FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR1295216_2.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 2711 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 25 |
| %GC | 46 |
Per base sequence quality
![[FAIL]](data:image/png;base64,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)
Per tile sequence quality
Per sequence quality scores
Per base sequence content
![[WARN]](data:image/png;base64,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)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTACATAAGCAGTGGTATCAACGCA | 66 | 2.434526005164146 | No Hit |
| GCTTATGTACTCTGCGTTGATACCA | 42 | 1.549243821468093 | No Hit |
| TATGTACTCTGCGTTGATACCACTG | 24 | 0.885282183696053 | No Hit |
| CTTATGTACTCTGCGTTGATACCAC | 23 | 0.8483954260420509 | No Hit |
| GAGTACATAAGCAGTGGTATCAACG | 23 | 0.8483954260420509 | No Hit |
| CCCATGTACTCTGCGTTGATACCAC | 18 | 0.6639616377720399 | No Hit |
| AAAAAAAAAAAAAAAAAAAAAAAAA | 16 | 0.5901881224640354 | No Hit |
| GCGTTGATACCACTGCTTATGTACT | 14 | 0.5164146071560309 | No Hit |
| CTGCTTATGTACTCTGCGTTGATAC | 13 | 0.47952784950202876 | No Hit |
| GTGGTATCAACGCAGAGTACATGGG | 12 | 0.4426410918480265 | No Hit |
| ACGCAGAGTACATAAGCAGTGGTAT | 12 | 0.4426410918480265 | No Hit |
| GTACATGATAAGCAGTGGTATCAAC | 11 | 0.4057543341940244 | No Hit |
| ACTCTGCGTTGATACCACTGCTTAT | 11 | 0.4057543341940244 | No Hit |
| CATAAGCAGTGGTATCAACGCAGAG | 11 | 0.4057543341940244 | No Hit |
| CATGTACTCTGCGTTGATACCACTG | 10 | 0.3688675765400221 | No Hit |
| GTATCAACGCAGAGTACTTTTTTTT | 10 | 0.3688675765400221 | No Hit |
| GTATCAACGCAGAGTACATAAGCAG | 9 | 0.33198081888601993 | No Hit |
| GTACTCTGCGTTGATACCACTGCTT | 8 | 0.2950940612320177 | No Hit |
| GTACATGGGAAGCAGTGGTATCAAC | 8 | 0.2950940612320177 | No Hit |
| GGTATCAACGCAGAGTACATAAGCA | 7 | 0.25820730357801547 | No Hit |
| TATCAACGCAGAGTACATAAGCAGT | 7 | 0.25820730357801547 | No Hit |
| GTACTTTTTTTTTTTTTTTTTTTTT | 7 | 0.25820730357801547 | No Hit |
| ACATAAGCAGTGGTATCAACGCAGA | 6 | 0.22132054592401326 | No Hit |
| GTATCAACGCAGAGTACATGGGGGT | 6 | 0.22132054592401326 | No Hit |
| AAGCAGTGGTATCAACGCAGAGTAC | 6 | 0.22132054592401326 | No Hit |
| GTTAATGATAGTGTGTCGAAACACA | 6 | 0.22132054592401326 | No Hit |
| GATAAGCAGTGGTATCAACGCAGAG | 6 | 0.22132054592401326 | No Hit |
| GTGGTATCAACGCAGAGTACATAAG | 6 | 0.22132054592401326 | No Hit |
| GTATTTAGCCTTGGAGGATGGTCCC | 6 | 0.22132054592401326 | No Hit |
| GAGTACATGATAAGCAGTGGTATCA | 5 | 0.18443378827001106 | No Hit |
| GAGTACATGGGATAAGCAGTGGTAT | 5 | 0.18443378827001106 | No Hit |
| ACCCTGTATCGCGCGCCTTTCCAGA | 5 | 0.18443378827001106 | No Hit |
| GTACATGGGATAAGCAGTGGTATCA | 5 | 0.18443378827001106 | No Hit |
| TACCACTGCTTATGTACTCTGCGTT | 5 | 0.18443378827001106 | No Hit |
| GTACTGGTTCACTATCGGTCAGTCA | 5 | 0.18443378827001106 | No Hit |
| TATCAACGCAGAGTACTTTTTTTTT | 5 | 0.18443378827001106 | No Hit |
| ATCATGTACTCTGCGTTGATACCAC | 5 | 0.18443378827001106 | No Hit |
| GCTTATCATGTACTCTGCGTTGATA | 5 | 0.18443378827001106 | No Hit |
| GGTATCAACGCAGAGTACTTTTTTT | 5 | 0.18443378827001106 | No Hit |
| ATACCACTGCTTATGTACTCTGCGT | 4 | 0.14754703061600885 | No Hit |
| GTACTTAGATGTTTCAGTTCCCCCG | 4 | 0.14754703061600885 | No Hit |
| GTACATGGGGGTTAAGCGACTAAGC | 4 | 0.14754703061600885 | No Hit |
| ATACAGGGTGACAGCCCCGTACACA | 4 | 0.14754703061600885 | No Hit |
| ACTTAGATGTTTCAGTTCCCCCGGT | 4 | 0.14754703061600885 | No Hit |
| GTTCACTATCGGTCAGTCAGGAGTA | 4 | 0.14754703061600885 | No Hit |
| GGATACCACGTGTCCCGCCCTACTC | 4 | 0.14754703061600885 | No Hit |
| CATCTAAGTACCCCGAGGAAAAGAA | 4 | 0.14754703061600885 | No Hit |
| GGGTTTCCCCATTCGGAAATCGCCG | 4 | 0.14754703061600885 | No Hit |
| GGTATCAACGCAGAGTACATGGGAA | 4 | 0.14754703061600885 | No Hit |
| CGGTTGAGCCGTGGGCTTTCACATC | 4 | 0.14754703061600885 | No Hit |
| TCGGTAAGGTGATATGAACCGTTAT | 4 | 0.14754703061600885 | No Hit |
| GATATGAACCGTTATAACCGGCGAT | 4 | 0.14754703061600885 | No Hit |
| ATCATTAACTGAATCCATAGGTTAA | 4 | 0.14754703061600885 | No Hit |
| ACGCAGAGTACTTTTTTTTTTTTTT | 4 | 0.14754703061600885 | No Hit |
| CACTGATTCAGGCTCTGGGCTGCTC | 3 | 0.11066027296200663 | No Hit |
| TATCAACGCAGAGTACATGATAAGC | 3 | 0.11066027296200663 | No Hit |
| GCGTTGATACCACTGCTTATCATGT | 3 | 0.11066027296200663 | No Hit |
| GTGTGTGTGTTAGTGGAAGCGTCTG | 3 | 0.11066027296200663 | No Hit |
| CTATCGGTCAGTCAGGAGTATTTAG | 3 | 0.11066027296200663 | No Hit |
| ATACCACTGCTTCCCATGTACTCTG | 3 | 0.11066027296200663 | No Hit |
| CCACTGCTTATGTACTCTGCGTTGA | 3 | 0.11066027296200663 | No Hit |
| CCCCAGTAGCGGCGAGCGAACGGGG | 3 | 0.11066027296200663 | No Hit |
| CAGTGGTATCAACGCAGAGTACATA | 3 | 0.11066027296200663 | No Hit |
| ATCAACGCAGAGTACATAAGCAGTG | 3 | 0.11066027296200663 | No Hit |
| GCAGTGGTATCAACGCAGAGTACAT | 3 | 0.11066027296200663 | No Hit |
| GTTCATATCACCTTACCGACGCTTA | 3 | 0.11066027296200663 | No Hit |
| GTGTTCAATGACTTCTTCCCTACCC | 3 | 0.11066027296200663 | No Hit |
| GGGTACTTAGATGTTTCAGTTCCCC | 3 | 0.11066027296200663 | No Hit |
| GGAGTATTTAGCCTTGGAGGATGGT | 3 | 0.11066027296200663 | No Hit |
| GGTATCAACGCAGAGTACATGGGGG | 3 | 0.11066027296200663 | No Hit |
| GGTTAATGAGGCGAACCGGGGGAAC | 3 | 0.11066027296200663 | No Hit |
| AGCGTACACGGTGGATGCCCTGGCA | 3 | 0.11066027296200663 | No Hit |
| GCTTATCCCATGTACTCTGCGTTGA | 3 | 0.11066027296200663 | No Hit |
| GTATCAACGCAGAGTACATGGGTGC | 3 | 0.11066027296200663 | No Hit |
| GTGGATGCCCTGGCAGTCAGAGGCG | 3 | 0.11066027296200663 | No Hit |
Adapter Content
Kmer Content
No overrepresented Kmers