FastQCFastQC Report
Wed 25 May 2016
SRR1295217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT250.34274746366876885No Hit
GGTATCAACGCAGAGTACTTTTTTT230.31532766657526734No Hit
TATCAACGCAGAGTACTTTTTTTTT120.16451878256100905No Hit
GGTATCAACGCAGAGTACATGGGGA90.12338908692075677No Hit
GTGGTATCAACGCAGAGTACATGGG80.10967918837400603No Hit
GAATGGAATGGAATGGAATGGAATG80.10967918837400603No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)