##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295216_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2711 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.215418664699374 31.0 31.0 33.0 28.0 34.0 2 31.072666912578384 31.0 31.0 34.0 28.0 34.0 3 31.390261895979343 31.0 31.0 34.0 28.0 34.0 4 35.02471412762818 37.0 35.0 37.0 32.0 37.0 5 35.2589450387311 37.0 35.0 37.0 32.0 37.0 6 35.16746587974917 37.0 35.0 37.0 32.0 37.0 7 34.99963113242346 37.0 35.0 37.0 32.0 37.0 8 35.09369236444117 37.0 35.0 37.0 32.0 37.0 9 36.6986351899668 39.0 37.0 39.0 32.0 39.0 10 36.780892659535226 39.0 37.0 39.0 32.0 39.0 11 36.80007377351531 39.0 37.0 39.0 33.0 39.0 12 36.78531907045371 39.0 37.0 39.0 33.0 39.0 13 36.43858354850609 39.0 37.0 39.0 32.0 39.0 14 37.5953522685356 40.0 37.0 41.0 32.0 41.0 15 37.53301364810033 40.0 37.0 41.0 32.0 41.0 16 37.47178163039469 40.0 37.0 41.0 32.0 41.0 17 37.52600516414607 40.0 37.0 41.0 32.0 41.0 18 37.573589081519735 40.0 37.0 41.0 32.0 41.0 19 37.684987089634824 40.0 38.0 41.0 32.0 41.0 20 37.64551825894504 40.0 38.0 41.0 32.0 41.0 21 37.6119513094799 40.0 37.0 41.0 31.0 41.0 22 37.67170785687938 40.0 38.0 41.0 32.0 41.0 23 37.7152342309111 40.0 38.0 41.0 32.0 41.0 24 37.703430468461825 40.0 38.0 41.0 32.0 41.0 25 36.375876060494285 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.158692365835222 1101 2 1.4044217687074756 1101 3 1.1423280423280495 1101 4 1.19520030234316 1101 5 0.8458427815570673 1101 6 -0.9301209372637942 1101 7 -0.9145880574452008 1101 8 0.26092214663642466 1101 9 1.4009070294784536 1101 10 0.6834089191232096 1101 11 -3.577097505668931 1101 12 0.04297052154194603 1101 13 0.6513227513227449 1101 14 1.873091458805753 1101 15 1.0622826908541256 1101 16 2.2001511715797477 1101 17 1.70075585789872 1101 18 2.094935752078598 1101 19 -0.3405139833711388 1101 20 0.71239606953894 1101 21 2.9105442176870824 1101 22 2.353892668178389 1101 23 2.8301209372637928 1101 24 2.3485260770975174 1101 25 0.36489040060468625 1102 1 -2.1413076341647788 1102 2 -4.295578231292524 1102 3 -3.157671957671951 1102 4 -2.4047996976568413 1102 5 -0.3541572184429356 1102 6 -1.1801209372637942 1102 7 -4.764588057445202 1102 8 -3.2390778533635753 1102 9 -4.499092970521545 1102 10 -7.466591080876789 1102 11 -6.27709750566893 1102 12 -7.007029478458055 1102 13 -5.398677248677256 1102 14 -6.426908541194248 1102 15 -2.637717309145877 1102 16 -4.899848828420254 1102 17 -10.74924414210128 1102 18 -8.0550642479214 1102 19 -5.090513983371139 1102 20 -3.53760393046106 1102 21 -3.989455782312916 1102 22 -1.8461073318216137 1102 23 -2.2698790627362087 1102 24 -2.95147392290248 1102 25 -12.635109599395314 1103 1 0.40869236583522195 1103 2 0.6544217687074756 1103 3 0.8923280423280495 1103 4 -0.054799697656839896 1103 5 0.34584278155706727 1103 6 0.06987906273620581 1103 7 0.3354119425547992 1103 8 0.7609221466364247 1103 9 -0.09909297052154642 1103 10 1.4334089191232096 1103 11 0.17290249433106908 1103 12 -0.457029478458054 1103 13 0.6513227513227449 1103 14 -4.626908541194247 1103 15 -1.9377173091458744 1103 16 0.4501511715797477 1103 17 -0.2992441421012799 1103 18 0.8449357520785981 1103 19 1.4094860166288612 1103 20 -2.28760393046106 1103 21 0.4105442176870824 1103 22 0.6038926681783892 1103 23 1.0801209372637928 1103 24 -0.4014739229024826 1103 25 1.6148904006046862 1104 1 -3.841307634164778 1104 2 -1.5955782312925244 1104 3 -1.3576719576719505 1104 4 -13.80479969765684 1104 5 -11.654157218442933 1104 6 -8.680120937263794 1104 7 -3.6645880574452008 1104 8 -2.2390778533635753 1104 9 -2.5990929705215464 1104 10 -5.06659108087679 1104 11 -2.577097505668931 1104 12 -6.207029478458054 1104 13 -8.098677248677255 1104 14 -3.126908541194247 1104 15 -7.937717309145874 1104 16 -7.799848828420252 1104 17 -11.04924414210128 1104 18 -14.155064247921402 1104 19 -3.590513983371139 1104 20 -11.53760393046106 1104 21 -14.089455782312918 1104 22 -14.14610733182161 1104 23 -13.669879062736207 1104 24 -9.151473922902483 1104 25 -11.635109599395314 1105 1 -0.14130763416477876 1105 2 -2.0955782312925244 1105 3 -0.1576719576719512 1105 4 0.7952003023431615 1105 5 1.0458427815570701 1105 6 1.4198790627362072 1105 7 -2.1645880574452008 1105 8 -0.4390778533635782 1105 9 -0.8990929705215436 1105 10 -1.2665910808767933 1105 11 0.5229024943310705 1105 12 0.5929705215419432 1105 13 -1.3986772486772523 1105 14 1.7730914588057516 1105 15 -3.2377173091458715 1105 16 -0.2998488284202523 1105 17 0.8507558578987187 1105 18 -1.655064247921402 1105 19 -4.090513983371139 1105 20 -3.737603930461063 1105 21 -3.3894557823129148 1105 22 -3.446107331821608 1105 23 -1.6698790627362072 1105 24 1.0485260770975202 1105 25 -3.8351095993953166 1106 1 0.8586923658352248 1106 2 1.504421768707477 1106 3 1.6423280423280495 1106 4 1.7952003023431615 1106 5 1.4458427815570687 1106 6 1.0198790627362087 1106 7 0.8354119425547992 1106 8 0.9609221466364275 1106 9 1.500907029478455 1106 10 -0.8665910808767876 1106 11 0.12290249433107192 1106 12 0.9929705215419489 1106 13 1.8013227513227434 1106 14 1.1730914588057502 1106 15 -1.0377173091458758 1106 16 -0.09984882842024945 1106 17 1.45075585789872 1106 18 1.1449357520785952 1106 19 1.3094860166288598 1106 20 2.662396069538943 1106 21 1.2105442176870795 1106 22 1.3538926681783892 1106 23 0.13012093726378993 1106 24 0.8485260770975174 1106 25 1.1648904006046834 1107 1 1.3253590325018862 1107 2 -1.0955782312925244 1107 3 0.6423280423280495 1107 4 1.5285336356764958 1107 5 1.179176114890403 1107 6 0.4865457294028701 1107 7 1.168745275888135 1107 8 1.0942554799697604 1107 9 0.5675736961451179 1107 10 1.6000755857898739 1107 11 1.2562358276644048 1107 12 1.1263038548752817 1107 13 1.7346560846560806 1107 14 -0.2935752078609113 1107 15 0.8956160241874613 1107 16 -0.2998488284202523 1107 17 1.7840891912320558 1107 18 2.0116024187452624 1107 19 1.9094860166288612 1107 20 2.46239606953894 1107 21 1.4105442176870824 1107 22 2.687226001511725 1107 23 2.6634542705971285 1107 24 1.5151927437641817 1107 25 -0.9684429327286495 1108 1 -0.5413076341647773 1108 2 0.30442176870747417 1108 3 0.0423280423280481 1108 4 -0.0047996976568427385 1108 5 1.0458427815570701 1108 6 0.619879062736203 1108 7 0.6354119425547964 1108 8 -0.23907785336357534 1108 9 0.500907029478455 1108 10 -2.2665910808767933 1108 11 -1.077097505668931 1108 12 -5.007029478458051 1108 13 -3.9986772486772537 1108 14 -5.226908541194248 1108 15 -2.837717309145873 1108 16 -2.699848828420251 1108 17 -4.349244142101277 1108 18 -1.455064247921399 1108 19 -0.29051398337114165 1108 20 -0.7376039304610629 1108 21 0.4105442176870824 1108 22 -1.2461073318216123 1108 23 -9.469879062736208 1108 24 -3.551473922902481 1108 25 -4.035109599395312 1109 1 1.158692365835222 1109 2 2.6544217687074756 1109 3 1.1423280423280495 1109 4 1.19520030234316 1109 5 0.8458427815570673 1109 6 1.3198790627362058 1109 7 1.3354119425547992 1109 8 1.2609221466364247 1109 9 1.4009070294784536 1109 10 0.9334089191232096 1109 11 1.922902494331069 1109 12 1.792970521541946 1109 13 2.401322751322745 1109 14 1.373091458805753 1109 15 2.5622826908541256 1109 16 3.2001511715797477 1109 17 2.70075585789872 1109 18 2.344935752078598 1109 19 1.6594860166288612 1109 20 0.21239606953893997 1109 21 2.6605442176870824 1109 22 2.103892668178389 1109 23 2.0801209372637928 1109 24 2.3485260770975174 1109 25 2.3648904006046862 1110 1 -0.09130763416477805 1110 2 1.4044217687074756 1110 3 -0.3576719576719505 1110 4 0.6952003023431601 1110 5 0.8458427815570673 1110 6 -2.180120937263794 1110 7 -2.1645880574452008 1110 8 -0.23907785336357534 1110 9 -0.5990929705215464 1110 10 -3.3165910808767904 1110 11 0.17290249433106908 1110 12 -0.20702947845805397 1110 13 -2.348677248677255 1110 14 -0.876908541194247 1110 15 -0.18771730914587437 1110 16 0.7001511715797477 1110 17 1.20075585789872 1110 18 0.5949357520785981 1110 19 1.1594860166288612 1110 20 1.96239606953894 1110 21 0.4105442176870824 1110 22 0.35389266817838916 1110 23 0.08012093726379277 1110 24 -1.6514739229024826 1110 25 -1.6351095993953138 1111 1 1.158692365835222 1111 2 0.5710884353741434 1111 3 0.14232804232804952 1111 4 1.8618669690098244 1111 5 1.179176114890403 1111 6 0.8198790627362058 1111 7 1.3354119425547992 1111 8 1.427588813303089 1111 9 0.4009070294784536 1111 10 -0.39992441421012614 1111 11 -1.2437641723355952 1111 12 0.4596371882086103 1111 13 0.5679894179894092 1111 14 0.2064247921390887 1111 15 2.22894935752079 1111 16 0.5334845049130834 1111 17 1.95075585789872 1111 18 1.6782690854119338 1111 19 1.4094860166288612 1111 20 0.7957294028722757 1111 21 2.0772108843537467 1111 22 1.5205593348450535 1111 23 2.6634542705971285 1111 24 1.681859410430853 1111 25 -1.9684429327286495 1112 1 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13 2.201322751322742 1116 14 2.9730914588057544 1116 15 2.962282690854124 1116 16 3.500151171579745 1116 17 3.2507558578987172 1116 18 3.144935752078595 1116 19 2.5094860166288626 1116 20 2.662396069538943 1116 21 3.010544217687084 1116 22 2.7538926681783877 1116 23 2.7301209372637913 1116 24 2.248526077097516 1116 25 1.3648904006046862 1201 1 -0.34130763416477805 1201 2 -0.3455782312925244 1201 3 0.14232804232804952 1201 4 -1.05479969765684 1201 5 -1.4041572184429327 1201 6 0.06987906273620581 1201 7 -0.16458805744520077 1201 8 0.26092214663642466 1201 9 0.6509070294784536 1201 10 0.9334089191232096 1201 11 1.672902494331069 1201 12 1.792970521541946 1201 13 2.151322751322745 1201 14 -1.376908541194247 1201 15 0.31228269085412563 1201 16 1.2001511715797477 1201 17 0.7007558578987201 1201 18 1.594935752078598 1201 19 -1.5905139833711388 1201 20 -0.28760393046106003 1201 21 -0.5894557823129176 1201 22 2.603892668178389 1201 23 -0.6698790627362072 1201 24 1.8485260770975174 1201 25 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2.401322751322745 1203 14 3.373091458805753 1203 15 2.5622826908541256 1203 16 1.4501511715797477 1203 17 1.45075585789872 1203 18 1.344935752078598 1203 19 0.6594860166288612 1203 20 -0.03760393046106003 1203 21 1.1605442176870824 1203 22 1.3538926681783892 1203 23 -1.4198790627362072 1203 24 0.5985260770975174 1203 25 1.3648904006046862 1204 1 -0.09130763416477805 1204 2 -0.09557823129252441 1204 3 0.14232804232804952 1204 4 -0.5547996976568399 1204 5 0.09584278155706727 1204 6 0.8198790627362058 1204 7 1.3354119425547992 1204 8 1.2609221466364247 1204 9 0.9009070294784536 1204 10 1.4334089191232096 1204 11 0.9229024943310691 1204 12 1.042970521541946 1204 13 1.9013227513227449 1204 14 -1.126908541194247 1204 15 1.8122826908541256 1204 16 0.9501511715797477 1204 17 1.20075585789872 1204 18 2.094935752078598 1204 19 1.1594860166288612 1204 20 2.21239606953894 1204 21 1.6605442176870824 1204 22 1.3538926681783892 1204 23 -0.16987906273620723 1204 24 2.3485260770975174 1204 25 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-4.989077853363575 2202 9 -3.5990929705215464 2202 10 -0.06659108087679044 2202 11 -0.8270975056689309 2202 12 -4.207029478458054 2202 13 -3.598677248677255 2202 14 -0.626908541194247 2202 15 -2.9377173091458744 2202 16 -5.049848828420252 2202 17 -3.29924414210128 2202 18 -0.15506424792140194 2202 19 -1.3405139833711388 2202 20 -3.78760393046106 2202 21 -0.08945578231291762 2202 22 -1.6461073318216108 2202 23 -5.169879062736207 2202 24 -3.6514739229024826 2202 25 -2.1351095993953138 2203 1 -0.6746409674981102 2203 2 -4.428911564625857 2203 3 -2.3576719576719505 2203 4 -0.4714663643235042 2203 5 0.5125094482237316 2203 6 0.4865457294028701 2203 7 0.5020786092214635 2203 8 0.7609221466364247 2203 9 -1.0990929705215464 2203 10 0.6000755857898739 2203 11 1.5895691609977334 2203 12 1.792970521541946 2203 13 -0.9320105820105908 2203 14 -3.626908541194247 2203 15 -1.4377173091458744 2203 16 -1.633182161753588 2203 17 -0.8825774754346156 2203 18 1.344935752078598 2203 19 0.5761526832955255 2203 20 0.12906273620560427 2203 21 1.4105442176870824 2203 22 -1.3127739984882751 2203 23 0.33012093726379277 2203 24 -0.15147392290248263 2203 25 3.0315570672713505 2204 1 -0.34130763416477805 2204 2 0.9044217687074756 2204 3 -4.8576719576719505 2204 4 0.9452003023431601 2204 5 -0.40415721844293273 2204 6 0.5698790627362058 2204 7 0.8354119425547992 2204 8 -0.7390778533635753 2204 9 -0.3490929705215464 2204 10 1.1834089191232096 2204 11 1.672902494331069 2204 12 1.542970521541946 2204 13 1.6513227513227449 2204 14 3.123091458805753 2204 15 2.8122826908541256 2204 16 2.4501511715797477 2204 17 2.95075585789872 2204 18 3.344935752078598 2204 19 -1.3405139833711388 2204 20 0.71239606953894 2204 21 -0.5894557823129176 2204 22 1.6038926681783892 2204 23 2.0801209372637928 2204 24 1.3485260770975174 2204 25 3.3648904006046862 2205 1 -0.7413076341647766 2205 2 1.1044217687074784 2205 3 0.0423280423280481 2205 4 1.19520030234316 2205 5 -0.9541572184429299 2205 6 -4.980120937263795 2205 7 -0.7645880574452022 2205 8 -0.0390778533635725 2205 9 -1.8990929705215436 2205 10 -0.466591080876789 2205 11 -0.4770975056689295 2205 12 -0.0070294784580511305 2205 13 -0.19867724867725656 2205 14 -0.42690854119424415 2205 15 -3.4377173091458744 2205 16 -1.0998488284202494 2205 17 -1.7492441421012828 2205 18 -0.6550642479214019 2205 19 -6.8905139833711395 2205 20 -1.7376039304610629 2205 21 -1.5894557823129176 2205 22 -2.0461073318216094 2205 23 -0.6698790627362072 2205 24 -0.5514739229024812 2205 25 -1.6351095993953138 2206 1 -1.007974300831446 2206 2 -0.09557823129252441 2206 3 0.30899470899471737 2206 4 1.5285336356764958 2206 5 -0.48749055177626843 2206 6 -0.5134542705971299 2206 7 1.168745275888135 2206 8 1.0942554799697604 2206 9 1.9009070294784536 2206 10 1.6000755857898739 2206 11 1.922902494331069 2206 12 1.1263038548752817 2206 13 2.067989417989409 2206 14 1.373091458805753 2206 15 1.22894935752079 2206 16 2.366817838246412 2206 17 1.7840891912320558 2206 18 2.344935752078598 2206 19 2.5761526832955255 2206 20 -2.2042705971277243 2206 21 -0.2561224489795819 2206 22 1.6872260015117249 2206 23 1.996787603930457 2206 24 2.181859410430853 2206 25 3.0315570672713505 2207 1 -0.591307634164778 2207 2 0.4044217687074756 2207 3 1.6423280423280495 2207 4 1.69520030234316 2207 5 1.3458427815570673 2207 6 1.3198790627362058 2207 7 1.3354119425547992 2207 8 1.2609221466364247 2207 9 1.9009070294784536 2207 10 1.1834089191232096 2207 11 1.922902494331069 2207 12 0.792970521541946 2207 13 1.4013227513227449 2207 14 1.373091458805753 2207 15 1.0622826908541256 2207 16 1.9501511715797477 2207 17 2.20075585789872 2207 18 2.344935752078598 2207 19 2.159486016628861 2207 20 0.96239606953894 2207 21 1.1605442176870824 2207 22 1.3538926681783892 2207 23 1.0801209372637928 2207 24 2.3485260770975174 2207 25 2.6148904006046862 2208 1 0.40869236583522195 2208 2 -0.5955782312925244 2208 3 -0.3576719576719505 2208 4 0.6952003023431601 2208 5 0.8458427815570673 2208 6 1.3198790627362058 2208 7 0.5854119425547992 2208 8 1.0109221466364247 2208 9 -0.8490929705215464 2208 10 -0.31659108087679044 2208 11 0.9229024943310691 2208 12 -0.20702947845805397 2208 13 -0.34867724867725514 2208 14 -0.126908541194247 2208 15 2.5622826908541256 2208 16 -2.5498488284202523 2208 17 0.7007558578987201 2208 18 -3.405064247921402 2208 19 0.4094860166288612 2208 20 -1.03760393046106 2208 21 0.16054421768708238 2208 22 -0.14610733182161084 2208 23 -0.41987906273620723 2208 24 -0.4014739229024826 2208 25 -3.3851095993953138 2209 1 -0.34130763416477805 2209 2 1.3044217687074742 2209 3 2.4423280423280467 2209 4 -1.4047996976568413 2209 5 0.8458427815570673 2209 6 0.41987906273620723 2209 7 1.035411942554802 2209 8 0.9609221466364275 2209 9 0.9009070294784536 2209 10 1.1334089191232124 2209 11 1.5229024943310705 2209 12 1.792970521541946 2209 13 2.201322751322742 2209 14 3.373091458805753 2209 15 1.5622826908541256 2209 16 1.3001511715797491 2209 17 1.8507558578987187 2209 18 2.144935752078595 2209 19 1.7094860166288584 2209 20 2.46239606953894 2209 21 1.0105442176870838 2209 22 0.553892668178392 2209 23 1.5301209372637956 2209 24 1.0485260770975202 2209 25 1.9648904006046877 2210 1 -0.34130763416477805 2210 2 -2.3455782312925244 2210 3 -6.3576719576719505 2210 4 -5.30479969765684 2210 5 -1.9041572184429327 2210 6 -7.430120937263794 2210 7 -0.16458805744520077 2210 8 -10.989077853363575 2210 9 -9.099092970521546 2210 10 -5.56659108087679 2210 11 -7.827097505668931 2210 12 -1.457029478458054 2210 13 -6.598677248677255 2210 14 -6.376908541194247 2210 15 -7.437717309145874 2210 16 -10.549848828420252 2210 17 -6.54924414210128 2210 18 -1.905064247921402 2210 19 -0.8405139833711388 2210 20 -8.53760393046106 2210 21 -13.339455782312918 2210 22 -13.39610733182161 2210 23 -9.419879062736207 2210 24 -5.901473922902483 2210 25 -8.135109599395314 2211 1 0.9920256991685577 2211 2 1.2377551020408113 2211 3 0.9756613756613852 2211 4 0.3618669690098244 2211 5 1.5125094482237316 2211 6 1.48654572940287 2211 7 1.5020786092214635 2211 8 1.427588813303089 2211 9 1.9009070294784536 2211 10 1.9334089191232096 2211 11 1.0895691609977334 2211 12 1.792970521541946 2211 13 2.401322751322745 2211 14 2.7064247921390887 2211 15 3.0622826908541256 2211 16 3.2001511715797477 2211 17 3.1174225245653844 2211 18 2.1782690854119338 2211 19 2.409486016628861 2211 20 2.6290627362056043 2211 21 2.5772108843537467 2211 22 2.0205593348450535 2211 23 2.8301209372637928 2211 24 1.5151927437641817 2211 25 3.5315570672713505 2212 1 1.8586923658352248 2212 2 1.7044217687074728 2212 3 2.242328042328051 2212 4 1.395200302343163 2212 5 1.8458427815570673 2212 6 1.8198790627362058 2212 7 1.8354119425547992 2212 8 1.7609221466364247 2212 9 1.9009070294784536 2212 10 1.9334089191232096 2212 11 1.5229024943310705 2212 12 1.792970521541946 2212 13 2.201322751322742 2212 14 -0.22690854119424841 2212 15 2.5622826908541256 2212 16 2.9001511715797506 2212 17 2.650755857898723 2212 18 3.144935752078595 2212 19 2.5094860166288626 2212 20 3.0623960695389414 2212 21 2.6105442176870852 2212 22 2.553892668178392 2212 23 2.7301209372637913 2212 24 2.8485260770975174 2212 25 3.564890400604689 2213 1 1.658692365835222 2213 2 1.1044217687074784 2213 3 0.6423280423280495 2213 4 1.7952003023431615 2213 5 1.4458427815570687 2213 6 1.2198790627362044 2213 7 0.8354119425547992 2213 8 1.360922146636426 2213 9 1.9009070294784536 2213 10 1.7334089191232067 2213 11 1.7229024943310662 2213 12 1.5929705215419432 2213 13 2.0013227513227463 2213 14 0.5730914588057558 2213 15 1.9622826908541242 2213 16 2.300151171579749 2213 17 2.45075585789872 2213 18 0.5449357520786009 2213 19 2.3094860166288598 2213 20 2.662396069538943 2213 21 2.6105442176870852 2213 22 1.3538926681783892 2213 23 2.3301209372637928 2213 24 0.4485260770975188 2213 25 1.9648904006046877 2214 1 -2.841307634164778 2214 2 -0.26224489795919226 2214 3 0.14232804232804952 2214 4 -3.138133030990172 2214 5 -3.6541572184429327 2214 6 -0.6801209372637942 2214 7 -3.6645880574452008 2214 8 -0.40574452003023964 2214 9 -2.5990929705215464 2214 10 -0.06659108087679044 2214 11 -0.9104308390022666 2214 12 1.1263038548752817 2214 13 -1.7653439153439194 2214 14 -0.9602418745275827 2214 15 0.22894935752078993 2214 16 0.2001511715797477 2214 17 -2.215910808767944 2214 18 -1.8217309145880662 2214 19 -4.757180650037803 2214 20 -0.03760393046106003 2214 21 -4.256122448979582 2214 22 0.18722600151172486 2214 23 -2.6698790627362072 2214 24 -0.15147392290248263 2214 25 -0.9684429327286495 2215 1 0.15869236583522195 2215 2 1.1544217687074756 2215 3 0.14232804232804952 2215 4 1.69520030234316 2215 5 -2.1541572184429327 2215 6 0.8198790627362058 2215 7 0.8354119425547992 2215 8 -0.23907785336357534 2215 9 -1.3490929705215464 2215 10 0.9334089191232096 2215 11 0.17290249433106908 2215 12 -2.207029478458054 2215 13 -0.34867724867725514 2215 14 -5.376908541194247 2215 15 -0.9377173091458744 2215 16 0.9501511715797477 2215 17 0.4507558578987201 2215 18 0.34493575207859806 2215 19 -1.3405139833711388 2215 20 1.71239606953894 2215 21 1.1605442176870824 2215 22 1.3538926681783892 2215 23 0.33012093726379277 2215 24 0.5985260770975174 2215 25 1.1148904006046862 2216 1 0.658692365835222 2216 2 -0.09557823129252441 2216 3 1.1423280423280495 2216 4 0.6952003023431601 2216 5 -1.4041572184429327 2216 6 0.8198790627362058 2216 7 1.0854119425547992 2216 8 0.26092214663642466 2216 9 -1.8490929705215464 2216 10 0.43340891912320956 2216 11 -0.8270975056689309 2216 12 0.04297052154194603 2216 13 -1.0986772486772551 2216 14 -1.376908541194247 2216 15 1.0622826908541256 2216 16 2.4501511715797477 2216 17 1.70075585789872 2216 18 2.344935752078598 2216 19 1.9094860166288612 2216 20 -0.78760393046106 2216 21 0.6605442176870824 2216 22 2.103892668178389 2216 23 1.0801209372637928 2216 24 0.5985260770975174 2216 25 0.8648904006046862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 1.0 14 2.0 15 4.0 16 7.0 17 7.0 18 7.0 19 19.0 20 12.0 21 13.0 22 10.0 23 12.0 24 13.0 25 15.0 26 19.0 27 13.0 28 20.0 29 20.0 30 25.0 31 46.0 32 68.0 33 76.0 34 126.0 35 192.0 36 329.0 37 550.0 38 1103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33038021410115 19.416758951642674 11.627906976744185 23.624953857511997 2 14.902250092216896 22.279601623017335 38.25156768720029 24.566580597565473 3 19.218000737735153 28.070822574695686 30.911102914053856 21.800073773515308 4 13.943194393212838 20.066396163777203 32.05459240132792 33.935817041682036 5 11.545555145702693 38.84175580966433 34.23091110291405 15.381777941718921 6 30.13648100331981 33.308742161564 20.877904832165253 15.67687200295094 7 23.12799704905939 35.669494651420145 21.578753227591292 19.62375507192918 8 24.566580597565473 33.56694946514202 22.35337513832534 19.51309479896717 9 27.148653633345628 18.701586130579123 19.918849133161196 34.23091110291405 10 16.267060125414975 26.890446329767613 31.50129103651789 25.34120250829952 11 30.431575064551826 26.742899299151606 20.066396163777203 22.759129472519366 12 25.193655477683514 26.779786056805605 27.812615271117668 20.213943194393213 13 30.21025451862781 21.578753227591292 28.329029878273698 19.881962375507193 14 25.119881962375505 25.230542235337516 25.230542235337516 24.419033566949466 15 29.472519365547768 23.238657322021396 26.11582441903357 21.17299889339727 16 22.722242714865363 25.67318332718554 30.0258207303578 21.578753227591292 17 20.472150497971228 31.058649944669863 26.816672814459608 21.6525267428993 18 21.6156399852453 22.796016230173368 34.30468461822206 21.28365916635928 19 22.132054592401328 30.39468830689782 27.259313906307636 20.213943194393213 20 21.468092954629288 26.964219845075615 31.390630763555883 20.17705643673921 21 24.640354112873478 28.43969015123571 25.34120250829952 21.578753227591292 22 21.50497971228329 26.595352268535592 26.484691995573588 25.414976023607526 23 24.898561416451496 23.3493175949834 26.410918480265583 25.34120250829952 24 21.320545924013278 27.48063445223165 26.374031722611583 24.824787901143488 25 24.714127628181483 27.62818148284766 25.636296569531535 22.02139431943932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.0 2 4.5 3 6.0 4 6.0 5 6.0 6 12.0 7 18.0 8 18.0 9 18.0 10 16.5 11 15.0 12 15.0 13 15.0 14 15.0 15 15.0 16 15.0 17 15.0 18 33.5 19 52.0 20 52.0 21 52.0 22 54.5 23 57.0 24 57.0 25 57.0 26 77.5 27 98.0 28 98.0 29 98.0 30 128.5 31 159.0 32 159.0 33 159.0 34 166.0 35 173.0 36 173.0 37 173.0 38 197.0 39 221.0 40 221.0 41 221.0 42 363.5 43 506.0 44 506.0 45 506.0 46 457.0 47 408.0 48 408.0 49 408.0 50 372.5 51 337.0 52 337.0 53 337.0 54 264.5 55 192.0 56 192.0 57 192.0 58 180.5 59 169.0 60 169.0 61 169.0 62 144.5 63 120.0 64 120.0 65 120.0 66 93.5 67 67.0 68 67.0 69 67.0 70 58.0 71 49.0 72 49.0 73 49.0 74 32.5 75 16.0 76 16.0 77 16.0 78 18.0 79 20.0 80 20.0 81 20.0 82 13.5 83 7.0 84 7.0 85 7.0 86 4.0 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07377351530800443 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2711.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.15049797122833 #Duplication Level Percentage of deduplicated Percentage of total 1 88.8036809815951 64.07229804500184 2 6.952965235173824 10.033198081888601 3 1.2269938650306749 2.6558465510881595 4 0.6134969325153374 1.770564367392106 5 0.6134969325153374 2.213205459240133 6 0.4601226993865031 1.9918849133161196 7 0.10224948875255625 0.5164146071560309 8 0.10224948875255625 0.5901881224640354 9 0.10224948875255625 0.6639616377720399 >10 0.9713701431492843 12.947251936554776 >50 0.051124744376278126 2.5451862781261525 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 69 2.5451862781261525 No Hit CTTATGTACTCTGCGTTGATACCAC 36 1.3279232755440797 No Hit GTATCAACGCAGAGTACTTTTTTTT 36 1.3279232755440797 No Hit GCTTATGTACTCTGCGTTGATACCA 34 1.2541497602360752 No Hit GGTATCAACGCAGAGTACTTTTTTT 27 0.9959424566580598 No Hit CCCATGTACTCTGCGTTGATACCAC 24 0.885282183696053 No Hit TATGTACTCTGCGTTGATACCACTG 22 0.8115086683880488 No Hit GTGGTATCAACGCAGAGTACATGGG 18 0.6639616377720399 No Hit CATAAGCAGTGGTATCAACGCAGAG 18 0.6639616377720399 No Hit GAGTACATAAGCAGTGGTATCAACG 18 0.6639616377720399 No Hit GCGTTGATACCACTGCTTATGTACT 14 0.5164146071560309 No Hit GTATCAACGCAGAGTACATAAGCAG 13 0.47952784950202876 No Hit CATGTACTCTGCGTTGATACCACTG 13 0.47952784950202876 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 12 0.4426410918480265 No Hit TATCAACGCAGAGTACTTTTTTTTT 12 0.4426410918480265 No Hit ACTCTGCGTTGATACCACTGCTTAT 11 0.4057543341940244 No Hit CTGCTTATGTACTCTGCGTTGATAC 11 0.4057543341940244 No Hit TATCAACGCAGAGTACATAAGCAGT 11 0.4057543341940244 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11 0.4057543341940244 No Hit ATCATGTACTCTGCGTTGATACCAC 10 0.3688675765400221 No Hit AAGCAGTGGTATCAACGCAGAGTAC 9 0.33198081888601993 No Hit GCAGTGGTATCAACGCAGAGTACAT 9 0.33198081888601993 No Hit GGTATCAACGCAGAGTACATAAGCA 8 0.2950940612320177 No Hit GGTATCAACGCAGAGTACATGGGGG 8 0.2950940612320177 No Hit GAGTACATGATAAGCAGTGGTATCA 7 0.25820730357801547 No Hit ATCAACGCAGAGTACTTTTTTTTTT 7 0.25820730357801547 No Hit ATACAGGGTGACAGCCCCGTACACA 6 0.22132054592401326 No Hit ACGCAGAGTACATAAGCAGTGGTAT 6 0.22132054592401326 No Hit GTACTGGTTCACTATCGGTCAGTCA 6 0.22132054592401326 No Hit GGTATCAACGCAGAGTACATGGGAA 6 0.22132054592401326 No Hit GTGGTATCAACGCAGAGTACTTTTT 6 0.22132054592401326 No Hit GGTTAATGAGGCGAACCGGGGGAAC 6 0.22132054592401326 No Hit GATACCACTGCTTATGTACTCTGCG 6 0.22132054592401326 No Hit GCTTATCATGTACTCTGCGTTGATA 6 0.22132054592401326 No Hit GCTTATCCCATGTACTCTGCGTTGA 6 0.22132054592401326 No Hit ACATAAGCAGTGGTATCAACGCAGA 5 0.18443378827001106 No Hit GTATCAACGCAGAGTACATGGGGTG 5 0.18443378827001106 No Hit GTATCAACGCAGAGTACATGGGGGT 5 0.18443378827001106 No Hit GTACATGGTAAGCAGTGGTATCAAC 5 0.18443378827001106 No Hit ACTCTGCGTTGATACCACTGCTTAC 5 0.18443378827001106 No Hit CCACTGCTTATGTACTCTGCGTTGA 5 0.18443378827001106 No Hit GTACATGGGGGTTAAGCGACTAAGC 5 0.18443378827001106 No Hit GTACATGGGAAGCAGTGGTATCAAC 5 0.18443378827001106 No Hit GTACATGGGATAAGCAGTGGTATCA 5 0.18443378827001106 No Hit GGATAAGCAGTGGTATCAACGCAGA 5 0.18443378827001106 No Hit GTGCTAATCTGCGATAAGCGTCGGT 5 0.18443378827001106 No Hit GTGGTATCAACGCAGAGTACATAAG 5 0.18443378827001106 No Hit GTACTTAGATGTTTCAGTTCCCCCG 4 0.14754703061600885 No Hit CCCCATGTACTCTGCGTTGATACCA 4 0.14754703061600885 No Hit GAGTACATGGGATAAGCAGTGGTAT 4 0.14754703061600885 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 4 0.14754703061600885 No Hit CTTCCCATGTACTCTGCGTTGATAC 4 0.14754703061600885 No Hit GGATACCACGTGTCCCGCCCTACTC 4 0.14754703061600885 No Hit GTTAATGATAGTGTGTCGAAACACA 4 0.14754703061600885 No Hit GTTGATACCACTGCTTATGTACTCT 4 0.14754703061600885 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4 0.14754703061600885 No Hit GGGTTTCCCCATTCGGAAATCGCCG 4 0.14754703061600885 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4 0.14754703061600885 No Hit TAAGTACCCCGAGGAAAAGAAATCA 4 0.14754703061600885 No Hit TATCAACGCAGAGTACATGGGAAGC 3 0.11066027296200663 No Hit ACCATGTACTCTGCGTTGATACCAC 3 0.11066027296200663 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 3 0.11066027296200663 No Hit TATCAACGCAGAGTACATGGGGGTT 3 0.11066027296200663 No Hit CAGTGGTATCAACGCAGAGTACATA 3 0.11066027296200663 No Hit CAGTGGTATCAACGCAGAGTACATG 3 0.11066027296200663 No Hit CTCCTGCAGTGACCGAAATGCAAAT 3 0.11066027296200663 No Hit ATAGTGTGTCGAAACACACTGGGTT 3 0.11066027296200663 No Hit TACCACTGCTTATGTACTCTGCGTT 3 0.11066027296200663 No Hit CATCTAAGTACCCCGAGGAAAAGAA 3 0.11066027296200663 No Hit GGGTACTTAGATGTTTCAGTTCCCC 3 0.11066027296200663 No Hit GCCTTGGAGGATGGTCCCCCCATAT 3 0.11066027296200663 No Hit CATGATAAGCAGTGGTATCAACGCA 3 0.11066027296200663 No Hit GGTATCAACGCAGAGTACATGGGGT 3 0.11066027296200663 No Hit GGTTCACTATCGGTCAGTCAGGAGT 3 0.11066027296200663 No Hit AGCGTACACGGTGGATGCCCTGGCA 3 0.11066027296200663 No Hit GAGTACATGGGAAGCAGTGGTATCA 3 0.11066027296200663 No Hit TACCACTGCTTATCATGTACTCTGC 3 0.11066027296200663 No Hit GTATCAACGCAGAGTACATGGGAAG 3 0.11066027296200663 No Hit CATTAACTGAATCCATAGGTTAATG 3 0.11066027296200663 No Hit AAAGTACTCTGCGTTGATACCACTG 3 0.11066027296200663 No Hit TGCTTATGTACTCTGCGTTGATACC 3 0.11066027296200663 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 3 0.11066027296200663 No Hit GTATCAACGCAGAGTACATGGGATA 3 0.11066027296200663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.03688675765400221 0.0 11 0.0 0.0 0.0 0.03688675765400221 0.0 12 0.0 0.0 0.0 0.03688675765400221 0.0 13 0.0 0.0 0.0 0.03688675765400221 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE