##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6897 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.50862693924895 31.0 30.0 33.0 27.0 34.0 2 30.433231839930404 31.0 30.0 34.0 26.0 34.0 3 30.66115702479339 31.0 31.0 34.0 27.0 34.0 4 33.96853704509207 35.0 35.0 37.0 28.0 37.0 5 34.19501232419893 37.0 35.0 37.0 30.0 37.0 6 34.36697114687545 37.0 35.0 37.0 30.0 37.0 7 34.13063650862694 37.0 35.0 37.0 29.0 37.0 8 34.318399304045236 37.0 35.0 37.0 30.0 37.0 9 35.24865883717558 39.0 35.0 39.0 27.0 39.0 10 35.221835580687255 39.0 35.0 39.0 28.0 39.0 11 35.83021603595766 39.0 35.0 39.0 30.0 39.0 12 35.79570827896187 39.0 35.0 39.0 30.0 39.0 13 35.77381470204437 39.0 35.0 39.0 30.0 39.0 14 36.49862258953168 40.0 36.0 41.0 27.0 41.0 15 36.780339277946936 40.0 36.0 41.0 29.0 41.0 16 36.83210091344063 40.0 36.0 41.0 30.0 41.0 17 36.450485718428304 39.0 36.0 41.0 27.0 41.0 18 36.40060896041757 39.0 36.0 41.0 27.0 41.0 19 36.823691460055095 40.0 36.0 41.0 30.0 41.0 20 37.0172538784979 40.0 37.0 41.0 30.0 41.0 21 36.98100623459475 40.0 37.0 41.0 30.0 41.0 22 36.8145570537915 40.0 36.0 41.0 29.0 41.0 23 36.42322749021314 39.0 36.0 41.0 27.0 41.0 24 36.792663476874004 40.0 36.0 41.0 29.0 41.0 25 35.61939973901696 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9130150713484007 1101 2 0.6458768744482981 1101 3 -0.70132499775357 1101 4 -0.013890606747757772 1101 5 0.004572147429286133 1101 6 1.1645419835896007 1101 7 1.6860121360121383 1101 8 0.8647404623595136 1101 9 2.1225751320989374 1101 10 3.205712100950194 1101 11 0.9639148329624518 1101 12 1.3160900827567303 1101 13 1.7040363516553896 1101 14 2.2680510406700876 1101 15 1.5250007928579379 1101 16 0.35754417540132266 1101 17 1.7994631470821787 1101 18 2.275422417089082 1101 19 1.8164003985432586 1101 20 0.5712315021838847 1101 21 2.0385380052046713 1101 22 2.3060836870360575 1101 23 1.8702110940206254 1101 24 1.3226307642974362 1101 25 2.2662872400967515 1102 1 -2.175873817540488 1102 2 -1.354123125551702 1102 3 -1.6013249977535686 1102 4 -1.247223940081092 1102 5 -1.5287611859040524 1102 6 -1.6910135719659536 1102 7 -2.3473211973211967 1102 8 -2.224148426529375 1102 9 -1.4885359790121768 1102 10 -1.2053990101609173 1102 11 -1.2916407225931081 1102 12 -1.8950210283543854 1102 13 -1.818185870566829 1102 14 -1.5541711815521353 1102 15 -1.0083325404754007 1102 16 -2.0757891579320145 1102 17 -4.28942574180671 1102 18 -2.0023553606886964 1102 19 -1.3502662681234057 1102 20 -0.8398796089272267 1102 21 -1.3059064392397701 1102 22 -2.805027424075057 1102 23 -2.307566683757152 1102 24 -1.988480346813681 1102 25 -5.911490537681026 1103 1 0.04634840468173351 1103 2 0.8958768744482981 1103 3 0.9820083355797635 1103 4 1.502776059918908 1103 5 0.387905480762619 1103 6 -1.4687913497437322 1103 7 -0.6806545306545289 1103 8 -0.8352595376404892 1103 9 -2.294091534567727 1103 10 -0.7609545657164674 1103 11 0.013914832962448997 1103 12 0.7994234160900646 1103 13 -0.6792969816779433 1103 14 -3.248615625996578 1103 15 -1.3416658738087293 1103 16 -1.909122491265343 1103 17 -1.3172035195844884 1103 18 -1.4745775829109178 1103 19 -1.266932934790077 1103 20 0.18789816885055188 1103 21 -0.5281286614619916 1103 22 0.22275035370272178 1103 23 1.5535444273539625 1103 24 1.6226307642974334 1103 25 -2.383712759903247 1104 1 0.5591689175022481 1104 2 0.5689537975252215 1104 3 1.0140596176310481 1104 4 0.47072477786763045 1104 5 1.0353413781985168 1104 6 0.018388137435756846 1104 7 1.0629352129352156 1104 8 1.5878173854364306 1104 9 0.930267439791244 1104 10 1.3672505624886568 1104 11 1.263914832962449 1104 12 0.8314746981413421 1104 13 2.0194209670400056 1104 14 3.2065125791316262 1104 15 1.4019238697810152 1104 16 2.2575441754013212 1104 17 2.0610016086206357 1104 18 2.9292685709352355 1104 19 2.2394773216201784 1104 20 1.502000732953121 1104 21 1.753922620589286 1104 22 1.1522375331899042 1104 23 1.1625187863283202 1104 24 1.7380153796820466 1104 25 2.327825701635213 1105 1 -0.5369849286515986 1105 2 1.145876874448298 1105 3 -0.7679916644202365 1105 4 0.752776059918908 1105 5 0.7212388140959476 1105 6 1.5312086502562678 1105 7 0.9860121360121354 1105 8 1.4147404623595108 1105 9 -0.6274248679010626 1105 10 1.905712100950197 1105 11 1.9305814996291133 1105 12 2.382756749423393 1105 13 2.9040363516553924 1105 14 0.6680510406700861 1105 15 1.9916674595245993 1105 16 1.5075441754013212 1105 17 -1.9005368529178241 1105 18 1.9420890837557465 1105 19 1.983067065209923 1105 20 1.5212315021838876 1105 21 2.1385380052046727 1105 22 2.139417020369393 1105 23 3.0535444273539625 1105 24 2.955964097630769 1105 25 4.116287240096753 1106 1 -0.08698492865159935 1106 2 1.2458768744482995 1106 3 0.4986750022464328 1106 4 -2.5138906067477578 1106 5 -1.8954278525707196 1106 6 -3.735458016410398 1106 7 -3.213987863987864 1106 8 -0.7352595376404878 1106 9 -1.8774248679010626 1106 10 0.7057121009501941 1106 11 -1.4360851670375538 1106 12 -1.8839099172432725 1106 13 -1.695963648344609 1106 14 -0.4319489593299153 1106 15 -1.7749992071420664 1106 16 -0.9424558245986816 1106 17 -0.3005368529178227 1106 18 0.1754224170890808 1106 19 -0.18359960145674137 1106 20 -1.0287684978161096 1106 21 -0.16146199479532442 1106 22 -1.9939163129639397 1106 23 -0.029788905979373226 1106 24 0.4226307642974305 1106 25 0.5662872400967558 1107 1 -1.979292620959292 1107 2 -1.7387385101670851 1107 3 0.24482884840027808 1107 4 -0.8369675298246761 1107 5 -0.8877355448784101 1107 6 -2.5969964779488564 1107 7 -0.706295556295558 1107 8 0.280125077744124 1107 9 -0.14665563713182905 1107 10 0.44417363941173704 1107 11 1.0331456021932155 1107 12 -1.4762176095509645 1107 13 -2.4421174944984543 1107 14 0.43728180990085264 1107 15 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0.9535691821406047 1109 3 1.3986750022464314 1109 4 2.0091863163291634 1109 5 2.18918753204467 1109 6 2.095311214358837 1109 7 1.0629352129352156 1109 8 1.4339712315902773 1109 9 2.314882824406631 1109 10 0.21340440864250354 1109 11 0.5716071406547556 1109 12 1.9853208519874954 1109 13 1.4809594285784726 1109 14 2.8988202714393196 1109 15 1.6326931005502416 1109 16 2.3344672523244014 1109 17 2.2917708393898693 1109 18 2.3908070324736954 1109 19 2.085631167774025 1109 20 1.5789238098761942 1109 21 0.5231533898200595 1109 22 2.3060836870360575 1109 23 1.8548264786360136 1109 24 2.2764769181435867 1109 25 2.7893641631736728 1110 1 1.531196889530218 1110 2 -0.44503221646079183 1110 3 0.03503863861006806 1110 4 -0.3684360612932096 1110 5 0.6227539656111034 1110 6 0.13726925631687692 1110 7 0.8041939541939556 1110 8 1.5738313714504173 1110 9 0.44075695028075756 1110 10 0.2693484645865638 1110 11 0.7184602875079023 1110 12 1.5797264463930958 1110 13 -1.1414181937991543 1110 14 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0.9385380052046699 2207 22 1.1060836870360546 2207 23 0.4702110940206268 2207 24 0.12263076429743336 2207 25 1.5662872400967558 2208 1 1.6221059804393114 2208 2 1.827695056266478 2208 3 1.3986750022464314 2208 4 1.8133821205249703 2208 5 2.0772994201565567 2208 6 1.6827238017714237 2208 7 1.8041939541939556 2208 8 0.3011040987231439 2208 9 0.1680296775534842 2208 10 1.6329848282229236 2208 11 2.445733014780629 2208 12 0.852453719120362 2208 13 0.04039998801902556 2208 14 -0.6046762320571872 2208 15 -0.03863557077843183 2208 16 1.4393623572195011 2208 17 3.054008601627629 2208 18 2.775422417089082 2208 19 2.407309489452352 2208 20 2.4530496840020675 2208 21 2.6839925506592195 2208 22 0.9424473233996906 2208 23 1.1974838212933534 2208 24 1.9408125824792535 2208 25 2.1390145128240263 2209 1 0.3130150713484028 2209 2 -0.9541231255517033 2209 3 -0.9013249977535693 2209 4 -0.013890606747757772 2209 5 -0.9954278525707139 2209 6 -0.13545801641039645 2209 7 -0.8139878639878617 2209 8 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1.7754224170890822 2213 19 1.41640039854326 2213 20 -0.22876849781611241 2213 21 0.7385380052046742 2213 22 1.706083687036056 2213 23 -0.3297889059793704 2213 24 -0.07736923570256948 2213 25 0.7662872400967515 2214 1 1.2130150713484014 2214 2 1.645876874448298 2214 3 1.4986750022464328 2214 4 0.18610939325224507 2214 5 2.204572147429282 2214 6 0.1645419835896007 2214 7 0.886012136012134 2214 8 0.2647404623595122 2214 9 0.4225751320989346 2214 10 0.5057121009501984 2214 11 0.263914832962449 2214 12 0.1160900827567275 2214 13 1.804036351655391 2214 14 3.368051040670089 2214 15 0.5250007928579379 2214 16 1.957544175401324 2214 17 -0.7005368529178213 2214 18 -0.4245775829109206 2214 19 0.5164003985432615 2214 20 0.4712315021838904 2214 21 1.1385380052046727 2214 22 2.206083687036056 2214 23 -0.029788905979373226 2214 24 0.3226307642974362 2214 25 0.1662872400967501 2215 1 -3.0369849286515986 2215 2 -1.437456458885034 2215 3 -0.5179916644202365 2215 4 -0.24722394008109205 2215 5 0.2212388140959476 2215 6 -0.46879134974373216 2215 7 -1.1806545306545289 2215 8 -5.668592870973821 2215 9 -2.294091534567727 2215 10 0.48904543428353264 2215 11 -1.4027518337042153 2215 12 -0.20057658390993538 2215 13 -0.4292969816779433 2215 14 -1.2486156259965782 2215 15 -3.5083325404754007 2215 16 -3.0757891579320145 2215 17 -0.06720351958448845 2215 18 -2.641244249577582 2215 19 -2.016932934790077 2215 20 -2.812101831149448 2215 21 -1.1114619947953273 2215 22 -2.4439163129639425 2215 23 -3.5297889059793732 2215 24 -1.2107025690359023 2215 25 -2.383712759903247 2216 1 -5.9536515953182665 2216 2 -8.354123125551702 2216 3 -6.601324997753569 2216 4 -8.413890606747756 2216 5 -5.695427852570717 2216 6 -4.968791349743729 2216 7 -8.347321197321197 2216 8 -6.501926204307157 2216 9 -6.3774248679010626 2216 10 -5.760954565716471 2216 11 -6.902751833704219 2216 12 -9.450576583909939 2216 13 -8.262630315011275 2216 14 -8.665282292663246 2216 15 -7.674999207142065 2216 16 -7.575789157932011 2216 17 -6.067203519584492 2216 18 -7.05791091624425 2216 19 -9.35026626812341 2216 20 -7.89543516448278 2216 21 -9.028128661461995 2216 22 -7.86058297963061 2216 23 -7.696455572646041 2216 24 -11.544035902369234 2216 25 -8.800379426569915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 4.0 4 0.0 5 0.0 6 0.0 7 4.0 8 1.0 9 1.0 10 4.0 11 5.0 12 13.0 13 21.0 14 24.0 15 28.0 16 28.0 17 39.0 18 32.0 19 46.0 20 37.0 21 29.0 22 36.0 23 46.0 24 40.0 25 52.0 26 35.0 27 52.0 28 64.0 29 86.0 30 116.0 31 153.0 32 197.0 33 252.0 34 393.0 35 570.0 36 754.0 37 1267.0 38 2449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.77196804647785 21.539578794480754 13.725490196078432 22.962962962962962 2 15.749636098981076 21.746724890829693 39.461426491994175 23.04221251819505 3 18.01368865589049 26.05213339158293 30.52279015581768 25.411387796708894 4 12.993445010924981 18.033503277494535 38.4122359796067 30.56081573197378 5 14.319009468317553 37.494537509104156 34.11507647487254 14.071376547705755 6 31.110787596447807 38.85572863590042 16.712767506187216 13.320716261464552 7 26.335711166108606 33.04702285631097 23.031008880477508 17.586257097102926 8 24.03202328966521 36.695778748180494 20.902474526928675 18.36972343522562 9 24.938127820643473 16.08676663269763 22.92910176153734 36.04600378512156 10 14.76842411884649 29.347509466938536 33.76055927759977 22.123507136615206 11 32.057625145518045 25.305587892898718 23.850407450523864 18.78637951105937 12 21.560634735769398 27.573154753239194 31.620323191148643 19.245887319842772 13 26.521106259097525 24.046579330422123 26.21542940320233 23.21688500727802 14 19.92721979621543 24.075691411935953 28.26783114992722 27.729257641921397 15 22.087944088526502 30.794991263832266 24.796156086196856 22.32090856144438 16 22.25618631732169 30.698689956331876 25.633187772925766 21.41193595342067 17 20.611798980335035 29.90531682447196 28.346686088856522 21.13619810633649 18 20.133935070607077 29.713204250982677 29.6404134517397 20.51244722667055 19 20.62891250545931 28.519435143397875 29.69864609113408 21.153006260008738 20 23.147474159266267 27.92255058960547 28.79604018052118 20.133935070607077 21 22.972776241083125 29.028970738098703 26.888921240355216 21.109331780462952 22 21.088633386697715 29.224275942366468 29.035074952699752 20.652015718236065 23 22.61350407450524 29.36554132712456 27.750291036088477 20.270663562281722 24 22.07017033047023 27.500363953996214 29.334692094919202 21.094773620614355 25 22.81722933643772 29.17636786961583 27.41559953434226 20.59080325960419 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24.0 1 24.0 2 19.5 3 15.0 4 15.0 5 15.0 6 15.5 7 16.0 8 16.0 9 16.0 10 19.0 11 22.0 12 22.0 13 22.0 14 42.0 15 62.0 16 62.0 17 62.0 18 92.0 19 122.0 20 122.0 21 122.0 22 180.0 23 238.0 24 238.0 25 238.0 26 332.5 27 427.0 28 427.0 29 427.0 30 503.5 31 580.0 32 580.0 33 580.0 34 629.5 35 679.0 36 679.0 37 679.0 38 762.5 39 846.0 40 846.0 41 846.0 42 841.0 43 836.0 44 836.0 45 836.0 46 803.0 47 770.0 48 770.0 49 770.0 50 744.0 51 718.0 52 718.0 53 718.0 54 640.5 55 563.0 56 563.0 57 563.0 58 472.0 59 381.0 60 381.0 61 381.0 62 350.0 63 319.0 64 319.0 65 319.0 66 222.5 67 126.0 68 126.0 69 126.0 70 104.5 71 83.0 72 83.0 73 83.0 74 62.5 75 42.0 76 42.0 77 42.0 78 30.0 79 18.0 80 18.0 81 18.0 82 13.0 83 8.0 84 8.0 85 8.0 86 4.5 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17398869073510223 2 0.39147455415397997 3 0.4349717268377556 4 0.46396984196027263 5 0.46396984196027263 6 0.40597361171523855 7 0.40597361171523855 8 0.39147455415397997 9 0.40597361171523855 10 0.44947078439901406 11 0.3624764390314629 12 0.40597361171523855 13 0.39147455415397997 14 0.39147455415397997 15 0.420472669276497 16 0.39147455415397997 17 0.46396984196027263 18 0.40597361171523855 19 0.40597361171523855 20 0.40597361171523855 21 0.40597361171523855 22 0.37697549659272145 23 0.3624764390314629 24 0.40597361171523855 25 0.3624764390314629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6897.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.07162534435263 #Duplication Level Percentage of deduplicated Percentage of total 1 98.92075694855116 97.01319414238074 2 0.8131283264340627 1.5948963317384368 3 0.11827321111768185 0.34797738147020446 4 0.044352454169130695 0.17398869073510223 5 0.0 0.0 6 0.014784151389710232 0.08699434536755112 7 0.044352454169130695 0.3044802087864289 8 0.0 0.0 9 0.0 0.0 >10 0.044352454169130695 0.4784688995215311 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCATTCCATTCCATTCCATTCC 11 0.1594896331738437 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 11 0.1594896331738437 No Hit GTGGTATCAACGCAGAGTACATGGG 11 0.1594896331738437 No Hit GGTATCAACGCAGAGTACTTTTTTT 7 0.10149340292880964 No Hit GTATCAACGCAGAGTACTTTTTTTT 7 0.10149340292880964 No Hit GAATGGAATGGAATGGAATGGAATG 7 0.10149340292880964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE