FastQCFastQC Report
Wed 25 May 2016
SRR1295218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA2763.304202083083922No Hit
GCTTATGTACTCTGCGTTGATACCA1862.226744882078295No Hit
CTTATGTACTCTGCGTTGATACCAC1171.4006943613073146No Hit
GAGTACATAAGCAGTGGTATCAACG730.8739375074823417No Hit
CCCATGTACTCTGCGTTGATACCAC620.7422482940260984No Hit
ACTCTGCGTTGATACCACTGCTTAT590.7063330539925775No Hit
TATGTACTCTGCGTTGATACCACTG570.6823895606368969No Hit
GCGTTGATACCACTGCTTATGTACT510.6105590805698552No Hit
ATCATGTACTCTGCGTTGATACCAC480.5746438405363342No Hit
CTGCTTATGTACTCTGCGTTGATAC420.5028133604692925No Hit
CATGTACTCTGCGTTGATACCACTG420.5028133604692925No Hit
TATCAACGCAGAGTACATAAGCAGT420.5028133604692925No Hit
GTATCAACGCAGAGTACATAAGCAG410.49084161379145214No Hit
GTACATGATAAGCAGTGGTATCAAC410.49084161379145214No Hit
CATAAGCAGTGGTATCAACGCAGAG410.49084161379145214No Hit
ACGCAGAGTACATAAGCAGTGGTAT380.4549263737579313No Hit
GTATCAACGCAGAGTACTTTTTTTT370.442954627080091No Hit
AAGCAGTGGTATCAACGCAGAGTAC320.3830958936908895No Hit
GTACATGGGATAAGCAGTGGTATCA290.3471806536573686No Hit
GGTATCAACGCAGAGTACTTTTTTT270.32323716030168803No Hit
GGTATCAACGCAGAGTACATAAGCA250.2992936669460074No Hit
GTACTCTGCGTTGATACCACTGCTT220.26337842691248653No Hit
GTACATGGGAAGCAGTGGTATCAAC200.23943493355680595No Hit
GTACTTTTTTTTTTTTTTTTTTTTT200.23943493355680595No Hit
CCCCATGTACTCTGCGTTGATACCA190.22746318687896566No Hit
TATCAACGCAGAGTACTTTTTTTTT190.22746318687896566No Hit
GCTTATCCCATGTACTCTGCGTTGA180.21549144020112532No Hit
GTGGTATCAACGCAGAGTACATAAG180.21549144020112532No Hit
GAGTACATGATAAGCAGTGGTATCA170.20351969352328503No Hit
GCAGTGGTATCAACGCAGAGTACAT170.20351969352328503No Hit
CTTATACACATCTCCGAGCCCACGA170.20351969352328503No Hit
ATACCACTGCTTATGTACTCTGCGT170.20351969352328503No Hit
ACATAAGCAGTGGTATCAACGCAGA160.19154794684544474No Hit
GCGTTGATACCACTGCTTATCATGT160.19154794684544474No Hit
GGATAAGCAGTGGTATCAACGCAGA150.17957620016760445No Hit
ACGCAGAGTACATGATAAGCAGTGG140.16760445348976416No Hit
ACGCAGAGTACTTTTTTTTTTTTTT140.16760445348976416No Hit
CCACTGCTTATGTACTCTGCGTTGA130.15563270681192387No Hit
CAGTGGTATCAACGCAGAGTACATA130.15563270681192387No Hit
GATAAGCAGTGGTATCAACGCAGAG130.15563270681192387No Hit
GAGTACTTTTTTTTTTTTTTTTTTT130.15563270681192387No Hit
GATACCACTGCTTATGTACTCTGCG130.15563270681192387No Hit
GTGGTATCAACGCAGAGTACATGGG130.15563270681192387No Hit
GGTATCAACGCAGAGTACATGGGAT120.14366096013408355No Hit
CTGCTTATCATGTACTCTGCGTTGA120.14366096013408355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA110.13168921345624326No Hit
ATCAACGCAGAGTACATAAGCAGTG110.13168921345624326No Hit
ATGATAAGCAGTGGTATCAACGCAG110.13168921345624326No Hit
GTTGATACCACTGCTTATGTACTCT110.13168921345624326No Hit
CAGTGGTATCAACGCAGAGTACATG100.11971746677840298No Hit
TACCACTGCTTATGTACTCTGCGTT100.11971746677840298No Hit
GCTTATCATGTACTCTGCGTTGATA100.11971746677840298No Hit
GTATCAACGCAGAGTACATGATAAG90.10774572010056266No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)