FastQCFastQC Report
Wed 25 May 2016
SRR1295218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA2783.328145576439603No Hit
GCTTATGTACTCTGCGTTGATACCA1471.7598467616425237No Hit
CTTATGTACTCTGCGTTGATACCAC1081.2929486412067521No Hit
GAGTACATAAGCAGTGGTATCAACG820.9816832275829043No Hit
TATGTACTCTGCGTTGATACCACTG590.7063330539925775No Hit
CCCATGTACTCTGCGTTGATACCAC580.6943613073147372No Hit
GCGTTGATACCACTGCTTATGTACT510.6105590805698552No Hit
ATCATGTACTCTGCGTTGATACCAC480.5746438405363342No Hit
CATGTACTCTGCGTTGATACCACTG470.5626720938584939No Hit
CATAAGCAGTGGTATCAACGCAGAG440.5267568538249731No Hit
GTACATGATAAGCAGTGGTATCAAC430.5147851071471328No Hit
GTATCAACGCAGAGTACATAAGCAG390.46689812043577156No Hit
ACGCAGAGTACATAAGCAGTGGTAT370.442954627080091No Hit
GGTATCAACGCAGAGTACATAAGCA350.4190111337244104No Hit
ACTCTGCGTTGATACCACTGCTTAT320.3830958936908895No Hit
CTGCTTATGTACTCTGCGTTGATAC310.3711241470130492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA260.31126541362384774No Hit
GCAGTGGTATCAACGCAGAGTACAT250.2992936669460074No Hit
AAGCAGTGGTATCAACGCAGAGTAC230.2753501735903268No Hit
TATCAACGCAGAGTACATAAGCAGT210.25140668023464624No Hit
GCTTATCCCATGTACTCTGCGTTGA210.25140668023464624No Hit
GTACTCTGCGTTGATACCACTGCTT180.21549144020112532No Hit
GTGGTATCAACGCAGAGTACATAAG170.20351969352328503No Hit
ACATAAGCAGTGGTATCAACGCAGA170.20351969352328503No Hit
CAGTGGTATCAACGCAGAGTACATA160.19154794684544474No Hit
GTACATGGGAAGCAGTGGTATCAAC160.19154794684544474No Hit
GTTGATACCACTGCTTATGTACTCT160.19154794684544474No Hit
ACGCAGAGTACATGATAAGCAGTGG150.17957620016760445No Hit
GATACCACTGCTTATGTACTCTGCG150.17957620016760445No Hit
GAGTACATGATAAGCAGTGGTATCA140.16760445348976416No Hit
GAGTACATGGGATAAGCAGTGGTAT130.15563270681192387No Hit
CCACTGCTTATGTACTCTGCGTTGA120.14366096013408355No Hit
GATAAGCAGTGGTATCAACGCAGAG120.14366096013408355No Hit
GTATCAACGCAGAGTACTTTTTTTT120.14366096013408355No Hit
GCGTTGATACCACTGCTTATCCCAT110.13168921345624326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN110.13168921345624326No Hit
CCCCATGTACTCTGCGTTGATACCA110.13168921345624326No Hit
GTACATGGGATAAGCAGTGGTATCA110.13168921345624326No Hit
GTGGTATCAACGCAGAGTACATGGG110.13168921345624326No Hit
TACCACTGCTTATGTACTCTGCGTT100.11971746677840298No Hit
TGATACCACTGCTTATGTACTCTGC100.11971746677840298No Hit
GTACTTTTTTTTTTTTTTTTTTTTT100.11971746677840298No Hit
GCGTTGATACCACTGCTTATCATGT100.11971746677840298No Hit
GTATCAACGCAGAGTACATGATAAG100.11971746677840298No Hit
TATCAACGCAGAGTACATGATAAGC90.10774572010056266No Hit
GCAGAGTACATAAGCAGTGGTATCA90.10774572010056266No Hit
GCTTATCATGTACTCTGCGTTGATA90.10774572010056266No Hit
GAGTACATGGGAAGCAGTGGTATCA90.10774572010056266No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)