##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6897 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.348992315499494 31.0 31.0 33.0 28.0 34.0 2 31.470204436711615 31.0 31.0 34.0 28.0 34.0 3 31.60069595476294 31.0 31.0 34.0 28.0 34.0 4 35.191097578657384 37.0 35.0 37.0 32.0 37.0 5 35.29317094388865 37.0 35.0 37.0 33.0 37.0 6 35.2257503262288 37.0 35.0 37.0 32.0 37.0 7 35.14760040597361 37.0 35.0 37.0 32.0 37.0 8 35.107872988255764 37.0 35.0 37.0 32.0 37.0 9 36.852544584602 39.0 37.0 39.0 33.0 39.0 10 36.8697984630999 39.0 37.0 39.0 33.0 39.0 11 36.88429752066116 39.0 37.0 39.0 33.0 39.0 12 36.81383210091344 39.0 37.0 39.0 32.0 39.0 13 36.701174423662465 39.0 37.0 39.0 32.0 39.0 14 37.925184862983905 40.0 38.0 41.0 33.0 41.0 15 37.92156009859359 40.0 38.0 41.0 32.0 41.0 16 38.04219225750326 40.0 38.0 41.0 33.0 41.0 17 37.97506162099464 40.0 38.0 41.0 32.0 41.0 18 37.93170943888647 40.0 38.0 41.0 33.0 41.0 19 38.04596201246919 40.0 38.0 41.0 32.0 41.0 20 37.989850659707116 40.0 38.0 41.0 32.0 41.0 21 37.97100188487748 40.0 38.0 41.0 32.0 41.0 22 37.92924459910106 40.0 38.0 41.0 32.0 41.0 23 37.903291286066406 40.0 38.0 41.0 32.0 41.0 24 37.97477163984341 40.0 38.0 41.0 32.0 41.0 25 36.82282151660142 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14873697730840263 1101 2 0.42964328440518784 1101 3 0.7645712312378983 1101 4 0.5444849682944835 1101 5 -0.054735299973380336 1101 6 -0.7066960376484133 1101 7 -1.7693618991238154 1101 8 -1.9695585278918628 1101 9 0.01117841594033564 1101 10 -3.5512991417753383 1101 11 -0.0075006122625183025 1101 12 -2.3417499607975856 1101 13 -0.7775141965618246 1101 14 -1.2693566134042413 1101 15 -0.7717758432044164 1101 16 -1.3480649421125577 1101 17 0.1462420295753475 1101 18 0.48974266474267125 1101 19 -0.6059239702096804 1101 20 0.49512841655700157 1101 21 -0.851811019668169 1101 22 -0.028864741959964135 1101 23 -0.02924293519531318 1101 24 0.7693610181705424 1101 25 -3.1031280447947154 1102 1 0.9820703106417383 1102 2 0.9740877288496321 1102 3 0.6867934534601225 1102 4 0.7333738571833663 1102 5 0.8563758111377311 1102 6 0.2377484067960296 1102 7 0.4861936564317446 1102 8 0.8193303609970215 1102 9 -0.2110438062818858 1102 10 0.37092308044688593 1102 11 -2.018611723373631 1102 12 0.9138055947579744 1102 13 0.8780413589937339 1102 14 0.7861989421513158 1102 15 -1.2051091765377535 1102 16 0.8074906134430009 1102 17 0.5906864740197904 1102 18 -0.04359066859066729 1102 19 -1.3392573035430146 1102 20 -0.1382049167763313 1102 21 0.9148556469984968 1102 22 1.7155797024844759 1102 23 -0.7181318240841961 1102 24 1.3915832403927624 1102 25 0.9413163996497289 1103 1 0.6487369773084026 1103 2 1.112976617738525 1103 3 1.1312378979045654 1103 4 0.5944849682944806 1103 5 0.6619313666932811 1103 6 1.3766372956849224 1103 7 1.5973047675428518 1103 8 1.2637748054414715 1103 9 1.427845082607 1103 10 1.5653675248913288 1103 11 1.0924993877374831 1103 12 1.941583372535753 1103 13 1.8224858034381768 1103 14 2.4806433865957587 1103 15 1.8782241567955822 1103 16 1.7519350578874437 1103 17 0.9795753629086832 1103 18 1.5397426647426684 1103 19 1.9107426964569854 1103 20 2.4451284165570044 1103 21 1.4148556469984968 1103 22 1.771135258040033 1103 23 1.1707570648046897 1103 24 1.2526943515038766 1103 25 1.6635386218719503 1104 1 0.4179677465391727 1104 2 -0.5472797925178874 1104 3 -0.9200441533774821 1104 4 -0.463207339397826 1104 5 0.4375723923343102 1104 6 1.2548424238900466 1104 7 1.238330408568494 1104 8 1.3919799336465957 1104 9 0.7804091851711021 1104 10 0.8410085505323579 1104 11 1.4001916954297897 1104 12 0.5890192699716508 1104 13 0.7071011880535636 1104 14 -0.15397199801962813 1104 15 0.512839541410969 1104 16 1.0980889040412904 1104 17 1.1462420295753475 1104 18 1.4435888185888217 1104 19 0.45561449132878096 1104 20 0.7335899550185445 1104 21 -0.9056571735143208 1104 22 0.8095967965015731 1104 23 1.4015262955739232 1104 24 1.8616687104782343 1104 25 0.11225657058989924 1105 1 0.39873697730840263 1105 2 0.7796432844051893 1105 3 0.4645712312379011 1105 4 1.1778183016278163 1105 5 0.9952647000266168 1105 6 0.959970629018251 1105 7 0.3473047675428518 1105 8 1.0971081387748 1105 9 1.5945117492736713 1105 10 1.7320341915580002 1105 11 1.3424993877374831 1105 12 -0.808416627464247 1105 13 1.3224858034381768 1105 14 1.3139767199290944 1105 15 0.7948908234622465 1105 16 -1.831398275445892 1105 17 -0.10375797042465251 1105 18 -0.5435906685906673 1105 19 1.1607426964569854 1105 20 1.6951284165570044 1105 21 1.3315223136651682 1105 22 2.1044685913733687 1105 23 -0.49590960186197464 1105 24 1.169361018170541 1105 25 0.746871955205286 1106 1 -1.0512630226915967 1106 2 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1107 14 -2.000125844173475 1107 15 -2.1025450739736513 1107 16 -1.671141865189476 1107 17 -4.238373355040039 1107 18 -1.479488104488098 1107 19 -2.082847047132759 1107 20 -0.11256389113530219 1107 21 -1.5979648658220142 1107 22 -0.4980955111907335 1107 23 0.016910910958536363 1107 24 -1.3691005202909992 1107 25 -6.503128044794714 1108 1 1.1941915227629494 1108 2 1.075097829859736 1108 3 1.1009348676015343 1108 4 0.48084860465811374 1108 5 0.38162833639024996 1108 6 0.7933039623515867 1108 7 1.5670017372398206 1108 8 1.566805108471769 1108 9 0.8293602341221558 1108 10 1.3305190400428444 1108 11 0.9106812059193032 1108 12 1.5400682210205972 1108 13 1.8679403488927235 1108 14 1.8670070229593918 1108 15 0.49186052043194906 1108 16 1.7973896033419905 1108 17 1.5098783932117144 1108 18 1.6533790283790353 1108 19 1.994076029790321 1108 20 0.4678556892842778 1108 21 1.8845526166954656 1108 22 1.9074988944036662 1108 23 1.1707570648046897 1108 24 0.8057246545341741 1108 25 3.496871955205286 1109 1 0.49489082346224933 1109 2 0.9911817459436492 1109 3 -0.07389030722363898 1109 4 0.4598695836790938 1109 5 0.2837262384881569 1109 6 0.33176550081312683 1109 7 -0.06936189912381252 1109 8 0.545826087492749 1109 9 -0.37343696867505116 1109 10 0.2256239351477447 1109 11 0.4001916954297897 1109 12 -1.1032884223360426 1109 13 -0.6775141965618232 1109 14 0.9229510789034521 1109 15 0.9743780029494289 1109 16 -0.13268032672794305 1109 17 1.0693189526522673 1109 18 0.5205118955119019 1109 19 -1.7751547394404454 1109 20 0.8105130319416176 1109 21 -0.36719563505278074 1109 22 0.5019044888092665 1109 23 -0.9061660121183905 1109 24 0.6308994797090008 1109 25 -0.34928189094856066 1110 1 0.19419152276294938 1110 2 0.5296432844051893 1110 3 0.4645712312379011 1110 4 0.38993951374902736 1110 5 0.017991972753890195 1110 6 0.5205766896243134 1110 7 -0.3420891718510859 1110 8 -0.06955852789186423 1110 9 -0.17063976587784424 1110 10 -0.21493550541170237 1110 11 0.09249938773748312 1110 12 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0.6239064727159942 2208 25 2.315053773387106 2209 1 0.8487369773084055 2209 2 1.1296432844051907 2209 3 1.0645712312379025 2209 4 0.7444849682944792 2209 5 1.3452647000266182 2209 6 0.8933039623515882 2209 7 0.5306381008761889 2209 8 -0.4695585278918628 2209 9 -0.2888215840596615 2209 10 0.34870085822466734 2209 11 0.6924993877374845 2209 12 0.4582500392024187 2209 13 1.4224858034381782 2209 14 1.9306433865957615 2209 15 1.7282241567955836 2209 16 2.351935057887445 2209 17 1.8462420295753503 2209 18 1.5897426647426656 2209 19 1.694076029790324 2209 20 1.9951284165570016 2209 21 2.0481889803318296 2209 22 0.8711352580400344 2209 23 0.7707570648046911 2209 24 -0.5306389818294619 2209 25 2.296871955205283 2210 1 -0.6012630226915974 2210 2 -0.3870233822614786 2210 3 -1.2020954354287667 2210 4 -2.488848365038855 2210 5 -0.6714019666400475 2210 6 -0.37336270431507756 2210 7 -2.6526952324571482 2210 8 -0.23622519455852853 2210 9 -0.5721549173930001 2210 10 -2.934632475108671 2210 11 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2213 10 1.548700858224663 2213 11 0.4924993877374817 2213 12 -0.3417499607975856 2213 13 0.4224858034381782 2213 14 0.6306433865957572 2213 15 0.828224156795585 2213 16 -0.14806494211255483 2213 17 0.7462420295753489 2213 18 -0.2102573352573316 2213 19 1.3940760297903196 2213 20 0.9951284165570016 2213 21 1.3481889803318339 2213 22 1.8711352580400344 2213 23 2.5707570648046882 2213 24 1.169361018170541 2213 25 1.3968719552052846 2214 1 -0.15126302269159808 2214 2 0.22964328440518855 2214 3 -0.3354287687620996 2214 4 0.14448496829447777 2214 5 0.6452647000266154 2214 6 0.39330396235158815 2214 7 0.8306381008761861 2214 8 0.43044147210813577 2214 9 -0.08882158405966578 2214 10 0.8487008582246673 2214 11 1.1924993877374845 2214 12 1.5582500392024201 2214 13 -0.47751419656182037 2214 14 -0.7693566134042413 2214 15 1.0282241567955808 2214 16 0.7519350578874437 2214 17 0.6462420295753475 2214 18 0.7897426647426684 2214 19 0.9940760297903211 2214 20 1.8951284165570073 2214 21 1.9481889803318353 2214 22 2.3711352580400344 2214 23 1.5707570648046882 2214 24 1.469361018170538 2214 25 2.696871955205289 2215 1 -0.7679296893582652 2215 2 -0.1370233822614786 2215 3 -2.368762102095431 2215 4 -0.6555150317055194 2215 5 -0.2547352999733832 2215 6 -2.2066960376484133 2215 7 -0.6526952324571482 2215 8 -0.48622519455852853 2215 9 -1.072154917393 2215 10 -0.5179658084419998 2215 11 -0.5741672789291812 2215 12 -2.3917499607975827 2215 13 -2.5941808632284875 2215 14 -3.2693566134042413 2215 15 -2.9551091765377535 2215 16 -3.9147316087792206 2215 17 -2.8537579704246525 2215 18 -3.0435906685906673 2215 19 -3.255923970209679 2215 20 -3.8048715834429956 2215 21 -2.5851443530015032 2215 22 -2.978864741959967 2215 23 -1.5792429351953103 2215 24 -3.6639723151627948 2215 25 -2.5864613781280497 2216 1 -1.1845963560249295 2216 2 0.19630995107185711 2216 3 -0.868762102095431 2216 4 0.8444849682944806 2216 5 0.4119313666932811 2216 6 1.1266372956849224 2216 7 1.4306381008761875 2216 8 1.5971081387748 2216 9 0.34451174927367134 2216 10 0.4820341915580002 2216 11 0.09249938773748312 2216 12 1.691583372535753 2216 13 1.6558191367715125 2216 14 -0.43602328007090563 2216 15 -1.038442509871082 2216 16 -0.24806494211255625 2216 17 -2.3537579704246525 2216 18 -1.8769240019239959 2216 19 0.16074269645698536 2216 20 -0.9715382501096599 2216 21 -0.25181101966816755 2216 22 -0.06219807529330268 2216 23 -1.162576268528646 2216 24 -0.830638981829459 2216 25 -2.003128044794714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 1.0 12 4.0 13 4.0 14 8.0 15 12.0 16 15.0 17 12.0 18 18.0 19 27.0 20 24.0 21 27.0 22 25.0 23 24.0 24 23.0 25 34.0 26 35.0 27 33.0 28 58.0 29 57.0 30 91.0 31 110.0 32 134.0 33 225.0 34 280.0 35 438.0 36 706.0 37 1280.0 38 3191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.01783384080035 20.84964477308975 13.252138610990286 24.880382775119617 2 15.035522691025083 21.690590111642745 39.06046107003045 24.213426127301727 3 18.19631723937944 25.735827171233872 29.317094388864724 26.750761200521968 4 12.933159344642597 17.456865303755258 37.75554588951719 31.854429462084966 5 12.498187617804843 37.87153835000725 35.95766275192113 13.672611280266784 6 30.332028418152817 39.71291866028708 16.61591996520226 13.339132956357835 7 25.880817746846457 32.18790778599391 23.328983616064956 18.60229085109468 8 24.097433666811657 36.69711468754531 21.21212121212121 17.99333043352182 9 22.937509061910976 16.905901116427433 22.836015658982166 37.32057416267943 10 15.021023633463827 30.18703784254024 34.05828621139626 20.73365231259968 11 32.144410613310136 26.098303610265333 23.227490213136146 18.52979556328839 12 21.32811367261128 27.925184862983905 31.11497752646078 19.631723937944034 13 25.880817746846457 24.010439321444103 27.31622444541105 22.79251848629839 14 20.980136291141076 23.256488328258662 28.621139625924314 27.142235754675948 15 23.125996810207337 30.172538784978975 24.416412933159346 22.285051471654345 16 21.93707409018414 30.78149920255183 25.286356386834857 21.99507032042917 17 20.1681890677106 30.433521821081627 28.7661302015369 20.63215890967087 18 20.675656082354646 30.01304915180513 29.404088734232275 19.907206031607945 19 21.647092938958966 28.722633028853124 30.1290416122952 19.501232419892705 20 22.578306264501162 27.305684454756378 29.350348027842227 20.765661252900234 21 22.937509061910976 29.62157459765115 26.808757430767 20.63215890967087 22 22.821516601420907 27.78019428737132 29.839060461070034 19.55922865013774 23 22.241554298970566 28.026678265912714 28.89662171958823 20.83514571552849 24 21.183123096998695 28.20066695664782 29.1866028708134 21.429607075540087 25 22.531535450195737 28.20066695664782 28.635638683485574 20.63215890967087 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 3.0 3 6.0 4 6.0 5 6.0 6 8.0 7 10.0 8 10.0 9 10.0 10 14.5 11 19.0 12 19.0 13 19.0 14 27.5 15 36.0 16 36.0 17 36.0 18 86.0 19 136.0 20 136.0 21 136.0 22 197.0 23 258.0 24 258.0 25 258.0 26 339.5 27 421.0 28 421.0 29 421.0 30 520.0 31 619.0 32 619.0 33 619.0 34 667.0 35 715.0 36 715.0 37 715.0 38 786.0 39 857.0 40 857.0 41 857.0 42 842.0 43 827.0 44 827.0 45 827.0 46 796.5 47 766.0 48 766.0 49 766.0 50 715.5 51 665.0 52 665.0 53 665.0 54 612.5 55 560.0 56 560.0 57 560.0 58 497.5 59 435.0 60 435.0 61 435.0 62 355.5 63 276.0 64 276.0 65 276.0 66 213.5 67 151.0 68 151.0 69 151.0 70 116.5 71 82.0 72 82.0 73 82.0 74 54.5 75 27.0 76 27.0 77 27.0 78 23.0 79 19.0 80 19.0 81 19.0 82 14.5 83 10.0 84 10.0 85 10.0 86 6.0 87 2.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.01449905756125852 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6897.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.57865738726983 #Duplication Level Percentage of deduplicated Percentage of total 1 98.7964338781575 96.40423372480788 2 0.950965824665676 1.8558793678410905 3 0.08915304606240713 0.26098303610265333 4 0.04457652303120357 0.17398869073510223 5 0.014858841010401188 0.0724952878062926 6 0.014858841010401188 0.08699434536755112 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.08915304606240713 1.145425547339423 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 20 0.2899811512251704 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14 0.20298680585761927 No Hit GGTATCAACGCAGAGTACTTTTTTT 13 0.18848774829636072 No Hit TATCAACGCAGAGTACTTTTTTTTT 12 0.17398869073510223 No Hit ATTCCATTCCATTCCATTCCATTCC 10 0.1449905756125852 No Hit GTGGTATCAACGCAGAGTACATGGG 10 0.1449905756125852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE