##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2711 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.747325710070086 31.0 30.0 33.0 25.0 34.0 2 29.704537071191442 31.0 30.0 33.0 25.0 34.0 3 29.816672814459608 31.0 30.0 33.0 25.0 34.0 4 33.02065658428624 35.0 33.0 37.0 25.0 37.0 5 33.416451493913684 35.0 33.0 37.0 27.0 37.0 6 33.79195868683143 36.0 35.0 37.0 28.0 37.0 7 33.91220951678348 37.0 35.0 37.0 28.0 37.0 8 33.9465142014017 37.0 35.0 37.0 28.0 37.0 9 34.86130579122095 39.0 35.0 39.0 26.0 39.0 10 34.89561047583917 39.0 35.0 39.0 27.0 39.0 11 35.322759129472516 39.0 35.0 39.0 27.0 39.0 12 35.44854297307267 39.0 35.0 39.0 28.0 39.0 13 35.194024345260054 39.0 35.0 39.0 27.0 39.0 14 35.72593139063076 39.0 35.0 41.0 25.0 41.0 15 36.063445223164884 39.0 36.0 41.0 27.0 41.0 16 36.11066027296201 39.0 36.0 41.0 27.0 41.0 17 35.82183696053117 39.0 35.0 41.0 26.0 41.0 18 35.65769088897086 39.0 35.0 41.0 25.0 41.0 19 36.06049428255256 39.0 35.0 41.0 27.0 41.0 20 36.06676503135375 39.0 35.0 41.0 27.0 41.0 21 36.142382884544446 39.0 36.0 41.0 27.0 41.0 22 36.17558096643305 39.0 35.0 41.0 27.0 41.0 23 35.968277388417555 39.0 35.0 41.0 26.0 41.0 24 36.16931021763187 39.0 36.0 41.0 26.0 41.0 25 34.93876798229436 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41496598639455584 1101 2 1.388926681783829 1101 3 1.2800831443688665 1101 4 0.5170445956160208 1101 5 1.8860922146636483 1101 6 2.3417233560090693 1101 7 3.4116780045351476 1101 8 3.070256991685568 1101 9 3.217498110355251 1101 10 3.036167800453512 1101 11 2.636961451247167 1101 12 2.6544595616024225 1101 13 2.912131519274368 1101 14 3.2462585034013642 1101 15 2.577210884353754 1101 16 4.433938019652302 1101 17 3.678495842781558 1101 18 2.6695389266817955 1101 19 3.287679516250954 1101 20 4.470559334845049 1101 21 4.08439153439155 1101 22 3.5304232804232925 1101 23 3.890022675736965 1101 24 3.74637188208618 1101 25 2.671995464852607 1102 1 0.21496598639455655 1102 2 -3.1610733182161717 1102 3 -7.319916855631135 1102 4 -0.9329554043839785 1102 5 -1.6639077853363524 1102 6 -3.4082766439909307 1102 7 -0.5883219954648524 1102 8 0.8702569916855651 1102 9 -4.1825018896447475 1102 10 -3.3638321995464864 1102 11 0.036961451247165655 1102 12 -1.745540438397576 1102 13 -1.087868480725632 1102 14 -2.603741496598637 1102 15 -5.522789115646248 1102 16 -6.266061980347697 1102 17 -6.1215041572184425 1102 18 -3.5804610733182045 1102 19 -2.662320483749049 1102 20 -1.579440665154948 1102 21 1.7343915343915484 1102 22 -1.569576719576709 1102 23 -0.10997732426303486 1102 24 -0.8536281179138214 1102 25 -1.6280045351473902 1103 1 -4.335034013605444 1103 2 0.888926681783829 1103 3 0.5300831443688665 1103 4 1.5170445956160208 1103 5 -1.1139077853363517 1103 6 2.0917233560090693 1103 7 0.6616780045351476 1103 8 1.070256991685568 1103 9 -3.032501889644749 1103 10 -4.963832199546488 1103 11 -4.363038548752833 1103 12 -2.8455404383975775 1103 13 -1.587868480725632 1103 14 -2.0037414965986358 1103 15 -0.6727891156462462 1103 16 -1.3160619803476976 1103 17 -2.821504157218442 1103 18 -1.3304610733182045 1103 19 2.037679516250954 1103 20 3.720559334845049 1103 21 -3.16560846560845 1103 22 -0.9695767195767075 1103 23 -3.609977324263035 1103 24 -1.50362811791382 1103 25 3.171995464852607 1104 1 -1.5850340136054442 1104 2 1.138926681783829 1104 3 -1.7199168556311335 1104 4 -1.2329554043839792 1104 5 -2.3639077853363517 1104 6 -4.408276643990931 1104 7 1.4116780045351476 1104 8 1.070256991685568 1104 9 1.2174981103552511 1104 10 -3.963832199546488 1104 11 0.6369614512471671 1104 12 2.6544595616024225 1104 13 2.912131519274368 1104 14 4.496258503401364 1104 15 -2.922789115646246 1104 16 1.9339380196523024 1104 17 1.1784958427815582 1104 18 1.4195389266817955 1104 19 3.037679516250954 1104 20 4.720559334845049 1104 21 4.83439153439155 1104 22 4.0304232804232925 1104 23 4.390022675736965 1104 24 3.24637188208618 1104 25 5.171995464852607 1105 1 1.2149659863945566 1105 2 1.638926681783829 1105 3 1.0800831443688672 1105 4 1.2670445956160208 1105 5 -0.8639077853363517 1105 6 2.791723356009072 1105 7 2.6116780045351504 1105 8 2.270256991685571 1105 9 3.8174981103552525 1105 10 3.236167800453515 1105 11 2.83696145124717 1105 12 -1.945540438397579 1105 13 0.7121315192743651 1105 14 2.7962585034013614 1105 15 1.0772108843537538 1105 16 1.9339380196523024 1105 17 2.4784958427815553 1105 18 -4.980461073318203 1105 19 -0.6623204837490491 1105 20 2.220559334845049 1105 21 2.934391534391551 1105 22 1.0304232804232925 1105 23 -0.10997732426303486 1105 24 -1.8536281179138214 1105 25 -2.2280045351473916 1106 1 0.8149659863945544 1106 2 -1.361073318216171 1106 3 -2.519916855631134 1106 4 -2.132955404383978 1106 5 -7.663907785336352 1106 6 -1.2082766439909278 1106 7 -2.188321995464854 1106 8 -0.5297430083144334 1106 9 -3.1825018896447475 1106 10 0.4361678004535108 1106 11 -0.9630385487528343 1106 12 -1.1455404383975747 1106 13 -0.6878684807256334 1106 14 -2.803741496598633 1106 15 0.2772108843537566 1106 16 1.1339380196523052 1106 17 0.2784958427815596 1106 18 -3.980461073318203 1106 19 1.337679516250951 1106 20 2.0205593348450464 1106 21 -3.065608465608449 1106 22 -2.9695767195767075 1106 23 -3.5099773242630334 1106 24 -0.45362811791382285 1106 25 -2.028004535147396 1107 1 -0.9183673469387763 1107 2 0.9722600151171612 1107 3 1.6134164777021986 1107 4 -5.399622071050647 1107 5 -2.5305744520030196 1107 6 -5.408276643990931 1107 7 -3.5883219954648524 1107 8 -0.5964096749810963 1107 9 -10.115835222978081 1107 10 -9.29716553287982 1107 11 0.6369614512471671 1107 12 0.32112622826908677 1107 13 -10.087868480725632 1107 14 -6.337074829931968 1107 15 -7.922789115646246 1107 16 -1.732728647014362 1107 17 -1.9881708238851061 1107 18 0.08620559334845979 1107 19 0.5376795162509538 1107 20 -2.779440665154951 1107 21 -8.998941798941782 1107 22 -0.30291005291004325 1107 23 -7.443310657596367 1107 24 -9.25362811791382 1107 25 -2.828004535147393 1108 1 -0.9850340136054427 1108 2 -1.9610733182161724 1108 3 0.08008314436886721 1108 4 2.6670445956160194 1108 5 -0.2639077853363503 1108 6 -4.408276643990931 1108 7 -1.7883219954648517 1108 8 -0.5297430083144334 1108 9 -2.782501889644749 1108 10 -3.963832199546488 1108 11 -0.16303854875283008 1108 12 -3.1455404383975747 1108 13 -1.6878684807256334 1108 14 -3.4037414965986343 1108 15 -0.12278911564624906 1108 16 -3.2660619803477005 1108 17 -0.12150415721843899 1108 18 0.6195389266817983 1108 19 -2.462320483749046 1108 20 -3.579440665154948 1108 21 -3.66560846560845 1108 22 -3.9695767195767075 1108 23 -1.5099773242630334 1108 24 1.9463718820861828 1108 25 -4.628004535147394 1109 1 1.4149659863945558 1109 2 0.13892668178382905 1109 3 1.7800831443688665 1109 4 1.5170445956160208 1109 5 2.1360922146636483 1109 6 3.0917233560090693 1109 7 2.9116780045351476 1109 8 2.570256991685568 1109 9 2.717498110355251 1109 10 2.786167800453512 1109 11 1.386961451247167 1109 12 3.1544595616024225 1109 13 3.412131519274368 1109 14 4.496258503401364 1109 15 1.8272108843537538 1109 16 3.4339380196523024 1109 17 2.678495842781558 1109 18 -1.0804610733182045 1109 19 0.2876795162509538 1109 20 3.970559334845049 1109 21 4.08439153439155 1109 22 3.0304232804232925 1109 23 2.640022675736965 1109 24 1.74637188208618 1109 25 2.171995464852607 1110 1 0.6649659863945558 1110 2 1.638926681783829 1110 3 1.2800831443688665 1110 4 2.267044595616021 1110 5 1.6360922146636483 1110 6 2.0917233560090693 1110 7 1.6616780045351476 1110 8 1.320256991685568 1110 9 2.717498110355251 1110 10 3.536167800453512 1110 11 0.8869614512471671 1110 12 1.9044595616024225 1110 13 1.662131519274368 1110 14 1.7462585034013642 1110 15 -3.672789115646246 1110 16 0.4339380196523024 1110 17 1.1784958427815582 1110 18 1.6695389266817955 1110 19 -1.7123204837490462 1110 20 2.720559334845049 1110 21 -0.16560846560845022 1110 22 2.5304232804232925 1110 23 1.8900226757369651 1110 24 1.99637188208618 1110 25 0.671995464852607 1111 1 1.4149659863945558 1111 2 1.9722600151171612 1111 3 1.1134164777021986 1111 4 2.933711262282685 1111 5 0.8027588813303126 1111 6 1.425056689342405 1111 7 1.2450113378684833 1111 8 1.7369236583522323 1111 9 1.7174981103552511 1111 10 3.036167800453512 1111 11 2.970294784580503 1111 12 2.6544595616024225 1111 13 3.0787981859410323 1111 14 2.3295918367347 1111 15 1.0772108843537538 1111 16 3.267271352985638 1111 17 4.678495842781558 1111 18 3.4195389266817955 1111 19 3.704346182917618 1111 20 2.553892668178385 1111 21 -0.16560846560845022 1111 22 -0.13624338624337184 1111 23 0.39002267573696514 1111 24 2.24637188208618 1111 25 -3.161337868480725 1112 1 1.6649659863945558 1112 2 0.888926681783829 1112 3 1.7800831443688665 1112 4 -1.2329554043839792 1112 5 -0.3639077853363517 1112 6 -2.9082766439909307 1112 7 -2.8383219954648524 1112 8 0.820256991685568 1112 9 -6.782501889644749 1112 10 -2.213832199546488 1112 11 -3.613038548752833 1112 12 -9.345540438397578 1112 13 -5.837868480725632 1112 14 -5.253741496598636 1112 15 -4.672789115646246 1112 16 -7.066061980347698 1112 17 -0.32150415721844183 1112 18 -6.8304610733182045 1112 19 -5.212320483749046 1112 20 -8.27944066515495 1112 21 -8.91560846560845 1112 22 -5.9695767195767075 1112 23 -4.859977324263035 1112 24 -3.50362811791382 1112 25 -3.578004535147393 1113 1 1.2721088435374135 1113 2 0.35321239606954435 1113 3 -1.1484882842025605 1113 4 0.4099017384731667 1113 5 1.2789493575207942 1113 6 1.1631519274376387 1113 7 -1.8740362811791371 1113 8 0.9273998488284221 1113 9 -0.06821617535903357 1113 10 -1.3924036281179184 1113 11 -0.7916099773242635 1113 12 -0.05982615268329283 1113 13 -0.8021541950113473 1113 14 0.28197278911564894 1113 15 1.3629251700680385 1113 16 1.362509448223733 1113 17 1.2499244142101276 1113 18 1.7052532123960802 1113 19 -2.3194633408919003 1113 20 -0.2080120937263814 1113 21 2.33439153439155 1113 22 1.0304232804232925 1113 23 0.31859410430839574 1113 24 0.1749433106576106 1113 25 -0.11371882086167773 1114 1 3.414965986394556 1114 2 3.888926681783829 1114 3 2.7800831443688665 1114 4 3.767044595616021 1114 5 3.6360922146636483 1114 6 3.5917233560090693 1114 7 3.4116780045351476 1114 8 3.070256991685568 1114 9 2.967498110355251 1114 10 3.036167800453512 1114 11 3.636961451247167 1114 12 2.9044595616024225 1114 13 3.412131519274368 1114 14 4.246258503401364 1114 15 3.077210884353754 1114 16 2.1839380196523024 1114 17 1.6784958427815582 1114 18 1.9195389266817955 1114 19 3.287679516250954 1114 20 4.220559334845049 1114 21 4.83439153439155 1114 22 4.5304232804232925 1114 23 4.640022675736965 1114 24 4.49637188208618 1114 25 2.171995464852607 1115 1 -2.385034013605445 1115 2 -0.9610733182161724 1115 3 -2.519916855631134 1115 4 -1.53295540438398 1115 5 -1.6639077853363524 1115 6 -2.4082766439909307 1115 7 -2.188321995464854 1115 8 -3.1297430083144313 1115 9 -2.3825018896447503 1115 10 -0.5638321995464892 1115 11 0.43696145124716423 1115 12 1.254459561602424 1115 13 1.7121315192743651 1115 14 1.5962585034013657 1115 15 2.677210884353755 1115 16 1.9339380196523024 1115 17 3.0784958427815567 1115 18 0.21953892668179265 1115 19 -2.462320483749046 1115 20 -3.3794406651549522 1115 21 0.7343915343915484 1115 22 0.6304232804232939 1115 23 1.4900226757369666 1115 24 -2.053628117913817 1115 25 1.5719954648526056 1116 1 3.214965986394553 1116 2 4.038926681783828 1116 3 3.4800831443688693 1116 4 4.267044595616021 1116 5 3.7360922146636497 1116 6 3.5917233560090693 1116 7 3.4116780045351476 1116 8 2.870256991685565 1116 9 4.217498110355251 1116 10 4.036167800453512 1116 11 3.2369614512471685 1116 12 3.254459561602424 1116 13 3.5121315192743694 1116 14 3.7962585034013614 1116 15 4.277210884353757 1116 16 3.7339380196522995 1116 17 4.27849584278156 1116 18 3.819538926681794 1116 19 3.537679516250954 1116 20 4.620559334845048 1116 21 5.134391534391547 1116 22 4.83042328042329 1116 23 4.290022675736964 1116 24 4.546371882086177 1116 25 6.171995464852607 1201 1 2.164965986394556 1201 2 3.388926681783829 1201 3 1.7800831443688665 1201 4 2.267044595616021 1201 5 3.1360922146636483 1201 6 2.5917233560090693 1201 7 2.9116780045351476 1201 8 2.570256991685568 1201 9 3.717498110355251 1201 10 2.536167800453512 1201 11 3.136961451247167 1201 12 3.6544595616024225 1201 13 3.412131519274368 1201 14 3.4962585034013642 1201 15 4.077210884353754 1201 16 3.6839380196523024 1201 17 3.928495842781558 1201 18 3.4195389266817955 1201 19 3.037679516250954 1201 20 2.720559334845049 1201 21 3.33439153439155 1201 22 4.0304232804232925 1201 23 4.140022675736965 1201 24 2.99637188208618 1201 25 4.421995464852607 1202 1 -2.9183673469387763 1202 2 -2.694406651549503 1202 3 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1203 16 0.9339380196523024 1203 17 -0.5715041572184418 1203 18 1.4195389266817955 1203 19 1.7876795162509538 1203 20 3.970559334845049 1203 21 3.83439153439155 1203 22 2.7804232804232925 1203 23 -0.8599773242630349 1203 24 2.24637188208618 1203 25 -1.828004535147393 1204 1 1.1649659863945558 1204 2 1.638926681783829 1204 3 2.0300831443688665 1204 4 2.517044595616021 1204 5 3.1360922146636483 1204 6 2.0917233560090693 1204 7 2.1616780045351476 1204 8 0.570256991685568 1204 9 3.717498110355251 1204 10 2.786167800453512 1204 11 3.136961451247167 1204 12 3.6544595616024225 1204 13 3.912131519274368 1204 14 -0.003741496598635763 1204 15 3.577210884353754 1204 16 2.9339380196523024 1204 17 -3.071504157218442 1204 18 2.6695389266817955 1204 19 3.787679516250954 1204 20 4.970559334845049 1204 21 5.08439153439155 1204 22 3.2804232804232925 1204 23 0.8900226757369651 1204 24 3.99637188208618 1204 25 5.171995464852607 1205 1 -3.335034013605444 1205 2 -3.611073318216171 1205 3 -3.9699168556311335 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2202 16 2.4339380196523024 2202 17 1.9284958427815582 2202 18 0.9195389266817955 2202 19 -0.21232048374904622 2202 20 0.9705593348450492 2202 21 2.58439153439155 2202 22 3.7804232804232925 2202 23 4.390022675736965 2202 24 1.74637188208618 2202 25 -2.078004535147393 2203 1 -3.9183673469387763 2203 2 -0.027739984882838797 2203 3 0.6134164777021986 2203 4 2.267044595616021 2203 5 3.469425547996984 2203 6 2.2583900226757336 2203 7 2.078344671201812 2203 8 1.7369236583522323 2203 9 2.8841647770219154 2203 10 2.7028344671201765 2203 11 2.970294784580503 2203 12 2.3211262282690868 2203 13 3.2454648526077037 2203 14 2.6629251700680285 2203 15 1.4105442176870895 2203 16 -5.066061980347698 2203 17 1.0118291761148939 2203 18 2.4195389266817955 2203 19 1.8710128495842895 2203 20 0.2205593348450492 2203 21 2.6677248677248855 2203 22 2.0304232804232925 2203 23 3.223356009070301 2203 24 1.0797052154195157 2203 25 2.171995464852607 2204 1 0.41496598639455584 2204 2 1.388926681783829 2204 3 1.0300831443688665 2204 4 0.5170445956160208 2204 5 1.8860922146636483 2204 6 2.0917233560090693 2204 7 2.4116780045351476 2204 8 2.570256991685568 2204 9 0.7174981103552511 2204 10 2.536167800453512 2204 11 1.136961451247167 2204 12 2.6544595616024225 2204 13 2.662131519274368 2204 14 3.2462585034013642 2204 15 0.5772108843537538 2204 16 3.1839380196523024 2204 17 3.678495842781558 2204 18 1.6695389266817955 2204 19 -0.21232048374904622 2204 20 2.470559334845049 2204 21 2.83439153439155 2204 22 2.7804232804232925 2204 23 -1.6099773242630349 2204 24 -0.75362811791382 2204 25 2.921995464852607 2205 1 -5.985034013605443 2205 2 -2.7610733182161695 2205 3 -2.919916855631133 2205 4 -1.9329554043839785 2205 5 1.5360922146636469 2205 6 -6.008276643990932 2205 7 0.011678004535149 2205 8 -4.329743008314431 2205 9 -1.1825018896447475 2205 10 -1.3638321995464864 2205 11 -4.963038548752834 2205 12 -3.1455404383975747 2205 13 -3.6878684807256334 2205 14 -3.803741496598633 2205 15 -0.12278911564624906 2205 16 -1.0660619803476976 2205 17 0.878495842781561 2205 18 0.21953892668179265 2205 19 0.1376795162509552 2205 20 1.2205593348450492 2205 21 -1.6656084656084502 2205 22 1.0304232804232925 2205 23 0.6900226757369623 2205 24 -0.6536281179138186 2205 25 -3.2280045351473916 2206 1 -0.5850340136054442 2206 2 -6.694406651549503 2206 3 -1.7199168556311335 2206 4 -5.066288737717311 2206 5 -5.53057445200302 2206 6 -4.741609977324263 2206 7 -0.5883219954648524 2206 8 -3.5964096749811 2206 9 -5.449168556311417 2206 10 -3.963832199546488 2206 11 -0.6963718820861686 2206 12 -3.3455404383975775 2206 13 -4.421201814058964 2206 14 -1.0037414965986358 2206 15 -0.5894557823129105 2206 16 -4.7327286470143655 2206 17 -5.321504157218442 2206 18 -2.91379440665154 2206 19 -4.1289871504157105 2206 20 0.8872260015117135 2206 21 -0.3322751322751145 2206 22 -1.6362433862433718 2206 23 -0.7766439909296992 2206 24 -6.25362811791382 2206 25 -2.4946712018140573 2207 1 -0.5850340136054442 2207 2 -0.36107331821617095 2207 3 1.5300831443688665 2207 4 -2.482955404383979 2207 5 -1.1139077853363517 2207 6 -0.40827664399093067 2207 7 -3.0883219954648524 2207 8 -2.179743008314432 2207 9 -2.032501889644749 2207 10 -1.9638321995464878 2207 11 -4.113038548752833 2207 12 -0.09554043839757753 2207 13 0.412131519274368 2207 14 -2.5037414965986358 2207 15 -1.4227891156462462 2207 16 -3.5660619803476976 2207 17 -2.071504157218442 2207 18 -1.0804610733182045 2207 19 -0.7123204837490462 2207 20 -2.029440665154951 2207 21 -1.6656084656084502 2207 22 0.28042328042329245 2207 23 -3.359977324263035 2207 24 -5.25362811791382 2207 25 0.671995464852607 2208 1 -4.835034013605444 2208 2 -5.111073318216171 2208 3 -5.4699168556311335 2208 4 -6.732955404383979 2208 5 -4.863907785336352 2208 6 -4.658276643990931 2208 7 -9.338321995464852 2208 8 -10.929743008314432 2208 9 -0.03250188964474887 2208 10 -5.713832199546488 2208 11 -4.113038548752833 2208 12 -7.3455404383975775 2208 13 -11.587868480725632 2208 14 -7.753741496598636 2208 15 -7.172789115646246 2208 16 -2.0660619803476976 2208 17 -4.321504157218442 2208 18 -6.0804610733182045 2208 19 -7.212320483749046 2208 20 -9.52944066515495 2208 21 -2.16560846560845 2208 22 -9.469576719576708 2208 23 -3.109977324263035 2208 24 -1.00362811791382 2208 25 -8.828004535147393 2209 1 3.0149659863945573 2209 2 1.638926681783829 2209 3 -1.319916855631135 2209 4 1.6670445956160194 2209 5 2.9360922146636454 2209 6 2.3917233560090665 2209 7 3.4116780045351476 2209 8 2.6702569916855694 2209 9 4.217498110355251 2209 10 3.036167800453512 2209 11 2.636961451247167 2209 12 2.8544595616024253 2209 13 3.5121315192743694 2209 14 3.7962585034013614 2209 15 4.277210884353757 2209 16 4.133938019652305 2209 17 5.078495842781557 2209 18 5.219538926681793 2209 19 4.137679516250955 2209 20 3.6205593348450478 2209 21 2.134391534391547 2209 22 3.2304232804232953 2209 23 3.4900226757369666 2209 24 4.146371882086179 2209 25 4.771995464852608 2210 1 0.6649659863945558 2210 2 0.888926681783829 2210 3 0.2800831443688665 2210 4 1.5170445956160208 2210 5 -1.6139077853363517 2210 6 -3.6582766439909307 2210 7 0.6616780045351476 2210 8 -4.429743008314432 2210 9 -3.532501889644749 2210 10 -3.713832199546488 2210 11 -4.113038548752833 2210 12 -2.8455404383975775 2210 13 -4.087868480725632 2210 14 -1.0037414965986358 2210 15 -5.172789115646246 2210 16 -4.566061980347698 2210 17 -3.821504157218442 2210 18 -8.080461073318205 2210 19 -2.462320483749046 2210 20 -4.029440665154951 2210 21 -1.1656084656084502 2210 22 -0.7195767195767075 2210 23 -2.609977324263035 2210 24 0.99637188208618 2210 25 -3.078004535147393 2211 1 0.5816326530612237 2211 2 -2.027739984882839 2211 3 -0.2199168556311335 2211 4 -0.8996220710506471 2211 5 -0.030574452003016006 2211 6 1.5917233560090693 2211 7 -0.4216553287981881 2211 8 -0.429743008314432 2211 9 -1.7825018896447489 2211 10 -1.9638321995464878 2211 11 0.9702947845805028 2211 12 -2.1788737717309132 2211 13 -4.087868480725632 2211 14 -0.5037414965986358 2211 15 -1.756122448979582 2211 16 1.267271352985638 2211 17 2.3451625094482225 2211 18 -0.9137944066515402 2211 19 -1.9623204837490462 2211 20 -0.6127739984882865 2211 21 -0.4989417989417859 2211 22 -1.3029100529100432 2211 23 -1.2766439909296992 2211 24 -2.4202947845804843 2211 25 1.3386621315192713 2212 1 -0.18503401360544558 2212 2 -0.5610733182161702 2212 3 1.880083144368868 2212 4 -2.53295540438398 2212 5 -1.6639077853363524 2212 6 1.5917233560090693 2212 7 -2.388321995464853 2212 8 -4.329743008314431 2212 9 1.417498110355254 2212 10 -1.1638321995464906 2212 11 -4.963038548752834 2212 12 -4.945540438397579 2212 13 -3.6878684807256334 2212 14 -4.8037414965986365 2212 15 -4.922789115646246 2212 16 -3.0660619803476976 2212 17 1.4784958427815553 2212 18 -2.3804610733182017 2212 19 1.7376795162509566 2212 20 -0.579440665154948 2212 21 -4.265608465608452 2212 22 -1.3695767195767061 2212 23 -0.909977324263032 2212 24 -1.25362811791382 2212 25 -1.0280045351473959 2213 1 2.8149659863945544 2213 2 2.838926681783832 2213 3 1.680083144368865 2213 4 2.867044595616022 2213 5 2.9360922146636454 2213 6 2.5917233560090693 2213 7 3.4116780045351476 2213 8 2.6702569916855694 2213 9 3.0174981103552483 2213 10 2.436167800453511 2213 11 3.0369614512471657 2213 12 3.6544595616024225 2213 13 3.112131519274371 2213 14 -0.40374149659863434 2213 15 2.877210884353751 2213 16 2.533938019652304 2213 17 3.4784958427815553 2213 18 2.819538926681794 2213 19 3.137679516250955 2213 20 3.420559334845052 2213 21 1.3343915343915498 2213 22 3.2304232804232953 2213 23 -0.7099773242630363 2213 24 2.946371882086183 2213 25 4.171995464852607 2214 1 0.5816326530612237 2214 2 2.472260015117161 2214 3 1.2800831443688665 2214 4 2.933711262282685 2214 5 0.8027588813303126 2214 6 1.5917233560090693 2214 7 2.4116780045351476 2214 8 1.570256991685568 2214 9 3.8841647770219154 2214 10 3.536167800453512 2214 11 0.4702947845805028 2214 12 1.9877928949357582 2214 13 -1.2545351473922963 2214 14 0.8295918367346999 2214 15 2.243877551020418 2214 16 -1.0660619803476976 2214 17 1.3451625094482225 2214 18 2.752872260015131 2214 19 2.204346182917618 2214 20 3.053892668178385 2214 21 4.001058201058214 2214 22 3.6970899470899568 2214 23 2.5566893424036294 2214 24 1.74637188208618 2214 25 2.0053287981859427 2215 1 1.4149659863945558 2215 2 -1.361073318216171 2215 3 2.5300831443688665 2215 4 -0.23295540438397921 2215 5 2.3860922146636483 2215 6 2.3417233560090693 2215 7 1.9116780045351476 2215 8 1.820256991685568 2215 9 3.217498110355251 2215 10 2.036167800453512 2215 11 3.136961451247167 2215 12 2.6544595616024225 2215 13 2.412131519274368 2215 14 2.4962585034013642 2215 15 1.3272108843537538 2215 16 3.9339380196523024 2215 17 2.678495842781558 2215 18 3.9195389266817955 2215 19 2.287679516250954 2215 20 -1.0294406651549508 2215 21 0.3343915343915498 2215 22 1.7804232804232925 2215 23 3.890022675736965 2215 24 1.99637188208618 2215 25 3.421995464852607 2216 1 0.6649659863945558 2216 2 0.888926681783829 2216 3 1.5300831443688665 2216 4 2.517044595616021 2216 5 2.8860922146636483 2216 6 1.8417233560090693 2216 7 1.4116780045351476 2216 8 1.070256991685568 2216 9 2.217498110355251 2216 10 1.5361678004535122 2216 11 2.136961451247167 2216 12 0.15445956160242247 2216 13 1.412131519274368 2216 14 2.2462585034013642 2216 15 2.077210884353754 2216 16 1.4339380196523024 2216 17 0.42849584278155817 2216 18 1.4195389266817955 2216 19 0.5376795162509538 2216 20 -0.2794406651549508 2216 21 -6.16560846560845 2216 22 -10.719576719576708 2216 23 -4.359977324263035 2216 24 -6.50362811791382 2216 25 0.921995464852607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 1.0 11 1.0 12 6.0 13 10.0 14 20.0 15 23.0 16 33.0 17 21.0 18 25.0 19 25.0 20 28.0 21 17.0 22 15.0 23 15.0 24 9.0 25 20.0 26 21.0 27 20.0 28 28.0 29 39.0 30 42.0 31 56.0 32 71.0 33 96.0 34 151.0 35 227.0 36 335.0 37 530.0 38 820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.40088593576966 19.453672942045035 12.513842746400886 21.63159837578442 2 15.847801994828222 23.494643516808274 37.56926486885852 23.088289619504987 3 20.98263760620613 26.56076837827854 30.88289619504987 21.57369782046546 4 14.079822616407982 21.06430155210643 30.70953436807095 34.146341463414636 5 12.749445676274945 41.42645971914264 31.263858093126384 14.560236511456024 6 28.370890284447732 34.98337643147396 20.760990025858884 15.88474325821943 7 24.5289988917621 36.017731806427776 20.797931289250094 18.65533801256003 8 23.974879940893977 36.1285555966014 22.12781677133358 17.76874769117104 9 26.2379896526238 21.507760532150776 17.88617886178862 34.368070953436806 10 15.668883961566888 30.266075388026607 29.08351810790835 24.98152254249815 11 30.56171470805617 28.824833702882486 19.955654101995567 20.65779748706578 12 21.877309682187732 28.6770140428677 26.644493717664446 22.801182557280118 13 27.57855822550832 23.65988909426987 27.948243992606287 20.813308687615525 14 24.731977818853974 25.76709796672828 24.879852125693162 24.621072088724585 15 28.133086876155268 28.317929759704253 20.51756007393715 23.031423290203325 16 24.944567627494457 26.792313377679232 26.570583887657058 21.692535107169252 17 21.019578869597343 31.547838936091615 24.233468784632432 23.199113409678613 18 22.505543237250556 25.905395417590537 31.559497413155952 20.029563932002954 19 21.66358595194085 32.82809611829944 25.47134935304991 20.036968576709796 20 22.846580406654343 27.985212569316083 28.022181146025876 21.146025878003698 21 24.242424242424242 31.042128603104214 23.059866962305986 21.65558019216556 22 20.768662232076863 29.82261640798226 22.801182557280118 26.607538802660752 23 24.953789279112755 26.46950092421442 24.54713493530499 24.029574861367838 24 22.292051756007396 29.094269870609978 23.253234750462106 25.36044362292052 25 23.58595194085028 28.946395563770793 24.10351201478743 23.364140480591498 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19.0 1 19.0 2 14.5 3 10.0 4 10.0 5 10.0 6 11.5 7 13.0 8 13.0 9 13.0 10 14.5 11 16.0 12 16.0 13 16.0 14 19.0 15 22.0 16 22.0 17 22.0 18 32.0 19 42.0 20 42.0 21 42.0 22 52.0 23 62.0 24 62.0 25 62.0 26 82.0 27 102.0 28 102.0 29 102.0 30 115.0 31 128.0 32 128.0 33 128.0 34 153.5 35 179.0 36 179.0 37 179.0 38 211.0 39 243.0 40 243.0 41 243.0 42 392.0 43 541.0 44 541.0 45 541.0 46 456.0 47 371.0 48 371.0 49 371.0 50 322.0 51 273.0 52 273.0 53 273.0 54 243.0 55 213.0 56 213.0 57 213.0 58 191.0 59 169.0 60 169.0 61 169.0 62 148.5 63 128.0 64 128.0 65 128.0 66 101.5 67 75.0 68 75.0 69 75.0 70 67.0 71 59.0 72 59.0 73 59.0 74 42.0 75 25.0 76 25.0 77 25.0 78 20.0 79 15.0 80 15.0 81 15.0 82 10.0 83 5.0 84 5.0 85 5.0 86 3.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07377351530800443 2 0.14754703061600885 3 0.14754703061600885 4 0.18443378827001106 5 0.18443378827001106 6 0.14754703061600885 7 0.14754703061600885 8 0.14754703061600885 9 0.18443378827001106 10 0.18443378827001106 11 0.18443378827001106 12 0.18443378827001106 13 0.22132054592401326 14 0.22132054592401326 15 0.22132054592401326 16 0.18443378827001106 17 0.14754703061600885 18 0.18443378827001106 19 0.22132054592401326 20 0.22132054592401326 21 0.18443378827001106 22 0.18443378827001106 23 0.22132054592401326 24 0.22132054592401326 25 0.22132054592401326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2711.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.20398376982664 #Duplication Level Percentage of deduplicated Percentage of total 1 90.8743430482561 70.1586130579122 2 5.542283803153368 8.557727775728512 3 1.0033444816053512 2.3238657322021394 4 0.716674629718108 2.213205459240133 5 0.4777830864787387 1.8443378827001107 6 0.33444816053511706 1.549243821468093 7 0.1433349259436216 0.7746219107340465 8 0.09555661729574773 0.5901881224640354 9 0.047778308647873864 0.33198081888601993 >10 0.716674629718108 9.221689413500552 >50 0.047778308647873864 2.434526005164146 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 66 2.434526005164146 No Hit GCTTATGTACTCTGCGTTGATACCA 42 1.549243821468093 No Hit TATGTACTCTGCGTTGATACCACTG 24 0.885282183696053 No Hit CTTATGTACTCTGCGTTGATACCAC 23 0.8483954260420509 No Hit GAGTACATAAGCAGTGGTATCAACG 23 0.8483954260420509 No Hit CCCATGTACTCTGCGTTGATACCAC 18 0.6639616377720399 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 16 0.5901881224640354 No Hit GCGTTGATACCACTGCTTATGTACT 14 0.5164146071560309 No Hit CTGCTTATGTACTCTGCGTTGATAC 13 0.47952784950202876 No Hit GTGGTATCAACGCAGAGTACATGGG 12 0.4426410918480265 No Hit ACGCAGAGTACATAAGCAGTGGTAT 12 0.4426410918480265 No Hit GTACATGATAAGCAGTGGTATCAAC 11 0.4057543341940244 No Hit ACTCTGCGTTGATACCACTGCTTAT 11 0.4057543341940244 No Hit CATAAGCAGTGGTATCAACGCAGAG 11 0.4057543341940244 No Hit CATGTACTCTGCGTTGATACCACTG 10 0.3688675765400221 No Hit GTATCAACGCAGAGTACTTTTTTTT 10 0.3688675765400221 No Hit GTATCAACGCAGAGTACATAAGCAG 9 0.33198081888601993 No Hit GTACTCTGCGTTGATACCACTGCTT 8 0.2950940612320177 No Hit GTACATGGGAAGCAGTGGTATCAAC 8 0.2950940612320177 No Hit GGTATCAACGCAGAGTACATAAGCA 7 0.25820730357801547 No Hit TATCAACGCAGAGTACATAAGCAGT 7 0.25820730357801547 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7 0.25820730357801547 No Hit ACATAAGCAGTGGTATCAACGCAGA 6 0.22132054592401326 No Hit GTATCAACGCAGAGTACATGGGGGT 6 0.22132054592401326 No Hit AAGCAGTGGTATCAACGCAGAGTAC 6 0.22132054592401326 No Hit GTTAATGATAGTGTGTCGAAACACA 6 0.22132054592401326 No Hit GATAAGCAGTGGTATCAACGCAGAG 6 0.22132054592401326 No Hit GTGGTATCAACGCAGAGTACATAAG 6 0.22132054592401326 No Hit GTATTTAGCCTTGGAGGATGGTCCC 6 0.22132054592401326 No Hit GAGTACATGATAAGCAGTGGTATCA 5 0.18443378827001106 No Hit GAGTACATGGGATAAGCAGTGGTAT 5 0.18443378827001106 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 5 0.18443378827001106 No Hit GTACATGGGATAAGCAGTGGTATCA 5 0.18443378827001106 No Hit TACCACTGCTTATGTACTCTGCGTT 5 0.18443378827001106 No Hit GTACTGGTTCACTATCGGTCAGTCA 5 0.18443378827001106 No Hit TATCAACGCAGAGTACTTTTTTTTT 5 0.18443378827001106 No Hit ATCATGTACTCTGCGTTGATACCAC 5 0.18443378827001106 No Hit GCTTATCATGTACTCTGCGTTGATA 5 0.18443378827001106 No Hit GGTATCAACGCAGAGTACTTTTTTT 5 0.18443378827001106 No Hit ATACCACTGCTTATGTACTCTGCGT 4 0.14754703061600885 No Hit GTACTTAGATGTTTCAGTTCCCCCG 4 0.14754703061600885 No Hit GTACATGGGGGTTAAGCGACTAAGC 4 0.14754703061600885 No Hit ATACAGGGTGACAGCCCCGTACACA 4 0.14754703061600885 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 4 0.14754703061600885 No Hit GTTCACTATCGGTCAGTCAGGAGTA 4 0.14754703061600885 No Hit GGATACCACGTGTCCCGCCCTACTC 4 0.14754703061600885 No Hit CATCTAAGTACCCCGAGGAAAAGAA 4 0.14754703061600885 No Hit GGGTTTCCCCATTCGGAAATCGCCG 4 0.14754703061600885 No Hit GGTATCAACGCAGAGTACATGGGAA 4 0.14754703061600885 No Hit CGGTTGAGCCGTGGGCTTTCACATC 4 0.14754703061600885 No Hit TCGGTAAGGTGATATGAACCGTTAT 4 0.14754703061600885 No Hit GATATGAACCGTTATAACCGGCGAT 4 0.14754703061600885 No Hit ATCATTAACTGAATCCATAGGTTAA 4 0.14754703061600885 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4 0.14754703061600885 No Hit CACTGATTCAGGCTCTGGGCTGCTC 3 0.11066027296200663 No Hit TATCAACGCAGAGTACATGATAAGC 3 0.11066027296200663 No Hit GCGTTGATACCACTGCTTATCATGT 3 0.11066027296200663 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 3 0.11066027296200663 No Hit CTATCGGTCAGTCAGGAGTATTTAG 3 0.11066027296200663 No Hit ATACCACTGCTTCCCATGTACTCTG 3 0.11066027296200663 No Hit CCACTGCTTATGTACTCTGCGTTGA 3 0.11066027296200663 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 3 0.11066027296200663 No Hit CAGTGGTATCAACGCAGAGTACATA 3 0.11066027296200663 No Hit ATCAACGCAGAGTACATAAGCAGTG 3 0.11066027296200663 No Hit GCAGTGGTATCAACGCAGAGTACAT 3 0.11066027296200663 No Hit GTTCATATCACCTTACCGACGCTTA 3 0.11066027296200663 No Hit GTGTTCAATGACTTCTTCCCTACCC 3 0.11066027296200663 No Hit GGGTACTTAGATGTTTCAGTTCCCC 3 0.11066027296200663 No Hit GGAGTATTTAGCCTTGGAGGATGGT 3 0.11066027296200663 No Hit GGTATCAACGCAGAGTACATGGGGG 3 0.11066027296200663 No Hit GGTTAATGAGGCGAACCGGGGGAAC 3 0.11066027296200663 No Hit AGCGTACACGGTGGATGCCCTGGCA 3 0.11066027296200663 No Hit GCTTATCCCATGTACTCTGCGTTGA 3 0.11066027296200663 No Hit GTATCAACGCAGAGTACATGGGTGC 3 0.11066027296200663 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 3 0.11066027296200663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.03688675765400221 0.0 11 0.0 0.0 0.0 0.03688675765400221 0.0 12 0.0 0.0 0.0 0.03688675765400221 0.0 13 0.0 0.0 0.0 0.03688675765400221 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE