##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295217_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7294 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.448039484507813 31.0 31.0 33.0 28.0 34.0 2 31.48629010145325 31.0 31.0 34.0 28.0 34.0 3 31.68110775980258 31.0 31.0 34.0 28.0 34.0 4 35.300658075130244 37.0 35.0 37.0 33.0 37.0 5 35.44036194132163 37.0 35.0 37.0 33.0 37.0 6 35.32561009048533 37.0 35.0 37.0 33.0 37.0 7 35.29503701672608 37.0 35.0 37.0 33.0 37.0 8 35.226487523992326 37.0 35.0 37.0 32.0 37.0 9 36.99067726898821 39.0 37.0 39.0 33.0 39.0 10 36.961886482040036 39.0 37.0 39.0 33.0 39.0 11 37.02399232245681 39.0 37.0 39.0 33.0 39.0 12 36.96257197696737 39.0 37.0 39.0 33.0 39.0 13 36.911708253358924 39.0 37.0 39.0 33.0 39.0 14 38.18275294762819 40.0 38.0 41.0 33.0 41.0 15 38.160268714011515 40.0 38.0 41.0 33.0 41.0 16 38.115300246778176 40.0 38.0 41.0 33.0 41.0 17 38.10913079243213 40.0 38.0 41.0 33.0 41.0 18 38.129695640252265 40.0 38.0 41.0 33.0 41.0 19 38.217027693995064 40.0 38.0 41.0 33.0 41.0 20 38.19344666849465 40.0 38.0 41.0 33.0 41.0 21 38.19673704414587 40.0 38.0 41.0 33.0 41.0 22 38.14916369618865 40.0 38.0 41.0 33.0 41.0 23 38.12736495749932 40.0 38.0 41.0 33.0 41.0 24 38.16822045516863 40.0 38.0 41.0 33.0 41.0 25 36.993282149712094 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.6581678462630798 1101 2 0.4594633761300422 1101 3 -1.1631526322002443 1101 4 0.17028280361614634 1101 5 0.47670609813466314 1101 6 0.10225620939907287 1101 7 0.8693187764616255 1101 8 0.6008352317876202 1101 9 -1.910441322346074 1101 10 0.31488749345893297 1101 11 -1.6967062919443947 1101 12 -0.606232656232649 1101 13 0.7172463339129962 1101 14 0.345126037983178 1101 15 -1.22499290832625 1101 16 -0.6195363719173201 1101 17 0.42353563544039474 1101 18 1.1196915958322506E-4 1101 19 0.4053049243525422 1101 20 0.2353599663123518 1101 21 0.4998690022499517 1101 22 0.7894304108589765 1101 23 0.9032440222916378 1101 24 0.3876415471653374 1101 25 -1.9941200774534096 1102 1 -1.2692789573741905 1102 2 -0.37386995720328997 1102 3 -0.052041521089133624 1102 4 -0.4686060852727465 1102 5 0.3378172092457774 1102 6 0.4911450982879657 1102 7 0.8693187764616255 1102 8 1.1008352317876202 1102 9 0.11733645543170468 1102 10 1.314887493458933 1102 11 1.2477381525000482 1102 12 1.3382117882117939 1102 13 0.35613522280188903 1102 14 1.4006815935387351 1102 15 1.9138959805626357 1102 16 1.0193525169715727 1102 17 -0.49313103122626956 1102 18 1.2501119691595832 1102 19 1.599749368796985 1102 20 -0.8757511447987554 1102 21 0.6387578911388374 1102 22 1.261652633081198 1102 23 1.4032440222916378 1102 24 -0.7790251195013269 1102 25 -0.9385645218978524 1103 1 0.487131299036065 1103 2 0.07484799151465893 1103 3 0.4351379660903518 1103 4 0.1531887865221293 1103 5 -1.2583366369080693 1103 6 -0.11141900427613649 1103 7 -0.2845273773845278 1103 8 -1.1427545118021243 1103 9 -2.7224071343118794 1103 10 0.08411826268969946 1103 11 0.7733791781410773 1103 12 -1.5207625707625638 1103 13 -1.4964288797622132 1103 14 0.2083739012310417 1103 15 0.07415239081905156 1103 16 -0.4485962009771498 1103 17 0.5260997380044969 1103 18 1.0770350460826634 1103 19 -0.38101986197224846 1103 20 -0.20908447813209108 1103 21 -0.2779087755278269 1103 22 -0.2960396746111087 1103 23 0.518628637676251 1103 24 0.2594364189602132 1103 25 1.843486760153425 1104 1 -0.43594562404085835 1104 2 0.0594633761300436 1104 3 0.5812918122441957 1104 4 -0.4852727519394122 1104 5 0.0878172092457774 1104 6 -0.6421882350453672 1104 7 -0.5306812235383731 1104 8 0.9341685651209559 1104 9 -0.1993302112349582 1104 10 0.11488749345893012 1104 11 0.08107148583338386 1104 12 -1.8284548784548704 1104 13 -1.0271981105314438 1104 14 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-1.0967559777083622 2205 24 0.5391566986804932 2205 25 0.5917385084051787 2206 1 0.1095089214136884 2206 2 0.5503724670391357 2206 3 0.372200903153292 2206 4 0.16018179351513595 2206 5 0.6787263001548709 2206 6 0.65781176495463 2206 7 0.8693187764616255 2206 8 -1.7021950712426772 2206 9 -0.5266029385076862 2206 10 -0.1396579610865203 2206 11 -0.4643830596211629 2206 12 -0.46481851481850356 2206 13 -0.9090162923496266 2206 14 0.35522704808418837 2206 15 -0.9118615951949351 2206 16 -0.24579899817994288 2206 17 -1.515858303953543 2206 18 -0.45443348538587003 2206 19 -0.8775233584757416 2206 20 -0.9363572054048177 2206 21 -2.0051815028005535 2206 22 -0.8065291851006222 2206 23 -2.0058468867992687 2206 24 -2.5517523922286003 2206 25 0.7735566902233586 2207 1 0.06405437595914165 2207 2 0.2927967094633743 2207 3 0.4479584789108628 2207 4 0.1147272480605892 2207 5 0.6711505425791131 2207 6 0.4911450982879657 2207 7 1.0359854431282898 2207 8 1.0175018984542916 2207 9 1.4506697887650404 2207 10 0.39822082679226867 2207 11 1.2477381525000482 2207 12 0.9215451215451296 2207 13 1.4394685561352176 2207 14 -0.09931840646126489 2207 15 1.7472293138959714 2207 16 1.436019183638237 2207 17 1.2568689687737304 2207 18 2.0001119691595832 2207 19 -1.900250631203015 2207 20 0.8742488552012446 2207 21 0.3887578911388374 2207 22 -1.6550140335854664 2207 23 -0.5134226443750265 2207 24 1.0543082138320088 2207 25 -0.35523118856452385 2208 1 -0.13594562404085764 2208 2 -0.14053662386995924 2208 3 -0.41870818775580076 2208 4 0.21472724806059063 2208 5 0.5878172092457774 2208 6 0.5578117649546286 2208 7 0.6693187764616226 2208 8 0.13416856512095876 2208 9 0.5006697887650375 2208 10 0.6148874934589301 2208 11 -0.2189285141666133 2208 12 1.1715451215451296 2208 13 0.5728018894685505 2208 14 0.10068159353873796 2208 15 0.2972293138959685 2208 16 -0.16398081636176443 2208 17 0.9568689687737333 2208 18 0.5001119691595832 2208 19 0.849749368796985 2208 20 0.9909155218679118 2208 21 1.422091224472176 2208 22 0.8116526330811951 2208 23 1.4032440222916378 2208 24 1.720974880498673 2208 25 0.4281021447688147 2209 1 -0.15023133832657365 2209 2 0.17374909041575748 2209 3 1.0670060979584832 2209 4 0.5432986766320198 2209 5 -0.3407542193256532 2209 6 0.8006689078117759 2209 7 0.7264616336044796 2209 8 0.5055971365495253 2209 9 -0.15647306837781372 2209 10 1.172030350601787 2209 11 0.009642914404814462 2209 12 0.6715451215451296 2209 13 0.2728018894685533 2209 14 0.6149673078244504 2209 15 0.8543721710388255 2209 16 0.6503048979239523 2209 17 1.3997261116308763 2209 18 0.8572548263024373 2209 19 0.9926065116541309 2209 20 1.2194869504393395 2209 21 1.5792340816150272 2209 22 1.511652633081198 2209 23 -0.5967559777083622 2209 24 0.1495463090701037 2209 25 -1.3433264266597575 2210 1 -0.5192789573741905 2210 2 0.6261300427967065 2210 3 -0.13537485442246933 2210 4 -0.9686060852727465 2210 5 0.1711505425791131 2210 6 0.65781176495463 2210 7 -0.13068122353837452 2210 8 0.6841685651209559 2210 9 0.20066978876504038 2210 10 0.814887493458933 2210 11 0.7477381525000482 2210 12 1.3382117882117939 2210 13 -0.5605314438647824 2210 14 -1.0993184064612649 2210 15 0.7472293138959714 2210 16 1.3526858503049013 2210 17 1.0068689687737304 2210 18 0.7501119691595832 2210 19 0.9330827021303207 2210 20 1.3742488552012446 2210 21 0.2220912244721731 2210 22 2.0949859664145336 2210 23 1.6532440222916378 2210 24 1.8043082138320088 2210 25 -0.6885645218978524 2211 1 -0.5897917778870116 2211 2 0.38254029920696553 2211 3 -1.5648620339096482 2211 4 -0.2314265980932575 2211 5 0.2801249015534708 2211 6 -0.11141900427613649 2211 7 0.638549545692392 2211 8 1.1649377958901894 2211 9 1.046823634918887 2211 10 0.007195185766626366 2211 11 0.9272253319872306 2211 12 0.24846819846820978 2211 13 1.1189557356224 2211 14 -0.1762414833843451 2211 15 0.7664600831267379 2211 16 0.3206345682536238 2211 17 0.29533050723527055 2211 18 0.923188892236503 2211 19 0.6189801380277515 2211 20 -0.5936998627474779 2211 21 1.10670660908756 2211 22 1.7039603253888913 2211 23 1.057090176137791 2211 24 0.6440518035755929 2211 25 1.6127175293841987 2212 1 0.1473877092924738 2212 2 -0.7072032905366257 2212 3 0.6146251455775342 2212 4 -1.7186060852727465 2212 5 0.1711505425791131 2212 6 0.2411450982879657 2212 7 0.9526521097949612 2212 8 0.5175018984542916 2212 9 0.11733645543170468 2212 10 0.48155416012559726 2212 11 -0.16892851416661614 2212 12 0.9215451215451296 2212 13 0.606135222801889 2212 14 -0.3493184064612649 2212 15 0.24722931389597136 2212 16 -0.8973141496950987 2212 17 0.8402023021070661 2212 18 1.083445302492919 2212 19 0.9330827021303207 2212 20 0.7075821885345732 2212 21 0.5554245578055088 2212 22 0.2616526330811979 2212 23 1.4032440222916378 2212 24 0.05430821383200879 2212 25 -0.9385645218978524 2213 1 1.5640543759591417 2213 2 1.2927967094633779 2213 3 1.3646251455775342 2213 4 1.1147272480605892 2213 5 1.0878172092457774 2213 6 1.3244784316212943 2213 7 1.1193187764616255 2213 8 1.1008352317876202 2213 9 1.8673364554317047 2213 10 0.8982208267922687 2213 11 1.7477381525000482 2213 12 1.0048784548784653 2213 13 1.9394685561352176 2213 14 2.484014926872071 2213 15 2.330562647229307 2213 16 1.936019183638237 2213 17 2.340202302107066 2213 18 2.5001119691595832 2213 19 1.599749368796985 2213 20 2.207582188534573 2213 21 1.8054245578055088 2213 22 2.178319299747862 2213 23 1.7365773556249735 2213 24 2.304308213832009 2213 25 2.478102144768812 2214 1 0.980721042625806 2214 2 1.4594633761300422 2214 3 1.2812918122441985 2214 4 1.2813939147272535 2214 5 0.9211505425791131 2214 6 1.3244784316212943 2214 7 0.8693187764616255 2214 8 1.6008352317876202 2214 9 1.6173364554317047 2214 10 1.064887493458933 2214 11 0.33107148583338386 2214 12 1.6715451215451296 2214 13 1.106135222801889 2214 14 0.9006815935387351 2214 15 0.5805626472293071 2214 16 0.5193525169715727 2214 17 1.8402023021070661 2214 18 1.0001119691595832 2214 19 1.849749368796985 2214 20 1.790915521867909 2214 21 1.3887578911388374 2214 22 1.5949859664145336 2214 23 1.7365773556249735 2214 24 1.470974880498673 2214 25 2.728102144768812 2215 1 0.6409774528822219 2215 2 -0.07899816233149792 2215 3 0.6659071968595853 2215 4 0.8454964788298227 2215 5 0.9724325938611642 2215 6 0.8885809957238635 2215 7 0.023164930307778775 2215 8 0.24186087281326252 2215 9 0.8160544041496536 2215 10 0.5456567242281665 2215 11 0.31184071660261736 2215 12 1.2484681984682098 2215 13 -1.5733519566852934 2215 14 -1.2531645603074182 2215 15 -0.7720014553347951 2215 16 0.012942260561317198 2215 17 0.987638199542964 2215 18 0.23088119992881673 2215 19 -0.22717370812609516 2215 20 0.17553090648329572 2215 21 0.9528604552414066 2215 22 1.4731910946196578 2215 23 0.6724747915224043 2215 24 1.0286671881909797 2215 25 2.381948298614965 2216 1 0.008498820403584517 2216 2 0.5705744872411564 2216 3 0.2812918122441985 2216 4 1.1702828036161463 2216 5 0.47670609813466314 2216 6 0.9911450982879657 2216 7 1.4248743320171826 2216 8 1.7119463428987345 2216 9 1.6451142332094832 2216 10 0.9815541601255973 2216 11 1.636627041388941 2216 12 1.9493228993229081 2216 13 -1.2827536660870038 2216 14 -0.09931840646126489 2216 15 -0.33610401943736434 2216 16 0.7137969614160156 2216 17 1.0902023021070661 2216 18 0.5556675247151404 2216 19 0.2941938132414279 2216 20 0.9020266329790232 2216 21 0.6109801133610588 2216 22 -0.21056958914102353 2216 23 1.2365773556249735 2216 24 1.0543082138320088 2216 25 1.3392132558799261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 3.0 11 0.0 12 2.0 13 7.0 14 8.0 15 8.0 16 7.0 17 12.0 18 20.0 19 18.0 20 13.0 21 22.0 22 21.0 23 22.0 24 35.0 25 32.0 26 34.0 27 45.0 28 48.0 29 54.0 30 71.0 31 112.0 32 142.0 33 215.0 34 325.0 35 465.0 36 755.0 37 1343.0 38 3452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52810529202084 20.510008225939128 12.421168083356184 24.54071839868385 2 16.109130792432136 21.593090211132438 38.23690704688785 24.060871949547575 3 18.634306869600987 25.956396544631836 29.9191005073358 25.49019607843137 4 13.038113517959967 17.13737318343844 37.743350699204825 32.08116259939676 5 13.780337309749074 36.473330590977646 34.471410941999174 15.274921157274099 6 30.9569509185632 37.2772141486153 18.05593638607074 13.709898546750754 7 25.96654784754593 32.51987935289279 22.662462297778994 18.851110501782287 8 24.362489717576093 35.714285714285715 20.9350150808884 18.988209487249794 9 24.38990951466959 16.726076227035918 22.86811077598026 36.01590348231423 10 16.150260488072387 28.21497120921305 33.34247326569783 22.292295037016725 11 32.61584864272004 24.266520427748834 23.80038387715931 19.317247052371815 12 22.048539695598517 26.87508569861511 30.549842314548197 20.526532291238173 13 27.90346907993967 22.857534622240504 26.655697243932536 22.58329905388729 14 20.537428023032632 23.581025500411297 28.077872223745544 27.803674252810527 15 23.26569783383603 30.312585686865916 24.431039210309844 21.99067726898821 16 22.292295037016725 29.28434329585961 26.323005209761448 22.100356457362217 17 20.496298327392378 28.639978064162325 28.009322731011792 22.854400877433505 18 19.64628461749383 29.66822045516863 29.24321360021936 21.442281327118177 19 21.25034274746367 28.119001919385795 28.88675623800384 21.743899095146695 20 22.895530573073756 27.74883465862353 28.023032629558543 21.33260213874417 21 23.347957225116534 27.666575267343024 27.74883465862353 21.236632848916916 22 22.278585138469975 27.406087194954758 28.873046339457087 21.442281327118177 23 21.483411022758432 28.050452426652043 28.475459281601317 21.99067726898821 24 21.894707979160955 27.03591993419249 29.435152179873867 21.63421990677269 25 22.292295037016725 27.74883465862353 28.489169180148068 21.46970112421168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.0 3 1.0 4 1.0 5 1.0 6 4.5 7 8.0 8 8.0 9 8.0 10 13.0 11 18.0 12 18.0 13 18.0 14 30.5 15 43.0 16 43.0 17 43.0 18 80.5 19 118.0 20 118.0 21 118.0 22 190.0 23 262.0 24 262.0 25 262.0 26 349.5 27 437.0 28 437.0 29 437.0 30 518.5 31 600.0 32 600.0 33 600.0 34 691.0 35 782.0 36 782.0 37 782.0 38 807.0 39 832.0 40 832.0 41 832.0 42 818.0 43 804.0 44 804.0 45 804.0 46 804.0 47 804.0 48 804.0 49 804.0 50 752.0 51 700.0 52 700.0 53 700.0 54 678.0 55 656.0 56 656.0 57 656.0 58 561.5 59 467.0 60 467.0 61 467.0 62 394.5 63 322.0 64 322.0 65 322.0 66 255.0 67 188.0 68 188.0 69 188.0 70 154.0 71 120.0 72 120.0 73 120.0 74 95.0 75 70.0 76 70.0 77 70.0 78 55.0 79 40.0 80 40.0 81 40.0 82 27.5 83 15.0 84 15.0 85 15.0 86 10.0 87 5.0 88 5.0 89 5.0 90 3.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.013709898546750754 4 0.0 5 0.013709898546750754 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.013709898546750754 13 0.013709898546750754 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7294.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.1894707979161 #Duplication Level Percentage of deduplicated Percentage of total 1 98.58936380307519 95.81848094324103 2 1.072083509662858 2.0839045791061146 3 0.15516998166172943 0.4524266520427749 4 0.04231908590774439 0.16451878256100905 5 0.028212723938496262 0.13709898546750754 6 0.014106361969248131 0.08225939128050452 7 0.014106361969248131 0.09596928982725528 8 0.028212723938496262 0.21935837674801206 9 0.014106361969248131 0.12338908692075677 >10 0.04231908590774439 0.8225939128050452 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 25 0.34274746366876885 No Hit GGTATCAACGCAGAGTACTTTTTTT 23 0.31532766657526734 No Hit TATCAACGCAGAGTACTTTTTTTTT 12 0.16451878256100905 No Hit GGTATCAACGCAGAGTACATGGGGA 9 0.12338908692075677 No Hit GTGGTATCAACGCAGAGTACATGGG 8 0.10967918837400603 No Hit GAATGGAATGGAATGGAATGGAATG 8 0.10967918837400603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE