##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295214_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10650
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.036150234741783	31.0	30.0	33.0	26.0	34.0
2	30.19962441314554	31.0	30.0	33.0	26.0	34.0
3	30.380657276995304	31.0	30.0	34.0	26.0	34.0
4	33.635211267605634	35.0	35.0	37.0	28.0	37.0
5	33.89276995305164	35.0	35.0	37.0	28.0	37.0
6	33.989483568075116	36.0	35.0	37.0	29.0	37.0
7	33.88901408450704	35.0	35.0	37.0	29.0	37.0
8	33.99201877934272	36.0	35.0	37.0	29.0	37.0
9	35.137652582159625	39.0	35.0	39.0	27.0	39.0
10	35.11323943661972	39.0	35.0	39.0	27.0	39.0
11	35.49239436619718	39.0	35.0	39.0	28.0	39.0
12	35.48469483568075	39.0	35.0	39.0	28.0	39.0
13	35.471643192488266	39.0	35.0	39.0	28.0	39.0
14	36.10741784037559	39.0	36.0	41.0	27.0	41.0
15	36.497464788732394	39.0	36.0	41.0	28.0	41.0
16	36.48272300469483	39.0	36.0	41.0	27.0	41.0
17	36.18075117370892	39.0	36.0	41.0	27.0	41.0
18	36.05830985915493	39.0	36.0	41.0	27.0	41.0
19	36.472957746478876	40.0	36.0	41.0	27.0	41.0
20	36.44431924882629	40.0	36.0	41.0	27.0	41.0
21	36.552206572769954	40.0	36.0	41.0	27.0	41.0
22	36.46328638497653	39.0	36.0	41.0	27.0	41.0
23	36.11136150234742	39.0	35.0	41.0	27.0	41.0
24	36.41154929577465	39.0	36.0	41.0	27.0	41.0
25	35.23474178403756	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-3.167901935239403
1101	2	0.5233897011005162
1101	3	-0.6309818990915055
1101	4	-0.7060700979910806
1101	5	-1.3910002881862766
1101	6	0.3326915665869521
1101	7	0.1656563622600089
1101	8	-0.30643016820410196
1101	9	0.5891349147246316
1101	10	1.844647671293309
1101	11	0.5525627983850754
1101	12	-0.3443165317354513
1101	13	-0.23190112486329895
1101	14	-1.9018997302297649
1101	15	-1.205595296229987
1101	16	-0.6560289269267727
1101	17	0.35161520134982993
1101	18	0.06667876156854646
1101	19	-1.0822108644845585
1101	20	0.9567198262502927
1101	21	1.833573679679752
1101	22	1.143064995119758
1101	23	0.8160783559187621
1101	24	1.1810849736914832
1101	25	0.8280362281228619
1102	1	-1.9658817332192022
1102	2	-1.8705496928388783
1102	3	-1.358254626364232
1102	4	0.6070612151402344
1102	5	-3.158677055863045
1102	6	-2.121853887958501
1102	7	-1.9454547488511018
1102	8	-1.2205715823455137
1102	9	-0.8553095297198112
1102	10	-0.039190712545078554
1102	11	-0.42723518141290384
1102	12	-0.9301751175940396
1102	13	-1.5046283975905723
1102	14	-0.02816235649238763
1102	15	-0.2510498416845337
1102	16	-1.454008724906565
1102	17	-2.3908090410744123
1102	18	-2.3525131576233775
1102	19	-1.6832209654946624
1102	20	-2.4220680525375897
1102	21	-2.3078404617343935
1102	22	-4.250874398819633
1102	23	-0.8505883107479022
1102	24	-2.268409975803465
1102	25	-2.0962061961195673
1103	1	-1.6769928443303144
1103	2	0.06278364049445528
1103	3	0.7750787069691008
1103	4	0.11817232625134722
1103	5	1.5302118330258452
1103	6	0.8781461120414988
1103	7	0.36565636226001175
1103	8	0.12387286209893489
1103	9	0.3224682480579659
1103	10	2.0385870652327043
1103	11	1.5283203741426519
1103	12	0.5587137712948476
1103	13	0.3620382690760948
1103	14	1.4496154212853867
1103	15	1.4489501583154691
1103	16	1.9015468306489893
1103	17	0.842524292258922
1103	18	-0.3636242687344904
1103	19	0.7056679233942305
1103	20	1.9445986141290774
1103	21	-0.19672935062327923
1103	22	1.2824589345136985
1103	23	-2.6505883107479065
1103	24	-1.5461877535812434
1103	25	-0.5962061961195673
1104	1	-1.2103261776636458
1104	2	-0.5372163595055426
1104	3	-3.0249212930309
1104	4	-1.2818276737486514
1104	5	-0.7364548336408205
1104	6	-2.255187221291834
1104	7	-1.967676971073324
1104	8	-1.4761271379010665
1104	9	-3.1441984186087026
1104	10	-1.0947462681006286
1104	11	-1.0050129591906867
1104	12	-0.7079528953718182
1104	13	0.6287049357427605
1104	14	-0.15038457871461475
1104	15	-0.6177165083512008
1104	16	-1.698453169351012
1104	17	0.4425242922589234
1104	18	-0.16362426873448754
1104	19	-0.3609987432724395
1104	20	0.6779319474624117
1104	21	-0.5967293506232849
1104	22	0.08245893451369568
1104	23	-0.38392164408124074
1104	24	-1.5461877535812434
1104	25	0.203793803880437
1105	1	0.548007155669687
1105	2	-0.5163830261722104
1105	3	-0.7332546263642321
1105	4	0.8223389929180129
1105	5	0.5010451663591766
1105	6	-0.30935388795850116
1105	7	-1.896843637739991
1105	8	-2.192793804567735
1105	9	-1.0358650852753684
1105	10	0.13442039856603571
1105	11	-3.725846292524018
1105	12	-0.8329528953718182
1105	13	-0.2546283975905723
1105	14	-1.4587179120479448
1105	15	-1.2510498416845337
1105	16	-1.5026198360176792
1105	17	-2.5574757077410766
1105	18	-0.7969576020678204
1105	19	-0.8776654099391052
1105	20	-2.776234719204254
1105	21	-0.15506268395661493
1105	22	-0.8342077321529686
1105	23	0.5660783559187621
1105	24	0.4538122464187566
1105	25	1.0496271372137684
1106	1	0.09947774390498054
1106	2	0.03141109147484755
1106	3	-1.358254626364232
1106	4	-0.7328080659055161
1106	5	-0.17174895128788137
1106	6	-0.5924421232526171
1106	7	0.6362445975541249
1106	8	-0.06044086339126409
1106	9	-3.587335673510662
1106	10	0.3366262809189777
1106	11	0.16753606041715585
1106	12	-0.7079528953718182
1106	13	-0.44580486817880427
1106	14	-2.4954826179303
1106	15	-0.22163807697864968
1106	16	0.5525272228058498
1106	17	1.9131125275530394
1106	18	0.40892475087335356
1106	19	2.2546875312373658
1106	20	1.470088810207514
1106	21	0.5875843748669141
1106	22	1.0040275619646764
1106	23	0.6984312970952331
1106	24	1.5126357758305247
1106	25	2.8547741960372974
1107	1	-1.514492844330313
1107	2	-0.32888302617221044
1107	3	-0.3582546263642321
1107	4	-1.927661007081987
1107	5	-1.6239548336408234
1107	6	-1.1843538879585012
1107	7	-0.2718436377399911
1107	8	-0.005293804567735094
1107	9	0.9016349147246316
1107	10	-0.3655796014339643
1107	11	0.3366537074759819
1107	12	-1.4579528953718182
1107	13	-2.3171283975905723
1107	14	-1.7712179120479448
1107	15	-0.6885498416845337
1107	16	-2.5651198360176792
1107	17	-0.11997570774107658
1107	18	-2.2344576020678204
1107	19	-0.9401654099391052
1107	20	-0.15123471920425402
1107	21	0.34493731604338507
1107	22	-0.7717077321529686
1107	23	-0.9339216440812379
1107	24	-1.1086877535812434
1107	25	-3.6378728627862316
1108	1	-0.8547706221080915
1108	2	-0.1483274706166533
1108	3	0.5306342625246572
1108	4	0.8292834373624558
1108	5	0.007989610803619485
1108	6	-0.28852055462516546
1108	7	0.5545452511488946
1108	8	0.6683173065433792
1108	9	0.03357935916907451
1108	10	1.3496981763438143
1108	11	0.3505425963648676
1108	12	1.2364915490726247
1108	13	1.8842604912983134
1108	14	-0.41705124538128047
1108	15	0.5267279360932449
1108	16	1.8793246084267636
1108	17	0.720302070036702
1108	18	-1.2414020465122633
1108	19	-0.516554298827991
1108	20	0.4668208363513031
1108	21	0.1921595382656065
1108	22	0.8602367122914742
1108	23	1.260522800363205
1108	24	-1.1572988646923577
1108	25	-2.929539529452896
1109	1	-0.543659510996978
1109	2	-2.5372163595055426
1109	3	-0.42492129303089854
1109	4	0.31817232625134295
1109	5	-0.13645483364082622
1109	6	-0.255187221291834
1109	7	-1.3010103044066597
1109	8	-1.142793804567738
1109	9	0.12246824805796308
1109	10	-3.3614129347672943
1109	11	-3.2716796258573524
1109	12	-3.4412862287051524
1109	13	-2.3712950642572395
1109	14	-1.817051245381279
1109	15	-1.484383175017868
1109	16	-2.2317865026843435
1109	17	-0.5574757077410766
1109	18	-0.5636242687344861
1109	19	-3.4943320766057724
1109	20	-1.7887347192042569
1109	21	-4.263396017289949
1109	22	-2.984207732152967
1109	23	-4.450588310747904
1109	24	-2.146187753581245
1109	25	-0.6628728627862301
1110	1	0.9785627112252442
1110	2	1.518339196050011
1110	3	1.4195231514135465
1110	4	1.94039454847357
1110	5	2.174656277470291
1110	6	1.989257223152613
1110	7	1.4434341400377875
1110	8	2.112761750987822
1110	9	1.9224682480579673
1110	10	2.127475954121593
1110	11	2.2394314852537605
1110	12	1.5698248824059604
1110	13	2.550927157964985
1110	14	2.9718376435076124
1110	15	0.637839047204352
1110	16	2.157102386204542
1110	17	2.6091909589255877
1110	18	1.425264620154401
1110	19	1.0945568122831162
1110	20	1.411265280795746
1110	21	0.8032706493767208
1110	22	1.749125601180367
1110	23	1.260522800363205
1110	24	1.5093678019743137
1110	25	1.1260160261026542
1111	1	1.2306994633619937
1111	2	1.1038092815200962
1111	3	1.641745373635768
1111	4	2.5386851467641662
1111	5	1.5250836278976365
1111	6	1.416607650503039
1111	7	1.781040977644622
1111	8	1.3648985031245715
1111	9	2.0506733762630915
1111	10	1.725766552412189
1111	11	2.6924229382452154
1111	12	1.2920471046281818
1111	13	1.8799869870248145
1111	14	1.300897472567442
1111	15	2.2489501583154663
1111	16	0.6656493947515543
1111	17	1.8271396768743102
1111	18	3.395350090239873
1111	19	1.2954115131378146
1111	20	-0.12719625766579412
1111	21	1.5468603929664653
1111	22	1.8004076524624182
1111	23	2.8930014328418423
1111	24	2.06919686180337
1111	25	0.42943482952146184
1112	1	1.8177439977749508
1112	2	1.8487485527751595
1112	3	0.9575348473199767
1112	4	1.2269442560759103
1112	5	2.0109135874118067
1112	6	1.9307776909888688
1112	7	2.218287941207379
1112	8	1.3729956691164773
1112	9	0.6943980726193715
1112	10	0.30876250382919324
1112	11	1.2511273916865093
1112	12	1.239415525680812
1112	13	2.0743189708304826
1112	14	2.126808403741528
1112	15	0.9857922635786238
1112	16	0.8559327955612659
1112	17	1.2846295554168208
1112	18	1.8083055558269194
1112	19	0.2670714321661549
1112	20	0.4638968597431159
1112	21	0.7330952107802275
1112	22	1.9947396362680863
1112	23	-2.0260269072391353
1112	24	-0.3356614377917708
1112	25	1.1581797687927136
1113	1	1.5230071556696885
1113	2	0.29611697382778956
1113	3	0.6417453736357679
1113	4	1.2348389929180144
1113	5	0.663545166359178
1113	6	1.5781461120415017
1113	7	2.0156563622600103
1113	8	1.9072061954322663
1113	9	2.3391349147246316
1113	10	1.6219203985660329
1113	11	1.961653707475982
1113	12	1.6920471046281804
1113	13	1.9453716024094305
1113	14	2.2162820879520524
1113	15	1.5489501583154635
1113	16	2.034880163982322
1113	17	1.7425242922589206
1113	18	2.053042397932181
1113	19	1.3723345900608948
1113	20	1.6112652807957488
1113	21	2.169937316043388
1113	22	2.015792267847033
1113	23	0.36607835591875926
1113	24	0.7538122464187538
1113	25	2.1871271372137713
1114	1	0.3966913661960021
1114	2	-0.04598828933010424
1114	3	-0.25299146846949583
1114	4	0.2269442560759103
1114	5	0.010913587411806702
1114	6	-0.016590730063761328
1114	7	-0.30802784826630614
1114	8	-0.15332012035720766
1114	9	-1.042444032643786
1114	10	-0.7438690751181767
1114	11	-2.538346292524018
1114	12	0.6604681572597642
1114	13	-0.13620734495899
1114	14	1.0741768247941579
1114	15	0.8278975267365212
1114	16	-1.0388040465439943
1114	17	1.179366397522081
1114	18	0.2819897663532345
1114	19	0.6354924847977372
1114	20	0.6744231755325885
1114	21	2.1541478423591727
1114	22	1.047371215215449
1114	23	1.1318678296029745
1114	24	1.1906543516819141
1114	25	-1.999714968049389
1115	1	1.6288895086108646
1115	2	1.5608228561807316
1115	3	1.524098314812239
1115	4	2.502486051741542
1115	5	1.4753098722415317
1115	6	2.231087288512086
1115	7	1.6362445975541249
1115	8	1.46897090131462
1115	9	1.4126643264893346
1115	10	2.2778027515072097
1115	11	1.226359589828924
1115	12	2.409694163451711
1115	13	1.4953716024094277
1115	14	2.2103997350108813
1115	15	1.0136560406684083
1115	16	0.6701742816293788
1115	17	1.9719360569648074
1115	18	2.5853953391086506
1115	19	2.1958640018255977
1115	20	1.94067704550163
1115	21	1.175819668984559
1115	22	0.004027561964676352
1115	23	1.933725414742291
1115	24	2.1596945993599306
1115	25	2.4430094901549424
1116	1	1.173007155669687
1116	2	1.0961169738277903
1116	3	1.691745373635765
1116	4	1.8348389929180158
1116	5	1.8135451663591766
1116	6	2.2781461120414974
1116	7	0.7156563622600061
1116	8	0.7572061954322677
1116	9	1.3891349147246288
1116	10	-0.9280796014339643
1116	11	-0.6883462925240167
1116	12	0.04204710462818184
1116	13	1.1953716024094305
1116	14	1.6662820879520552
1116	15	1.7989501583154635
1116	16	1.484880163982318
1116	17	0.8925242922589263
1116	18	2.303042397932181
1116	19	0.4723345900608962
1116	20	0.7612652807957474
1116	21	1.669937316043388
1116	22	0.4157922678470314
1116	23	1.066078355918762
1116	24	1.4038122464187595
1116	25	1.137127137213767
1201	1	-3.201992844330313
1201	2	-1.9538830261722104
1201	3	-1.733254626364232
1201	4	-1.240161007081987
1201	5	-2.8739548336408234
1201	6	-3.309353887958501
1201	7	-2.584343637739991
1201	8	-2.005293804567735
1201	9	-0.6608650852753684
1201	10	0.6344203985660357
1201	11	-2.225846292524018
1201	12	1.1045471046281818
1201	13	0.7453716024094277
1201	14	0.10378208795205524
1201	15	0.3114501583154663
1201	16	-1.1276198360176792
1201	17	1.5675242922589234
1201	18	-0.8594576020678204
1201	19	-1.0651654099391052
1201	20	-1.026234719204254
1201	21	-1.592562683956615
1201	22	-0.2717077321529686
1201	23	1.566078355918762
1201	24	-1.7961877535812434
1201	25	1.9246271372137684
1202	1	-0.7198499871874553
1202	2	-2.4181687404579257
1202	3	-1.858254626364232
1202	4	-3.615161007081987
1202	5	-1.1507405479265387
1202	6	-1.0504253165299318
1202	7	-2.262915066311418
1202	8	-3.0856509474248774
1202	9	-3.2680079424182225
1202	10	-3.5709367442911066
1202	11	-3.8954891496668758
1202	12	-3.707952895371818
1202	13	-5.290342683304857
1202	14	-7.583717912047945
1202	15	-4.251049841684534
1202	16	-5.065119836017679
1202	17	-2.771761422026792
1202	18	-0.9398147449249663
1202	19	-3.8419511242248205
1202	20	-5.803020433489969
1202	21	-2.3157769696708996
1202	22	-3.3699220178672533
1202	23	-4.398207358366953
1202	24	-1.7604734678669587
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2212	4	-2.615161007081987
2212	5	-0.7785600967987207
2212	6	-0.5955380984848162
2212	7	-2.0975015324768336
2212	8	0.4256272480638472
2212	9	-0.9371808747490533
2212	10	-0.7438690751181767
2212	11	-1.0120305030503332
2212	12	-2.0237423690560306
2212	13	-0.3467336607484697
2212	14	-0.5047705436268899
2212	15	-2.540523525895061
2212	16	-2.3545935202282067
2212	17	-1.873265181425289
2212	18	-1.2443260231204505
2212	19	-3.20661277836016
2212	20	-0.7992610349937266
2212	21	-0.3721679471145123
2212	22	1.573687004689134
2212	23	-0.23655322302860782
2212	24	-0.5988193325286133
2212	25	0.5266008214242959
2213	1	0.4524189203755711
2213	2	1.2667052091219055
2213	3	0.4064512559887099
2213	4	0.26719193409448394
2213	5	0.5341334016532926
2213	6	-2.1806774173702657
2213	7	-1.2461083436223461
2213	8	-2.178087922214793
2213	9	-1.1755709676283104
2213	10	-0.6045501896692613
2213	11	-0.47952276311225006
2213	12	-0.9432470130188761
2213	13	-0.9752166328846883
2213	14	1.0927526761873523
2213	15	-0.9863439593315917
2213	16	-1.0357080713117952
2213	17	-0.9692404136234316
2213	18	1.1148071038145346
2213	19	0.25468753123736576
2213	20	1.587735869031043
2213	21	0.7640549631022111
2213	22	0.7687334443176184
2213	23	0.5219607088599361
2213	24	1.2773416581834596
2213	25	0.2077153725078844
2214	1	0.7325309651934973
2214	2	1.41516459287541
2214	3	0.49888823077862554
2214	4	1.241981850060867
2214	5	0.682592785406797
2214	6	0.9733842072795937
2214	7	0.4513706479742936
2214	8	0.4381585763846445
2214	9	1.3510396766293908
2214	10	0.6195394461850867
2214	11	1.5568918027140768
2214	12	-0.46985765727657736
2214	13	0.2572763643141869
2214	14	1.0591392308092011
2214	15	1.7727596821249918
2214	16	1.0063087354108902
2214	17	0.823476673211303
2214	18	1.7506614455512306
2214	19	1.5151917329180407
2214	20	1.9350748046052715
2214	21	0.42231826842433406
2214	22	1.2253160773708416
2214	23	0.7208402606806672
2214	24	-0.5461877535812434
2214	25	0.49903189911852763
2215	1	1.3991976318601616
2215	2	0.27230745001826406
2215	3	-0.02492129303089996
2215	4	1.6705532786322976
2215	5	0.682592785406797
2215	6	1.0686223025176886
2215	7	1.3561325527361987
2215	8	1.7714919097179802
2215	9	1.208182533772252
2215	10	1.6195394461850867
2215	11	1.7949870408093176
2215	12	0.8634756760567512
2215	13	0.2572763643141869
2215	14	0.05913923080920114
2215	15	0.7727596821249918
2215	16	0.6253563544585106
2215	17	0.871095720830354
2215	18	0.8458995407893255
2215	19	-0.91337969565339
2215	20	-2.8744490049185387
2215	21	-3.530062683956615
2215	22	-1.3937315416767788
2215	23	0.6256021654425723
2215	24	-0.4509496583431485
2215	25	-1.1676347675481367
2216	1	-0.07699284433031295
2216	2	-1.8038830261722119
2216	3	-0.8249212930308971
2216	4	-1.615161007081987
2216	5	0.5968784996925081
2216	6	0.14481277870816456
2216	7	0.2323230289266789
2216	8	-0.3427938045677337
2216	9	-0.6775317519420341
2216	10	-2.0947462681006286
2216	11	-0.07167962585734955
2216	12	-0.841286228705151
2216	13	-1.3046283975905695
2216	14	-3.017051245381275
2216	15	-2.617716508351201
2216	16	-0.2984531693510135
2216	17	-1.3574757077410737
2216	18	-0.43029093540115326
2216	19	-2.094332076605774
2216	20	-2.9220680525375897
2216	21	-2.596729350623285
2216	22	-3.050874398819637
2216	23	-5.317254977414571
2216	24	-5.746187753581243
2216	25	-6.062872862786232
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19.0
3	4.0
4	1.0
5	6.0
6	3.0
7	4.0
8	3.0
9	1.0
10	2.0
11	13.0
12	22.0
13	28.0
14	37.0
15	43.0
16	55.0
17	54.0
18	66.0
19	68.0
20	51.0
21	64.0
22	69.0
23	76.0
24	69.0
25	90.0
26	94.0
27	121.0
28	141.0
29	175.0
30	228.0
31	262.0
32	294.0
33	443.0
34	610.0
35	825.0
36	1215.0
37	1987.0
38	3407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43085456425684	21.61323681489142	13.180408009777192	23.775500611074552
2	15.798888993503438	21.85293286884474	40.07155635062612	22.276621787025704
3	17.8631995477671	26.370830977953645	30.177124552477856	25.588844921801396
4	12.436356779181596	17.82010182915331	39.09108052046012	30.65246087120498
5	13.706636500754149	38.04675716440422	34.71907993966818	13.527526395173453
6	31.027332704995285	37.80395852968898	18.162111215834116	13.006597549481622
7	24.884532001131117	33.122820246960124	24.432085964746914	17.560561787161845
8	23.190045248868778	36.18966817496229	22.633861236802414	17.986425339366516
9	24.401508011310085	17.313854853911405	21.894439208294063	36.39019792648445
10	14.719471947194721	30.41018387553041	33.61621876473362	21.254125412541256
11	31.66023166023166	25.76513796025991	24.27723891138525	18.297391468123177
12	19.943449575871817	28.2186616399623	32.620169651272384	19.217719132893496
13	26.890835452576056	24.602053310728078	25.85476123198644	22.65235000470943
14	19.694685261967585	24.26498303807011	28.486618921975122	27.553712777987183
15	22.14366515837104	32.343514328808446	24.066742081447963	21.44607843137255
16	21.781712025614464	32.05574912891986	25.58621339109144	20.576325454374235
17	20.1263197586727	30.684389140271495	28.82730015082956	20.361990950226243
18	19.51954780970325	31.05982100800754	29.251059821008006	20.169571361281204
19	21.33634907171803	28.649514654603713	28.988785222881912	21.025351050796342
20	21.480365382804408	28.9857802052924	29.089368113758358	20.444486298144835
21	23.08634992458522	29.166666666666668	27.413273001508298	20.33371040723982
22	21.96798493408663	28.380414312617702	29.67043314500942	19.981167608286253
23	22.340425531914892	28.186782150254192	28.233854264733573	21.238938053097346
24	21.125035334024307	27.69245265240742	29.350796193347783	21.831715820220484
25	21.780499293452664	28.911917098445596	28.0357983984927	21.271785209609043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	23.0
2	15.5
3	8.0
4	8.0
5	8.0
6	21.5
7	35.0
8	35.0
9	35.0
10	36.0
11	37.0
12	37.0
13	37.0
14	65.0
15	93.0
16	93.0
17	93.0
18	157.5
19	222.0
20	222.0
21	222.0
22	319.5
23	417.0
24	417.0
25	417.0
26	532.0
27	647.0
28	647.0
29	647.0
30	789.0
31	931.0
32	931.0
33	931.0
34	1065.0
35	1199.0
36	1199.0
37	1199.0
38	1251.5
39	1304.0
40	1304.0
41	1304.0
42	1276.0
43	1248.0
44	1248.0
45	1248.0
46	1200.0
47	1152.0
48	1152.0
49	1152.0
50	1123.0
51	1094.0
52	1094.0
53	1094.0
54	962.0
55	830.0
56	830.0
57	830.0
58	731.5
59	633.0
60	633.0
61	633.0
62	513.5
63	394.0
64	394.0
65	394.0
66	296.5
67	199.0
68	199.0
69	199.0
70	155.5
71	112.0
72	112.0
73	112.0
74	76.0
75	40.0
76	40.0
77	40.0
78	30.0
79	20.0
80	20.0
81	20.0
82	14.5
83	9.0
84	9.0
85	9.0
86	5.5
87	2.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12206572769953052
2	0.27230046948356806
3	0.3380281690140845
4	0.41314553990610325
5	0.39436619718309857
6	0.3755868544600939
7	0.38497652582159625
8	0.39436619718309857
9	0.3755868544600939
10	0.42253521126760557
11	0.29107981220657275
12	0.3755868544600939
13	0.30985915492957744
14	0.3568075117370892
15	0.39436619718309857
16	0.29107981220657275
17	0.39436619718309857
18	0.32863849765258213
19	0.36619718309859156
20	0.29107981220657275
21	0.39436619718309857
22	0.28169014084507044
23	0.2629107981220657
24	0.3474178403755869
25	0.32863849765258213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	10650.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.76525821596243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.62658470995007	96.4225352112676
2	1.114099116404149	2.1784037558685445
3	0.12485593545908567	0.36619718309859156
4	0.01920860545524395	0.07511737089201878
5	0.01920860545524395	0.09389671361502347
6	0.009604302727621975	0.056338028169014086
7	0.01920860545524395	0.13145539906103285
8	0.009604302727621975	0.07511737089201878
9	0.01920860545524395	0.16901408450704225
>10	0.0384172109104879	0.431924882629108
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	14	0.13145539906103285	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	11	0.10328638497652581	No Hit
GAATGGAATGGAATGGAATGGAATG	11	0.10328638497652581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE