 18.509182571119915 9 24.174768935301884 16.792701956547834 22.17020765814428 36.862321450006 10 14.356019685511942 30.056415796422996 34.497659344616494 21.089905173448564 11 32.12099387828592 25.207057976233344 23.922698355539552 18.74924978994118 12 21.666066498619614 27.343656223742645 31.18473172488297 19.805545552754772 13 26.983555395510745 23.706637858600406 26.719481454807347 22.590325291081502 14 20.285679990397313 22.878405953667027 28.628015844436444 28.20789821149922 15 22.78237906613852 30.968671227943823 24.46284959788741 21.786100108030247 16 22.314247989437042 30.5485535950066 26.167326851518425 20.96987156403793 17 19.433441363581803 30.224462849597888 29.636298163485776 20.705797623334536 18 20.14163965910455 29.624294802544714 30.068419157364062 20.165646380986676 19 20.59776737486496 27.991837714560074 30.992677949825953 20.41771696074901 20 22.506301764494058 28.123874684911776 28.83207298043452 20.537750570159645 21 23.526587444484456 28.94010322890409 27.295642779978397 20.237666546633058 22 21.11391189533069 28.183891489617093 29.204177169607494 21.498019445444726 23 21.82211019085344 28.652022566318568 28.1478814067939 21.37798583603409 24 21.24594886568239 27.58372344256392 30.344496459008525 20.825831232745166 25 22.494298403552996 27.739767134797745 28.363941903733046 21.401992557916216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 5.0 2 5.5 3 6.0 4 6.0 5 6.0 6 7.5 7 9.0 8 9.0 9 9.0 10 14.5 11 20.0 12 20.0 13 20.0 14 36.5 15 53.0 16 53.0 17 53.0 18 88.0 19 123.0 20 123.0 21 123.0 22 208.5 23 294.0 24 294.0 25 294.0 26 402.5 27 511.0 28 511.0 29 511.0 30 610.5 31 710.0 32 710.0 33 710.0 34 820.0 35 930.0 36 930.0 37 930.0 38 975.0 39 1020.0 40 1020.0 41 1020.0 42 1038.5 43 1057.0 44 1057.0 45 1057.0 46 986.0 47 915.0 48 915.0 49 915.0 50 895.5 51 876.0 52 876.0 53 876.0 54 769.5 55 663.0 56 663.0 57 663.0 58 572.5 59 482.0 60 482.0 61 482.0 62 398.5 63 315.0 64 315.0 65 315.0 66 251.5 67 188.0 68 188.0 69 188.0 70 135.0 71 82.0 72 82.0 73 82.0 74 63.0 75 44.0 76 44.0 77 44.0 78 30.5 79 17.0 80 17.0 81 17.0 82 11.5 83 6.0 84 6.0 85 6.0 86 4.5 87 3.0 88 3.0 89 3.0 90 2.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012003360941063497 2 0.0 3 0.0 4 0.0 5 0.024006721882126995 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 8331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.11919337414476 #Duplication Level Percentage of deduplicated Percentage of total 1 98.62810530218762 95.78682030968672 2 1.0134717587442836 1.9685511943344134 3 0.160672351996045 0.4681310767014764 4 0.03707823507601038 0.14404033129276198 5 0.04943764676801385 0.24006721882126997 6 0.0 0.0 7 0.012359411692003462 0.08402352658744448 8 0.0 0.0 9 0.024718823384006924 0.21606049693914295 >10 0.07415647015202076 1.0923058456367782 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 24 0.28808066258552395 No Hit GGTATCAACGCAGAGTACTTTTTTT 23 0.2760773016444605 No Hit TATCAACGCAGAGTACTTTTTTTTT 13 0.15604369223382547 No Hit GTGGTATCAACGCAGAGTACATGGG 11 0.13203697035169848 No Hit GGTATCAACGCAGAGTACATGGGGG 10 0.12003360941063498 No Hit GAATGGAATGGAATGGAATGGAATG 10 0.12003360941063498 No Hit ATTCCATTCCATTCCATTCCATTCC 9 0.10803024846957147 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9 0.10803024846957147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE